dbPTM

MILK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MILK1_HUMAN
UniProt AC	Q8N3F8
Protein Name	MICAL-like protein 1
Gene Name	MICALL1
Organism	Homo sapiens (Human).
Sequence Length	863
Subcellular Localization	Recycling endosome membrane Peripheral membrane protein. Late endosome membrane. Localization to late endosomes is actin-dependent. Association to tubular recycling endosomes is regulated by RAB35 and ARF6.
Protein Description	Probable lipid-binding protein with higher affinity for phosphatidic acid, a lipid enriched in recycling endosome membranes. On endosome membranes, may act as a downstream effector of Rab proteins recruiting cytosolic proteins to regulate membrane tubulation. May be involved in a late step of receptor-mediated endocytosis regulating for instance endocytosed-EGF receptor trafficking. Alternatively, may regulate slow endocytic recycling of endocytosed proteins back to the plasma membrane. May indirectly play a role in neurite outgrowth..
Protein Sequence	MAGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDVAQGEELSSGSLSEQGTGQTPSSTCAACQQHVHLVQRYLADGRLYHRHCFRCRRCSSTLLPGAYENGPEEGTFVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQLAEDAKDVPGGGPSSSAPAGAEADGPKASPEARPQIPTKPRVPGKLQELASPPAGRPTPAPRKASESTTPAPPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVPKPRGTPKPSEGTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPSQDSRQVENGGTEEVAQPSPTASLESKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSATPSPALSVESLSSESASQTAGAELLEPPAVPKSSSEPAVHAPGTPGNPVSLSTNSSLASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAKPCSGATPTPLLLVGDRSPVPSPGSSSPQLQVKSSCKENPFNRKPSPAASPATKKATKGSKPVRPPAPGHGFPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEHRGVLLEEKLRGGLNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDEDRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDAKSKSPRDKS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
182	Methylation	QRYLADGRLYHRHCF HHHHCCCCEECCCCC	32.40	115483307
195	Phosphorylation	CFRCRRCSSTLLPGA CCCCCCCCCCCCCCC	25.25	23927012
196	Phosphorylation	FRCRRCSSTLLPGAY CCCCCCCCCCCCCCH	27.05	26657352
197	Phosphorylation	RCRRCSSTLLPGAYE CCCCCCCCCCCCCHH	18.93	28450419
203	Phosphorylation	STLLPGAYENGPEEG CCCCCCCHHCCCCCC	18.82	23927012
227	Phosphorylation	RLGPGTRSGTRPGPF HCCCCCCCCCCCCCC	44.33	30576142
229	Phosphorylation	GPGTRSGTRPGPFSQ CCCCCCCCCCCCCCC	34.63	30576142
235	Phosphorylation	GTRPGPFSQPKQQHQ CCCCCCCCCHHHHHH	49.02	28555341
258	Phosphorylation	DVPGGGPSSSAPAGA CCCCCCCCCCCCCCC	39.95	23663014
259	Phosphorylation	VPGGGPSSSAPAGAE CCCCCCCCCCCCCCC	33.38	23663014
260	Phosphorylation	PGGGPSSSAPAGAEA CCCCCCCCCCCCCCC	42.48	23663014
273	Phosphorylation	EADGPKASPEARPQI CCCCCCCCCCCCCCC	29.42	30266825
282	Phosphorylation	EARPQIPTKPRVPGK CCCCCCCCCCCCCCC	56.14	30576142
289	Acetylation	TKPRVPGKLQELASP CCCCCCCCHHHHCCC	40.71	25953088
295	Phosphorylation	GKLQELASPPAGRPT CCHHHHCCCCCCCCC	44.00	19664994
302	Phosphorylation	SPPAGRPTPAPRKAS CCCCCCCCCCCCCCC	30.62	26055452
309	Phosphorylation	TPAPRKASESTTPAP CCCCCCCCCCCCCCC	34.81	30266825
311	Phosphorylation	APRKASESTTPAPPT CCCCCCCCCCCCCCC	34.78	30266825
312	Phosphorylation	PRKASESTTPAPPTP CCCCCCCCCCCCCCC	32.52	30266825
313	Phosphorylation	RKASESTTPAPPTPR CCCCCCCCCCCCCCC	27.08	29255136
318	Phosphorylation	STTPAPPTPRPRSSL CCCCCCCCCCCCCHH	30.76	29255136
323	Phosphorylation	PPTPRPRSSLQQENL CCCCCCCCHHHHHCH	37.78	22617229
324	Phosphorylation	PTPRPRSSLQQENLV CCCCCCCHHHHHCHH	31.32	21712546
338	Phosphorylation	VEQAGSSSLVNGRLH HHHHCCCHHCCCEEC	37.73	28555341
355	Phosphorylation	PVPKPRGTPKPSEGT CCCCCCCCCCCCCCC	29.44	22496350
359	Phosphorylation	PRGTPKPSEGTPAPR CCCCCCCCCCCCCCC	55.58	28857561
362	Phosphorylation	TPKPSEGTPAPRKDP CCCCCCCCCCCCCCC	16.54	22496350
367	Acetylation	EGTPAPRKDPPWITL CCCCCCCCCCCCEEE	73.80	7670103
373	Phosphorylation	RKDPPWITLVQAEPK CCCCCCEEEEECCCC	19.60	26074081
389	Phosphorylation	KPAPLPPSSSPGPPS CCCCCCCCCCCCCCC	41.32	30266825
390	Phosphorylation	PAPLPPSSSPGPPSQ CCCCCCCCCCCCCCC	46.45	30266825
391	Phosphorylation	APLPPSSSPGPPSQD CCCCCCCCCCCCCCC	37.91	30266825
396	Phosphorylation	SSSPGPPSQDSRQVE CCCCCCCCCCCCCHH	50.15	30266825
399	Phosphorylation	PGPPSQDSRQVENGG CCCCCCCCCCHHCCC	19.58	23403867
407	Phosphorylation	RQVENGGTEEVAQPS CCHHCCCCCCCCCCC	30.30	30266825
414	Phosphorylation	TEEVAQPSPTASLES CCCCCCCCCCCCCCC	23.85	30266825
416	Phosphorylation	EVAQPSPTASLESKP CCCCCCCCCCCCCCC	32.96	30266825
418	Phosphorylation	AQPSPTASLESKPYN CCCCCCCCCCCCCCC	34.84	30266825
421	Phosphorylation	SPTASLESKPYNPFE CCCCCCCCCCCCCCC	44.20	30266825
424	Phosphorylation	ASLESKPYNPFEEEE CCCCCCCCCCCCHHH	38.92	25247763
443	Phosphorylation	EEAPAAPSLATSPAL HCCCCCCCCCCCCCC	26.38	23927012
446	Phosphorylation	PAAPSLATSPALGHP CCCCCCCCCCCCCCC	40.30	29978859
447	Phosphorylation	AAPSLATSPALGHPE CCCCCCCCCCCCCCC	11.54	21815630
455	Phosphorylation	PALGHPESTPKSLHP CCCCCCCCCCCCCCC	55.23	25627689
456	Phosphorylation	ALGHPESTPKSLHPW CCCCCCCCCCCCCCC	32.83	21815630
459	Phosphorylation	HPESTPKSLHPWYGI CCCCCCCCCCCCCCC	32.87	22167270
464	Phosphorylation	PKSLHPWYGITPTSS CCCCCCCCCCCCCCC	11.78	22167270
467	Phosphorylation	LHPWYGITPTSSPKT CCCCCCCCCCCCCCC	19.02	22167270
469	Phosphorylation	PWYGITPTSSPKTKK CCCCCCCCCCCCCCC	32.94	22167270
470	Phosphorylation	WYGITPTSSPKTKKR CCCCCCCCCCCCCCC	45.93	22167270
471	Phosphorylation	YGITPTSSPKTKKRP CCCCCCCCCCCCCCC	32.66	22167270
474	Phosphorylation	TPTSSPKTKKRPAPR CCCCCCCCCCCCCCC	45.22	23403867
484	Phosphorylation	RPAPRAPSASPLALH CCCCCCCCCCHHHHH	40.15	29255136
486	Phosphorylation	APRAPSASPLALHAS CCCCCCCCHHHHHHH	25.45	29255136
493	Phosphorylation	SPLALHASRLSHSEP CHHHHHHHHCCCCCC	24.01	23927012
496	Phosphorylation	ALHASRLSHSEPPSA HHHHHHCCCCCCCCC	24.69	23927012
498	Phosphorylation	HASRLSHSEPPSATP HHHHCCCCCCCCCCC	48.71	19690332
502	Phosphorylation	LSHSEPPSATPSPAL CCCCCCCCCCCCCCC	56.33	23927012
504	Phosphorylation	HSEPPSATPSPALSV CCCCCCCCCCCCCCH	28.74	23927012
506	Phosphorylation	EPPSATPSPALSVES CCCCCCCCCCCCHHH	20.60	23927012
510	Phosphorylation	ATPSPALSVESLSSE CCCCCCCCHHHCCCC	26.41	23927012
513	Phosphorylation	SPALSVESLSSESAS CCCCCHHHCCCCCCC	31.25	23927012
515	Phosphorylation	ALSVESLSSESASQT CCCHHHCCCCCCCCC	41.14	23927012
516	Phosphorylation	LSVESLSSESASQTA CCHHHCCCCCCCCCC	40.64	23927012
518	Phosphorylation	VESLSSESASQTAGA HHHCCCCCCCCCCCC	34.86	25137130
520	Phosphorylation	SLSSESASQTAGAEL HCCCCCCCCCCCCHH	37.03	25137130
522	Phosphorylation	SSESASQTAGAELLE CCCCCCCCCCCHHHC	25.27	25137130
536	Phosphorylation	EPPAVPKSSSEPAVH CCCCCCCCCCCCCCC	32.78	30576142
537	Phosphorylation	PPAVPKSSSEPAVHA CCCCCCCCCCCCCCC	44.86	28450419
538	Phosphorylation	PAVPKSSSEPAVHAP CCCCCCCCCCCCCCC	55.48	28450419
547	Phosphorylation	PAVHAPGTPGNPVSL CCCCCCCCCCCCCCC	27.28	25159151
553	Phosphorylation	GTPGNPVSLSTNSSL CCCCCCCCCCCCCCC	20.19	28450419
555	Phosphorylation	PGNPVSLSTNSSLAS CCCCCCCCCCCCCCC	20.31	28450419
556	Phosphorylation	GNPVSLSTNSSLASS CCCCCCCCCCCCCCC	44.40	28450419
558	Phosphorylation	PVSLSTNSSLASSGE CCCCCCCCCCCCCCC	27.15	28450419
559	Phosphorylation	VSLSTNSSLASSGEL CCCCCCCCCCCCCCC	29.99	28450419
562	Phosphorylation	STNSSLASSGELVEP CCCCCCCCCCCCCCC	44.14	28450419
563	Phosphorylation	TNSSLASSGELVEPR CCCCCCCCCCCCCCH	30.58	23927012
578	Phosphorylation	VEQMPQASPGLAPRT HHHCCCCCCCCCCCC	17.79	19664994
585	Phosphorylation	SPGLAPRTRGSSGPQ CCCCCCCCCCCCCCC	38.02	28102081
588	Phosphorylation	LAPRTRGSSGPQPAK CCCCCCCCCCCCCCC	28.91	22199227
589	Phosphorylation	APRTRGSSGPQPAKP CCCCCCCCCCCCCCC	58.26	25394399
598	Phosphorylation	PQPAKPCSGATPTPL CCCCCCCCCCCCCCE	39.73	22199227
601	Phosphorylation	AKPCSGATPTPLLLV CCCCCCCCCCCEEEE	31.08	22199227
603	Phosphorylation	PCSGATPTPLLLVGD CCCCCCCCCEEEECC	23.39	30576142
612	Phosphorylation	LLLVGDRSPVPSPGS EEEECCCCCCCCCCC	34.68	23401153
616	Phosphorylation	GDRSPVPSPGSSSPQ CCCCCCCCCCCCCCC	41.97	23401153
616	Ubiquitination	GDRSPVPSPGSSSPQ CCCCCCCCCCCCCCC	41.97	23000965
619	Phosphorylation	SPVPSPGSSSPQLQV CCCCCCCCCCCCEEC	30.90	22167270
619	Ubiquitination	SPVPSPGSSSPQLQV CCCCCCCCCCCCEEC	30.90	23000965
620	Phosphorylation	PVPSPGSSSPQLQVK CCCCCCCCCCCEECC	52.53	22167270
621	Phosphorylation	VPSPGSSSPQLQVKS CCCCCCCCCCEECCC	20.54	19664994
622	Ubiquitination	PSPGSSSPQLQVKSS CCCCCCCCCEECCCC	40.63	23000965
627	Ubiquitination	SSPQLQVKSSCKENP CCCCEECCCCCCCCC	24.56	23000965
628	Phosphorylation	SPQLQVKSSCKENPF CCCEECCCCCCCCCC	41.95	23312004
629	Phosphorylation	PQLQVKSSCKENPFN CCEECCCCCCCCCCC	24.40	21712546
640	Phosphorylation	NPFNRKPSPAASPAT CCCCCCCCCCCCCCC	30.15	29255136
644	Phosphorylation	RKPSPAASPATKKAT CCCCCCCCCCCCCCC	19.71	22167270
647	Phosphorylation	SPAASPATKKATKGS CCCCCCCCCCCCCCC	36.14	23927012
655	Malonylation	KKATKGSKPVRPPAP CCCCCCCCCCCCCCC	57.09	32601280
670	Methylation	GHGFPLIKRKVQADQ CCCCCCEEEECCCCC	54.58	115973051
678	Phosphorylation	RKVQADQYIPEEDIH EECCCCCCCCHHHCC	20.99	27642862
708	Ubiquitination	RGVLLEEKLRGGLNE CCHHHHHHHCCCCCC	33.82	29967540
740	Phosphorylation	HLLVRRESELIYVFK HHHHCCHHHHEEEEE	35.57	28450419
744	Phosphorylation	RRESELIYVFKQQNL CCHHHHEEEEEHHCH	16.68	27642862
760	Phosphorylation	QRQADVEYELRCLLN HHHCHHHHHHHHHHC	21.80	28102081
768	Ubiquitination	ELRCLLNKPEKDWTE HHHHHHCCCCCCCCH	55.30	29967540
771	Ubiquitination	CLLNKPEKDWTEEDR HHHCCCCCCCCHHHH	68.07	29967540
782	Ubiquitination	EEDRAREKVLMQELV HHHHHHHHHHHHHHH	34.81	29967540
822	Ubiquitination	MLEAMIKKKEFQREA HHHHHHHHHHHHHHC	46.76	23000965
825	Ubiquitination	AMIKKKEFQREAEPE HHHHHHHHHHHCCCC	13.09	23000965
828	Ubiquitination	KKKEFQREAEPEGKK HHHHHHHHCCCCCCC	46.75	23000965
833	Ubiquitination	QREAEPEGKKKGKFK HHHCCCCCCCCCHHH	58.77	23000965
840	Ubiquitination	GKKKGKFKTMKMLKL CCCCCHHHHHHHHHH	51.71	23000965
841	Phosphorylation	KKKGKFKTMKMLKLL CCCCHHHHHHHHHHH	25.75	26657352
843	Ubiquitination	KGKFKTMKMLKLLGN CCHHHHHHHHHHHCC	46.44	23000965
846	Ubiquitination	FKTMKMLKLLGNKRD HHHHHHHHHHCCCCC	37.86	23000965
847	Ubiquitination	KTMKMLKLLGNKRDA HHHHHHHHHCCCCCC	6.97	23000965
849	Ubiquitination	MKMLKLLGNKRDAKS HHHHHHHCCCCCCCC	48.31	23000965
850	Ubiquitination	KMLKLLGNKRDAKSK HHHHHHCCCCCCCCC	35.84	23000965
851	Ubiquitination	MLKLLGNKRDAKSKS HHHHHCCCCCCCCCC	50.27	23000965
852	Ubiquitination	LKLLGNKRDAKSKSP HHHHCCCCCCCCCCC	53.79	23000965
853	Ubiquitination	KLLGNKRDAKSKSPR HHHCCCCCCCCCCCC	61.02	23000965
855	Ubiquitination	LGNKRDAKSKSPRDK HCCCCCCCCCCCCCC	63.81	23000965
858	Ubiquitination	KRDAKSKSPRDKS-- CCCCCCCCCCCCC--	32.05	23000965
860	Ubiquitination	DAKSKSPRDKS---- CCCCCCCCCCC----	71.70	23000965
897	Ubiquitination	----------------------------------------- -----------------------------------------		23000965
900	Ubiquitination	-------------------------------------------- --------------------------------------------		23000965
903	Ubiquitination	----------------------------------------------- -----------------------------------------------		23000965
904	Ubiquitination	------------------------------------------------ ------------------------------------------------		23000965
906	Ubiquitination	-------------------------------------------------- --------------------------------------------------		23000965
907	Ubiquitination	--------------------------------------------------- ---------------------------------------------------		23000965
908	Ubiquitination	---------------------------------------------------- ----------------------------------------------------		23000965
909	Ubiquitination	----------------------------------------------------- -----------------------------------------------------		23000965
910	Ubiquitination	------------------------------------------------------ ------------------------------------------------------		23000965
912	Ubiquitination	-------------------------------------------------------- --------------------------------------------------------		23000965
915	Ubiquitination	----------------------------------------------------------- -----------------------------------------------------------		23000965
917	Ubiquitination	------------------------------------------------------------- -------------------------------------------------------------		23000965

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of MILK1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of MILK1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MILK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EHD1_HUMAN	EHD1	physical	22456504
KI13B_HUMAN	KIF13B	physical	27173435
CING_HUMAN	CGN	physical	27173435
ZBT21_HUMAN	ZBTB21	physical	27173435
CBY1_HUMAN	CBY1	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MILK1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-467; SER-471; SER-484;SER-486 AND SER-578, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-484; SER-486; SER-578AND SER-621, AND MASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295; SER-309; THR-313;THR-318; THR-467; SER-470; SER-471; SER-484; SER-486 AND SER-578, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-578, AND MASSSPECTROMETRY.	15302935 [Abstract]