dbPTM

S12A4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	S12A4_HUMAN
UniProt AC	Q9UP95
Protein Name	Solute carrier family 12 member 4
Gene Name	SLC12A4
Organism	Homo sapiens (Human).
Sequence Length	1085
Subcellular Localization	Membrane Multi-pass membrane protein.
Protein Description	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia (By similarity). Isoform 4 has no transport activity..
Protein Sequence	MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAELDDSDGHGNHRESSPFLSPLEASRGIDYYDRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGTRRRAAEAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGAHDTSNATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTAVVDNETVATQLWSFFCHSPNLTTDSCDPYFMLNNVTEIPGIPGAAAGVLQENLWSAYLEKGDIVEKHGLPSADAPSLKESLPLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIITTSLVYFSSVVLFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKVNGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNHERYLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQMDDNSIQMKKDLAVFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTEREREAQLVKDRHSALRLESLYSDEEDESAVGADKIQMTWTRDKYMTETWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPRNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4 (in isoform 5)	Phosphorylation	-	8.97	24719451
5	Phosphorylation	---MPHFTVVPVDGP ---CCCEEEEECCCC	19.98	-
6 (in isoform 5)	Phosphorylation	-	4.47	24719451
17	Phosphorylation	DGPRRGDYDNLEGLS CCCCCCCCCCCCCCC	15.00	28796482
24	Phosphorylation	YDNLEGLSWVDYGER CCCCCCCCCCCCCCC	35.59	26657352
28	Phosphorylation	EGLSWVDYGERAELD CCCCCCCCCCCEECC	15.97	27642862
37	Phosphorylation	ERAELDDSDGHGNHR CCEECCCCCCCCCCC	45.20	29255136
46	Phosphorylation	GHGNHRESSPFLSPL CCCCCCCCCCCCCHH	43.18	30266825
47	Phosphorylation	HGNHRESSPFLSPLE CCCCCCCCCCCCHHH	18.21	29255136
51	Phosphorylation	RESSPFLSPLEASRG CCCCCCCCHHHHHCC	28.02	25159151
56	Phosphorylation	FLSPLEASRGIDYYD CCCHHHHHCCCCHHH	22.97	30266825
61	Phosphorylation	EASRGIDYYDRNLAL HHHCCCCHHHHCHHH	12.82	25884760
62	Phosphorylation	ASRGIDYYDRNLALF HHCCCCHHHHCHHHH	12.51	28796482
78	Ubiquitination	EELDIRPKVSSLLGK HHHCCHHHHHHHHHH	44.92	21906983
78 (in isoform 1)	Ubiquitination	-	44.92	21890473
78 (in isoform 2)	Ubiquitination	-	44.92	21890473
78 (in isoform 3)	Ubiquitination	-	44.92	21890473
78 (in isoform 4)	Ubiquitination	-	44.92	21890473
79	Ubiquitination	ELDIRPKVSSLLGKL HHCCHHHHHHHHHHH	5.35	21890473
79	Ubiquitination	ELDIRPKVSSLLGKL HHCCHHHHHHHHHHH	5.35	21890473
79	Ubiquitination	ELDIRPKVSSLLGKL HHCCHHHHHHHHHHH	5.35	21890473
80	Phosphorylation	LDIRPKVSSLLGKLV HCCHHHHHHHHHHHH	22.76	29514088
81	Phosphorylation	DIRPKVSSLLGKLVS CCHHHHHHHHHHHHH	30.51	29514088
85	Acetylation	KVSSLLGKLVSYTNL HHHHHHHHHHHHCCC	45.76	26051181
85	Ubiquitination	KVSSLLGKLVSYTNL HHHHHHHHHHHHCCC	45.76	21890473
85 (in isoform 1)	Ubiquitination	-	45.76	21890473
85 (in isoform 2)	Ubiquitination	-	45.76	21890473
85 (in isoform 3)	Ubiquitination	-	45.76	21890473
85 (in isoform 4)	Ubiquitination	-	45.76	21890473
87	Ubiquitination	SSLLGKLVSYTNLTQ HHHHHHHHHHCCCCC	4.72	21890473
88	Phosphorylation	SLLGKLVSYTNLTQG HHHHHHHHHCCCCCC	36.35	21945579
89	Phosphorylation	LLGKLVSYTNLTQGA HHHHHHHHCCCCCCH	7.68	21945579
90	Phosphorylation	LGKLVSYTNLTQGAK HHHHHHHCCCCCCHH	19.08	21945579
93	Phosphorylation	LVSYTNLTQGAKEHE HHHHCCCCCCHHHHH	27.33	21945579
97	Ubiquitination	TNLTQGAKEHEEAES CCCCCCHHHHHHHHC	67.44	21906983
97 (in isoform 1)	Ubiquitination	-	67.44	21890473
97 (in isoform 2)	Ubiquitination	-	67.44	21890473
97 (in isoform 3)	Ubiquitination	-	67.44	21890473
97 (in isoform 4)	Ubiquitination	-	67.44	21890473
104	Phosphorylation	KEHEEAESGEGTRRR HHHHHHHCCCCHHHH	49.55	26699800
108	Phosphorylation	EAESGEGTRRRAAEA HHHCCCCHHHHHHCC	19.39	26699800
245	N-linked_Glycosylation	SGAHDTSNATLNNMR CCCCCCCCCHHHCCC	38.75	UniProtKB CARBOHYD
260	Phosphorylation	VYGTIFLTFMTLVVF HHHHHHHHHHHHHHH	10.70	-
263	Phosphorylation	TIFLTFMTLVVFVGV HHHHHHHHHHHHHCH	16.53	-
312	N-linked_Glycosylation	FPVCMLGNRTLSRDQ CCEEECCCCCCCHHH	29.78	UniProtKB CARBOHYD
331	N-linked_Glycosylation	AKTAVVDNETVATQL CEEEEECCHHHHHHH	34.92	UniProtKB CARBOHYD
347	N-linked_Glycosylation	SFFCHSPNLTTDSCD HHHCCCCCCCCCCCC	53.70	UniProtKB CARBOHYD
407	Phosphorylation	DAPSLKESLPLYVVA CCCCHHHCCCHHHHH	33.43	-
411	Phosphorylation	LKESLPLYVVADIAT HHHCCCHHHHHHHHH	7.31	-
418	Phosphorylation	YVVADIATSFTVLVG HHHHHHHHHHHHHHH	25.38	-
439	N-linked_Glycosylation	TGIMAGSNRSGDLRD CEECCCCCCCCCHHH	41.02	UniProtKB CARBOHYD
441	Phosphorylation	IMAGSNRSGDLRDAQ ECCCCCCCCCHHHHH	40.69	-
498	Phosphorylation	SRNLVVGTLAWPSPW CCCEEEECCCCCCCE	11.16	-
529	Ubiquitination	SLTGAPRLLQAIAKD HHHCHHHHHHHHHHC	3.96	21890473
529	Ubiquitination	SLTGAPRLLQAIAKD HHHCHHHHHHHHHHC	3.96	21890473
529	Ubiquitination	SLTGAPRLLQAIAKD HHHCHHHHHHHHHHC	3.96	21890473
535	Acetylation	RLLQAIAKDNIIPFL HHHHHHHHCCCCCEE	45.23	42369537
535	Ubiquitination	RLLQAIAKDNIIPFL HHHHHHHHCCCCCEE	45.23	-
535 (in isoform 1)	Ubiquitination	-	45.23	21890473
535 (in isoform 2)	Ubiquitination	-	45.23	21890473
535 (in isoform 3)	Ubiquitination	-	45.23	21890473
535 (in isoform 4)	Ubiquitination	-	45.23	21890473
537	Ubiquitination	LQAIAKDNIIPFLRV HHHHHHCCCCCEEEH	33.12	21890473
659	Ubiquitination	IEYQGAEKEWGDGIR HHHCCCCCCCCCCHH	59.23	-
659 (in isoform 1)	Ubiquitination	-	59.23	21890473
659 (in isoform 2)	Ubiquitination	-	59.23	21890473
659 (in isoform 3)	Ubiquitination	-	59.23	21890473
659 (in isoform 4)	Ubiquitination	-	59.23	21890473
669	Phosphorylation	GDGIRGLSLSAARYA CCCHHHHHHHHHHHH	24.09	30108239
671	Phosphorylation	GIRGLSLSAARYALL CHHHHHHHHHHHHHH	19.26	30108239
701	Ubiquitination	LLVLLKLDEDLHVKY EEEEEECCCCCCCCC	45.86	21890473
701	Ubiquitination	LLVLLKLDEDLHVKY EEEEEECCCCCCCCC	45.86	21890473
701	Ubiquitination	LLVLLKLDEDLHVKY EEEEEECCCCCCCCC	45.86	21890473
707	Ubiquitination	LDEDLHVKYPRLLTF CCCCCCCCCHHHHHH	38.50	21890473
707 (in isoform 1)	Ubiquitination	-	38.50	21890473
707 (in isoform 2)	Ubiquitination	-	38.50	21890473
707 (in isoform 3)	Ubiquitination	-	38.50	21890473
707 (in isoform 4)	Ubiquitination	-	38.50	21890473
708	Phosphorylation	DEDLHVKYPRLLTFA CCCCCCCCHHHHHHH	7.78	22817900
709	Ubiquitination	EDLHVKYPRLLTFAS CCCCCCCHHHHHHHH	18.09	21890473
716	Phosphorylation	PRLLTFASQLKAGKG HHHHHHHHHHHCCCC	31.67	-
719	Ubiquitination	LTFASQLKAGKGLTI HHHHHHHHCCCCCEE	47.67	-
725	Phosphorylation	LKAGKGLTIVGSVIQ HHCCCCCEEEEEEEE	23.45	-
757	Methylation	KNMMEIEKVKGFCQV HHHHHHHHHCCCCCH	55.71	-
769	Malonylation	CQVVVASKVREGLAH CCHHHHHHHHHHHHH	35.26	26320211
836	Phosphorylation	KNIAFYPSNHERYLE CCEEECCCCCHHHHC	39.17	25159151
926	Phosphorylation	SAYTYERTLMMEQRS HHHHHHHHHHHHHHH	13.68	23401153
943	Acetylation	LRQMRLTKTEREREA HHHHHCCHHHHHHHH	53.64	7668913
954	Ubiquitination	EREAQLVKDRHSALR HHHHHHHHHHHHHHH	58.60	-
958	Phosphorylation	QLVKDRHSALRLESL HHHHHHHHHHHHHHH	29.87	30576142
964	Phosphorylation	HSALRLESLYSDEED HHHHHHHHHCCCCCC	36.88	22167270
966	Phosphorylation	ALRLESLYSDEEDES HHHHHHHCCCCCCCC	24.29	29255136
967	Phosphorylation	LRLESLYSDEEDESA HHHHHHCCCCCCCCC	43.79	19664994
969	Phosphorylation	LESLYSDEEDESAVG HHHHCCCCCCCCCCC	62.50	27251275
973	Phosphorylation	YSDEEDESAVGADKI CCCCCCCCCCCCCEE	40.34	22167270
982 (in isoform 4)	Ubiquitination	-	3.73	21890473
983	Phosphorylation	GADKIQMTWTRDKYM CCCEEEEEEECCHHC	13.81	30266825
985	Phosphorylation	DKIQMTWTRDKYMTE CEEEEEEECCHHCCC	21.59	30266825
988	Ubiquitination	QMTWTRDKYMTETWD EEEEECCHHCCCCCC	33.36	21906983
988 (in isoform 1)	Ubiquitination	-	33.36	21890473
988 (in isoform 2)	Ubiquitination	-	33.36	21890473
988 (in isoform 3)	Ubiquitination	-	33.36	21890473
989	Phosphorylation	MTWTRDKYMTETWDP EEEECCHHCCCCCCC	17.35	20049867
991	Phosphorylation	WTRDKYMTETWDPSH EECCHHCCCCCCCCC	27.46	-
993	Phosphorylation	RDKYMTETWDPSHAP CCHHCCCCCCCCCCC	26.46	-
1004 (in isoform 4)	Ubiquitination	-	42.46	21890473
1010	Ubiquitination	FRELVHIKPDQSNVR CHHHEECCCCCCCCH	27.88	2190698
1010 (in isoform 1)	Ubiquitination	-	27.88	21890473
1010 (in isoform 2)	Ubiquitination	-	27.88	21890473
1014	Phosphorylation	VHIKPDQSNVRRMHT EECCCCCCCCHHHHH	44.68	20049867
1021	Phosphorylation	SNVRRMHTAVKLNEV CCCHHHHHEEECCEE	24.65	-
1056	Phosphorylation	NSEGDENYMEFLEVL CCCCCCCHHHHHHHH	8.93	10942735
1064	Phosphorylation	MEFLEVLTEGLERVL HHHHHHHHHCCCEEE	32.93	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
5	T	Phosphorylation	Kinase	STK39	Q9UEW8	GPS
983	T	Phosphorylation	Kinase	STK39	Q9UEW8	GPS

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of S12A4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of S12A4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
TMM43_HUMAN	TMEM43	physical	22939629

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
DB00887	Bumetanide
DB00761	Potassium Chloride

Regulatory Network of S12A4_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-967, AND MASSSPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51 AND SER-967, AND MASSSPECTROMETRY.	18669648 [Abstract]