| UniProt ID | TRI25_HUMAN | |
|---|---|---|
| UniProt AC | Q14258 | |
| Protein Name | E3 ubiquitin/ISG15 ligase TRIM25 | |
| Gene Name | TRIM25 | |
| Organism | Homo sapiens (Human). | |
| Sequence Length | 630 | |
| Subcellular Localization | Cytoplasm . Colocalized with DDX58 at cytoplasmic perinuclear bodies. | |
| Protein Description | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. [PubMed: 22452784] | |
| Protein Sequence | MAELCPLAEELSCSICLEPFKEPVTTPCGHNFCGSCLNETWAVQGSPYLCPQCRAVYQARPQLHKNTVLCNVVEQFLQADLAREPPADVWTPPARASAPSPNAQVACDHCLKEAAVKTCLVCMASFCQEHLQPHFDSPAFQDHPLQPPVRDLLRRKCSQHNRLREFFCPEHSECICHICLVEHKTCSPASLSQASADLEATLRHKLTVMYSQINGASRALDDVRNRQQDVRMTANRKVEQLQQEYTEMKALLDASETTSTRKIKEEEKRVNSKFDTIYQILLKKKSEIQTLKEEIEQSLTKRDEFEFLEKASKLRGISTKPVYIPEVELNHKLIKGIHQSTIDLKNELKQCIGRLQEPTPSSGDPGEHDPASTHKSTRPVKKVSKEEKKSKKPPPVPALPSKLPTFGAPEQLVDLKQAGLEAAAKATSSHPNSTSLKAKVLETFLAKSRPELLEYYIKVILDYNTAHNKVALSECYTVASVAEMPQNYRPHPQRFTYCSQVLGLHCYKKGIHYWEVELQKNNFCGVGICYGSMNRQGPESRLGRNSASWCVEWFNTKISAWHNNVEKTLPSTKATRVGVLLNCDHGFVIFFAVADKVHLMYKFRVDFTEALYPAFWVFSAGATLSICSPK | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 12 | Phosphorylation | CPLAEELSCSICLEP CHHHHHHCCEEECCC | 14.75 | - | |
| 46 | Phosphorylation | ETWAVQGSPYLCPQC CCCCCCCCCCCCHHH | 8.03 | 25159151 | |
| 48 | Phosphorylation | WAVQGSPYLCPQCRA CCCCCCCCCCHHHHH | 23.72 | 22817900 | |
| 57 | Phosphorylation | CPQCRAVYQARPQLH CHHHHHHHHHCCCCC | 8.75 | 22817900 | |
| 65 | Ubiquitination | QARPQLHKNTVLCNV HHCCCCCCCCHHHHH | 64.01 | 33845483 | |
| 67 | Phosphorylation | RPQLHKNTVLCNVVE CCCCCCCCHHHHHHH | 21.47 | 20873877 | |
| 70 | Glutathionylation | LHKNTVLCNVVEQFL CCCCCHHHHHHHHHH | 2.93 | 22555962 | |
| 91 | Phosphorylation | EPPADVWTPPARASA CCCCCCCCCCCCCCC | 21.17 | 25159151 | |
| 97 | Phosphorylation | WTPPARASAPSPNAQ CCCCCCCCCCCCCHH | 34.85 | 29255136 | |
| 100 | Phosphorylation | PARASAPSPNAQVAC CCCCCCCCCCHHHHH | 30.42 | 29255136 | |
| 112 | 2-Hydroxyisobutyrylation | VACDHCLKEAAVKTC HHHHHHHHHHHHHHH | 51.18 | - | |
| 112 | Ubiquitination | VACDHCLKEAAVKTC HHHHHHHHHHHHHHH | 51.18 | 32015554 | |
| 117 | Ubiquitination | CLKEAAVKTCLVCMA HHHHHHHHHHHHHHH | 28.88 | 21963094 | |
| 125 | Phosphorylation | TCLVCMASFCQEHLQ HHHHHHHHHHHHHCC | 10.32 | 28348404 | |
| 137 | Phosphorylation | HLQPHFDSPAFQDHP HCCCCCCCHHHCCCC | 19.72 | 28348404 | |
| 172 | Phosphorylation | EFFCPEHSECICHIC HHHCCCCCCEEEEEE | 32.28 | 27080861 | |
| 185 | Phosphorylation | ICLVEHKTCSPASLS EEEEECCCCCHHHHH | 21.81 | 21815630 | |
| 187 | Phosphorylation | LVEHKTCSPASLSQA EEECCCCCHHHHHHH | 28.82 | 21815630 | |
| 190 | Phosphorylation | HKTCSPASLSQASAD CCCCCHHHHHHHCCC | 31.75 | 21815630 | |
| 192 | Phosphorylation | TCSPASLSQASADLE CCCHHHHHHHCCCHH | 22.62 | 27050516 | |
| 205 | Ubiquitination | LEATLRHKLTVMYSQ HHHHHHHHHHHHHHH | 39.14 | 21906983 | |
| 237 | Acetylation | VRMTANRKVEQLQQE HHHHHHHHHHHHHHH | 50.35 | 23236377 | |
| 237 | Malonylation | VRMTANRKVEQLQQE HHHHHHHHHHHHHHH | 50.35 | 26320211 | |
| 237 | Ubiquitination | VRMTANRKVEQLQQE HHHHHHHHHHHHHHH | 50.35 | 22817900 | |
| 245 | Phosphorylation | VEQLQQEYTEMKALL HHHHHHHHHHHHHHH | 12.03 | 28796482 | |
| 246 | Phosphorylation | EQLQQEYTEMKALLD HHHHHHHHHHHHHHH | 28.93 | 28796482 | |
| 249 | Ubiquitination | QQEYTEMKALLDASE HHHHHHHHHHHHHCC | 29.65 | 21906983 | |
| 255 | Phosphorylation | MKALLDASETTSTRK HHHHHHHCCCCCCHH | 34.83 | 21406692 | |
| 257 | Phosphorylation | ALLDASETTSTRKIK HHHHHCCCCCCHHHH | 24.45 | 21406692 | |
| 258 | Phosphorylation | LLDASETTSTRKIKE HHHHCCCCCCHHHHH | 24.85 | 21406692 | |
| 259 | Phosphorylation | LDASETTSTRKIKEE HHHCCCCCCHHHHHH | 33.37 | 21406692 | |
| 260 | Phosphorylation | DASETTSTRKIKEEE HHCCCCCCHHHHHHH | 33.61 | 21406692 | |
| 268 | Ubiquitination | RKIKEEEKRVNSKFD HHHHHHHHHHHHHHH | 66.00 | 23000965 | |
| 272 | Phosphorylation | EEEKRVNSKFDTIYQ HHHHHHHHHHHHHHH | 31.66 | 28152594 | |
| 273 | Acetylation | EEKRVNSKFDTIYQI HHHHHHHHHHHHHHH | 41.89 | 19608861 | |
| 273 | Ubiquitination | EEKRVNSKFDTIYQI HHHHHHHHHHHHHHH | 41.89 | 23000965 | |
| 276 | Phosphorylation | RVNSKFDTIYQILLK HHHHHHHHHHHHHHH | 25.39 | 21945579 | |
| 278 | Phosphorylation | NSKFDTIYQILLKKK HHHHHHHHHHHHHCH | 7.50 | 21945579 | |
| 283 | 2-Hydroxyisobutyrylation | TIYQILLKKKSEIQT HHHHHHHHCHHHHHH | 55.50 | - | |
| 283 | Acetylation | TIYQILLKKKSEIQT HHHHHHHHCHHHHHH | 55.50 | 26051181 | |
| 283 | Methylation | TIYQILLKKKSEIQT HHHHHHHHCHHHHHH | 55.50 | 24633609 | |
| 283 | Ubiquitination | TIYQILLKKKSEIQT HHHHHHHHCHHHHHH | 55.50 | 21906983 | |
| 284 | Methylation | IYQILLKKKSEIQTL HHHHHHHCHHHHHHH | 62.59 | 24758723 | |
| 284 | Ubiquitination | IYQILLKKKSEIQTL HHHHHHHCHHHHHHH | 62.59 | 22817900 | |
| 285 | Ubiquitination | YQILLKKKSEIQTLK HHHHHHCHHHHHHHH | 52.30 | 33845483 | |
| 286 | Phosphorylation | QILLKKKSEIQTLKE HHHHHCHHHHHHHHH | 49.14 | 21406692 | |
| 290 | Phosphorylation | KKKSEIQTLKEEIEQ HCHHHHHHHHHHHHH | 45.52 | 21406692 | |
| 292 | Acetylation | KSEIQTLKEEIEQSL HHHHHHHHHHHHHHC | 57.60 | 26051181 | |
| 292 | Ubiquitination | KSEIQTLKEEIEQSL HHHHHHHHHHHHHHC | 57.60 | 21906983 | |
| 301 | 2-Hydroxyisobutyrylation | EIEQSLTKRDEFEFL HHHHHCCCHHHHHHH | 64.24 | - | |
| 301 | Ubiquitination | EIEQSLTKRDEFEFL HHHHHCCCHHHHHHH | 64.24 | 21906983 | |
| 310 | 2-Hydroxyisobutyrylation | DEFEFLEKASKLRGI HHHHHHHHHHHHCCC | 61.17 | - | |
| 310 | Ubiquitination | DEFEFLEKASKLRGI HHHHHHHHHHHHCCC | 61.17 | 21906983 | |
| 312 | Phosphorylation | FEFLEKASKLRGIST HHHHHHHHHHCCCCC | 43.20 | - | |
| 313 | Ubiquitination | EFLEKASKLRGISTK HHHHHHHHHCCCCCC | 47.25 | 22817900 | |
| 318 | Phosphorylation | ASKLRGISTKPVYIP HHHHCCCCCCCEECC | 32.95 | 28152594 | |
| 319 | Phosphorylation | SKLRGISTKPVYIPE HHHCCCCCCCEECCH | 37.13 | 28152594 | |
| 320 | Acetylation | KLRGISTKPVYIPEV HHCCCCCCCEECCHH | 26.20 | 19608861 | |
| 320 | Malonylation | KLRGISTKPVYIPEV HHCCCCCCCEECCHH | 26.20 | 26320211 | |
| 320 | Ubiquitination | KLRGISTKPVYIPEV HHCCCCCCCEECCHH | 26.20 | 23000965 | |
| 323 | Phosphorylation | GISTKPVYIPEVELN CCCCCCEECCHHHHC | 21.20 | 28152594 | |
| 332 | Acetylation | PEVELNHKLIKGIHQ CHHHHCHHHHHCHHH | 51.32 | 25953088 | |
| 332 | Ubiquitination | PEVELNHKLIKGIHQ CHHHHCHHHHHCHHH | 51.32 | 32015554 | |
| 335 | Acetylation | ELNHKLIKGIHQSTI HHCHHHHHCHHHHHH | 63.35 | 23236377 | |
| 335 | Malonylation | ELNHKLIKGIHQSTI HHCHHHHHCHHHHHH | 63.35 | 26320211 | |
| 335 | Ubiquitination | ELNHKLIKGIHQSTI HHCHHHHHCHHHHHH | 63.35 | 22817900 | |
| 340 | Phosphorylation | LIKGIHQSTIDLKNE HHHCHHHHHHHHHHH | 17.11 | 23312004 | |
| 341 | Phosphorylation | IKGIHQSTIDLKNEL HHCHHHHHHHHHHHH | 16.37 | 23312004 | |
| 345 | Acetylation | HQSTIDLKNELKQCI HHHHHHHHHHHHHHH | 44.63 | 26822725 | |
| 345 | Ubiquitination | HQSTIDLKNELKQCI HHHHHHHHHHHHHHH | 44.63 | 23000965 | |
| 349 | Acetylation | IDLKNELKQCIGRLQ HHHHHHHHHHHHHHC | 36.62 | 25953088 | |
| 349 | Malonylation | IDLKNELKQCIGRLQ HHHHHHHHHHHHHHC | 36.62 | 26320211 | |
| 349 | Ubiquitination | IDLKNELKQCIGRLQ HHHHHHHHHHHHHHC | 36.62 | 23000965 | |
| 372 | Phosphorylation | PGEHDPASTHKSTRP CCCCCCCCCCCCCCC | 36.11 | 28348404 | |
| 373 | Phosphorylation | GEHDPASTHKSTRPV CCCCCCCCCCCCCCC | 35.11 | 28348404 | |
| 388 | Ubiquitination | KKVSKEEKKSKKPPP CCCCHHHHHCCCCCC | 64.54 | 22817900 | |
| 389 | Ubiquitination | KVSKEEKKSKKPPPV CCCHHHHHCCCCCCC | 71.44 | 22817900 | |
| 391 | Malonylation | SKEEKKSKKPPPVPA CHHHHHCCCCCCCCC | 77.50 | 26320211 | |
| 391 | Ubiquitination | SKEEKKSKKPPPVPA CHHHHHCCCCCCCCC | 77.50 | 22817900 | |
| 392 | Ubiquitination | KEEKKSKKPPPVPAL HHHHHCCCCCCCCCC | 70.60 | 21906983 | |
| 401 | Phosphorylation | PPVPALPSKLPTFGA CCCCCCCCCCCCCCC | 47.91 | 24719451 | |
| 402 | Acetylation | PVPALPSKLPTFGAP CCCCCCCCCCCCCCC | 57.45 | 23954790 | |
| 402 | Ubiquitination | PVPALPSKLPTFGAP CCCCCCCCCCCCCCC | 57.45 | 21906983 | |
| 405 | Phosphorylation | ALPSKLPTFGAPEQL CCCCCCCCCCCCHHH | 45.17 | 27251275 | |
| 416 | Methylation | PEQLVDLKQAGLEAA CHHHCHHHHHHHHHH | 33.37 | 24763869 | |
| 416 | Ubiquitination | PEQLVDLKQAGLEAA CHHHCHHHHHHHHHH | 33.37 | 21906983 | |
| 425 | Methylation | AGLEAAAKATSSHPN HHHHHHHHHCCCCCC | 47.69 | 24751691 | |
| 425 | Ubiquitination | AGLEAAAKATSSHPN HHHHHHHHHCCCCCC | 47.69 | 21906983 | |
| 427 | Phosphorylation | LEAAAKATSSHPNST HHHHHHHCCCCCCCH | 30.01 | 23403867 | |
| 428 | Phosphorylation | EAAAKATSSHPNSTS HHHHHHCCCCCCCHH | 31.35 | 23403867 | |
| 429 | Phosphorylation | AAAKATSSHPNSTSL HHHHHCCCCCCCHHH | 38.31 | 23403867 | |
| 433 | Phosphorylation | ATSSHPNSTSLKAKV HCCCCCCCHHHHHHH | 24.67 | 25159151 | |
| 434 | Phosphorylation | TSSHPNSTSLKAKVL CCCCCCCHHHHHHHH | 44.72 | 25159151 | |
| 435 | Phosphorylation | SSHPNSTSLKAKVLE CCCCCCHHHHHHHHH | 27.91 | 25159151 | |
| 437 | Ubiquitination | HPNSTSLKAKVLETF CCCCHHHHHHHHHHH | 46.02 | 22817900 | |
| 439 | Ubiquitination | NSTSLKAKVLETFLA CCHHHHHHHHHHHHH | 45.85 | 21906983 | |
| 443 | Phosphorylation | LKAKVLETFLAKSRP HHHHHHHHHHHHCCH | 21.56 | 20068231 | |
| 447 | Acetylation | VLETFLAKSRPELLE HHHHHHHHCCHHHHH | 49.50 | 26051181 | |
| 447 | Ubiquitination | VLETFLAKSRPELLE HHHHHHHHCCHHHHH | 49.50 | 29967540 | |
| 455 | Phosphorylation | SRPELLEYYIKVILD CCHHHHHHHHHHHHC | 15.51 | 28152594 | |
| 456 | Phosphorylation | RPELLEYYIKVILDY CHHHHHHHHHHHHCC | 5.72 | 28152594 | |
| 463 | Phosphorylation | YIKVILDYNTAHNKV HHHHHHCCCCCCCEE | 15.80 | 28152594 | |
| 465 | Phosphorylation | KVILDYNTAHNKVAL HHHHCCCCCCCEEEH | 24.06 | 28152594 | |
| 496 | Phosphorylation | RPHPQRFTYCSQVLG CCCCCHHCHHHHHHH | 26.30 | 28152594 | |
| 497 | Phosphorylation | PHPQRFTYCSQVLGL CCCCHHCHHHHHHHC | 6.33 | 28152594 | |
| 499 | Phosphorylation | PQRFTYCSQVLGLHC CCHHCHHHHHHHCHH | 16.95 | 28152594 | |
| 508 | 2-Hydroxyisobutyrylation | VLGLHCYKKGIHYWE HHHCHHCCCCCCEEE | 50.33 | - | |
| 508 | Acetylation | VLGLHCYKKGIHYWE HHHCHHCCCCCCEEE | 50.33 | 25953088 | |
| 508 | Ubiquitination | VLGLHCYKKGIHYWE HHHCHHCCCCCCEEE | 50.33 | 22817900 | |
| 509 | Acetylation | LGLHCYKKGIHYWEV HHCHHCCCCCCEEEE | 36.30 | 26051181 | |
| 509 | Malonylation | LGLHCYKKGIHYWEV HHCHHCCCCCCEEEE | 36.30 | 26320211 | |
| 509 | Ubiquitination | LGLHCYKKGIHYWEV HHCHHCCCCCCEEEE | 36.30 | 22817900 | |
| 530 | Phosphorylation | FCGVGICYGSMNRQG EEEEEEEECCCCCCC | 15.43 | 20873877 | |
| 532 | Phosphorylation | GVGICYGSMNRQGPE EEEEEECCCCCCCCC | 6.09 | 20873877 | |
| 546 | Phosphorylation | ESRLGRNSASWCVEW CHHCCCCCHHHHHHH | 23.48 | 27251275 | |
| 548 | Phosphorylation | RLGRNSASWCVEWFN HCCCCCHHHHHHHHH | 22.34 | 27251275 | |
| 567 | Acetylation | AWHNNVEKTLPSTKA HHHCCHHHHCCCCCC | 51.59 | 19608861 | |
| 567 | Malonylation | AWHNNVEKTLPSTKA HHHCCHHHHCCCCCC | 51.59 | 26320211 | |
| 567 | Ubiquitination | AWHNNVEKTLPSTKA HHHCCHHHHCCCCCC | 51.59 | 22817900 | |
| 572 | Phosphorylation | VEKTLPSTKATRVGV HHHHCCCCCCCEEEE | 23.82 | 29496963 | |
| 573 | Ubiquitination | EKTLPSTKATRVGVL HHHCCCCCCCEEEEE | 52.69 | - | |
| 628 | Phosphorylation | GATLSICSPK----- CCEEEECCCC----- | 33.77 | 29460479 |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
| 12 | S | Phosphorylation | Kinase | LZK | O43283 | PSP |
| 278 | Y | Phosphorylation | Kinase | SRC | P12931 | PSP |
| - | K | Ubiquitination | E3 ubiquitin ligase | RBCK1 | Q9BYM8 | PMID:21292167 |
| - | K | Ubiquitination | E3 ubiquitin ligase | RNF31 | Q96EP0 | PMID:21292167 |
| - | K | Ubiquitination | E3 ubiquitin ligase | TRIM25 | Q14258 | PMID:22199232 |
| Modified Location | Modified Residue | Modification | Function | Reference | ||
|---|---|---|---|---|---|---|
Oops, there are no descriptions of PTM sites of TRI25_HUMAN !! | ||||||
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of TRI25_HUMAN !! | ||||||
| Kegg Disease | ||||||
|---|---|---|---|---|---|---|
| There are no disease associations of PTM sites. | ||||||
| OMIM Disease | ||||||
| There are no disease associations of PTM sites. | ||||||
| Kegg Drug | ||||||
| There are no disease associations of PTM sites. | ||||||
| DrugBank | ||||||
| There are no disease associations of PTM sites. | ||||||
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| Acetylation | |
| Reference | PubMed |
| "Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-273; LYS-320 AND LYS-567,AND MASS SPECTROMETRY. | |
| Phosphorylation | |
| Reference | PubMed |
| "A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-91 AND SER-100, AND MASSSPECTROMETRY. | |
| "Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."; Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.; Anal. Sci. 24:161-166(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, AND MASSSPECTROMETRY. | |
| "Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-278, AND MASSSPECTROMETRY. | |