DDX42_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: DDX42_HUMAN
• UniProt AC: Q86XP3
• Protein Name: ATP-dependent RNA helicase DDX42
• Gene Name: DDX42
• Organism: Homo sapiens (Human).
• Sequence Length: 938
• Subcellular Localization: Cytoplasm. Nucleus speckle. Nucleus, Cajal body. Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles.
• Protein Description: ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm..
• Protein Sequence: MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFDEENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKERKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDYPPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFGKAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTRRLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAARGLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MNWNKGGP -------CCCCCCCC	13.63	-
5	Acetylation	---MNWNKGGPGTKR ---CCCCCCCCCCCC	57.78	19608861
10	Phosphorylation	WNKGGPGTKRGFGFG CCCCCCCCCCCCCCC	22.24	22817900
12	Methylation	KGGPGTKRGFGFGGF CCCCCCCCCCCCCCE	45.57	24129315
22	Phosphorylation	GFGGFAISAGKKEEP CCCCEEEECCCCCCC	27.78	28555341
25	Acetylation	GFAISAGKKEEPKLP CEEEECCCCCCCCCC	57.64	25953088
26	Acetylation	FAISAGKKEEPKLPQ EEEECCCCCCCCCCC	67.41	26051181
30	Acetylation	AGKKEEPKLPQQSHS CCCCCCCCCCCCCCC	75.88	30583609
30	Ubiquitination	AGKKEEPKLPQQSHS CCCCCCCCCCCCCCC	75.88	29967540
37	Phosphorylation	KLPQQSHSAFGATSS CCCCCCCCCCCCCCC	30.46	28555341
42	Phosphorylation	SHSAFGATSSSSGFG CCCCCCCCCCCCCCC	29.53	28555341
43	O-linked_Glycosylation	HSAFGATSSSSGFGK CCCCCCCCCCCCCCC	27.91	30059200
43	Phosphorylation	HSAFGATSSSSGFGK CCCCCCCCCCCCCCC	27.91	28555341
44	Phosphorylation	SAFGATSSSSGFGKS CCCCCCCCCCCCCCC	24.94	25627689
45	Phosphorylation	AFGATSSSSGFGKSA CCCCCCCCCCCCCCC	33.96	25627689
46	Phosphorylation	FGATSSSSGFGKSAP CCCCCCCCCCCCCCC	39.84	28555341
50	Acetylation	SSSSGFGKSAPPQLP CCCCCCCCCCCCCCC	40.98	26051181
50	Methylation	SSSSGFGKSAPPQLP CCCCCCCCCCCCCCC	40.98	-
51	Phosphorylation	SSSGFGKSAPPQLPS CCCCCCCCCCCCCCC	46.52	23186163
58	Phosphorylation	SAPPQLPSFYKIGSK CCCCCCCCCEEECCC	50.21	23917254
60	Phosphorylation	PPQLPSFYKIGSKRA CCCCCCCEEECCCCC	13.16	27080861
75	Phosphorylation	NFDEENAYFEDEEED CCCHHHCCCCCCCCC	21.05	30576142
83	Phosphorylation	FEDEEEDSSNVDLPY CCCCCCCCCCCCCCC	27.39	30266825
84	Phosphorylation	EDEEEDSSNVDLPYI CCCCCCCCCCCCCCC	53.87	30266825
90	Phosphorylation	SSNVDLPYIPAENSP CCCCCCCCCCCCCCC	27.17	30266825
96	Phosphorylation	PYIPAENSPTRQQFH CCCCCCCCCCCHHHC	21.10	25159151
98	Phosphorylation	IPAENSPTRQQFHSK CCCCCCCCCHHHCCC	40.71	30266825
104	Phosphorylation	PTRQQFHSKPVDSDS CCCHHHCCCCCCCCC	39.69	21955146
109	Phosphorylation	FHSKPVDSDSDDDPL HCCCCCCCCCCCCHH	38.97	25159151
111	Phosphorylation	SKPVDSDSDDDPLEA CCCCCCCCCCCHHHH	47.06	25159151
145	Acetylation	DKERKNVKGIRDDIE HHHHHCCCCCCCCCC	59.00	23236377
160	Phosphorylation	EEDDQEAYFRYMAEN CCCCHHHHHHHHHHC	6.45	22817900
163	Phosphorylation	DQEAYFRYMAENPTA CHHHHHHHHHHCCCC	7.26	23927012
169	Phosphorylation	RYMAENPTAGVVQEE HHHHHCCCCCCCCCH	47.85	30266825
183	Phosphorylation	EEEDNLEYDSDGNPI HHCCCCCCCCCCCCC	24.74	29255136
185	Phosphorylation	EDNLEYDSDGNPIAP CCCCCCCCCCCCCCC	46.66	29255136
193	Phosphorylation	DGNPIAPTKKIIDPL CCCCCCCCCCCCCCC	35.63	30266825
195	Ubiquitination	NPIAPTKKIIDPLPP CCCCCCCCCCCCCCC	47.58	29967540
206	Phosphorylation	PLPPIDHSEIDYPPF CCCCCCHHHCCCCCC	32.28	24247654
210	Phosphorylation	IDHSEIDYPPFEKNF CCHHHCCCCCCCCCC	20.61	20873877
215	Ubiquitination	IDYPPFEKNFYNEHE CCCCCCCCCCCCCCH	53.38	29967540
225	Phosphorylation	YNEHEEITNLTPQQL CCCCHHHHCCCHHHH	27.70	27732954
228	Phosphorylation	HEEITNLTPQQLIDL CHHHHCCCHHHHHHH	22.56	25159151
270	Sumoylation	QLMHQIRKSEYTQPT HHHHHHHHCCCCCCC	49.63	-
270	Acetylation	QLMHQIRKSEYTQPT HHHHHHHHCCCCCCC	49.63	26051181
270	Malonylation	QLMHQIRKSEYTQPT HHHHHHHHCCCCCCC	49.63	26320211
270	Sumoylation	QLMHQIRKSEYTQPT HHHHHHHHCCCCCCC	49.63	-
270	Ubiquitination	QLMHQIRKSEYTQPT HHHHHHHHCCCCCCC	49.63	29967540
271	Phosphorylation	LMHQIRKSEYTQPTP HHHHHHHCCCCCCCC	26.45	28152594
273	Phosphorylation	HQIRKSEYTQPTPIQ HHHHHCCCCCCCCCE	20.12	28152594
274	Phosphorylation	QIRKSEYTQPTPIQC HHHHCCCCCCCCCEE	24.83	28152594
277	Phosphorylation	KSEYTQPTPIQCQGV HCCCCCCCCCEECCE	24.17	28152594
298	2-Hydroxyisobutyrylation	RDMIGIAKTGSGKTA CCEEEEEECCCCHHH	52.04	-
298	Acetylation	RDMIGIAKTGSGKTA CCEEEEEECCCCHHH	52.04	25953088
298	Ubiquitination	RDMIGIAKTGSGKTA CCEEEEEECCCCHHH	52.04	-
341	Ubiquitination	PTRELCQQIHAECKR CHHHHHHHHHHHHHH	28.56	24816145
347	Acetylation	QQIHAECKRFGKAYN HHHHHHHHHHCHHCC	42.12	25953088
351	Acetylation	AECKRFGKAYNLRSV HHHHHHCHHCCCCEE	45.01	26051181
383	Phosphorylation	GAEIVVCTPGRLIDH CCEEEEECCCHHHHH	19.71	27690223
392	2-Hydroxyisobutyrylation	GRLIDHVKKKATNLQ CHHHHHHHHHCCCCC	45.17	-
422	Ubiquitination	MGFEYQVRSIASHVR CCCHHHHHHHHHHCC	13.56	24816145
442	Ubiquitination	LLFSATFRKKIEKLA EEEEHHHHHHHHHHH	34.70	24816145
519	Acetylation	EELANNLKQEGHNLG HHHHHHHHHHCCCHH	48.72	26051181
523	Ubiquitination	NNLKQEGHNLGLLHG HHHHHHCCCHHCCCC	26.11	24816145
545	2-Hydroxyisobutyrylation	NKVISDFKKKDIPVL HHHHHHHHHCCCCEE	65.00	-
545	Acetylation	NKVISDFKKKDIPVL HHHHHHHHHCCCCEE	65.00	25953088
555	Phosphorylation	DIPVLVATDVAARGL CCCEEEEECHHHCCC	24.43	21406692
566	Phosphorylation	ARGLDIPSIKTVINY HCCCCCCCCCEEEEH	36.73	24719451
581	Phosphorylation	DVARDIDTHTHRIGR HHHCCCCCCCCCCCC	29.07	20068231
583	Phosphorylation	ARDIDTHTHRIGRTG HCCCCCCCCCCCCCC	18.23	20068231
599	Phosphorylation	AGEKGVAYTLLTPKD CCCCCEEEEEECCCC	8.77	28152594
600	Phosphorylation	GEKGVAYTLLTPKDS CCCCEEEEEECCCCC	12.93	28152594
603	Phosphorylation	GVAYTLLTPKDSNFA CEEEEEECCCCCCCC	31.25	28857561
605	Ubiquitination	AYTLLTPKDSNFAGD EEEEECCCCCCCCHH	69.43	24816145
626	Acetylation	GANQHVSKELLDLAM HHHHHHCHHHHHHHH	51.82	7713437
668	Phosphorylation	RERPGLGSENMDRGN CCCCCCCCCCCCCCC	30.73	20873877
671	Sulfoxidation	PGLGSENMDRGNNNV CCCCCCCCCCCCCCC	3.05	21406390
673	Methylation	LGSENMDRGNNNVMS CCCCCCCCCCCCCCC	38.68	-
680	Phosphorylation	RGNNNVMSNYEAYKP CCCCCCCCCCEECCC	31.79	22210691
682	Phosphorylation	NNNVMSNYEAYKPST CCCCCCCCEECCCCC	8.47	21945579
685	Phosphorylation	VMSNYEAYKPSTGAM CCCCCEECCCCCCCC	16.11	22210691
686	Acetylation	MSNYEAYKPSTGAMG CCCCEECCCCCCCCH	39.27	23954790
686	Ubiquitination	MSNYEAYKPSTGAMG CCCCEECCCCCCCCH	39.27	24816145
688	Phosphorylation	NYEAYKPSTGAMGDR CCEECCCCCCCCHHH	35.01	21945579
689	Phosphorylation	YEAYKPSTGAMGDRL CEECCCCCCCCHHHH	37.17	21945579
700	Acetylation	GDRLTAMKAAFQSQY HHHHHHHHHHHHHHH	33.81	25953088
700	Methylation	GDRLTAMKAAFQSQY HHHHHHHHHHHHHHH	33.81	-
700	Ubiquitination	GDRLTAMKAAFQSQY HHHHHHHHHHHHHHH	33.81	32015554
705	Phosphorylation	AMKAAFQSQYKSHFV HHHHHHHHHHHHHHH	29.08	25159151
707	Phosphorylation	KAAFQSQYKSHFVAA HHHHHHHHHHHHHHH	21.50	-
708	Methylation	AAFQSQYKSHFVAAS HHHHHHHHHHHHHHH	29.80	24129315
715	O-linked_Glycosylation	KSHFVAASLSNQKAG HHHHHHHHHCCCCCC	23.69	30059200
715	Phosphorylation	KSHFVAASLSNQKAG HHHHHHHHHCCCCCC	23.69	28450419
717	Phosphorylation	HFVAASLSNQKAGSS HHHHHHHCCCCCCCC	34.42	28450419
723	Phosphorylation	LSNQKAGSSAAGASG HCCCCCCCCCCCCCC	22.97	23927012
724	Phosphorylation	SNQKAGSSAAGASGW CCCCCCCCCCCCCCC	22.63	23927012
729	Phosphorylation	GSSAAGASGWTSAGS CCCCCCCCCCCCCCC	33.67	23927012
732	Phosphorylation	AAGASGWTSAGSLNS CCCCCCCCCCCCCCC	16.54	23927012
733	Phosphorylation	AGASGWTSAGSLNSV CCCCCCCCCCCCCCC	24.91	23927012
736	Phosphorylation	SGWTSAGSLNSVPTN CCCCCCCCCCCCCCC	24.88	23927012
739	Phosphorylation	TSAGSLNSVPTNSAQ CCCCCCCCCCCCCCC	34.46	23927012
742	Phosphorylation	GSLNSVPTNSAQQGH CCCCCCCCCCCCCCC	39.38	23401153
744	Phosphorylation	LNSVPTNSAQQGHNS CCCCCCCCCCCCCCC	29.90	30278072
751	Phosphorylation	SAQQGHNSPDSPVTS CCCCCCCCCCCCCCH	25.14	25159151
754	Phosphorylation	QGHNSPDSPVTSAAK CCCCCCCCCCCHHHC	25.34	25159151
757	Phosphorylation	NSPDSPVTSAAKGIP CCCCCCCCHHHCCCC	18.94	30278072
758	Phosphorylation	SPDSPVTSAAKGIPG CCCCCCCHHHCCCCC	26.85	30278072
773	Phosphorylation	FGNTGNISGAPVTYP CCCCCCCCCCCCCCC	32.66	28555341
792	Phosphorylation	QGVNNTASGNNSREG CCCCCCCCCCCCCCC	39.76	28555341
806	Acetylation	GTGGSNGKRERYTEN CCCCCCCCHHCCCCC	56.17	15615005
810	Phosphorylation	SNGKRERYTENRGSS CCCCHHCCCCCCCCC	17.41	21888424
814	Methylation	RERYTENRGSSRHSH HHCCCCCCCCCCCCC	39.89	-
817	Phosphorylation	YTENRGSSRHSHGET CCCCCCCCCCCCCCC	36.52	21888424
820	Phosphorylation	NRGSSRHSHGETGNR CCCCCCCCCCCCCCC	31.88	-
829	Phosphorylation	GETGNRHSDSPRHGD CCCCCCCCCCCCCCC	36.22	26699800
831	Phosphorylation	TGNRHSDSPRHGDGG CCCCCCCCCCCCCCC	27.18	30576142
844	Phosphorylation	GGRHGDGYRHPESSS CCCCCCCCCCCCCCC	15.53	-
850	Phosphorylation	GYRHPESSSRHTDGH CCCCCCCCCCCCCCC	29.72	30576142
851	Phosphorylation	YRHPESSSRHTDGHR CCCCCCCCCCCCCCC	37.04	30576142
875	Methylation	AGRHGENRGANDGRN CCCCCCCCCCCCCCC	41.10	-
894	Phosphorylation	KEAFNRESKMEPKME HHHHHHHHCCCCCCC	33.78	23917254
899	Sumoylation	RESKMEPKMEPKVDS HHHCCCCCCCCCCCH	42.46	-
899	Sumoylation	RESKMEPKMEPKVDS HHHCCCCCCCCCCCH	42.46	28112733
906	Phosphorylation	KMEPKVDSSKMDKVD CCCCCCCHHHHCCCC	34.54	28258704
907	Phosphorylation	MEPKVDSSKMDKVDS CCCCCCHHHHCCCCC	27.77	28258704
914	Phosphorylation	SKMDKVDSKTDKTAD HHHCCCCCCCCCCCC	40.20	-
918	Acetylation	KVDSKTDKTADGFAV CCCCCCCCCCCCCCC	51.34	26051181
938	Acetylation	RKKSRWDS------- CCCCCCCC-------	34.85	19608861
938	Phosphorylation	RKKSRWDS------- CCCCCCCC-------	34.85	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of DDX42_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of DDX42_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of DDX42_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
A4_HUMAN	APP	physical	21832049
TCRG1_HUMAN	TCERG1	physical	22939629
HNRPQ_HUMAN	SYNCRIP	physical	22939629
KS6A4_HUMAN	RPS6KA4	physical	22939629
RL36_HUMAN	RPL36	physical	22939629
PIN4_HUMAN	PIN4	physical	22939629
PHF20_HUMAN	PHF20	physical	22939629
KALM_HUMAN	KAL1	physical	22939629
NOSIP_HUMAN	NOSIP	physical	22939629
SYEP_HUMAN	EPRS	physical	22939629
FOXJ3_HUMAN	FOXJ3	physical	22939629
RU1C_HUMAN	SNRPC	physical	22365833
SF3A1_HUMAN	SF3A1	physical	22365833
SF3B4_HUMAN	SF3B4	physical	22365833
DHX15_HUMAN	DHX15	physical	22365833
SR140_HUMAN	U2SURP	physical	22365833
PRP19_HUMAN	PRPF19	physical	22365833

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1 AND LYS-5, AND MASSSPECTROMETRY.
PubMed: 19608861

Phosphorylation

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109 AND SER-111, ANDMASS SPECTROMETRY.
PubMed: 19690332

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185; SER-751 ANDSER-754, AND MASS SPECTROMETRY.
PubMed: 19413330

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-109; SER-111;SER-185 AND SER-754, AND MASS SPECTROMETRY.
PubMed: 18669648

"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-10; SER-96; SER-109 ANDSER-111, AND MASS SPECTROMETRY.
PubMed: 17081983