dbPTM

AF10_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	AF10_HUMAN
UniProt AC	P55197
Protein Name	Protein AF-10 {ECO:0000305}
Gene Name	MLLT10 {ECO:0000312\|HGNC:HGNC:16063}
Organism	Homo sapiens (Human).
Sequence Length	1068
Subcellular Localization	Nucleus .
Protein Description	Probably involved in transcriptional regulation. In vitro or as fusion protein with KMT2A/MLL1 has transactivation activity. Binds to cruciform DNA. In cells, binding to unmodified histone H3 regulates DOT1L functions including histone H3 'Lys-79' dimethylation (H3K79me2) and gene activation. [PubMed: 26439302]
Protein Sequence	MVSSDRPVSLEDEVSHSMKEMIGGCCVCSDERGWAENPLVYCDGHGCSVAVHQACYGIVQVPTGPWFCRKCESQERAARVRCELCPHKDGALKRTDNGGWAHVVCALYIPEVQFANVSTMEPIVLQSVPHDRYNKTCYICDEQGRESKAATGACMTCNKHGCRQAFHVTCAQFAGLLCEEEGNGADNVQYCGYCKYHFSKLKKSKRGSNRSYDQSLSDSSSHSQDKHHEKEKKKYKEKDKHKQKHKKQPEPSPALVPSLTVTTEKTYTSTSNNSISGSLKRLEDTTARFTNANFQEVSAHTSSGKDVSETRGSEGKGKKSSAHSSGQRGRKPGGGRNPGTTVSAASPFPQGSFSGTPGSVKSSSGSSVQSPQDFLSFTDSDLRNDSYSHSQQSSATKDVHKGESGSQEGGVNSFSTLIGLPSTSAVTSQPKSFENSPGDLGNSSLPTAGYKRAQTSGIEEETVKEKKRKGNKQSKHGPGRPKGNKNQENVSHLSVSSASPTSSVASAAGSITSSSLQKSPTLLRNGSLQSLSVGSSPVGSEISMQYRHDGACPTTTFSELLNAIHNGIYNSNDVAVSFPNVVSGSGSSTPVSSSHLPQQSSGHLQQVGALSPSAVSSAAPAVATTQANTLSGSSLSQAPSHMYGNRSNSSMAALIAQSENNQTDQDLGDNSRNLVGRGSSPRGSLSPRSPVSSLQIRYDQPGNSSLENLPPVAASIEQLLERQWSEGQQFLLEQGTPSDILGMLKSLHQLQVENRRLEEQIKNLTAKKERLQLLNAQLSVPFPTITANPSPSHQIHTFSAQTAPTTDSLNSSKSPHIGNSFLPDNSLPVLNQDLTSSGQSTSSSSALSTPPPAGQSPAQQGSGVSGVQQVNGVTVGALASGMQPVTSTIPAVSAVGGIIGALPGNQLAINGIVGALNGVMQTPVTMSQNPTPLTHTTVPPNATHPMPATLTNSASGLGLLSDQQRQILIHQQQFQQLLNSQQLTPEQHQAFLYQLMQHHHQQHHQPELQQLQIPGPTQIPINNLLAGTQAPPLHTATTNPFLTIHGDNASQKVARLSDKTGPVAQEKS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MVSSDRPVSL -----CCCCCCCCCH	38.08	24043423
4	Phosphorylation	----MVSSDRPVSLE ----CCCCCCCCCHH	42.71	24043423
9	Phosphorylation	VSSDRPVSLEDEVSH CCCCCCCCHHHHHHH	28.82	24043423
15	Phosphorylation	VSLEDEVSHSMKEMI CCHHHHHHHHHHHHH	14.06	24043423
17	Phosphorylation	LEDEVSHSMKEMIGG HHHHHHHHHHHHHCC	24.84	24043423
41	Phosphorylation	WAENPLVYCDGHGCS CCCCCEEEECCCCCC	7.78	30257219
48	Phosphorylation	YCDGHGCSVAVHQAC EECCCCCCHHHHHHH	20.08	30257219
56	Phosphorylation	VAVHQACYGIVQVPT HHHHHHHCEEEECCC	16.95	30257219
147	Phosphorylation	CDEQGRESKAATGAC ECCCCCCCHHCCCCC	26.66	-
211	Phosphorylation	SKRGSNRSYDQSLSD CCCCCCCCCCCCCCC	36.99	23090842
212	Phosphorylation	KRGSNRSYDQSLSDS CCCCCCCCCCCCCCC	18.11	23090842
215	Phosphorylation	SNRSYDQSLSDSSSH CCCCCCCCCCCCCCC	27.26	28450419
217	Phosphorylation	RSYDQSLSDSSSHSQ CCCCCCCCCCCCCCC	40.47	25849741
219	Phosphorylation	YDQSLSDSSSHSQDK CCCCCCCCCCCCCHH	30.30	28450419
220	Phosphorylation	DQSLSDSSSHSQDKH CCCCCCCCCCCCHHH	36.74	30576142
221	Phosphorylation	QSLSDSSSHSQDKHH CCCCCCCCCCCHHHH	30.98	28450419
223	Phosphorylation	LSDSSSHSQDKHHEK CCCCCCCCCHHHHHH	41.86	28450419
235	Phosphorylation	HEKEKKKYKEKDKHK HHHHHHHHHHHHHHH	33.01	26657352
236	Acetylation	EKEKKKYKEKDKHKQ HHHHHHHHHHHHHHH	68.14	133107
240	Acetylation	KKYKEKDKHKQKHKK HHHHHHHHHHHHHCC	64.86	133119
242	Acetylation	YKEKDKHKQKHKKQP HHHHHHHHHHHCCCC	67.78	133115
246	Acetylation	DKHKQKHKKQPEPSP HHHHHHHCCCCCCCC	61.20	133111
252	Phosphorylation	HKKQPEPSPALVPSL HCCCCCCCCCCCCCE	22.57	25159151
258	Phosphorylation	PSPALVPSLTVTTEK CCCCCCCCEEEEECE	29.23	28555341
262	Phosphorylation	LVPSLTVTTEKTYTS CCCCEEEEECEEEEE	24.81	26270265
263	Phosphorylation	VPSLTVTTEKTYTST CCCEEEEECEEEEEC	31.02	26270265
266	Phosphorylation	LTVTTEKTYTSTSNN EEEEECEEEEECCCC	26.29	26270265
267	Phosphorylation	TVTTEKTYTSTSNNS EEEECEEEEECCCCC	15.12	26270265
268	Phosphorylation	VTTEKTYTSTSNNSI EEECEEEEECCCCCC	30.66	26270265
269	O-linked_Glycosylation	TTEKTYTSTSNNSIS EECEEEEECCCCCCC	21.30	31492838
269	Phosphorylation	TTEKTYTSTSNNSIS EECEEEEECCCCCCC	21.30	26270265
269	O-linked_Glycosylation	TTEKTYTSTSNNSIS EECEEEEECCCCCCC	21.30	20068230
270	Phosphorylation	TEKTYTSTSNNSISG ECEEEEECCCCCCCE	27.89	26270265
271	Phosphorylation	EKTYTSTSNNSISGS CEEEEECCCCCCCEE	33.51	26270265
274	Phosphorylation	YTSTSNNSISGSLKR EEECCCCCCCEEHHH	23.52	26270265
276	Phosphorylation	STSNNSISGSLKRLE ECCCCCCCEEHHHHH	23.34	26270265
278	Phosphorylation	SNNSISGSLKRLEDT CCCCCCEEHHHHHHH	24.75	30576142
280	Methylation	NSISGSLKRLEDTTA CCCCEEHHHHHHHCH	57.54	110868411
280	Sumoylation	NSISGSLKRLEDTTA CCCCEEHHHHHHHCH	57.54	-
280	Acetylation	NSISGSLKRLEDTTA CCCCEEHHHHHHHCH	57.54	25953088
280	Sumoylation	NSISGSLKRLEDTTA CCCCEEHHHHHHHCH	57.54	25218447
298	Phosphorylation	NANFQEVSAHTSSGK CCCHHHEECCCCCCC	17.36	28555341
301	Phosphorylation	FQEVSAHTSSGKDVS HHHEECCCCCCCCHH	25.06	25627689
302	Phosphorylation	QEVSAHTSSGKDVSE HHEECCCCCCCCHHH	27.50	25627689
303	Phosphorylation	EVSAHTSSGKDVSET HEECCCCCCCCHHHC	51.52	28555341
305	Acetylation	SAHTSSGKDVSETRG ECCCCCCCCHHHCCC	57.83	26051181
308	Phosphorylation	TSSGKDVSETRGSEG CCCCCCHHHCCCCCC	43.30	28348404
310	Phosphorylation	SGKDVSETRGSEGKG CCCCHHHCCCCCCCC	32.59	30576142
313	Phosphorylation	DVSETRGSEGKGKKS CHHHCCCCCCCCCCC	39.63	30576142
340	Phosphorylation	GGGRNPGTTVSAASP CCCCCCCCEECCCCC	25.86	27732954
341	Phosphorylation	GGRNPGTTVSAASPF CCCCCCCEECCCCCC	20.55	27732954
343	O-linked_Glycosylation	RNPGTTVSAASPFPQ CCCCCEECCCCCCCC	18.94	31492838
343	Phosphorylation	RNPGTTVSAASPFPQ CCCCCEECCCCCCCC	18.94	27732954
346	Phosphorylation	GTTVSAASPFPQGSF CCEECCCCCCCCCCC	27.13	27732954
352	Phosphorylation	ASPFPQGSFSGTPGS CCCCCCCCCCCCCCC	15.81	27732954
354	Phosphorylation	PFPQGSFSGTPGSVK CCCCCCCCCCCCCEE	43.38	27732954
362	Phosphorylation	GTPGSVKSSSGSSVQ CCCCCEECCCCCCCC	27.81	30108239
363	Phosphorylation	TPGSVKSSSGSSVQS CCCCEECCCCCCCCC	32.76	30108239
364	Phosphorylation	PGSVKSSSGSSVQSP CCCEECCCCCCCCCH	50.19	30108239
366	Phosphorylation	SVKSSSGSSVQSPQD CEECCCCCCCCCHHH	29.32	30108239
367	Phosphorylation	VKSSSGSSVQSPQDF EECCCCCCCCCHHHH	27.92	30108239
370	Phosphorylation	SSGSSVQSPQDFLSF CCCCCCCCHHHHHHC	23.52	30108239
376	Phosphorylation	QSPQDFLSFTDSDLR CCHHHHHHCCCHHHC	26.54	28111955
378	Phosphorylation	PQDFLSFTDSDLRND HHHHHHCCCHHHCCC	31.08	28111955
380	Phosphorylation	DFLSFTDSDLRNDSY HHHHCCCHHHCCCCC	35.17	28111955
388	Phosphorylation	DLRNDSYSHSQQSSA HHCCCCCCCCCCCCC	22.18	26425664
394	Phosphorylation	YSHSQQSSATKDVHK CCCCCCCCCCCCCCC	35.30	28985074
404	Phosphorylation	KDVHKGESGSQEGGV CCCCCCCCCCCCCCC	53.03	20068231
406	Phosphorylation	VHKGESGSQEGGVNS CCCCCCCCCCCCCCC	34.05	17525332
413	Phosphorylation	SQEGGVNSFSTLIGL CCCCCCCCHHHHCCC	20.40	27732954
415	Phosphorylation	EGGVNSFSTLIGLPS CCCCCCHHHHCCCCC	23.33	27732954
416	Phosphorylation	GGVNSFSTLIGLPST CCCCCHHHHCCCCCC	22.00	27732954
422	Phosphorylation	STLIGLPSTSAVTSQ HHHCCCCCCCCCCCC	39.78	23312004
423	Phosphorylation	TLIGLPSTSAVTSQP HHCCCCCCCCCCCCC	20.83	23312004
424	Phosphorylation	LIGLPSTSAVTSQPK HCCCCCCCCCCCCCC	25.69	23312004
432	Phosphorylation	AVTSQPKSFENSPGD CCCCCCCCCCCCCCC	44.47	25159151
436	Phosphorylation	QPKSFENSPGDLGNS CCCCCCCCCCCCCCC	24.06	23401153
443	Phosphorylation	SPGDLGNSSLPTAGY CCCCCCCCCCCCCCH	31.30	28450419
444	Phosphorylation	PGDLGNSSLPTAGYK CCCCCCCCCCCCCHH	42.22	28450419
447	Phosphorylation	LGNSSLPTAGYKRAQ CCCCCCCCCCHHHHH	37.70	28985074
451	Acetylation	SLPTAGYKRAQTSGI CCCCCCHHHHHCCCC	40.10	25953088
455	Phosphorylation	AGYKRAQTSGIEEET CCHHHHHCCCCCHHH	28.12	27134283
456	Phosphorylation	GYKRAQTSGIEEETV CHHHHHCCCCCHHHH	26.20	21815630
491	Phosphorylation	NKNQENVSHLSVSSA CCCCCCCCEEEECCC	30.23	23663014
494	Phosphorylation	QENVSHLSVSSASPT CCCCCEEEECCCCCC	17.96	23663014
496	Phosphorylation	NVSHLSVSSASPTSS CCCEEEECCCCCCCH	19.46	23663014
497	Phosphorylation	VSHLSVSSASPTSSV CCEEEECCCCCCCHH	30.52	23663014
499	Phosphorylation	HLSVSSASPTSSVAS EEEECCCCCCCHHHH	30.56	23663014
501	Phosphorylation	SVSSASPTSSVASAA EECCCCCCCHHHHHH	31.01	23663014
502	Phosphorylation	VSSASPTSSVASAAG ECCCCCCCHHHHHHC	26.63	23663014
503	Phosphorylation	SSASPTSSVASAAGS CCCCCCCHHHHHHCC	24.95	23663014
506	Phosphorylation	SPTSSVASAAGSITS CCCCHHHHHHCCCCC	19.25	23663014
510	Phosphorylation	SVASAAGSITSSSLQ HHHHHHCCCCCHHHC	20.26	27080861
512	Phosphorylation	ASAAGSITSSSLQKS HHHHCCCCCHHHCCC	25.01	25921289
519	Phosphorylation	TSSSLQKSPTLLRNG CCHHHCCCCCEECCC	15.34	23403867
521	Phosphorylation	SSLQKSPTLLRNGSL HHHCCCCCEECCCCC	45.58	23403867
527	Phosphorylation	PTLLRNGSLQSLSVG CCEECCCCCCEEEEC	27.13	28450419
530	Phosphorylation	LRNGSLQSLSVGSSP ECCCCCCEEEECCCC	28.09	28450419
532	Phosphorylation	NGSLQSLSVGSSPVG CCCCCEEEECCCCCC	29.67	28450419
535	Phosphorylation	LQSLSVGSSPVGSEI CCEEEECCCCCCCEE	29.34	22617229
647	Phosphorylation	SHMYGNRSNSSMAAL CHHCCCCCCHHHHHH	34.29	22210691
649	Phosphorylation	MYGNRSNSSMAALIA HCCCCCCHHHHHHHH	25.40	27732954
650	Phosphorylation	YGNRSNSSMAALIAQ CCCCCCHHHHHHHHH	35.25	22210691
658	Phosphorylation	MAALIAQSENNQTDQ HHHHHHHCCCCCCCC	5.56	27732954
679	Phosphorylation	RNLVGRGSSPRGSLS CCCCCCCCCCCCCCC	37.44	23663014
680	Phosphorylation	NLVGRGSSPRGSLSP CCCCCCCCCCCCCCC	30.17	26055452
684	Phosphorylation	RGSSPRGSLSPRSPV CCCCCCCCCCCCCCC	40.10	23927012
686	Phosphorylation	SSPRGSLSPRSPVSS CCCCCCCCCCCCCCE	40.86	23927012
689	Phosphorylation	RGSLSPRSPVSSLQI CCCCCCCCCCCEEEE	38.61	22167270
692	Phosphorylation	LSPRSPVSSLQIRYD CCCCCCCCEEEEEEC	31.25	30266825
693	Phosphorylation	SPRSPVSSLQIRYDQ CCCCCCCEEEEEECC	34.05	23927012
696	Phosphorylation	SPVSSLQIRYDQPGN CCCCEEEEEECCCCC	22.80	18669648
698	Phosphorylation	VSSLQIRYDQPGNSS CCEEEEEECCCCCCC	49.64	27732954
700	Phosphorylation	SLQIRYDQPGNSSLE EEEEEECCCCCCCHH	27.27	20068231
702	Phosphorylation	QIRYDQPGNSSLENL EEEECCCCCCCHHCC	21.69	20068231
704	Phosphorylation	RYDQPGNSSLENLPP EECCCCCCCHHCCCC	50.66	27732954
705	Phosphorylation	YDQPGNSSLENLPPV ECCCCCCCHHCCCCH	32.79	20068231
709	Phosphorylation	GNSSLENLPPVAASI CCCCHHCCCCHHHHH	25.24	20068231
786	Phosphorylation	SVPFPTITANPSPSH CCCCCEEECCCCCCC	36.29	26074081
790	Phosphorylation	PTITANPSPSHQIHT CEEECCCCCCCEEEE	3.00	26074081
792	Phosphorylation	ITANPSPSHQIHTFS EECCCCCCCEEEEEE	16.20	26074081
797	Phosphorylation	SPSHQIHTFSAQTAP CCCCEEEEEECCCCC	26.56	26714015
811	Phosphorylation	PTTDSLNSSKSPHIG CCCCCCCCCCCCCCC	2.50	26074081
812	Phosphorylation	TTDSLNSSKSPHIGN CCCCCCCCCCCCCCC	17.81	26074081
814	Phosphorylation	DSLNSSKSPHIGNSF CCCCCCCCCCCCCCC	3.35	26074081
820	Phosphorylation	KSPHIGNSFLPDNSL CCCCCCCCCCCCCCC	38.94	26074081
1035	O-linked_Glycosylation	TQAPPLHTATTNPFL CCCCCCCCCCCCCCE		OGP
1057	Phosphorylation	SQKVARLSDKTGPVA HHHHHHHHCCCCCCC		26329039
1059	Acetylation	KVARLSDKTGPVAQE HHHHHHCCCCCCCCC		25953088

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of AF10_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of AF10_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of AF10_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TBA1A_RAT	Tuba1a	physical	12750376
YETS4_HUMAN	YEATS4	physical	11756182
SNF5_HUMAN	SMARCB1	physical	11756182
AF10_HUMAN	MLLT10	physical	10860745
DOT1L_HUMAN	DOT1L	physical	15851025

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of AF10_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-705, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-436, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-696; SER-700 ANDSER-702, AND MASS SPECTROMETRY.	18669648 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-406, AND MASSSPECTROMETRY.	17525332 [Abstract]