dbPTM

SSH2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SSH2_HUMAN
UniProt AC	Q76I76
Protein Name	Protein phosphatase Slingshot homolog 2
Gene Name	SSH2
Organism	Homo sapiens (Human).
Sequence Length	1423
Subcellular Localization	Cytoplasm, cytoskeleton.
Protein Description	Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein..
Protein Sequence	MALVTVQRSPTPSTTSSPCASEADSGEEECRSQPRSISESFLTVKGAALFLPRGNGSSTPRISHRRNKHAGDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDSSTCTMGLVLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWSALQSLHKACEVARAHNYYPGSLFLTWVSYYESHINSDQSSVNEWNAMQDVQSHRPDSPALFTDIPTERERTERLIKTKLREIMMQKDLENITSKEIRTELEMQMVCNLREFKEFIDNEMIVILGQMDSPTQIFEHVFLGSEWNASNLEDLQNRGVRYILNVTREIDNFFPGVFEYHNIRVYDEEATDLLAYWNDTYKFISKAKKHGSKCLVHCKMGVSRSASTVIAYAMKEYGWNLDRAYDYVKERRTVTKPNPSFMRQLEEYQGILLASKQRHNKLWRSHSDSDLSDHHEPICKPGLELNKKDITTSADQIAEVKTMESHPPIPPVFVEHMVPQDANQKGLCTKERMICLEFTSREFHAGQIEDELNLNDINGCSSGCCLNESKFPLDNCHASKALIQPGHVPEMANKFPDLTVEDLETDALKADMNVHLLPMEELTSPLKDPPMSPDPESPSPQPSCQTEISDFSTDRIDFFSALEKFVELSQETRSRSFSHSRMEELGGGRNESCRLSVVEVAPSKVTADDQRSSSLSNTPHASEESSMDEEQSKAISELVSPDIFMQSHSENAISVKEIVTEIESISQGVGQIQLKGDILPNPCHTPKKNSIHELLLERAQTPENKPGHMEQDEDSCTAQPELAKDSGMCNPEGCLTTHSSIADLEEGEPAEGEQELQGSGMHPGAKWYPGSVRRATLEFEERLRQEQEHHGAAPTCTSLSTRKNSKNDSSVADLAPKGKSDEAPPEHSFVLKEPEMSKGKGKYSGSEAGSLSHSEQNATVPAPRVLEFDHLPDPQEGPGSDTGTQQEGVLKDLRTVIPYQESETQAVPLPLPKRVEIIEYTHIVTSPNHTGPGSEIATSEKSGEQGLRKVNMEKSVTVLCTLDENLNRTLDPNQVSLHPQVLPLPHSSSPEHNRPTDHPTSILSSPEDRGSSLSTALETAAPFVSHTTHLLSASLDYLHPQTMVHLEGFTEQSSTTDEPSAEQVSWEESQESPLSSGSEVPYKDSQLSSADLSLISKLGDNTGELQEKMDPLPVACRLPHSSSSENIKSLSHSPGVVKERAKEIESRVVFQAGLTKPSQMRRSASLAKLGYLDLCKDCLPEREPASCESPHLKLLQPFLRTDSGMHAMEDQESLENPGAPHNPEPTKSFVEQLTTTECIVQSKPVERPLVQYAKEFGSSQQYLLPRAGLELTSSEGGLPVLQTQGLQCACPAPGLAVAPRQQHGRTHPLRRLKKANDKKRTTNPFYNTM

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
13	Phosphorylation	VQRSPTPSTTSSPCA EECCCCCCCCCCCCC	46.96	28450419
14	Phosphorylation	QRSPTPSTTSSPCAS ECCCCCCCCCCCCCC	31.72	28450419
15	Phosphorylation	RSPTPSTTSSPCASE CCCCCCCCCCCCCCC	31.38	28450419
16	Phosphorylation	SPTPSTTSSPCASEA CCCCCCCCCCCCCCC	32.16	28450419
17	Phosphorylation	PTPSTTSSPCASEAD CCCCCCCCCCCCCCC	23.16	28450419
21	Phosphorylation	TTSSPCASEADSGEE CCCCCCCCCCCCCCH	39.78	28450419
25	Phosphorylation	PCASEADSGEEECRS CCCCCCCCCCHHHHC	55.97	28450419
32	Phosphorylation	SGEEECRSQPRSISE CCCHHHHCCCCCCCH	57.08	28450419
36	Phosphorylation	ECRSQPRSISESFLT HHHCCCCCCCHHHHE	36.83	30266825
38	Phosphorylation	RSQPRSISESFLTVK HCCCCCCCHHHHEEC	28.56	30266825
40	Phosphorylation	QPRSISESFLTVKGA CCCCCCHHHHEECEE	20.83	23927012
43	Phosphorylation	SISESFLTVKGAALF CCCHHHHEECEEEEE	20.40	23927012
63	Phosphorylation	GSSTPRISHRRNKHA CCCCCCCCCCCCCCC	16.54	-
134	Phosphorylation	DFSSNDSSTCTMGLV ECCCCCCCCCEEEEE	30.30	25332170
135	Phosphorylation	FSSNDSSTCTMGLVL CCCCCCCCCEEEEEE	18.89	25332170
148	Phosphorylation	VLPLWSDTLIHLDGD EEEEECCEEEEECCC	23.30	25332170
231	Phosphorylation	NAMQDVQSHRPDSPA HHHHHHHHCCCCCCC	22.70	26074081
236	Phosphorylation	VQSHRPDSPALFTDI HHHCCCCCCCCCCCC	18.11	26074081
241	Phosphorylation	PDSPALFTDIPTERE CCCCCCCCCCCCHHH	33.59	26074081
245	Phosphorylation	ALFTDIPTERERTER CCCCCCCCHHHHHHH	48.27	26074081
255	Acetylation	ERTERLIKTKLREIM HHHHHHHHHHHHHHH	44.17	25953088
265	Ubiquitination	LREIMMQKDLENITS HHHHHCHHCHHCCCC	46.51	-
273	Ubiquitination	DLENITSKEIRTELE CHHCCCCHHHHHHHH	48.81	-
399	Phosphorylation	CKMGVSRSASTVIAY CCCCCCCCHHHHHHH	21.04	27251275
401	Phosphorylation	MGVSRSASTVIAYAM CCCCCCHHHHHHHHH	25.18	-
402	Phosphorylation	GVSRSASTVIAYAMK CCCCCHHHHHHHHHH	18.69	-
406	Phosphorylation	SASTVIAYAMKEYGW CHHHHHHHHHHHHCC	8.98	-
411	Phosphorylation	IAYAMKEYGWNLDRA HHHHHHHHCCCHHHH	23.17	22817900
419	Phosphorylation	GWNLDRAYDYVKERR CCCHHHHHHHHHHCC	14.54	22817900
421	Phosphorylation	NLDRAYDYVKERRTV CHHHHHHHHHHCCCC	10.76	22817900
423	Ubiquitination	DRAYDYVKERRTVTK HHHHHHHHHCCCCCC	39.00	-
430	Ubiquitination	KERRTVTKPNPSFMR HHCCCCCCCCHHHHH	38.16	-
450	Ubiquitination	QGILLASKQRHNKLW HHHHHHHHHHCCCHH	45.82	-
455	Ubiquitination	ASKQRHNKLWRSHSD HHHHHCCCHHHCCCC	43.21	-
459	Phosphorylation	RHNKLWRSHSDSDLS HCCCHHHCCCCCCCC	18.76	23927012
461	Phosphorylation	NKLWRSHSDSDLSDH CCHHHCCCCCCCCCC	39.80	23927012
463	Phosphorylation	LWRSHSDSDLSDHHE HHHCCCCCCCCCCCC	43.39	23927012
466	Phosphorylation	SHSDSDLSDHHEPIC CCCCCCCCCCCCCCC	39.63	23927012
482	Ubiquitination	PGLELNKKDITTSAD CCCCCCHHHCCCCHH	53.97	-
485	Phosphorylation	ELNKKDITTSADQIA CCCHHHCCCCHHHHH	25.49	30266825
486	Phosphorylation	LNKKDITTSADQIAE CCHHHCCCCHHHHHH	23.42	30266825
487	Phosphorylation	NKKDITTSADQIAEV CHHHCCCCHHHHHHH	22.62	19664994
534	Phosphorylation	MICLEFTSREFHAGQ EEEEEECCCCCCCCC	34.82	-
588	Ubiquitination	HVPEMANKFPDLTVE CCHHHHHHCCCCCHH	49.05	-
617	Phosphorylation	LLPMEELTSPLKDPP ECCHHHCCCCCCCCC	31.42	28634298
618	Phosphorylation	LPMEELTSPLKDPPM CCHHHCCCCCCCCCC	40.64	24719451
626	Phosphorylation	PLKDPPMSPDPESPS CCCCCCCCCCCCCCC	31.74	28348404
631	Phosphorylation	PMSPDPESPSPQPSC CCCCCCCCCCCCCCC	35.75	28348404
633	Phosphorylation	SPDPESPSPQPSCQT CCCCCCCCCCCCCCC	45.50	28348404
654	Phosphorylation	TDRIDFFSALEKFVE CCCHHHHHHHHHHHH	31.75	-
666	Phosphorylation	FVELSQETRSRSFSH HHHHCHHHHHCCCCH	26.72	-
668	Phosphorylation	ELSQETRSRSFSHSR HHCHHHHHCCCCHHH	39.43	28348404
670	Phosphorylation	SQETRSRSFSHSRME CHHHHHCCCCHHHHH	32.14	28348404
672	Phosphorylation	ETRSRSFSHSRMEEL HHHHCCCCHHHHHHH	23.07	28122231
674	Phosphorylation	RSRSFSHSRMEELGG HHCCCCHHHHHHHCC	31.93	28122231
686	Phosphorylation	LGGGRNESCRLSVVE HCCCCCCCCCEEEEE	14.21	28122231
690	O-linked_Glycosylation	RNESCRLSVVEVAPS CCCCCCEEEEEECCC	12.55	30379171
690	Phosphorylation	RNESCRLSVVEVAPS CCCCCCEEEEEECCC	12.55	28111955
698	Acetylation	VVEVAPSKVTADDQR EEEECCCCCCCCCCC	42.41	25953088
706	Phosphorylation	VTADDQRSSSLSNTP CCCCCCCCCCCCCCC	20.74	29978859
707	Phosphorylation	TADDQRSSSLSNTPH CCCCCCCCCCCCCCC	37.17	29978859
708	Phosphorylation	ADDQRSSSLSNTPHA CCCCCCCCCCCCCCC	36.74	29978859
710	Phosphorylation	DQRSSSLSNTPHASE CCCCCCCCCCCCCCC	40.37	29978859
712	Phosphorylation	RSSSLSNTPHASEES CCCCCCCCCCCCCCC	17.05	29978859
716	Phosphorylation	LSNTPHASEESSMDE CCCCCCCCCCCCCCH	38.57	23312004
719	Phosphorylation	TPHASEESSMDEEQS CCCCCCCCCCCHHHH	27.23	23312004
720	Phosphorylation	PHASEESSMDEEQSK CCCCCCCCCCHHHHH	33.27	23312004
730	Phosphorylation	EEQSKAISELVSPDI HHHHHHHHHHHCHHH	29.72	20068231
734	Phosphorylation	KAISELVSPDIFMQS HHHHHHHCHHHHHCC	29.49	20068231
741	Phosphorylation	SPDIFMQSHSENAIS CHHHHHCCCCCCCCC	19.72	20068231
743	Phosphorylation	DIFMQSHSENAISVK HHHHCCCCCCCCCHH	37.48	20068231
754	Phosphorylation	ISVKEIVTEIESISQ CCHHHHHHHHHHHHC	36.59	-
758	Phosphorylation	EIVTEIESISQGVGQ HHHHHHHHHHCCCCE	32.95	-
760	Phosphorylation	VTEIESISQGVGQIQ HHHHHHHHCCCCEEE	30.11	-
779	Phosphorylation	ILPNPCHTPKKNSIH CCCCCCCCCCCCCHH	43.45	30266825
782	Ubiquitination	NPCHTPKKNSIHELL CCCCCCCCCCHHHHH	58.07	-
784	Phosphorylation	CHTPKKNSIHELLLE CCCCCCCCHHHHHHH	33.91	23401153
795	Phosphorylation	LLLERAQTPENKPGH HHHHHCCCCCCCCCC	31.42	23401153
809	Phosphorylation	HMEQDEDSCTAQPEL CCCCCCCCCCCCHHH	16.00	23403867
811	Phosphorylation	EQDEDSCTAQPELAK CCCCCCCCCCHHHHH	32.07	23403867
870	Phosphorylation	PGSVRRATLEFEERL CCCCCHHHHHHHHHH	25.63	28985074
894	Phosphorylation	APTCTSLSTRKNSKN CCCCCCCCCCCCCCC	26.40	28555341
899	Phosphorylation	SLSTRKNSKNDSSVA CCCCCCCCCCCCCHH	35.55	23403867
903	Phosphorylation	RKNSKNDSSVADLAP CCCCCCCCCHHHHCC	35.78	23403867
904	Phosphorylation	KNSKNDSSVADLAPK CCCCCCCCHHHHCCC	24.96	23403867
931	Phosphorylation	VLKEPEMSKGKGKYS EECCCCCCCCCCCCC	36.27	28258704
936	Acetylation	EMSKGKGKYSGSEAG CCCCCCCCCCCCCCC	39.46	25953088
985	Ubiquitination	TQQEGVLKDLRTVIP CCCCHHHCHHCCCCC	52.92	-
989	Phosphorylation	GVLKDLRTVIPYQES HHHCHHCCCCCCCCC	30.04	22468782
993	Phosphorylation	DLRTVIPYQESETQA HHCCCCCCCCCCCCC	17.49	22468782
996	Phosphorylation	TVIPYQESETQAVPL CCCCCCCCCCCCCCC	30.34	22468782
1019	Phosphorylation	IEYTHIVTSPNHTGP EEEEEEECCCCCCCC	37.55	23898821
1020	Phosphorylation	EYTHIVTSPNHTGPG EEEEEECCCCCCCCC	16.89	28122231
1024	Phosphorylation	IVTSPNHTGPGSEIA EECCCCCCCCCCCCC	52.27	28122231
1028	Phosphorylation	PNHTGPGSEIATSEK CCCCCCCCCCCCCCC	29.03	28122231
1081	Phosphorylation	QVLPLPHSSSPEHNR CEECCCCCCCCCCCC	30.48	26074081
1082	Phosphorylation	VLPLPHSSSPEHNRP EECCCCCCCCCCCCC	47.05	26074081
1083	Phosphorylation	LPLPHSSSPEHNRPT ECCCCCCCCCCCCCC	37.00	26074081
1090	Phosphorylation	SPEHNRPTDHPTSIL CCCCCCCCCCCCCCC	43.64	28464451
1094	Phosphorylation	NRPTDHPTSILSSPE CCCCCCCCCCCCCCC	25.61	28348404
1095	Phosphorylation	RPTDHPTSILSSPED CCCCCCCCCCCCCCC	26.44	28348404
1098	Phosphorylation	DHPTSILSSPEDRGS CCCCCCCCCCCCCCC	42.02	28348404
1099	Phosphorylation	HPTSILSSPEDRGSS CCCCCCCCCCCCCCC	28.94	28348404
1169	Phosphorylation	ESQESPLSSGSEVPY HHCCCCCCCCCCCCC	35.72	28787133
1170	Phosphorylation	SQESPLSSGSEVPYK HCCCCCCCCCCCCCC	54.76	28787133
1182	Phosphorylation	PYKDSQLSSADLSLI CCCCCCCCHHHHHHH	18.82	30576142
1183	Phosphorylation	YKDSQLSSADLSLIS CCCCCCCHHHHHHHH	34.10	30576142
1187	Phosphorylation	QLSSADLSLISKLGD CCCHHHHHHHHHHCC	24.97	21815630
1191	Ubiquitination	ADLSLISKLGDNTGE HHHHHHHHHCCCCHH	49.88	-
1215	Phosphorylation	VACRLPHSSSSENIK EEECCCCCCCCCCHH	29.94	23927012
1216	Phosphorylation	ACRLPHSSSSENIKS EECCCCCCCCCCHHH	34.54	30278072
1217	Phosphorylation	CRLPHSSSSENIKSL ECCCCCCCCCCHHHH	44.69	23401153
1218	Phosphorylation	RLPHSSSSENIKSLS CCCCCCCCCCHHHHC	36.29	23927012
1223	Phosphorylation	SSSENIKSLSHSPGV CCCCCHHHHCCCCCH	31.16	30266825
1225	Phosphorylation	SENIKSLSHSPGVVK CCCHHHHCCCCCHHH	29.07	30266825
1227	Phosphorylation	NIKSLSHSPGVVKER CHHHHCCCCCHHHHH	21.70	30266825
1249	Phosphorylation	VVFQAGLTKPSQMRR HHHHCCCCCHHHHHH	39.74	-
1252	Phosphorylation	QAGLTKPSQMRRSAS HCCCCCHHHHHHHHH	37.86	-
1257	Phosphorylation	KPSQMRRSASLAKLG CHHHHHHHHHHHHHH	16.31	28102081
1259	Phosphorylation	SQMRRSASLAKLGYL HHHHHHHHHHHHHHH	30.06	23312004
1280	Phosphorylation	LPEREPASCESPHLK CCCCCCCCCCCCCHH	29.18	23401153
1283	Phosphorylation	REPASCESPHLKLLQ CCCCCCCCCCHHHHH	23.19	25159151
1295	Phosphorylation	LLQPFLRTDSGMHAM HHHHHHHCCCCCCCC	36.34	28450419
1297	Phosphorylation	QPFLRTDSGMHAMED HHHHHCCCCCCCCCC	37.09	28450419
1307	Phosphorylation	HAMEDQESLENPGAP CCCCCHHHHCCCCCC	35.04	29978859
1352	Phosphorylation	QYAKEFGSSQQYLLP HHHHHHCCCCCEEEC	30.23	-
1356	Phosphorylation	EFGSSQQYLLPRAGL HHCCCCCEEECCCCC	11.54	28796482
1400	Phosphorylation	PRQQHGRTHPLRRLK CHHHCCCCCHHHHHH	32.22	28152594
1408	Acetylation	HPLRRLKKANDKKRT CHHHHHHHHCCCCCC	57.95	7377565
1412	Acetylation	RLKKANDKKRTTNPF HHHHHCCCCCCCCCC	43.92	7377575
1413	Acetylation	LKKANDKKRTTNPFY HHHHCCCCCCCCCCC	58.82	7377585
1415	Phosphorylation	KANDKKRTTNPFYNT HHCCCCCCCCCCCCC	39.54	26074081
1416	Phosphorylation	ANDKKRTTNPFYNTM HCCCCCCCCCCCCCC	44.95	26074081
1420	Phosphorylation	KRTTNPFYNTM---- CCCCCCCCCCC----	15.81	23403867
1422	Phosphorylation	TTNPFYNTM------ CCCCCCCCC------	16.93	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SSH2_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SSH2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SSH2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
COF1_MOUSE	Cfl1	physical	11832213
MYO1D_HUMAN	MYO1D	physical	27880917

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SSH2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36; SER-487 ANDTHR-1422, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-487, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36 AND THR-1422, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-461, AND MASSSPECTROMETRY.	17081983 [Abstract]