dbPTM

EHBP1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	EHBP1_HUMAN
UniProt AC	Q8NDI1
Protein Name	EH domain-binding protein 1
Gene Name	EHBP1
Organism	Homo sapiens (Human).
Sequence Length	1231
Subcellular Localization	Cytoplasm . Membrane . Endosome . Mostly found in cytosol and plasma membrane.
Protein Description	May play a role in actin reorganization. Links clathrin-mediated endocytosis to the actin cytoskeleton. May act as Rab effector protein and play a role in vesicle trafficking. [PubMed: 14676205]
Protein Sequence	MASVWKRLQRVGKHASKFQFVASYQELMVECTKKWQPDKLVVVWTRRSRRKSSKAHSWQPGIKNPYRGVVVWPVPENIEITVTLFKDPHAEEFEDKEWTFVIENESPSGRRKALATSSINMKQYASPMPTQTDVKLKFKPLSKKVVSAALQFSLSCIFLREGKATDEDMQSLASLMSMKQADIGNLDDFEEDNEDDDENRVNQEEKAAKITEIVNQLNALSSLDEDQDDCIKQANMRSAKSASSSEELINKLNFLDEAEKDLATVNSNPFDDPDAAELNPFGDPDSEEPITETASPRKTEDSFYNNSYNPFKEVQTPQYLNPFDEPEAFVTIKDSPPQSTKRKNIRPVDMSKYLYADSSKTEEEELDESNPFYEPKSTPPPNNLVNPVQELETERRVKRKAPAPPVLSPKTGVLNENTVSAGKDLSTSPKPSPIPSPVLGRKPNASQSLLVWCKEVTKNYRGVKITNFTTSWRNGLSFCAILHHFRPDLIDYKSLNPQDIKENNKKAYDGFASIGISRLLEPSDMVLLAIPDKLTVMTYLYQIRAHFSGQELNVVQIEENSSKSTYKVGNYETDTNSSVDQEKFYAELSDLKREPELQQPISGAVDFLSQDDSVFVNDSGVGESESEHQTPDDHLSPSTASPYCRRTKSDTEPQKSQQSSGRTSGSDDPGICSNTDSTQAQVLLGKKRLLKAETLELSDLYVSDKKKDMSPPFICEETDEQKLQTLDIGSNLEKEKLENSRSLECRSDPESPIKKTSLSPTSKLGYSYSRDLDLAKKKHASLRQTESDPDADRTTLNHADHSSKIVQHRLLSRQEELKERARVLLEQARRDAALKAGNKHNTNTATPFCNRQLSDQQDEERRRQLRERARQLIAEARSGVKMSELPSYGEMAAEKLKERSKASGDENDNIEIDTNEEIPEGFVVGGGDELTNLENDLDTPEQNSKLVDLKLKKLLEVQPQVANSPSSAAQKAVTESSEQDMKSGTEDLRTERLQKTTERFRNPVVFSKDSTVRKTQLQSFSQYIENRPEMKRQRSIQEDTKKGNEEKAAITETQRKPSEDEVLNKGFKDTSQYVVGELAALENEQKQIDTRAALVEKRLRYLMDTGRNTEEEEAMMQEWFMLVNKKNALIRRMNQLSLLEKEHDLERRYELLNRELRAMLAIEDWQKTEAQKRREQLLLDELVALVNKRDALVRDLDAQEKQAEEEDEHLERTLEQNKGKMAKKEEKCVLQ

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
33	Acetylation	ELMVECTKKWQPDKL HHHHHHHHHCCCCCE	64.77	19667021
34	Acetylation	LMVECTKKWQPDKLV HHHHHHHHCCCCCEE	32.67	19667031
106	Phosphorylation	TFVIENESPSGRRKA EEEEECCCCCCCCCE	35.63	28348404
124	Phosphorylation	SSINMKQYASPMPTQ CCCCHHHHCCCCCCC	11.97	22210691
126	Phosphorylation	INMKQYASPMPTQTD CCHHHHCCCCCCCCC	19.46	22210691
130	Phosphorylation	QYASPMPTQTDVKLK HHCCCCCCCCCEEEE	37.54	22210691
132	Phosphorylation	ASPMPTQTDVKLKFK CCCCCCCCCEEEEEC	45.28	22210691
163	Ubiquitination	CIFLREGKATDEDMQ HHHHCCCCCCHHHHH	43.28	21906983
163 (in isoform 1)	Ubiquitination	-	43.28	21890473
163 (in isoform 2)	Ubiquitination	-	43.28	21890473
163 (in isoform 3)	Ubiquitination	-	43.28	21890473
165	Phosphorylation	FLREGKATDEDMQSL HHCCCCCCHHHHHHH	43.33	23927012
171	Phosphorylation	ATDEDMQSLASLMSM CCHHHHHHHHHHHHH	21.18	29255136
171 (in isoform 2)	Phosphorylation	-	21.18	21406692
174	Phosphorylation	EDMQSLASLMSMKQA HHHHHHHHHHHHHHH	29.87	29255136
174 (in isoform 2)	Phosphorylation	-	29.87	21406692
177	Phosphorylation	QSLASLMSMKQADIG HHHHHHHHHHHHCCC	28.28	29255136
206	Ubiquitination	NRVNQEEKAAKITEI CCCCHHHHHHHHHHH	53.55	-
222	Phosphorylation	NQLNALSSLDEDQDD HHHHHHHCCCCCHHH	40.46	-
238	Phosphorylation	IKQANMRSAKSASSS HHHHHHHHHCCCCCH	29.31	24905233
241	Phosphorylation	ANMRSAKSASSSEEL HHHHHHCCCCCHHHH	32.85	24905233
243	Phosphorylation	MRSAKSASSSEELIN HHHHCCCCCHHHHHH	41.30	24905233
244	Phosphorylation	RSAKSASSSEELINK HHHCCCCCHHHHHHH	41.29	24905233
245	Phosphorylation	SAKSASSSEELINKL HHCCCCCHHHHHHHH	32.37	25307156
264	Phosphorylation	EAEKDLATVNSNPFD HHHHHHHCCCCCCCC	27.76	26074081
267	Phosphorylation	KDLATVNSNPFDDPD HHHHCCCCCCCCCCC	41.36	26074081
286	Phosphorylation	NPFGDPDSEEPITET CCCCCCCCCCCCCCC	49.61	28450419
291	Phosphorylation	PDSEEPITETASPRK CCCCCCCCCCCCCCC	37.69	28450419
293	Phosphorylation	SEEPITETASPRKTE CCCCCCCCCCCCCCC	24.64	28450419
295	Phosphorylation	EPITETASPRKTEDS CCCCCCCCCCCCCCC	32.43	28387310
299	Phosphorylation	ETASPRKTEDSFYNN CCCCCCCCCCCCCCC	46.43	28796482
302	Phosphorylation	SPRKTEDSFYNNSYN CCCCCCCCCCCCCCC	24.33	25159151
304	Phosphorylation	RKTEDSFYNNSYNPF CCCCCCCCCCCCCCC	19.86	28796482
307	Phosphorylation	EDSFYNNSYNPFKEV CCCCCCCCCCCCCCC	23.73	25159151
308	Phosphorylation	DSFYNNSYNPFKEVQ CCCCCCCCCCCCCCC	29.04	28796482
316	Phosphorylation	NPFKEVQTPQYLNPF CCCCCCCCCCCCCCC	19.96	30266825
319	Phosphorylation	KEVQTPQYLNPFDEP CCCCCCCCCCCCCCC	15.06	30266825
331	Phosphorylation	DEPEAFVTIKDSPPQ CCCCCEEEECCCCCC	18.82	30266825
335	Phosphorylation	AFVTIKDSPPQSTKR CEEEECCCCCCCCCC	33.21	25159151
339	Phosphorylation	IKDSPPQSTKRKNIR ECCCCCCCCCCCCCC	41.49	30266825
340	Phosphorylation	KDSPPQSTKRKNIRP CCCCCCCCCCCCCCC	29.62	30266825
351	Phosphorylation	NIRPVDMSKYLYADS CCCCCCHHHHHCCCC	17.43	28555341
353	Phosphorylation	RPVDMSKYLYADSSK CCCCHHHHHCCCCCC	9.53	28796482
355	Phosphorylation	VDMSKYLYADSSKTE CCHHHHHCCCCCCCC	12.79	26657352
358	Phosphorylation	SKYLYADSSKTEEEE HHHHCCCCCCCCCHH	26.09	26657352
359	Phosphorylation	KYLYADSSKTEEEEL HHHCCCCCCCCCHHH	44.11	21815630
361	Phosphorylation	LYADSSKTEEEELDE HCCCCCCCCCHHHHC	51.05	28348404
369	Phosphorylation	EEEELDESNPFYEPK CCHHHHCCCCCCCCC	49.85	21815630
373	Phosphorylation	LDESNPFYEPKSTPP HHCCCCCCCCCCCCC	32.36	26074081
377	Phosphorylation	NPFYEPKSTPPPNNL CCCCCCCCCCCCCCC	57.79	30266825
378	Phosphorylation	PFYEPKSTPPPNNLV CCCCCCCCCCCCCCC	46.04	30266825
391 (in isoform 2)	Phosphorylation	-	5.73	21406692
392 (in isoform 2)	Phosphorylation	-	48.12	21406692
393	O-linked_Glycosylation	NPVQELETERRVKRK CHHHHHHHHHHHHHC	47.63	30379171
393	Phosphorylation	NPVQELETERRVKRK CHHHHHHHHHHHHHC	47.63	23927012
397 (in isoform 2)	Phosphorylation	-	9.84	21406692
401 (in isoform 2)	Phosphorylation	-	14.98	21406692
408	Phosphorylation	APAPPVLSPKTGVLN CCCCCCCCCCCCCCC	25.28	29255136
411	Phosphorylation	PPVLSPKTGVLNENT CCCCCCCCCCCCCCC	35.59	23927012
418	Phosphorylation	TGVLNENTVSAGKDL CCCCCCCCCCCCCCC	15.06	26434776
420	Phosphorylation	VLNENTVSAGKDLST CCCCCCCCCCCCCCC	29.30	25159151
426	Phosphorylation	VSAGKDLSTSPKPSP CCCCCCCCCCCCCCC	36.65	29255136
427	Phosphorylation	SAGKDLSTSPKPSPI CCCCCCCCCCCCCCC	57.24	29255136
428	Phosphorylation	AGKDLSTSPKPSPIP CCCCCCCCCCCCCCC	27.79	29255136
432	Phosphorylation	LSTSPKPSPIPSPVL CCCCCCCCCCCCCCC	41.40	29255136
436	Phosphorylation	PKPSPIPSPVLGRKP CCCCCCCCCCCCCCC	28.49	29255136
446	Phosphorylation	LGRKPNASQSLLVWC CCCCCCCCCHHHHHH	27.26	28122231
448	Phosphorylation	RKPNASQSLLVWCKE CCCCCCCHHHHHHHH	22.60	28122231
508	Phosphorylation	KENNKKAYDGFASIG HHHCHHHHHHCHHHC	26.02	24719451
571	Phosphorylation	STYKVGNYETDTNSS CEEEECCEECCCCCC	17.80	27642862
573	Phosphorylation	YKVGNYETDTNSSVD EEECCEECCCCCCCC	37.45	27251275
575	Phosphorylation	VGNYETDTNSSVDQE ECCEECCCCCCCCHH	44.41	29978859
577	Phosphorylation	NYETDTNSSVDQEKF CEECCCCCCCCHHHH	33.55	29978859
578	Phosphorylation	YETDTNSSVDQEKFY EECCCCCCCCHHHHH	31.61	25159151
585	Phosphorylation	SVDQEKFYAELSDLK CCCHHHHHHHHHHHC	15.38	25884760
589	Phosphorylation	EKFYAELSDLKREPE HHHHHHHHHHCCCHH	32.19	29978859
592	Ubiquitination	YAELSDLKREPELQQ HHHHHHHCCCHHHCC	60.76	-
636	Phosphorylation	QTPDDHLSPSTASPY CCCCCCCCCCCCCHH	17.24	-
647	Phosphorylation	ASPYCRRTKSDTEPQ CCHHHCCCCCCCCCC	18.62	26699800
649	Phosphorylation	PYCRRTKSDTEPQKS HHHCCCCCCCCCCHH	49.50	28985074
651	Phosphorylation	CRRTKSDTEPQKSQQ HCCCCCCCCCCHHHH	57.57	26699800
656	Phosphorylation	SDTEPQKSQQSSGRT CCCCCCHHHHCCCCC	28.36	23927012
659	Phosphorylation	EPQKSQQSSGRTSGS CCCHHHHCCCCCCCC	27.42	23927012
660	Phosphorylation	PQKSQQSSGRTSGSD CCHHHHCCCCCCCCC	28.14	23927012
663	Phosphorylation	SQQSSGRTSGSDDPG HHHCCCCCCCCCCCC	40.75	25159151
664	Phosphorylation	QQSSGRTSGSDDPGI HHCCCCCCCCCCCCC	34.63	23927012
666	Phosphorylation	SSGRTSGSDDPGICS CCCCCCCCCCCCCCC	38.31	25159151
673	Phosphorylation	SDDPGICSNTDSTQA CCCCCCCCCCCCHHH	40.62	30108239
675	Phosphorylation	DPGICSNTDSTQAQV CCCCCCCCCCHHHHH	18.29	30108239
677	Phosphorylation	GICSNTDSTQAQVLL CCCCCCCCHHHHHHH	22.00	23927012
678	Phosphorylation	ICSNTDSTQAQVLLG CCCCCCCHHHHHHHC	30.63	23927012
694	Phosphorylation	KRLLKAETLELSDLY HHHHCCEEEEHHHHC	30.32	22617229
698	Phosphorylation	KAETLELSDLYVSDK CCEEEEHHHHCCCCC	19.05	22199227
701	Phosphorylation	TLELSDLYVSDKKKD EEEHHHHCCCCCCCC	11.69	29978859
703	Phosphorylation	ELSDLYVSDKKKDMS EHHHHCCCCCCCCCC	30.10	29978859
710	Phosphorylation	SDKKKDMSPPFICEE CCCCCCCCCCCCCCC	39.28	25159151
718	Phosphorylation	PPFICEETDEQKLQT CCCCCCCCCHHHCHH	23.65	-
725	Phosphorylation	TDEQKLQTLDIGSNL CCHHHCHHCCCCCCH	36.51	29255136
728 (in isoform 2)	Ubiquitination	-	7.98	21890473
728 (in isoform 3)	Ubiquitination	-	7.98	21890473
730	Phosphorylation	LQTLDIGSNLEKEKL CHHCCCCCCHHHHHH	38.28	25850435
740	Phosphorylation	EKEKLENSRSLECRS HHHHHHCCCCCCCCC	17.55	23898821
742	Phosphorylation	EKLENSRSLECRSDP HHHHCCCCCCCCCCC	28.48	23403867
747	Phosphorylation	SRSLECRSDPESPIK CCCCCCCCCCCCCCC	70.00	25159151
751	Phosphorylation	ECRSDPESPIKKTSL CCCCCCCCCCCCCCC	37.00	29255136
756	Phosphorylation	PESPIKKTSLSPTSK CCCCCCCCCCCCCCC	29.87	23403867
757	Phosphorylation	ESPIKKTSLSPTSKL CCCCCCCCCCCCCCC	35.41	23403867
759	Phosphorylation	PIKKTSLSPTSKLGY CCCCCCCCCCCCCCC	26.37	25159151
761	Phosphorylation	KKTSLSPTSKLGYSY CCCCCCCCCCCCCCC	34.81	27794612
762	Phosphorylation	KTSLSPTSKLGYSYS CCCCCCCCCCCCCCC	29.30	23403867
763	Ubiquitination	TSLSPTSKLGYSYSR CCCCCCCCCCCCCCC	48.70	21890473
763 (in isoform 1)	Ubiquitination	-	48.70	21890473
766	Phosphorylation	SPTSKLGYSYSRDLD CCCCCCCCCCCCCHH	18.18	27642862
767	Phosphorylation	PTSKLGYSYSRDLDL CCCCCCCCCCCCHHH	18.16	25159151
768	Phosphorylation	TSKLGYSYSRDLDLA CCCCCCCCCCCHHHH	10.15	30576142
769	Phosphorylation	SKLGYSYSRDLDLAK CCCCCCCCCCHHHHH	17.24	27251275
781	Phosphorylation	LAKKKHASLRQTESD HHHHHCHHHHCCCCC	24.65	29514088
785	Phosphorylation	KHASLRQTESDPDAD HCHHHHCCCCCCCCC	30.90	29116813
787	Phosphorylation	ASLRQTESDPDADRT HHHHCCCCCCCCCCC	58.81	25159151
794	Phosphorylation	SDPDADRTTLNHADH CCCCCCCCCCCHHHH	35.86	29514088
795	Phosphorylation	DPDADRTTLNHADHS CCCCCCCCCCHHHHH	26.85	29514088
802	Phosphorylation	TLNHADHSSKIVQHR CCCHHHHHHHHHHHH	33.60	23186163
803	Phosphorylation	LNHADHSSKIVQHRL CCHHHHHHHHHHHHH	24.00	23186163
812	Phosphorylation	IVQHRLLSRQEELKE HHHHHHHHCHHHHHH	36.19	24719451
846	Phosphorylation	KHNTNTATPFCNRQL CCCCCCCCCCCCCCC	18.17	28555341
854	Phosphorylation	PFCNRQLSDQQDEER CCCCCCCCCCCHHHH	25.65	28258704
883	Phosphorylation	ARSGVKMSELPSYGE HHHCCCHHHCCCHHH	30.20	27251275
887	Phosphorylation	VKMSELPSYGEMAAE CCHHHCCCHHHHHHH	59.39	28555341
888	Phosphorylation	KMSELPSYGEMAAEK CHHHCCCHHHHHHHH	18.37	29978859
964	Phosphorylation	VQPQVANSPSSAAQK HCCCCCCCCCHHHHH	19.20	29255136
966	Phosphorylation	PQVANSPSSAAQKAV CCCCCCCCHHHHHHH	32.22	29255136
967	Phosphorylation	QVANSPSSAAQKAVT CCCCCCCHHHHHHHH	30.89	29255136
974	Phosphorylation	SAAQKAVTESSEQDM HHHHHHHHHCHHHHH	34.70	-
976	Phosphorylation	AQKAVTESSEQDMKS HHHHHHHCHHHHHHH	29.79	-
977	Phosphorylation	QKAVTESSEQDMKSG HHHHHHCHHHHHHHC	32.80	-
981	Sulfoxidation	TESSEQDMKSGTEDL HHCHHHHHHHCCHHH	3.58	30846556
1000 (in isoform 2)	Phosphorylation	-	7.82	21406692
1005 (in isoform 2)	Phosphorylation	-	4.77	21406692
1010	Phosphorylation	PVVFSKDSTVRKTQL CEECCCCCCCCHHHH	32.32	28857561
1011	Phosphorylation	VVFSKDSTVRKTQLQ EECCCCCCCCHHHHH	34.17	28857561
1015	Phosphorylation	KDSTVRKTQLQSFSQ CCCCCCHHHHHHHHH	24.77	29978859
1016 (in isoform 2)	Phosphorylation	-	40.31	21406692
1019	Phosphorylation	VRKTQLQSFSQYIEN CCHHHHHHHHHHHHC	35.14	25159151
1021	Phosphorylation	KTQLQSFSQYIENRP HHHHHHHHHHHHCCH	27.59	23186163
1023	Phosphorylation	QLQSFSQYIENRPEM HHHHHHHHHHCCHHH	14.70	29978859
1023 (in isoform 2)	Phosphorylation	-	14.70	21406692
1035	Phosphorylation	PEMKRQRSIQEDTKK HHHHHHHHHHHHHHH	21.93	25159151
1040	Phosphorylation	QRSIQEDTKKGNEEK HHHHHHHHHHCHHHH	33.62	28176443
1051	Phosphorylation	NEEKAAITETQRKPS HHHHHHCHHCCCCCC	28.40	23927012
1053	Phosphorylation	EKAAITETQRKPSED HHHHCHHCCCCCCHH	25.64	23927012
1058	Phosphorylation	TETQRKPSEDEVLNK HHCCCCCCHHHHHHC	61.79	29255136
1070	Phosphorylation	LNKGFKDTSQYVVGE HHCCCCCHHHHHHHH	20.51	-
1073	Phosphorylation	GFKDTSQYVVGELAA CCCCHHHHHHHHHHH	9.25	27642862
1097	Ubiquitination	TRAALVEKRLRYLMD HHHHHHHHHHHHHHH	49.61	-
1101	Phosphorylation	LVEKRLRYLMDTGRN HHHHHHHHHHHCCCC	15.80	-
1137	Phosphorylation	IRRMNQLSLLEKEHD HHHHHHHHHHHHHCC	22.57	24719451
1188	Ubiquitination	ELVALVNKRDALVRD HHHHHHHHHHHHHHH	44.14	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of EHBP1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of EHBP1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of EHBP1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
EHD2_HUMAN	EHD2	physical	14676205
ACAP2_HUMAN	ACAP2	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
611868	Prostate cancer, hereditary, 12 (HPC12)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of EHBP1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171; SER-174; SER-432;SER-436 AND SER-1058, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-426; THR-427; SER-428;SER-432; SER-436 AND SER-964, AND MASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-171; SER-174; SER-177;SER-428; SER-432; SER-436; SER-710; SER-964 AND SER-1058, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-426; SER-428 ANDSER-436, AND MASS SPECTROMETRY.	18220336 [Abstract]