dbPTM

MARK4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MARK4_HUMAN
UniProt AC	Q96L34
Protein Name	MAP/microtubule affinity-regulating kinase 4
Gene Name	MARK4
Organism	Homo sapiens (Human).
Sequence Length	752
Subcellular Localization	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, microtubule organizing center . Cytoplasm, cytoskeleton, cilium basal body . Cytoplasm, cytoskeleton, cilium axoneme . Cytoplasm . Cell projection, dendrite . No
Protein Description	Serine/threonine-protein kinase. [PubMed: 15009667]
Protein Sequence	MSSRTVLAPGNDRNSDTHGTLGSGRSSDKGPSWSSRSLGARCRNSIASCPEEQPHVGNYRLLRTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPSSLQKLFREVRIMKGLNHPNIVKLFEVIETEKTLYLVMEYASAGEVFDYLVSHGRMKEKEARAKFRQIVSAVHYCHQKNIVHRDLKAENLLLDAEANIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDIWSLGVILYTLVSGSLPFDGHNLKELRERVLRGKYRVPFYMSTDCESILRRFLVLNPAKRCTLEQIMKDKWINIGYEGEELKPYTEPEEDFGDTKRIEVMVGMGYTREEIKESLTSQKYNEVTATYLLLGRKTEEGGDRGAPGLALARVRAPSDTTNGTSSSKGTSHSKGQRSSSSTYHRQRRHSDFCGPSPAPLHPKRSPTSTGEAELKEERLPGRKASCSTAGSGSRGLPPSSPMVSSAHNPNKAEIPERRKDSTSTPNNLPPSMMTRRNTYVCTERPGAERPSLLPNGKENSSGTPRVPPASPSSHSLAPPSGERSRLARGSTIRSTFHGGQVRDRRAGGGGGGGVQNGPPASPTLAHEAAPLPAGRPRPTTNLFTKLTSKLTRRVADEPERIGGPEVTSCHLPWDQTETAPRLLRFPWSVKLTSSRPPEALMAALRQATAAARCRCRQPQPFLLACLHGGAGGPEPLSHFEVEVCQLPRPGLRGVLFRRVAGTALAFRTLVTRISNDLEL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSSRTVLAP ------CCCCEEECC	28.64	19413330
2	Phosphorylation	------MSSRTVLAP ------CCCCEEECC	28.64	19369195
2 (in isoform 2)	Acetylation	-	28.64	-
15	Phosphorylation	APGNDRNSDTHGTLG CCCCCCCCCCCCCCC	44.79	22210691
17	Phosphorylation	GNDRNSDTHGTLGSG CCCCCCCCCCCCCCC	23.25	22210691
20	Phosphorylation	RNSDTHGTLGSGRSS CCCCCCCCCCCCCCC	21.97	23186163
23	Phosphorylation	DTHGTLGSGRSSDKG CCCCCCCCCCCCCCC	33.86	20071362
26	Phosphorylation	GTLGSGRSSDKGPSW CCCCCCCCCCCCCCC	46.55	17192257
26 (in isoform 2)	Phosphorylation	-	46.55	-
27	Phosphorylation	TLGSGRSSDKGPSWS CCCCCCCCCCCCCCC	41.82	22617229
29	Ubiquitination	GSGRSSDKGPSWSSR CCCCCCCCCCCCCHH	74.74	29967540
32	Phosphorylation	RSSDKGPSWSSRSLG CCCCCCCCCCHHHHH	48.54	27794612
34	Phosphorylation	SDKGPSWSSRSLGAR CCCCCCCCHHHHHHH	21.88	27794612
35	Ubiquitination	DKGPSWSSRSLGARC CCCCCCCHHHHHHHH	21.45	27667366
35	Phosphorylation	DKGPSWSSRSLGARC CCCCCCCHHHHHHHH	21.45	27794612
45	Phosphorylation	LGARCRNSIASCPEE HHHHHHHCHHCCCCC	10.53	29978859
48	Phosphorylation	RCRNSIASCPEEQPH HHHHCHHCCCCCCCC	28.65	29978859
60	Methylation	QPHVGNYRLLRTIGK CCCCCCCHHHEEECC	31.11	54557239
64	Phosphorylation	GNYRLLRTIGKGNFA CCCHHHEEECCCCCH	33.77	28152594
67	Ubiquitination	RLLRTIGKGNFAKVK HHHEEECCCCCHHHH	46.99	-
88	Ubiquitination	TGREVAIKIIDKTQL CCCHHHEEECCCCCC	24.70	-
92	Ubiquitination	VAIKIIDKTQLNPSS HHEEECCCCCCCHHH	27.97	29967540
93	Phosphorylation	AIKIIDKTQLNPSSL HEEECCCCCCCHHHH	34.24	30622161
98	Phosphorylation	DKTQLNPSSLQKLFR CCCCCCHHHHHHHHH	42.48	30622161
99	Phosphorylation	KTQLNPSSLQKLFRE CCCCCHHHHHHHHHH	35.76	30622161
102	Ubiquitination	LNPSSLQKLFREVRI CCHHHHHHHHHHHHH	55.69	27667366
111	Ubiquitination	FREVRIMKGLNHPNI HHHHHHHCCCCCCCH	58.85	29967540
116	Ubiquitination	IMKGLNHPNIVKLFE HHCCCCCCCHHHHEE	31.04	22817900
183	Ubiquitination	NIVHRDLKAENLLLD CCCCCCCHHHHHEEE	58.92	22817900
183 (in isoform 1)	Ubiquitination	-	58.92	21906983
183 (in isoform 2)	Ubiquitination	-	58.92	21906983
210	Phosphorylation	SNEFTLGSKLDTFCG CCCEECCCCHHHCCC	32.91	28464451
214 (in isoform 2)	Phosphorylation	-	31.12	-
214	Phosphorylation	TLGSKLDTFCGSPPY ECCCCHHHCCCCCCC	31.12	14976552
218 (in isoform 2)	Phosphorylation	-	24.71	-
218	Phosphorylation	KLDTFCGSPPYAAPE CHHHCCCCCCCCCHH	24.71	19664994
221	Phosphorylation	TFCGSPPYAAPELFQ HCCCCCCCCCHHHHC	20.84	23898821
230	Ubiquitination	APELFQGKKYDGPEV CHHHHCCCCCCCCCC	37.06	27667366
231	Ubiquitination	PELFQGKKYDGPEVD HHHHCCCCCCCCCCC	56.23	-
297	Ubiquitination	FLVLNPAKRCTLEQI HHHCCHHHCCCHHHH	49.62	27667366
306	Ubiquitination	CTLEQIMKDKWINIG CCHHHHHHCCCEECC	58.76	29967540
320	Ubiquitination	GYEGEELKPYTEPEE CCCCCCCCCCCCCHH	38.98	29967540
351	Phosphorylation	TREEIKESLTSQKYN CHHHHHHHHHHCHHH	31.76	23403867
353	Phosphorylation	EEIKESLTSQKYNEV HHHHHHHHHCHHHHH	38.09	29496907
356	Ubiquitination	KESLTSQKYNEVTAT HHHHHHCHHHHHHHH	50.22	-
364	Phosphorylation	YNEVTATYLLLGRKT HHHHHHHHHHHCCCC	7.90	29496907
391	Phosphorylation	LARVRAPSDTTNGTS EEEEECCCCCCCCCC	46.75	21712546
393	Phosphorylation	RVRAPSDTTNGTSSS EEECCCCCCCCCCCC	26.88	30576142
394	Phosphorylation	VRAPSDTTNGTSSSK EECCCCCCCCCCCCC	36.32	30576142
398	Phosphorylation	SDTTNGTSSSKGTSH CCCCCCCCCCCCCCC	33.73	28985074
400	Phosphorylation	TTNGTSSSKGTSHSK CCCCCCCCCCCCCCC	34.57	28985074
403	Phosphorylation	GTSSSKGTSHSKGQR CCCCCCCCCCCCCCC	27.31	30576142
404	Phosphorylation	TSSSKGTSHSKGQRS CCCCCCCCCCCCCCC	34.14	21712546
423	Phosphorylation	YHRQRRHSDFCGPSP HCHHHCCCCCCCCCC	30.48	27273156
429	Phosphorylation	HSDFCGPSPAPLHPK CCCCCCCCCCCCCCC	20.98	23927012
438	Phosphorylation	APLHPKRSPTSTGEA CCCCCCCCCCCCCCH	38.36	29255136
440	Phosphorylation	LHPKRSPTSTGEAEL CCCCCCCCCCCCHHH	39.92	29255136
441	Phosphorylation	HPKRSPTSTGEAELK CCCCCCCCCCCHHHH	37.49	29255136
442	Phosphorylation	PKRSPTSTGEAELKE CCCCCCCCCCHHHHH	41.33	29255136
458	Phosphorylation	RLPGRKASCSTAGSG CCCCCCCCCCCCCCC	16.19	24719451
460	Phosphorylation	PGRKASCSTAGSGSR CCCCCCCCCCCCCCC	20.85	23403867
461	Phosphorylation	GRKASCSTAGSGSRG CCCCCCCCCCCCCCC	39.09	23403867
464	Phosphorylation	ASCSTAGSGSRGLPP CCCCCCCCCCCCCCC	31.00	23403867
466	Phosphorylation	CSTAGSGSRGLPPSS CCCCCCCCCCCCCCC	25.52	23403867
472	Phosphorylation	GSRGLPPSSPMVSSA CCCCCCCCCCCCCCC	45.17	28348404
473	Phosphorylation	SRGLPPSSPMVSSAH CCCCCCCCCCCCCCC	23.84	28348404
478	Phosphorylation	PSSPMVSSAHNPNKA CCCCCCCCCCCCCCC	23.33	25022875
494	Phosphorylation	IPERRKDSTSTPNNL CCHHHCCCCCCCCCC	27.56	22210691
495	Phosphorylation	PERRKDSTSTPNNLP CHHHCCCCCCCCCCC	46.91	22210691
496	Phosphorylation	ERRKDSTSTPNNLPP HHHCCCCCCCCCCCH	45.91	30576142
496 (in isoform 2)	Phosphorylation	-	45.91	-
497	Phosphorylation	RRKDSTSTPNNLPPS HHCCCCCCCCCCCHH	30.08	30576142
504 (in isoform 2)	Phosphorylation	-	21.17	-
504	Phosphorylation	TPNNLPPSMMTRRNT CCCCCCHHHHCCCCE	21.17	29759185
507 (in isoform 2)	Phosphorylation	-	20.17	-
507	Phosphorylation	NLPPSMMTRRNTYVC CCCHHHHCCCCEEEE	20.17	19651622
511	Phosphorylation	SMMTRRNTYVCTERP HHHCCCCEEEEECCC	18.60	30576142
512	Phosphorylation	MMTRRNTYVCTERPG HHCCCCEEEEECCCC	9.33	23312004
543	Phosphorylation	TPRVPPASPSSHSLA CCCCCCCCCCCCCCC	30.96	14976552
545	Phosphorylation	RVPPASPSSHSLAPP CCCCCCCCCCCCCCC	37.88	23403867
546	Phosphorylation	VPPASPSSHSLAPPS CCCCCCCCCCCCCCC	22.08	23403867
548	Phosphorylation	PASPSSHSLAPPSGE CCCCCCCCCCCCCCC	28.33	23186163
553	Phosphorylation	SHSLAPPSGERSRLA CCCCCCCCCCCHHHC	52.83	23186163
561	Methylation	GERSRLARGSTIRST CCCHHHCCCCCCCCE	44.16	115482595
563	Phosphorylation	RSRLARGSTIRSTFH CHHHCCCCCCCCEEC	18.33	24719451
564	Phosphorylation	SRLARGSTIRSTFHG HHHCCCCCCCCEECC	24.19	-
567	Phosphorylation	ARGSTIRSTFHGGQV CCCCCCCCEECCCEE	31.41	23312004
568	Phosphorylation	RGSTIRSTFHGGQVR CCCCCCCEECCCEEE	15.14	23312004
594	Phosphorylation	VQNGPPASPTLAHEA CCCCCCCCCCCCCCC	24.96	26657352
596	Phosphorylation	NGPPASPTLAHEAAP CCCCCCCCCCCCCCC	33.80	28152594
612	Phosphorylation	PAGRPRPTTNLFTKL CCCCCCCCCCHHHHH	29.60	28152594
613	Phosphorylation	AGRPRPTTNLFTKLT CCCCCCCCCHHHHHH	32.38	28152594
617	Phosphorylation	RPTTNLFTKLTSKLT CCCCCHHHHHHHHHH	28.45	28152594
622	Ubiquitination	LFTKLTSKLTRRVAD HHHHHHHHHHHHHCC	48.95	-
628 (in isoform 2)	Phosphorylation	-	17.40	26437602
650 (in isoform 2)	Phosphorylation	-	37.02	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
214	T	Phosphorylation	Kinase	STK11	Q15831	PhosphoELM

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
29	K	Phosphorylation	14976552
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation.
29	K	ubiquitylation	14976552
Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation.
214	T	Phosphorylation	14976552
Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MARK4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MYH9_HUMAN	MYH9	physical	14594945
MYH10_HUMAN	MYH10	physical	14594945
HSP74_HUMAN	HSPA4	physical	14594945
TBG1_HUMAN	TUBG1	physical	14594945
ACTS_HUMAN	ACTA1	physical	14594945
TBB5_HUMAN	TUBB	physical	14594945
TBA1A_HUMAN	TUBA1A	physical	14594945
MTAP2_HUMAN	MAP2	physical	14594945
TAU_HUMAN	MAPT	physical	14594945
MAP4_HUMAN	MAP4	physical	14594945
USP9X_HUMAN	USP9X	physical	18254724
ARHG2_HUMAN	ARHGEF2	physical	14676191
MTCL1_HUMAN	MTCL1	physical	14676191
KCD20_HUMAN	KCTD20	physical	14676191
2AAA_HUMAN	PPP2R1A	physical	14676191
PNMA1_HUMAN	PNMA1	physical	14676191
USP9X_HUMAN	USP9X	physical	14676191
SOGA1_HUMAN	SOGA1	physical	14676191
RNF41_HUMAN	RNF41	physical	14676191
MY18A_HUMAN	MYO18A	physical	14676191
1433F_HUMAN	YWHAH	physical	14676191
MBB1A_HUMAN	MYBBP1A	physical	14676191
PP2AB_HUMAN	PPP2CB	physical	14676191
SMCA4_HUMAN	SMARCA4	physical	14676191
TBG1_HUMAN	TUBG1	physical	14676191
CDC42_HUMAN	CDC42	physical	14676191
PNMA2_HUMAN	PNMA2	physical	14676191
KPCI_HUMAN	PRKCI	physical	14676191
DOCK7_HUMAN	DOCK7	physical	14676191
UBP7_HUMAN	USP7	physical	14676191
CYFP1_HUMAN	CYFIP1	physical	14676191
CYFP2_HUMAN	CYFIP2	physical	14676191
RPTOR_HUMAN	RPTOR	physical	23184942
TAU_HUMAN	MAPT	physical	23666762
ODFP2_HUMAN	ODF2	physical	23400999
K1C40_HUMAN	KRT40	physical	25416956
NT2NL_HUMAN	NOTCH2NL	physical	25416956
RPIA_HUMAN	RPIA	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MARK4_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2; THR-17; SER-23;SER-26; SER-423; SER-438 AND SER-545, AND MASS SPECTROMETRY.	19369195 [Abstract]
"Proteomics analysis of protein kinases by target class-selectiveprefractionation and tandem mass spectrometry."; Wissing J., Jaensch L., Nimtz M., Dieterich G., Hornberger R.,Keri G., Wehland J., Daub H.; Mol. Cell. Proteomics 6:537-547(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND THR-214, AND MASSSPECTROMETRY.	17192257 [Abstract]
"LKB1 is a master kinase that activates 13 kinases of the AMPKsubfamily, including MARK/PAR-1."; Lizcano J.M., Goeransson O., Toth R., Deak M., Morrice N.A.,Boudeau J., Hawley S.A., Udd L., Maekelae T.P., Hardie D.G.,Alessi D.R.; EMBO J. 23:833-843(2004). Cited for: ENZYME REGULATION, PHOSPHORYLATION AT THR-214, AND MUTAGENESIS OFTHR-214.	14976552 [Abstract]

show

show

show