dbPTM

AFF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AFF1_HUMAN
UniProt AC	P51825
Protein Name	AF4/FMR2 family member 1
Gene Name	AFF1
Organism	Homo sapiens (Human).
Sequence Length	1210
Subcellular Localization	Nucleus .
Protein Description
Protein Sequence	MAAQSSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDASENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPERELSPLISLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSSNNSKGYCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEGPRSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRTSLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKALSGPEPAKDNVEDRTPEHFALVPLTESQGPPHSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSRPSSQSSKKEMLPPPPVSSSSQKPAKPALKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDRVGKAFKYLEAVLSFIECGIATESESQSSKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIASTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MAAQSSLYNDDR ---CCCCCHHCCCCH	19.99	28060719
6	Phosphorylation	--MAAQSSLYNDDRN --CCCCCHHCCCCHH	23.85	28060719
8	Phosphorylation	MAAQSSLYNDDRNLL CCCCCHHCCCCHHHH	20.63	28060719
9 (in isoform 2)	Phosphorylation	-	50.42	26471730
10 (in isoform 2)	Phosphorylation	-	42.56	21815630
13 (in isoform 2)	Phosphorylation	-	50.88	26471730
15 (in isoform 2)	Phosphorylation	-	4.96	26471730
45	Phosphorylation	IPLFGEPYKTAKGDE CCCCCCCCCCCCHHH	19.37	27422710
64	Phosphorylation	IQNMLGNYEEVKEFL HHHHHCCHHHHHHHH	16.21	28796482
71 (in isoform 2)	Phosphorylation	-	7.15	27642862
74	Ubiquitination	VKEFLSTKSHTHRLD HHHHHHCCCCHHCCC	36.54	-
83	Phosphorylation	HTHRLDASENRLGKP CHHCCCCCCCCCCCC	34.76	72272945
89	Acetylation	ASENRLGKPKYPLIP CCCCCCCCCCCCCCC	42.82	7964611
91	Acetylation	ENRLGKPKYPLIPDK CCCCCCCCCCCCCCC	64.09	7964623
98	Acetylation	KYPLIPDKGSSIPSS CCCCCCCCCCCCCCC	56.17	26051181
171	Phosphorylation	LGQEGFGSSHHKKGD CCCCCCCCCCCCCCC	25.00	28555341
172	Phosphorylation	GQEGFGSSHHKKGDR CCCCCCCCCCCCCCC	29.95	25627689
188	Phosphorylation	ADGDHCASVTDSAPE CCCCCCCCCCCCCCC	30.83	24144214
190	Phosphorylation	GDHCASVTDSAPERE CCCCCCCCCCCCCHH	23.03	24144214
192	Phosphorylation	HCASVTDSAPERELS CCCCCCCCCCCHHCC	36.04	24144214
199	Phosphorylation	SAPERELSPLISLPS CCCCHHCCCCCCCCC	16.95	22167270
203	Phosphorylation	RELSPLISLPSPVPP HHCCCCCCCCCCCCC	41.74	22167270
206	Phosphorylation	SPLISLPSPVPPLSP CCCCCCCCCCCCCCC	43.77	22167270
206 (in isoform 2)	Phosphorylation	-	43.77	27251275
212	Phosphorylation	PSPVPPLSPIHSNQQ CCCCCCCCCCCCCCC	27.81	29255136
213 (in isoform 2)	Phosphorylation	-	35.67	27251275
216	Phosphorylation	PPLSPIHSNQQTLPR CCCCCCCCCCCCCCC	36.73	29255136
219 (in isoform 2)	Phosphorylation	-	49.62	27251275
220	Phosphorylation	PIHSNQQTLPRTQGS CCCCCCCCCCCCCCC	29.03	29255136
237	Phosphorylation	VHGSSNNSKGYCPAK CCCCCCCCCCCCCCC	31.54	46159993
238	Acetylation	HGSSNNSKGYCPAKS CCCCCCCCCCCCCCC	56.90	18584413
240	Phosphorylation	SSNNSKGYCPAKSPK CCCCCCCCCCCCCHH	10.05	23403867
244	Acetylation	SKGYCPAKSPKDLAV CCCCCCCCCHHHEEE	53.05	18584425
245	Phosphorylation	KGYCPAKSPKDLAVK CCCCCCCCHHHEEEE	39.01	23401153
247	Acetylation	YCPAKSPKDLAVKVH CCCCCCHHHEEEEEC	72.90	18584437
252 (in isoform 2)	Phosphorylation	-	28.39	24719451
256	Acetylation	LAVKVHDKETPQDSL EEEEECCCCCCCCCC	48.92	26051181
281	Phosphorylation	FPPPSLPSKSVAMQQ CCCCCCCCCCCCCCC	42.74	24719451
283	Phosphorylation	PPSLPSKSVAMQQKP CCCCCCCCCCCCCCC	21.32	23186163
291	Phosphorylation	VAMQQKPTAYVRPMD CCCCCCCCEEEECCC	37.34	23186163
293	Phosphorylation	MQQKPTAYVRPMDGQ CCCCCCEEEECCCCC	10.24	23186163
305	Phosphorylation	DGQDQAPSESPELKP CCCCCCCCCCCCCCC	54.44	26657352
307	Phosphorylation	QDQAPSESPELKPLP CCCCCCCCCCCCCCC	28.19	26657352
311	Ubiquitination	PSESPELKPLPEDYR CCCCCCCCCCCHHHH	42.73	-
312 (in isoform 2)	Phosphorylation	-	66.81	27251275
324	Ubiquitination	YRQQTFEKTDLKVPA HHHHHHCCCCCCCCC	43.05	-
325	Phosphorylation	RQQTFEKTDLKVPAK HHHHHCCCCCCCCCC	39.09	20068231
328	Ubiquitination	TFEKTDLKVPAKAKL HHCCCCCCCCCCCCC	49.32	-
334	Ubiquitination	LKVPAKAKLTKLKMP CCCCCCCCCCCCCCC	56.85	-
339	Ubiquitination	KAKLTKLKMPSQSVE CCCCCCCCCCCCCHH	50.77	-
364	Phosphorylation	EEILKEMTHSWPPPL HHHHHHHHHCCCCCC	17.53	28450419
366	Phosphorylation	ILKEMTHSWPPPLTA HHHHHHHCCCCCCEE	31.92	30108239
372	Phosphorylation	HSWPPPLTAIHTPST HCCCCCCEEEECCCC	29.37	28450419
376	Phosphorylation	PPLTAIHTPSTAEPS CCCEEEECCCCCCCC	16.90	82900347
378	Phosphorylation	LTAIHTPSTAEPSKF CEEEECCCCCCCCCC	41.21	30108239
379	Phosphorylation	TAIHTPSTAEPSKFP EEEECCCCCCCCCCC	35.92	28450419
383	Phosphorylation	TPSTAEPSKFPFPTK CCCCCCCCCCCCCCC	38.13	30108239
383 (in isoform 2)	Phosphorylation	-	38.13	27251275
384	Acetylation	PSTAEPSKFPFPTKD CCCCCCCCCCCCCCC	68.70	26051181
389	Phosphorylation	PSKFPFPTKDSQHVS CCCCCCCCCCCCCHH	48.11	30108239
390	Ubiquitination	SKFPFPTKDSQHVSS CCCCCCCCCCCCHHH	56.47	-
403	Acetylation	SSVTQNQKQYDTSSK HHHCCCCHHCCCCCC	59.37	25953088
403	Ubiquitination	SSVTQNQKQYDTSSK HHHCCCCHHCCCCCC	59.37	-
534	Phosphorylation	APPEGPRSTEPPRRH CCCCCCCCCCCCCCC	40.01	28985074
544	Phosphorylation	PPRRHPESKGSSDSA CCCCCCCCCCCCCCC	46.93	72840311
560	Acetylation	SQEHSESKDPPPKSS HHHHHHCCCCCCCCC	70.92	26051181
582	Acetylation	PEAPHPGKRSCQKSP CCCCCCCCCCCCCCC	45.66	25953088
584	Phosphorylation	APHPGKRSCQKSPAQ CCCCCCCCCCCCCCC	25.12	23403867
588	Phosphorylation	GKRSCQKSPAQQEPP CCCCCCCCCCCCCCC	10.29	23401153
595 (in isoform 2)	Phosphorylation	-	41.03	27251275
603	Acetylation	QRQTVGTKQPKKPVK CCCCCCCCCCCCCCC	59.69	25953088
617	Phosphorylation	KASARAGSRTSLQGE CCHHCCCCCCCCCCC	31.25	28555341
619	Phosphorylation	SARAGSRTSLQGERE HHCCCCCCCCCCCCC	35.17	18691976
620	Phosphorylation	ARAGSRTSLQGEREP HCCCCCCCCCCCCCC	20.00	63731909
627 (in isoform 2)	Phosphorylation	-	44.98	27251275
660	Acetylation	AAASNEPKPAVPPSS CHHCCCCCCCCCCCH	39.16	26051181
666	Phosphorylation	PKPAVPPSSEKKKHK CCCCCCCCHHHHHCC	45.09	25159151
667	Phosphorylation	KPAVPPSSEKKKHKS CCCCCCCHHHHHCCC	60.24	25159151
680	Phosphorylation	KSSLPAPSKALSGPE CCCCCCCCHHHCCCC	32.44	113310181
681	Acetylation	SSLPAPSKALSGPEP CCCCCCCHHHCCCCC	52.89	19608861
684	Phosphorylation	PAPSKALSGPEPAKD CCCCHHHCCCCCCCC	58.10	23401153
687 (in isoform 2)	Phosphorylation	-	62.51	27251275
688	Acetylation	KALSGPEPAKDNVED HHHCCCCCCCCCCCC	47.98	19608861
697	Phosphorylation	KDNVEDRTPEHFALV CCCCCCCCCCCEEEE	45.40	29255136
704 (in isoform 2)	Phosphorylation	-	3.07	27251275
707	Phosphorylation	HFALVPLTESQGPPH CEEEEECCCCCCCCC	27.76	28464451
709	Phosphorylation	ALVPLTESQGPPHSG EEEECCCCCCCCCCC	34.89	30576142
715	Phosphorylation	ESQGPPHSGSGSRTS CCCCCCCCCCCCCCC	40.19	18691976
717	Phosphorylation	QGPPHSGSGSRTSGC CCCCCCCCCCCCCCC	36.29	25159151
719	Phosphorylation	PPHSGSGSRTSGCRQ CCCCCCCCCCCCCCE	33.80	27080861
734	Phosphorylation	AVVVQEDSRKDRLPL EEEEECCCCCCCCCC	40.24	30229599
750	Phosphorylation	LRDTKLLSPLRDTPP CCCCCCCCCCCCCCC	32.12	25159151
755	Phosphorylation	LLSPLRDTPPPQSLM CCCCCCCCCCCCHHH	31.12	25159151
757 (in isoform 2)	Phosphorylation	-	41.47	24719451
760	Phosphorylation	RDTPPPQSLMVKITL CCCCCCCHHHHHHHH	24.98	20068231
766	Phosphorylation	QSLMVKITLDLLSRI CHHHHHHHHHHHHCC	14.43	15302935
789	Acetylation	RQRKAEDKQPPAGKK HHCCCCCCCCCCCCC	57.09	26051181
803	Phosphorylation	KHSSEKRSSDSSSKL CCCCCCCCCCCHHHH	49.95	46159997
809	Acetylation	RSSDSSSKLAKKRKG CCCCCHHHHHHHHHC	55.18	25953088
826	Ubiquitination	ERDCDNKKIRLEKEI HHHCCCCCHHHHHHH	39.03	-
831	Acetylation	NKKIRLEKEIKSQSS CCCHHHHHHHHHCCC	70.14	26051181
835	Phosphorylation	RLEKEIKSQSSSSSS HHHHHHHHCCCCCCC	40.74	10508641
837	Phosphorylation	EKEIKSQSSSSSSSH HHHHHHCCCCCCCCC	39.00	158919
838	Phosphorylation	KEIKSQSSSSSSSHK HHHHHCCCCCCCCCC	26.97	10509639
839	Phosphorylation	EIKSQSSSSSSSHKE HHHHCCCCCCCCCCC	39.21	27251275
840	Phosphorylation	IKSQSSSSSSSHKES HHHCCCCCCCCCCCC	35.87	27251275
841	Phosphorylation	KSQSSSSSSSHKESS HHCCCCCCCCCCCCC	37.50	30576142
842	Phosphorylation	SQSSSSSSSHKESSK HCCCCCCCCCCCCCC	38.11	24719451
843	Phosphorylation	QSSSSSSSHKESSKT CCCCCCCCCCCCCCC	40.29	27251275
847	Phosphorylation	SSSSHKESSKTKPSR CCCCCCCCCCCCCCC	41.77	8656097
847 (in isoform 2)	Phosphorylation	-	41.77	27251275
848	Phosphorylation	SSSHKESSKTKPSRP CCCCCCCCCCCCCCC	45.49	30576142
849 (in isoform 2)	Phosphorylation	-	70.60	24719451
871	Phosphorylation	MLPPPPVSSSSQKPA CCCCCCCCCCCCCCC	30.21	26074081
872	Phosphorylation	LPPPPVSSSSQKPAK CCCCCCCCCCCCCCC	33.95	26074081
873	Phosphorylation	PPPPVSSSSQKPAKP CCCCCCCCCCCCCCH	29.31	25627689
874	Phosphorylation	PPPVSSSSQKPAKPA CCCCCCCCCCCCCHH	44.14	25627689
932	Phosphorylation	SSSEHKGSSGDTANP CCCCCCCCCCCCCCC	35.63	68698031
950	Phosphorylation	PSLPNGNSKPGKPQV CCCCCCCCCCCCCCC	41.44	46160003
1039	Phosphorylation	LKSFSDATAPTQEKI CCCCCCCCCCCHHHH	37.85	46160009
1042	Phosphorylation	FSDATAPTQEKIFAV CCCCCCCCHHHHHHH	47.07	46160015
1067	Malonylation	MAMFRCKKDIAIKYS HHHHHCCHHHHHHHH	58.97	26320211
1072	Malonylation	CKKDIAIKYSRTLNK CCHHHHHHHHHHHHH	28.31	26320211
1092 (in isoform 3)	Phosphorylation	-	35.11	30266825
1099 (in isoform 2)	Phosphorylation	-	32.88	30266825
1166	Phosphorylation	WEQAEALTRKNKEFF HHHHHHHHHHCHHHH	46.79	20071362
1209	Phosphorylation	QLQELTKTP------ HHHHHHCCC------	29.06	22617229
1217 (in isoform 2)	Phosphorylation	-		24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SIAH1	Q8IUQ4	PMID:15221006

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AFF1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AFF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CCNT1_HUMAN	CCNT1	physical	19956800
5NTD_HUMAN	NT5E	physical	19956800
ENL_HUMAN	MLLT1	physical	20153263
CCNT1_HUMAN	CCNT1	physical	20153263
CDK9_HUMAN	CDK9	physical	20153263
AFF4_HUMAN	AFF4	physical	20153263
AFF1_HUMAN	AFF1	physical	20153263
KMT2A_HUMAN	KMT2A	physical	21030982
AFF1_HUMAN	AFF1	physical	21030982
CCNT1_HUMAN	CCNT1	physical	21030982
CDK9_HUMAN	CDK9	physical	21030982
BRD4_HUMAN	BRD4	physical	21030982
TF65_HUMAN	RELA	physical	21030982
NPM_HUMAN	NPM1	physical	21030982
ENL_HUMAN	MLLT1	physical	21030982
NEC1_HUMAN	PCSK1	physical	21030982
AF9_HUMAN	MLLT3	physical	21030982
AF10_HUMAN	MLLT10	physical	21030982
DOT1L_HUMAN	DOT1L	physical	21030982
RPB1_HUMAN	POLR2A	physical	21030982
DDX6_HUMAN	DDX6	physical	21030982
HEXI1_HUMAN	HEXIM1	physical	21030982
H31T_HUMAN	HIST3H3	physical	21030982
SIAH1_HUMAN	SIAH1	physical	15221006
SIAH2_HUMAN	SIAH2	physical	15221006
AFF1_HUMAN	AFF1	physical	15221006
ENL_HUMAN	MLLT1	physical	15856011
ELL2_HUMAN	ELL2	physical	24367103
ENL_HUMAN	MLLT1	physical	24367103
LARP7_HUMAN	LARP7	physical	24367103
HEXI1_HUMAN	HEXIM1	physical	24367103
CDK9_HUMAN	CDK9	physical	24367103
CCNT1_HUMAN	CCNT1	physical	24367103

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AFF1_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-681, AND MASS SPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206 AND SER-750, ANDMASS SPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206; SER-212; SER-216;SER-245; SER-588; THR-697; SER-750 AND THR-1209, AND MASSSPECTROMETRY.	19369195 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206; SER-212; THR-220;SER-750 AND THR-755, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-199; SER-206; SER-212;SER-588; SER-750; THR-755 AND THR-1209, AND MASS SPECTROMETRY.	18691976 [Abstract]
"Large-scale characterization of HeLa cell nuclear phosphoproteins."; Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-588 AND THR-766, ANDMASS SPECTROMETRY.	15302935 [Abstract]