NIT1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NIT1_HUMAN
• UniProt AC: Q86X76
• Protein Name: Deaminated glutathione amidase
• Gene Name: NIT1
• Organism: Homo sapiens (Human).
• Sequence Length: 327
• Subcellular Localization: Isoform 2: Mitochondrion .; Isoform 1: Cytoplasm .
• Protein Description: Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione. Plays a role in cell growth and apoptosis: loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells..
• Protein Sequence: MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
40	Phosphorylation	RPRAMAISSSSCELP CCCEEEECCCCCCCC	18.03	25693802
41	Phosphorylation	PRAMAISSSSCELPL CCEEEECCCCCCCCE	21.84	25693802
42	Phosphorylation	RAMAISSSSCELPLV CEEEECCCCCCCCEE	32.02	25693802
43	Phosphorylation	AMAISSSSCELPLVA EEEECCCCCCCCEEE	17.35	25693802
65	Ubiquitination	PDKQQNFKTCAELVR CCHHHCHHHHHHHHH	50.69	-
72 (in isoform 2)	Ubiquitination	-	40.22	21906983
77 (in isoform 3)	Ubiquitination	-	4.35	-
93 (in isoform 4)	Ubiquitination	-	45.70	21906983
102	Phosphorylation	PAETLHLSEPLGGKL HHHHCCCCCCCCHHH	26.81	20071362
108	Ubiquitination	LSEPLGGKLLEEYTQ CCCCCCHHHHHHHHH	48.37	2190698
108 (in isoform 1)	Ubiquitination	-	48.37	21906983
125 (in isoform 3)	Ubiquitination	-	17.24	21906983
141	Ubiquitination	QDWEQTQKIYNCHVL CCHHHHHHHHEEEEE	51.01	-
143	Phosphorylation	WEQTQKIYNCHVLLN HHHHHHHHEEEEEEC	20.83	22817900
151	Phosphorylation	NCHVLLNSKGAVVAT EEEEEECCCCEEEEE	32.22	28634298
152	Acetylation	CHVLLNSKGAVVATY EEEEECCCCEEEEEE	50.22	30590753
158 (in isoform 3)	Ubiquitination	-	16.55	-
158	Phosphorylation	SKGAVVATYRKTHLC CCCEEEEEEEECCCC	16.55	21406692
159	Phosphorylation	KGAVVATYRKTHLCD CCEEEEEEEECCCCC	10.60	25072903
178	Phosphorylation	GQGPMCESNSTMPGP CCCCCCCCCCCCCCC	31.02	-
180	Phosphorylation	GPMCESNSTMPGPSL CCCCCCCCCCCCCCC	35.05	-
268	Phosphorylation	RHHEKRASYGHSMVV CCCCHHHHCCCCEEE	35.36	28857561
269	Phosphorylation	HHEKRASYGHSMVVD CCCHHHHCCCCEEEC	20.20	28857561
272	Phosphorylation	KRASYGHSMVVDPWG HHHHCCCCEEECCCC	14.65	28857561
319	Phosphorylation	QHRRPDLYGNLGHPL CCCCCCCCCCCCCCC	16.02	27642862
327	Phosphorylation	GNLGHPLS------- CCCCCCCC-------	42.50	26091039

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NIT1_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NIT1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NIT1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
XRCC6_HUMAN	XRCC6	physical	16169070
CUX1_HUMAN	CUX1	physical	26344197
CASP_HUMAN	CUX1	physical	26344197
PSMG4_HUMAN	PSMG4	physical	26344197
NIT1_HUMAN	NIT1	physical	27499296
UCP4_HUMAN	SLC25A27	physical	27499296
CAF17_HUMAN	IBA57	physical	27499296
MPPB_HUMAN	PMPCB	physical	27499296
MPPA_HUMAN	PMPCA	physical	27499296
HOGA1_HUMAN	HOGA1	physical	27499296
NUDT8_HUMAN	NUDT8	physical	27499296
BOLA3_HUMAN	BOLA3	physical	27499296
PREP_HUMAN	PITRM1	physical	27499296
ECH1_HUMAN	ECH1	physical	27499296
PDIP2_HUMAN	POLDIP2	physical	27499296
C1QBP_HUMAN	C1QBP	physical	27499296
IDE_HUMAN	IDE	physical	27499296
GTPB8_HUMAN	GTPBP8	physical	28514442
MRM2_HUMAN	FTSJ2	physical	28514442
DPOG1_HUMAN	POLG	physical	28514442
ACSF2_HUMAN	ACSF2	physical	28514442
SYLM_HUMAN	LARS2	physical	28514442
MCAT_HUMAN	SLC25A20	physical	28514442
NPS3A_HUMAN	NIPSNAP3A	physical	28514442
ADRO_HUMAN	FDXR	physical	28514442
MRRP3_HUMAN	KIAA0391	physical	28514442
NUD19_HUMAN	NUDT19	physical	28514442
SYNM_HUMAN	NARS2	physical	28514442
RUSD3_HUMAN	RPUSD3	physical	28514442
FOLC_HUMAN	FPGS	physical	28514442
TOP3A_HUMAN	TOP3A	physical	28514442
BCKD_HUMAN	BCKDK	physical	28514442
SIR3_HUMAN	SIRT3	physical	28514442
HEMH_HUMAN	FECH	physical	28514442
TAZ_HUMAN	TAZ	physical	28514442
BCAT2_HUMAN	BCAT2	physical	28514442
CQ080_HUMAN	C17orf80	physical	28514442
PDPR_HUMAN	PDPR	physical	28514442
TBA4A_HUMAN	TUBA4A	physical	28514442
CLPX_HUMAN	CLPX	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.