dbPTM

AF9_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AF9_HUMAN
UniProt AC	P42568
Protein Name	Protein AF-9
Gene Name	MLLT3 {ECO:0000312\|HGNC:HGNC:7136}
Organism	Homo sapiens (Human).
Sequence Length	568
Subcellular Localization	Nucleus . Chromosome . Colocalizes with acylated histone H3 (PubMed:25417107, PubMed:27105114). Colocalizes with histone H3 crotonylated at 'Lys-18' (H3K18cr) (PubMed:27105114).
Protein Description	Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. [PubMed: 20159561]
Protein Sequence	MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGGDPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKDSREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKKAPSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKKKSTLPPFDDIVDPNDSDVEENISSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEESDEVEDNDNDSEMERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSKSDKQIKNGECDKAYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTVRKLQSYLETSGTS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
10	Acetylation	SSCAVQVKLELGHRA CCCEEEEEEECCCCC	21.10	25953088
58	Phosphorylation	VVFHLHESFPRPKRV HHHHHHHHCCCCCCC	29.78	-
71	Phosphorylation	RVCKDPPYKVEESGY CCCCCCCCCCCCCCC	32.81	26074081
76	Phosphorylation	PPYKVEESGYAGFIL CCCCCCCCCCCEEEE	24.49	26074081
78	Phosphorylation	YKVEESGYAGFILPI CCCCCCCCCEEEEEE	16.90	26074081
88	Phosphorylation	FILPIEVYFKNKEEP EEEEEEEEECCCCCC	8.95	26074081
123	Phosphorylation	HLRCEKLTFNNPTED CEEEEECCCCCCCHH	35.07	22817900
151	Phosphorylation	RSIHTSSSSSSSSSS CCCCCCCCCCCCCCC	33.72	-
152	Phosphorylation	SIHTSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
153	Phosphorylation	IHTSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
154	Phosphorylation	HTSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
155	Phosphorylation	TSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
156	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
157	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
158	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
159	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
160	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
161	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
162	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
163	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
164	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
165	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
166	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
167	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
168	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
169	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
170	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
171	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
172	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
173	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
174	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
175	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
176	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
177	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
178	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
179	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
180	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	19664994
181	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
182	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
183	Phosphorylation	SSSSSSSSSSSSSSS CCCCCCCCCCCCCCC	35.87	-
184	Phosphorylation	SSSSSSSSSSSSSST CCCCCCCCCCCCCCC	35.87	-
192	Phosphorylation	SSSSSSTSFSKPHKL CCCCCCCCCCCHHHH	29.48	24719451
211	O-linked_Glycosylation	KEKPSKDSREHKSAF HCCCCCCHHHHHHHH	43.22	30379171
219	Acetylation	REHKSAFKEPSRDHN HHHHHHHCCCCCCCC	69.32	25953088
235	Acetylation	SSKESSKKPKENKPL CCCHHCCCCCCCCCC	64.37	19822071
237	Acetylation	KESSKKPKENKPLKE CHHCCCCCCCCCCCH	80.80	-
240	Acetylation	SKKPKENKPLKEEKI CCCCCCCCCCCHHCC	53.47	23749302
251	Acetylation	EEKIVPKMAFKEPKP HHCCCCCCCCCCCCC	4.31	-
254	Acetylation	IVPKMAFKEPKPMSK CCCCCCCCCCCCCCC	64.67	23749302
257	Acetylation	KMAFKEPKPMSKEPK CCCCCCCCCCCCCCC	55.21	26051181
264	Sumoylation	KPMSKEPKPDSNLLT CCCCCCCCCCCCCEE	63.65	-
264	Sumoylation	KPMSKEPKPDSNLLT CCCCCCCCCCCCCEE	63.65	-
278	Acetylation	TITSGQDKKAPSKRP EEECCCCCCCCCCCC	42.84	25953088
283	Acetylation	QDKKAPSKRPPISDS CCCCCCCCCCCCCCH	68.79	25953088
288	Phosphorylation	PSKRPPISDSEELSA CCCCCCCCCHHHHHH	40.64	25159151
290	Phosphorylation	KRPPISDSEELSAKK CCCCCCCHHHHHHHH	26.61	29978859
294	Phosphorylation	ISDSEELSAKKRKKS CCCHHHHHHHHCCCC	41.12	25159151
296	Acetylation	DSEELSAKKRKKSSS CHHHHHHHHCCCCCH	50.13	25953088
300	Methylation	LSAKKRKKSSSEALF HHHHHCCCCCHHHHH	60.65	115973163
301	Phosphorylation	SAKKRKKSSSEALFK HHHHCCCCCHHHHHH	41.97	29116813
302	Phosphorylation	AKKRKKSSSEALFKS HHHCCCCCHHHHHHH	40.87	21815630
303	Phosphorylation	KKRKKSSSEALFKSF HHCCCCCHHHHHHHH	33.70	28464451
308	Methylation	SSSEALFKSFSSAPP CCHHHHHHHHHCCCC	51.98	115973171
309	Phosphorylation	SSEALFKSFSSAPPL CHHHHHHHHHCCCCE	24.03	25159151
311	Phosphorylation	EALFKSFSSAPPLIL HHHHHHHHCCCCEEE	32.45	25627689
312	Phosphorylation	ALFKSFSSAPPLILT HHHHHHHCCCCEEEE	42.66	27251275
319	Phosphorylation	SAPPLILTCSADKKQ CCCCEEEEEECCHHH	9.45	25159151
321	Phosphorylation	PPLILTCSADKKQIK CCEEEEEECCHHHHC	33.98	21815630
324	Acetylation	ILTCSADKKQIKDKS EEEEECCHHHHCCHH	46.42	25953088
330	Acetylation	DKKQIKDKSHVKMGK CHHHHCCHHHCCCCE	37.14	11794765
331	Phosphorylation	KKQIKDKSHVKMGKV HHHHCCHHHCCCCEE	43.58	-
334	Acetylation	IKDKSHVKMGKVKIE HCCHHHCCCCEEEEE	36.19	11794775
337	Acetylation	KSHVKMGKVKIESET HHHCCCCEEEEEECC	36.54	11794785
339	Sumoylation	HVKMGKVKIESETSE HCCCCEEEEEECCCC	44.22	-
339	Acetylation	HVKMGKVKIESETSE HCCCCEEEEEECCCC	44.22	26051181
339	Sumoylation	HVKMGKVKIESETSE HCCCCEEEEEECCCC	44.22	28112733
350	Phosphorylation	ETSEKKKSTLPPFDD CCCCCCCCCCCCCCC	44.02	30576142
364	Phosphorylation	DIVDPNDSDVEENIS CCCCCCCCHHHHHHC	50.63	20873877
371	Phosphorylation	SDVEENISSKSDSEQ CHHHHHHCCCCCCCC	43.21	30576142
372	Phosphorylation	DVEENISSKSDSEQP HHHHHHCCCCCCCCC	32.17	27732954
385	Phosphorylation	QPSPASSSSSSSSSF CCCCCCCCCCCCCCC	31.57	-
386	Phosphorylation	PSPASSSSSSSSSFT CCCCCCCCCCCCCCC	35.87	-
387	Phosphorylation	SPASSSSSSSSSFTP CCCCCCCCCCCCCCH	35.87	28787133
388	Phosphorylation	PASSSSSSSSSFTPS CCCCCCCCCCCCCHH	35.87	-
389	Phosphorylation	ASSSSSSSSSFTPSQ CCCCCCCCCCCCHHH	31.76	30576142
412	Phosphorylation	SIMKDLHSDDNEEES HHHHHHHCCCCHHHC	54.69	21712546
419	Phosphorylation	SDDNEEESDEVEDND CCCCHHHCCCCCCCC	42.21	21712546
429	Phosphorylation	VEDNDNDSEMERPVN CCCCCCHHHCCCCCC	45.13	21712546
446	Phosphorylation	GSRSRRVSLSDGSDS CCCCCEEECCCCCCC	21.97	30177828
448	Phosphorylation	RSRRVSLSDGSDSES CCCEEECCCCCCCCC	32.21	30177828
451	Phosphorylation	RVSLSDGSDSESSSA EEECCCCCCCCCCCC	42.84	30177828
453	Phosphorylation	SLSDGSDSESSSASS ECCCCCCCCCCCCCC	40.87	30177828
455	Phosphorylation	SDGSDSESSSASSPL CCCCCCCCCCCCCCC	33.28	30177828
456	Phosphorylation	DGSDSESSSASSPLH CCCCCCCCCCCCCCC	26.35	30177828
457	Phosphorylation	GSDSESSSASSPLHH CCCCCCCCCCCCCCC	41.26	30177828
459	Phosphorylation	DSESSSASSPLHHEP CCCCCCCCCCCCCCC	34.14	30177828
460	Phosphorylation	SESSSASSPLHHEPP CCCCCCCCCCCCCCC	31.06	30177828
473	Phosphorylation	PPPPLLKTNNNQILE CCCCCCCCCCCCEEE	43.05	24732914
483	Phosphorylation	NQILEVKSPIKQSKS CCEEEECCCCCCCCC	36.71	25159151
488	Phosphorylation	VKSPIKQSKSDKQIK ECCCCCCCCCHHHCC	29.17	22617229
557	Ubiquitination	LDKTTVRKLQSYLET CCHHHHHHHHHHHHH	46.68	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of AF9_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AF9_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AF9_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
APBP2_HUMAN	APPBP2	physical	16189514
BCOR_HUMAN	BCOR	physical	12776190
RBM48_HUMAN	RBM48	physical	16169070
EF1A1_HUMAN	EEF1A1	physical	16169070
PTN_HUMAN	PTN	physical	16169070
LRIF1_HUMAN	LRIF1	physical	16169070
DOT1L_HUMAN	DOT1L	physical	16636056
DOT1L_HUMAN	DOT1L	physical	17332896
AFF4_HUMAN	AFF4	physical	20431927
AFF1_HUMAN	AFF1	physical	20431927
BCOR_HUMAN	BCOR	physical	20431927
CDK9_HUMAN	CDK9	physical	20431927
DOT1L_HUMAN	DOT1L	physical	20431927
AFF3_HUMAN	AFF3	physical	20431927
ACACA_HUMAN	ACACA	physical	20431927
SIR1_HUMAN	SIRT1	physical	20431927
CCNT1_HUMAN	CCNT1	physical	20431927
NIPBL_HUMAN	NIPBL	physical	20431927
EAF1_HUMAN	EAF1	physical	20431927
ELL3_HUMAN	ELL3	physical	20431927
PCGF1_HUMAN	PCGF1	physical	20431927
ENL_HUMAN	MLLT1	physical	20431927
EF2_HUMAN	EEF2	physical	20431927
ELL_HUMAN	ELL	physical	20431927
BCOR_HUMAN	BCOR	physical	20854876
DOT1L_HUMAN	DOT1L	physical	20854876
AFF1_HUMAN	AFF1	physical	20854876
AFF3_HUMAN	AFF3	physical	20854876
AFF4_HUMAN	AFF4	physical	20854876
AF10_HUMAN	MLLT10	physical	20854876
CCNT1_HUMAN	CCNT1	physical	20854876
CCNT2_HUMAN	CCNT2	physical	20854876
ENL_HUMAN	MLLT1	physical	20854876
ELL_HUMAN	ELL	physical	20854876
CDK9_HUMAN	CDK9	physical	20854876
RING2_HUMAN	RNF2	physical	20854876
DOT1L_HUMAN	DOT1L	physical	21896721
KMT2A_HUMAN	KMT2A	physical	21896721
AFF1_HUMAN	AFF1	physical	21896721
AFF4_HUMAN	AFF4	physical	21873227
CDK9_HUMAN	CDK9	physical	21873227
ELL2_HUMAN	ELL2	physical	21873227
PAF1_HUMAN	PAF1	physical	21873227
A4_HUMAN	APP	physical	21832049
ELL_HUMAN	ELL	physical	21729782
EAF1_HUMAN	EAF1	physical	21729782
CDK9_HUMAN	CDK9	physical	21729782
AFF4_HUMAN	AFF4	physical	21729782
ELL2_HUMAN	ELL2	physical	21729782
AFF1_HUMAN	AFF1	physical	21729782
ENL_HUMAN	MLLT1	physical	21729782
CCNT1_HUMAN	CCNT1	physical	21729782
CCNT2_HUMAN	CCNT2	physical	21729782
ENPL_HUMAN	HSP90B1	physical	20159978
H33_HUMAN	H3F3A	physical	25417107
DOT1L_HUMAN	DOT1L	physical	25417107
CDK9_HUMAN	CDK9	physical	25417107
ELL2_HUMAN	ELL2	physical	25417107
AFF4_HUMAN	AFF4	physical	25417107

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AF9_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-412; SER-419 ANDSER-429, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288, AND MASSSPECTROMETRY.	19369195 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-288; SER-294; SER-412;SER-419; SER-429 AND SER-483, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294; SER-412; SER-419;SER-429 AND SER-483, AND MASS SPECTROMETRY.	18691976 [Abstract]