dbPTM

AFF4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AFF4_HUMAN
UniProt AC	Q9UHB7
Protein Name	AF4/FMR2 family member 4
Gene Name	AFF4
Organism	Homo sapiens (Human).
Sequence Length	1163
Subcellular Localization	Nucleus . Associates to transcriptionally active chromatin but not at snRNA genes..
Protein Description	Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression..
Protein Sequence	MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
31	Phosphorylation	GEDAFPPSSPLFAEP CCCCCCCCCCCCCCC	44.84	30266825
32	Phosphorylation	EDAFPPSSPLFAEPY CCCCCCCCCCCCCCE	31.25	30266825
39	Phosphorylation	SPLFAEPYKVTSKED CCCCCCCEECCCHHH	15.03	30576142
42	O-linked_Glycosylation	FAEPYKVTSKEDKLS CCCCEECCCHHHHHH	29.90	30059200
42	Phosphorylation	FAEPYKVTSKEDKLS CCCCEECCCHHHHHH	29.90	26074081
43	O-linked_Glycosylation	AEPYKVTSKEDKLSS CCCEECCCHHHHHHH	36.71	30059200
43	Phosphorylation	AEPYKVTSKEDKLSS CCCEECCCHHHHHHH	36.71	26074081
49	Phosphorylation	TSKEDKLSSRIQSML CCHHHHHHHHHHHHH	24.26	29978859
50	Phosphorylation	SKEDKLSSRIQSMLG CHHHHHHHHHHHHHC	43.79	29978859
54	Phosphorylation	KLSSRIQSMLGNYDE HHHHHHHHHHCCHHH	17.38	29978859
59	Phosphorylation	IQSMLGNYDEMKDFI HHHHHCCHHHHHHHH	16.10	27642862
63	Ubiquitination	LGNYDEMKDFIGDRS HCCHHHHHHHHCCCC	47.65	23503661
70	Phosphorylation	KDFIGDRSIPKLVAI HHHHCCCCCCEEEEC	48.24	28555341
79	Acetylation	PKLVAIPKPTVPPSA CEEEECCCCCCCCCC	47.40	19608861
89	Acetylation	VPPSADEKSNPNFFE CCCCCCCCCCCCHHH	56.96	26051181
90	Phosphorylation	PPSADEKSNPNFFEQ CCCCCCCCCCCHHHH	56.31	25159151
107	Acetylation	GGSHQSSKWTPVGPA CCCCCCCCCCCCCCC	61.40	25953088
107	Ubiquitination	GGSHQSSKWTPVGPA CCCCCCCCCCCCCCC	61.40	21963094
109	Phosphorylation	SHQSSKWTPVGPAPS CCCCCCCCCCCCCCC	16.31	28985074
116	Phosphorylation	TPVGPAPSTSQSQKR CCCCCCCCCCHHHHH	42.68	25159151
117	Phosphorylation	PVGPAPSTSQSQKRS CCCCCCCCCHHHHHH	29.71	25159151
118	Phosphorylation	VGPAPSTSQSQKRSS CCCCCCCCHHHHHHC	31.97	25159151
120	Phosphorylation	PAPSTSQSQKRSSGL CCCCCCHHHHHHCCC	37.12	25159151
122	Acetylation	PSTSQSQKRSSGLQS CCCCHHHHHHCCCCC	60.54	25953088
124	Phosphorylation	TSQSQKRSSGLQSGH CCHHHHHHCCCCCCC	35.47	17192257
125	Phosphorylation	SQSQKRSSGLQSGHS CHHHHHHCCCCCCCC	47.78	28348404
132	Phosphorylation	SGLQSGHSSQRTSAG CCCCCCCCCCCCCCC	31.52	24719451
133	Phosphorylation	GLQSGHSSQRTSAGS CCCCCCCCCCCCCCC	20.29	21712546
136	Phosphorylation	SGHSSQRTSAGSSSG CCCCCCCCCCCCCCC	18.45	21712546
144	Ubiquitination	SAGSSSGTNSSGQRH CCCCCCCCCCCCCCC	33.60	24816145
155	Phosphorylation	GQRHDRESYNNSGSS CCCCCHHHCCCCCCC	33.77	-
156	Phosphorylation	QRHDRESYNNSGSSS CCCCHHHCCCCCCCC	17.38	23532336
159	Phosphorylation	DRESYNNSGSSSRKK CHHHCCCCCCCCCCC	36.09	23532336
161	Phosphorylation	ESYNNSGSSSRKKGQ HHCCCCCCCCCCCCC	25.59	29449344
162	Phosphorylation	SYNNSGSSSRKKGQH HCCCCCCCCCCCCCC	37.37	29449344
163	Phosphorylation	YNNSGSSSRKKGQHG CCCCCCCCCCCCCCC	50.22	29449344
166	Acetylation	SGSSSRKKGQHGSEH CCCCCCCCCCCCCCC	63.79	7667137
176	Phosphorylation	HGSEHSKSRSSSPGK CCCCCCCCCCCCCCC	40.17	25159151
178	Phosphorylation	SEHSKSRSSSPGKPQ CCCCCCCCCCCCCCC	42.11	25159151
179	Phosphorylation	EHSKSRSSSPGKPQA CCCCCCCCCCCCCCH	39.17	20201521
180	Phosphorylation	HSKSRSSSPGKPQAV CCCCCCCCCCCCCHH	38.63	25159151
188	Phosphorylation	PGKPQAVSSLNSSHS CCCCCHHHHCCCCCC	31.93	23927012
189	Phosphorylation	GKPQAVSSLNSSHSR CCCCHHHHCCCCCCC	25.60	23927012
191	Ubiquitination	PQAVSSLNSSHSRSH CCHHHHCCCCCCCCC	43.33	24816145
192	Phosphorylation	QAVSSLNSSHSRSHG CHHHHCCCCCCCCCC	34.42	23927012
193	Phosphorylation	AVSSLNSSHSRSHGN HHHHCCCCCCCCCCC	25.14	25159151
195	Phosphorylation	SSLNSSHSRSHGNDH HHCCCCCCCCCCCCC	37.11	25159151
205	Ubiquitination	HGNDHHSKEHQRSKS CCCCCCCHHHHHCCC	55.66	21963094
210	Phosphorylation	HSKEHQRSKSPRDPD CCHHHHHCCCCCCCC	30.80	23927012
212	Phosphorylation	KEHQRSKSPRDPDAN HHHHHCCCCCCCCCC	26.81	23401153
222	Phosphorylation	DPDANWDSPSRVPFS CCCCCCCCCCCCCCC	19.18	23927012
224	Phosphorylation	DANWDSPSRVPFSSG CCCCCCCCCCCCCCC	50.27	23927012
229	O-linked_Glycosylation	SPSRVPFSSGQHSTQ CCCCCCCCCCCCCCC	27.73	30059200
252	Acetylation	KSNSMLQKPTAYVRP CCCCCCCCCCEEECC	40.13	23954790
252	Ubiquitination	KSNSMLQKPTAYVRP CCCCCCCCCCEEECC	40.13	29967540
265	Phosphorylation	RPMDGQESMEPKLSS CCCCCCCCCCCCCCC	22.13	25159151
271	Phosphorylation	ESMEPKLSSEHYSSQ CCCCCCCCCCCCCCC	39.54	29978859
272	Phosphorylation	SMEPKLSSEHYSSQS CCCCCCCCCCCCCCC	38.54	29978859
275	Phosphorylation	PKLSSEHYSSQSHGN CCCCCCCCCCCCCCC	13.13	29978859
276	Phosphorylation	KLSSEHYSSQSHGNS CCCCCCCCCCCCCCC	24.65	29978859
277	Phosphorylation	LSSEHYSSQSHGNSM CCCCCCCCCCCCCCC	28.65	29978859
279	Phosphorylation	SEHYSSQSHGNSMTE CCCCCCCCCCCCCCC	35.00	29978859
283	Phosphorylation	SSQSHGNSMTELKPS CCCCCCCCCCCCCCC	31.51	29978859
285	Phosphorylation	QSHGNSMTELKPSSK CCCCCCCCCCCCCCC	37.68	29978859
288	Acetylation	GNSMTELKPSSKAHL CCCCCCCCCCCCCCC	36.30	26051181
288	Ubiquitination	GNSMTELKPSSKAHL CCCCCCCCCCCCCCC	36.30	27667366
288 (in isoform 1)	Ubiquitination	-	36.30	21906983
288 (in isoform 2)	Ubiquitination	-	36.30	21906983
288 (in isoform 3)	Ubiquitination	-	36.30	21906983
290	Phosphorylation	SMTELKPSSKAHLTK CCCCCCCCCCCCCCC	42.77	29978859
291	Phosphorylation	MTELKPSSKAHLTKL CCCCCCCCCCCCCCC	42.25	29978859
292	Ubiquitination	TELKPSSKAHLTKLK CCCCCCCCCCCCCCC	44.13	21963094
296	Phosphorylation	PSSKAHLTKLKIPSQ CCCCCCCCCCCCCCC	25.19	29978859
297	Ubiquitination	SSKAHLTKLKIPSQP CCCCCCCCCCCCCCC	54.89	21963094
299	Ubiquitination	KAHLTKLKIPSQPLD CCCCCCCCCCCCCCC	54.87	21963094
302	Phosphorylation	LTKLKIPSQPLDASA CCCCCCCCCCCCCCC	48.15	-
308	Phosphorylation	PSQPLDASASGDVSC CCCCCCCCCCCCCHH	23.72	25159151
310	Phosphorylation	QPLDASASGDVSCVD CCCCCCCCCCCHHHH	32.79	25159151
314	Phosphorylation	ASASGDVSCVDEILK CCCCCCCHHHHHHHH	17.11	25159151
321	Ubiquitination	SCVDEILKEMTHSWP HHHHHHHHHHHHCCC	51.61	21963094
324	Phosphorylation	DEILKEMTHSWPPPL HHHHHHHHHCCCCCC	17.53	28634298
326	Phosphorylation	ILKEMTHSWPPPLTA HHHHHHHCCCCCCEE	31.92	28634298
332	Phosphorylation	HSWPPPLTAIHTPCK HCCCCCCEEEECCCC	29.37	30576142
334	Ubiquitination	WPPPLTAIHTPCKTE CCCCCEEEECCCCCC	2.91	23503661
336	Phosphorylation	PPLTAIHTPCKTEPS CCCEEEECCCCCCCC	25.03	25159151
339	Ubiquitination	TAIHTPCKTEPSKFP EEEECCCCCCCCCCC	58.06	23000965
340	Phosphorylation	AIHTPCKTEPSKFPF EEECCCCCCCCCCCC	60.90	28634298
340	Ubiquitination	AIHTPCKTEPSKFPF EEECCCCCCCCCCCC	60.90	23503661
343	Phosphorylation	TPCKTEPSKFPFPTK CCCCCCCCCCCCCCH	40.47	28634298
344	Acetylation	PCKTEPSKFPFPTKE CCCCCCCCCCCCCHH	68.70	25953088
344	Ubiquitination	PCKTEPSKFPFPTKE CCCCCCCCCCCCCHH	68.70	23000965
349	Phosphorylation	PSKFPFPTKESQQSN CCCCCCCCHHHHCCC	48.07	29083192
350	Acetylation	SKFPFPTKESQQSNF CCCCCCCHHHHCCCC	56.45	26051181
350	Ubiquitination	SKFPFPTKESQQSNF CCCCCCCHHHHCCCC	56.45	23000965
355	Phosphorylation	PTKESQQSNFGTGEQ CCHHHHCCCCCCCCC	26.87	29396449
359	Phosphorylation	SQQSNFGTGEQKRYN HHCCCCCCCCCCCCC	33.15	21815630
363	Ubiquitination	NFGTGEQKRYNPSKT CCCCCCCCCCCCCCC	53.18	32015554
364	Ubiquitination	FGTGEQKRYNPSKTS CCCCCCCCCCCCCCC	35.83	24816145
369	Ubiquitination	QKRYNPSKTSNGHQS CCCCCCCCCCCCCCC	58.17	23503661
371	Phosphorylation	RYNPSKTSNGHQSKS CCCCCCCCCCCCCHH	44.55	-
377	Acetylation	TSNGHQSKSMLKDDL CCCCCCCHHHHHHHH	33.06	25953088
387	Phosphorylation	LKDDLKLSSSEDSDG HHHHHCCCCCCCCCC	30.17	23927012
388	Phosphorylation	KDDLKLSSSEDSDGE HHHHCCCCCCCCCCC	48.46	23927012
389	Phosphorylation	DDLKLSSSEDSDGEQ HHHCCCCCCCCCCCC	41.89	23927012
392	Phosphorylation	KLSSSEDSDGEQDCD CCCCCCCCCCCCCCC	43.39	23927012
405	Phosphorylation	CDKTMPRSTPGSNSE CCCCCCCCCCCCCCC	32.84	25849741
406	Phosphorylation	DKTMPRSTPGSNSEP CCCCCCCCCCCCCCC	32.71	25849741
409	Phosphorylation	MPRSTPGSNSEPSHH CCCCCCCCCCCCCCC	38.08	25849741
411	Phosphorylation	RSTPGSNSEPSHHNS CCCCCCCCCCCCCCC	53.21	23312004
414	Phosphorylation	PGSNSEPSHHNSEGA CCCCCCCCCCCCCCC	33.07	23312004
418	Phosphorylation	SEPSHHNSEGADNSR CCCCCCCCCCCCCCC	32.81	23312004
424	Phosphorylation	NSEGADNSRDDSSSH CCCCCCCCCCCCCCC	37.32	24532841
440	Phosphorylation	GSESSSGSDSESESS CCCCCCCCCCCCCCC	39.65	-
487	Phosphorylation	KVNPHKVSPASSVDS HCCCCCCCCCHHCCC	21.39	25159151
490	Phosphorylation	PHKVSPASSVDSNIP CCCCCCCHHCCCCCC	33.99	23927012
491	Phosphorylation	HKVSPASSVDSNIPS CCCCCCHHCCCCCCC	31.86	23927012
493	Ubiquitination	VSPASSVDSNIPSSQ CCCCHHCCCCCCCCC	37.60	24816145
494	Phosphorylation	SPASSVDSNIPSSQG CCCHHCCCCCCCCCC	34.21	30266825
498	Phosphorylation	SVDSNIPSSQGYKKE HCCCCCCCCCCCCCC	31.34	30266825
499	Phosphorylation	VDSNIPSSQGYKKEG CCCCCCCCCCCCCCC	23.44	17525332
502	Phosphorylation	NIPSSQGYKKEGREQ CCCCCCCCCCCCCCC	15.96	23927012
503	Acetylation	IPSSQGYKKEGREQG CCCCCCCCCCCCCCC	51.69	23236377
504	Acetylation	PSSQGYKKEGREQGT CCCCCCCCCCCCCCC	57.84	30590337
511	Phosphorylation	KEGREQGTGNSYTDT CCCCCCCCCCCCCCC	32.65	25159151
514	Phosphorylation	REQGTGNSYTDTSGP CCCCCCCCCCCCCCC	30.65	25159151
515	Phosphorylation	EQGTGNSYTDTSGPK CCCCCCCCCCCCCCC	16.79	23312004
516	Phosphorylation	QGTGNSYTDTSGPKE CCCCCCCCCCCCCCC	32.40	25159151
518	Phosphorylation	TGNSYTDTSGPKETS CCCCCCCCCCCCCCC	27.99	23312004
519	Phosphorylation	GNSYTDTSGPKETSS CCCCCCCCCCCCCCC	56.77	21815630
522	Ubiquitination	YTDTSGPKETSSATP CCCCCCCCCCCCCCC	76.72	24816145
524	Phosphorylation	DTSGPKETSSATPGR CCCCCCCCCCCCCCC	33.82	29396449
525	Phosphorylation	TSGPKETSSATPGRD CCCCCCCCCCCCCCC	21.04	25849741
526	Phosphorylation	SGPKETSSATPGRDS CCCCCCCCCCCCCCC	43.51	25849741
528	Phosphorylation	PKETSSATPGRDSKT CCCCCCCCCCCCCCC	28.37	23401153
533	Phosphorylation	SATPGRDSKTIQKGS CCCCCCCCCCCCCCC	30.27	28985074
540	Phosphorylation	SKTIQKGSESGRGRQ CCCCCCCCCCCCCCC	35.31	28348404
540	Ubiquitination	SKTIQKGSESGRGRQ CCCCCCCCCCCCCCC	35.31	24816145
549	Phosphorylation	SGRGRQKSPAQSDST CCCCCCCCCCCCCCC	19.68	30278072
553	Phosphorylation	RQKSPAQSDSTTQRR CCCCCCCCCCCHHHH	35.32	30278072
554	Ubiquitination	QKSPAQSDSTTQRRT CCCCCCCCCCHHHHC	37.61	21963094
555	Phosphorylation	KSPAQSDSTTQRRTV CCCCCCCCCHHHHCC	38.30	24732914
556	Phosphorylation	SPAQSDSTTQRRTVG CCCCCCCCHHHHCCC	31.75	24732914
557	Phosphorylation	PAQSDSTTQRRTVGK CCCCCCCHHHHCCCC	24.83	24732914
564	Acetylation	TQRRTVGKKQPKKAE HHHHCCCCCCCHHHH	43.85	26051181
565	Acetylation	QRRTVGKKQPKKAEK HHHCCCCCCCHHHHH	66.94	26051181
569	Acetylation	VGKKQPKKAEKAAAE CCCCCCHHHHHHHHH	69.34	25953088
569	Ubiquitination	VGKKQPKKAEKAAAE CCCCCCHHHHHHHHH	69.34	24816145
572	Acetylation	KQPKKAEKAAAEEPR CCCHHHHHHHHHCCC	48.46	25953088
580	Ubiquitination	AAAEEPRGGLKIESE HHHHCCCCCCEEEEC	57.40	23503661
583	Sumoylation	EEPRGGLKIESETPV HCCCCCCEEEECCCC	47.94	-
583	Sumoylation	EEPRGGLKIESETPV HCCCCCCEEEECCCC	47.94	28112733
583	Ubiquitination	EEPRGGLKIESETPV HCCCCCCEEEECCCC	47.94	21963094
586	Ubiquitination	RGGLKIESETPVDLA CCCCEEEECCCCCHH	50.69	23503661
588	Phosphorylation	GLKIESETPVDLASS CCEEEECCCCCHHHC	37.58	29396449
594	Phosphorylation	ETPVDLASSMPSSRH CCCCCHHHCCCCHHH	34.17	25159151
595	Phosphorylation	TPVDLASSMPSSRHK CCCCHHHCCCCHHHH	29.16	20068231
598	Phosphorylation	DLASSMPSSRHKAAT CHHHCCCCHHHHHHH	31.22	20068231
599	Phosphorylation	LASSMPSSRHKAATK HHHCCCCHHHHHHHC	32.31	25159151
617	Phosphorylation	KPNIKKESKSSPRPT CCCCCCCCCCCCCCC	46.87	23312004
618	Ubiquitination	PNIKKESKSSPRPTA CCCCCCCCCCCCCCH	56.92	23503661
619	Phosphorylation	NIKKESKSSPRPTAE CCCCCCCCCCCCCHH	55.65	26657352
620	Phosphorylation	IKKESKSSPRPTAEK CCCCCCCCCCCCHHH	28.76	29396449
624	Phosphorylation	SKSSPRPTAEKKKYK CCCCCCCCHHHHHHC	49.05	29743597
662	Phosphorylation	ESESLPPSSQTPKYP CCCCCCCHHCCCCCC	32.89	30576142
665	Phosphorylation	SLPPSSQTPKYPESN CCCCHHCCCCCCCCC	24.56	30576142
668	Phosphorylation	PSSQTPKYPESNRTP CHHCCCCCCCCCCCC	17.28	23927012
671	Phosphorylation	QTPKYPESNRTPVKP CCCCCCCCCCCCCCC	27.40	23927012
674	Phosphorylation	KYPESNRTPVKPSSV CCCCCCCCCCCCCCH	36.36	29255136
677	Acetylation	ESNRTPVKPSSVEEE CCCCCCCCCCCHHHC	40.15	26051181
677	Ubiquitination	ESNRTPVKPSSVEEE CCCCCCCCCCCHHHC	40.15	29967540
679	Phosphorylation	NRTPVKPSSVEEEDS CCCCCCCCCHHHCCH	41.10	30266825
680	Phosphorylation	RTPVKPSSVEEEDSF CCCCCCCCHHHCCHH	41.92	30266825
686	Phosphorylation	SSVEEEDSFFRQRMF CCHHHCCHHHHHHHC	29.57	30266825
694	Phosphorylation	FFRQRMFSPMEEKEL HHHHHHCCCHHHHHH	17.62	29255136
703	Phosphorylation	MEEKELLSPLSEPDD HHHHHHHCCCCCCCC	36.10	29255136
706	Phosphorylation	KELLSPLSEPDDRYP HHHHCCCCCCCCCCC	50.93	29255136
712	Phosphorylation	LSEPDDRYPLIVKID CCCCCCCCCEEEEEE	14.91	23927012
713	Ubiquitination	SEPDDRYPLIVKIDL CCCCCCCCEEEEEEH	19.26	24816145
724	Phosphorylation	KIDLNLLTRIPGKPY EEEHHHHHCCCCCCC	29.71	24117733
742	Ubiquitination	EPPKGEKKNVPEKHT CCCCCCCCCCCHHHH	59.90	24816145
747	Acetylation	EKKNVPEKHTREAQK CCCCCCHHHHHHHHH	44.18	23749302
757	Phosphorylation	REAQKQASEKVSNKG HHHHHHHHHHHHHHH	35.14	29052541
761	Phosphorylation	KQASEKVSNKGKRKH HHHHHHHHHHHHHCC	43.84	29052541
784	Phosphorylation	SESKKPKTEDKNSAG CCCCCCCCCCCCCCC	58.92	23312004
789	Phosphorylation	PKTEDKNSAGHKPSS CCCCCCCCCCCCCCC	41.17	23312004
795	Phosphorylation	NSAGHKPSSNRESSK CCCCCCCCCCHHHHH	45.08	23312004
796	Phosphorylation	SAGHKPSSNRESSKQ CCCCCCCCCHHHHHH	48.73	23312004
800	Phosphorylation	KPSSNRESSKQSAAK CCCCCHHHHHHHHHH	39.33	23312004
801	Phosphorylation	PSSNRESSKQSAAKE CCCCHHHHHHHHHHH	29.93	23312004
804	Phosphorylation	NRESSKQSAAKEKDL CHHHHHHHHHHHHCC	33.65	-
814	O-linked_Glycosylation	KEKDLLPSPAGPVPS HHHCCCCCCCCCCCC	27.61	30059200
814	Phosphorylation	KEKDLLPSPAGPVPS HHHCCCCCCCCCCCC	27.61	29255136
821	Phosphorylation	SPAGPVPSKDPKTEH CCCCCCCCCCCCCCC	50.34	23927012
822	Acetylation	PAGPVPSKDPKTEHG CCCCCCCCCCCCCCC	72.48	23954790
834	Phosphorylation	EHGSRKRTISQSSSL CCCCCCCEECCHHHC	28.19	30266825
836	Phosphorylation	GSRKRTISQSSSLKS CCCCCEECCHHHCCC	24.32	25159151
838	Phosphorylation	RKRTISQSSSLKSSS CCCEECCHHHCCCCC	18.33	30266825
839	Phosphorylation	KRTISQSSSLKSSSN CCEECCHHHCCCCCC	31.86	30266825
840	Phosphorylation	RTISQSSSLKSSSNS CEECCHHHCCCCCCC	45.09	30266825
843	Phosphorylation	SQSSSLKSSSNSNKE CCHHHCCCCCCCCCC	44.27	29052541
844	Phosphorylation	QSSSLKSSSNSNKET CHHHCCCCCCCCCCC	31.77	29052541
845	Phosphorylation	SSSLKSSSNSNKETS HHHCCCCCCCCCCCC	51.90	22210691
847	Phosphorylation	SLKSSSNSNKETSGS HCCCCCCCCCCCCCC	51.75	29052541
849	Acetylation	KSSSNSNKETSGSSK CCCCCCCCCCCCCCC	63.29	30590343
851	O-linked_Glycosylation	SSNSNKETSGSSKNS CCCCCCCCCCCCCCC	39.74	30059200
851	Phosphorylation	SSNSNKETSGSSKNS CCCCCCCCCCCCCCC	39.74	29978859
852	Phosphorylation	SNSNKETSGSSKNSS CCCCCCCCCCCCCCC	37.46	29978859
854	O-linked_Glycosylation	SNKETSGSSKNSSST CCCCCCCCCCCCCCC	37.60	30059200
854	Phosphorylation	SNKETSGSSKNSSST CCCCCCCCCCCCCCC	37.60	29978859
855	O-linked_Glycosylation	NKETSGSSKNSSSTS CCCCCCCCCCCCCCC	39.11	30059200
855	Phosphorylation	NKETSGSSKNSSSTS CCCCCCCCCCCCCCC	39.11	29978859
858	Phosphorylation	TSGSSKNSSSTSKQK CCCCCCCCCCCCCCH	29.42	24505115
859	Phosphorylation	SGSSKNSSSTSKQKK CCCCCCCCCCCCCHH	47.21	17929957
860	Phosphorylation	GSSKNSSSTSKQKKT CCCCCCCCCCCCHHC	36.20	29978859
861	Phosphorylation	SSKNSSSTSKQKKTE CCCCCCCCCCCHHCC	41.72	17929957
862	Phosphorylation	SKNSSSTSKQKKTEG CCCCCCCCCCHHCCC	34.73	24505115
867	Phosphorylation	STSKQKKTEGKTSSS CCCCCHHCCCCCCCC	57.94	21712546
871	O-linked_Glycosylation	QKKTEGKTSSSSKEV CHHCCCCCCCCCHHH	44.70	30059200
871	Phosphorylation	QKKTEGKTSSSSKEV CHHCCCCCCCCCHHH	44.70	21712546
872	Phosphorylation	KKTEGKTSSSSKEVK HHCCCCCCCCCHHHH	31.54	-
873	Phosphorylation	KTEGKTSSSSKEVKE HCCCCCCCCCHHHHH	43.81	-
874	Phosphorylation	TEGKTSSSSKEVKEK CCCCCCCCCHHHHHH	45.24	-
875	Phosphorylation	EGKTSSSSKEVKEKA CCCCCCCCHHHHHHC	34.48	25137130
881 (in isoform 2)	Ubiquitination	-	60.94	-
885	Phosphorylation	VKEKAPSSSSNCPPS HHHHCCCCCCCCCCC	36.41	28985074
898	Phosphorylation	PSAPTLDSSKPRRTK CCCCCCCCCCCCCEE	43.29	28985074
900	Acetylation	APTLDSSKPRRTKLV CCCCCCCCCCCEEEE	46.63	26051181
905	Acetylation	SSKPRRTKLVFDDRN CCCCCCEEEEECCCC	40.62	25953088
905	Ubiquitination	SSKPRRTKLVFDDRN CCCCCCEEEEECCCC	40.62	29967540
911	Methylation	TKLVFDDRNYSADHY EEEEECCCCCCHHHH	45.92	115482789
929	Ubiquitination	AKKLKHNADALSDRF HHHHCCCHHHHHHHH	11.81	23503661
935	Methylation	NADALSDRFEKAVYY CHHHHHHHHHHHHHH	37.94	115482781
935	Ubiquitination	NADALSDRFEKAVYY CHHHHHHHHHHHHHH	37.94	23503661
958	Ubiquitination	ECGNALEKNAQESKS HHHHHHHHHHHHCCC	59.44	23503661
963	Phosphorylation	LEKNAQESKSPFPMY HHHHHHHCCCCCCCC	26.76	-
964	Ubiquitination	EKNAQESKSPFPMYS HHHHHHCCCCCCCCH	62.49	23503661
965	Phosphorylation	KNAQESKSPFPMYSE HHHHHCCCCCCCCHH	40.84	29759185
967	Ubiquitination	AQESKSPFPMYSETV HHHCCCCCCCCHHHH	7.58	23503661
970	Phosphorylation	SKSPFPMYSETVDLI CCCCCCCCHHHHHHH	12.00	29759185
971	Phosphorylation	KSPFPMYSETVDLIK CCCCCCCHHHHHHHH	22.12	29759185
973	Phosphorylation	PFPMYSETVDLIKYT CCCCCHHHHHHHHHH	17.24	-
986	Phosphorylation	YTMKLKNYLAPDATA HHHHHHHHCCCCCCH	11.58	22461510
996	Ubiquitination	PDATAADKRLTVLCL CCCCHHHHHHHHHHH	44.58	23503661
999	Phosphorylation	TAADKRLTVLCLRCE CHHHHHHHHHHHHHH	18.57	-
1026	Ubiquitination	ENALKYSKTLTEHLK HHHHHHHHHHHHHHH	44.45	29967540
1035	Phosphorylation	LTEHLKNSYNNSQAP HHHHHHHHCCCCCCC	27.87	23663014
1036	Phosphorylation	TEHLKNSYNNSQAPS HHHHHHHCCCCCCCC	27.97	23663014
1039	Phosphorylation	LKNSYNNSQAPSPGL HHHHCCCCCCCCCCC	24.87	30266825
1043	Phosphorylation	YNNSQAPSPGLGSKA CCCCCCCCCCCCCCC	34.32	19664994
1048	Phosphorylation	APSPGLGSKAVGMPS CCCCCCCCCCCCCCC	23.60	30266825
1049	Acetylation	PSPGLGSKAVGMPSP CCCCCCCCCCCCCCC	45.34	26051181
1055	Phosphorylation	SKAVGMPSPVSPKLS CCCCCCCCCCCCCCC	29.10	29255136
1058	Phosphorylation	VGMPSPVSPKLSPGN CCCCCCCCCCCCCCC	21.50	29255136
1062	Phosphorylation	SPVSPKLSPGNSGNY CCCCCCCCCCCCCCC	36.57	30278072
1066	Phosphorylation	PKLSPGNSGNYSSGA CCCCCCCCCCCCCCC	34.04	30278072
1069	Phosphorylation	SPGNSGNYSSGASSA CCCCCCCCCCCCCCC	14.03	30278072
1070	Phosphorylation	PGNSGNYSSGASSAS CCCCCCCCCCCCCCC	26.71	23927012
1071	Phosphorylation	GNSGNYSSGASSASA CCCCCCCCCCCCCCC	28.19	23927012
1074	Phosphorylation	GNYSSGASSASASGS CCCCCCCCCCCCCCC	29.93	23927012
1075	Phosphorylation	NYSSGASSASASGSS CCCCCCCCCCCCCCC	26.21	23927012
1077	Phosphorylation	SSGASSASASGSSVT CCCCCCCCCCCCCCC	25.56	23403867
1079	Phosphorylation	GASSASASGSSVTIP CCCCCCCCCCCCCCC	36.39	23403867
1081	Phosphorylation	SSASASGSSVTIPQK CCCCCCCCCCCCCHH	21.09	23403867
1082	Phosphorylation	SASASGSSVTIPQKI CCCCCCCCCCCCHHH	27.14	23403867
1084	Phosphorylation	SASGSSVTIPQKIHQ CCCCCCCCCCHHHHH	28.96	23403867
1121	Ubiquitination	EQLSKEQKEFFAELD HHHCHHHHHHHHHHH	58.54	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of AFF4_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AFF4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AFF4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CDK9_HUMAN	CDK9	physical	12065898
CCNT1_HUMAN	CCNT1	physical	12065898
ENL_HUMAN	MLLT1	physical	20153263
CCNT1_HUMAN	CCNT1	physical	20153263
CDK9_HUMAN	CDK9	physical	20153263
AFF1_HUMAN	AFF1	physical	20153263
KMT2A_HUMAN	KMT2A	physical	20153263
ELL2_HUMAN	ELL2	physical	22483617
ENL_HUMAN	MLLT1	physical	21873227
AF9_HUMAN	MLLT3	physical	21873227
CDK9_HUMAN	CDK9	physical	21873227
ELL_HUMAN	ELL	physical	21729782
ELL2_HUMAN	ELL2	physical	21729782
EAF1_HUMAN	EAF1	physical	21729782
EAF2_HUMAN	EAF2	physical	21729782
AFF1_HUMAN	AFF1	physical	21729782
ENL_HUMAN	MLLT1	physical	21729782
AF9_HUMAN	MLLT3	physical	21729782
CDK9_HUMAN	CDK9	physical	21729782
CCNT1_HUMAN	CCNT1	physical	21729782
CCNT2_HUMAN	CCNT2	physical	21729782
CDK9_HUMAN	CDK9	physical	26186194
ELL2_HUMAN	ELL2	physical	26186194
EAF2_HUMAN	EAF2	physical	26186194
HEXI1_HUMAN	HEXIM1	physical	26186194
ELL3_HUMAN	ELL3	physical	26186194
CCNT2_HUMAN	CCNT2	physical	26186194
CCNT1_HUMAN	CCNT1	physical	26186194
ELL_HUMAN	ELL	physical	26186194
ENL_HUMAN	MLLT1	physical	26344197
ELL3_HUMAN	ELL3	physical	28514442
ELL2_HUMAN	ELL2	physical	28514442
CDK9_HUMAN	CDK9	physical	28514442
EAF2_HUMAN	EAF2	physical	28514442
CCNT2_HUMAN	CCNT2	physical	28514442
ELL_HUMAN	ELL	physical	28514442
CCNT1_HUMAN	CCNT1	physical	28514442
HEXI1_HUMAN	HEXIM1	physical	28514442
PBX2_HUMAN	PBX2	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
	Note=A chromosomal aberration involving AFF4 is found in acute lymphoblastic leukemia (ALL). Insertion ins(5
	11)(q31
	q13q23) that forms a KMT2A/MLL1-AFF4 fusion protein.
616368
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AFF4_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-79, AND MASS SPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1058, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-32; SER-178;SER-210; SER-549; SER-617; TYR-668; SER-671; SER-694; SER-703;SER-706; SER-814; SER-836; SER-1043; SER-1055 AND SER-1058, AND MASSSPECTROMETRY.	19369195 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-487; SER-491; SER-703;SER-706; SER-1043; SER-1055; SER-1058 AND SER-1062, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-388; SER-389;SER-392; SER-694; SER-703; SER-706; TYR-712; SER-1043; SER-1055;SER-1058 AND SER-1062, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-124; SER-212;SER-222; SER-487; SER-514; THR-528; SER-549; SER-599; SER-694;SER-703; SER-706; SER-814; SER-836; SER-1043; SER-1055 AND SER-1058,AND MASS SPECTROMETRY.	18691976 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-499, AND MASSSPECTROMETRY.	17525332 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-387; SER-388; SER-389;SER-392; SER-549; SER-703 AND SER-706, AND MASS SPECTROMETRY.	17081983 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-674, AND MASSSPECTROMETRY.	18220336 [Abstract]