TBA1A_RAT - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TBA1A_RAT
• UniProt AC: P68370
• Protein Name: Tubulin alpha-1A chain
• Gene Name: Tuba1a
• Organism: Rattus norvegicus (Rat).
• Sequence Length: 451
• Subcellular Localization: Cytoplasm, cytoskeleton.
• Protein Description: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain..
• Protein Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
40	Acetylation	DGQMPSDKTIGGGDD CCCCCCCCCCCCCCC	46.51	25786129
41	Phosphorylation	GQMPSDKTIGGGDDS CCCCCCCCCCCCCCC	29.82	27097102
48	Phosphorylation	TIGGGDDSFNTFFSE CCCCCCCCHHHCCCC	25.74	27097102
51	Phosphorylation	GGDDSFNTFFSETGA CCCCCHHHCCCCCCC	24.85	27097102
54	Phosphorylation	DSFNTFFSETGAGKH CCHHHCCCCCCCCCC	30.02	23984901
56	Phosphorylation	FNTFFSETGAGKHVP HHHCCCCCCCCCCCC	31.00	23984901
60	Ubiquitination	FSETGAGKHVPRAVF CCCCCCCCCCCEEEE	40.68	-
60	"N6,N6-dimethyllysine"	FSETGAGKHVPRAVF CCCCCCCCCCCEEEE	40.68	-
60	Methylation	FSETGAGKHVPRAVF CCCCCCCCCCCEEEE	40.68	-
60	Acetylation	FSETGAGKHVPRAVF CCCCCCCCCCCEEEE	40.68	22902405
61	Methylation	SETGAGKHVPRAVFV CCCCCCCCCCEEEEE	33.62	4207429
64	Methylation	GAGKHVPRAVFVDLE CCCCCCCEEEEECCC	42.15	4207423
64	Dimethylation	GAGKHVPRAVFVDLE CCCCCCCEEEEECCC	42.15	-
80	Phosphorylation	TVIDEVRTGTYRQLF EEEHHCCCCCCHHCC	38.77	23984901
82	Phosphorylation	IDEVRTGTYRQLFHP EHHCCCCCCHHCCCH	18.21	23984901
83	Phosphorylation	DEVRTGTYRQLFHPE HHCCCCCCHHCCCHH	9.56	23984901
96	Ubiquitination	PEQLITGKEDAANNY HHHHCCCCHHHHHHC	43.71	-
96	Acetylation	PEQLITGKEDAANNY HHHHCCCCHHHHHHC	43.71	22902405
103	Phosphorylation	KEDAANNYARGHYTI CHHHHHHCCCCCCCC	9.48	27097102
103	Nitration	KEDAANNYARGHYTI CHHHHHHCCCCCCCC	9.48	-
108	Phosphorylation	NNYARGHYTIGKEII HHCCCCCCCCCHHHH	11.68	27097102
109	Phosphorylation	NYARGHYTIGKEIID HCCCCCCCCCHHHHH	19.99	27097102
140	Phosphorylation	QGFLVFHSFGGGTGS CCEEEEEECCCCCCC	17.70	30240740
158	Phosphorylation	SLLMERLSVDYGKKS HHHHHHHCCCCCCCC	21.11	30240740
163	Acetylation	RLSVDYGKKSKLEFS HHCCCCCCCCCEEEE	47.87	13667149
163	Ubiquitination	RLSVDYGKKSKLEFS HHCCCCCCCCCEEEE	47.87	-
164	Ubiquitination	LSVDYGKKSKLEFSI HCCCCCCCCCEEEEE	48.75	-
210	Phosphorylation	MVDNEAIYDICRRNL EECHHHHHHHHHCCC	13.30	-
223	Phosphorylation	NLDIERPTYTNLNRL CCCCCCCCCCCHHHH	49.55	27097102
224	Phosphorylation	LDIERPTYTNLNRLI CCCCCCCCCCHHHHH	9.05	27097102
225	Phosphorylation	DIERPTYTNLNRLIG CCCCCCCCCHHHHHH	35.39	27097102
271	Phosphorylation	RIHFPLATYAPVISA CCCCCCCCCCCCCCH	28.09	30240740
272	Phosphorylation	IHFPLATYAPVISAE CCCCCCCCCCCCCHH	11.87	-
277	Phosphorylation	ATYAPVISAEKAYHE CCCCCCCCHHHHHHH	30.52	23984901
282	Phosphorylation	VISAEKAYHEQLSVA CCCHHHHHHHHCCHH	19.56	23984901
282	Nitrated tyrosine	VISAEKAYHEQLSVA CCCHHHHHHHHCCHH	19.56	-
282	Nitration	VISAEKAYHEQLSVA CCCHHHHHHHHCCHH	19.56	-
287	Phosphorylation	KAYHEQLSVAEITNA HHHHHHCCHHHHHHH	20.98	23984901
292	Phosphorylation	QLSVAEITNACFEPA HCCHHHHHHHHCCCH	14.31	23984901
295	S-nitrosylation	VAEITNACFEPANQM HHHHHHHHCCCHHHC	4.27	22178444
295	S-nitrosocysteine	VAEITNACFEPANQM HHHHHHHHCCCHHHC	4.27	-
304	Ubiquitination	EPANQMVKCDPRHGK CCHHHCCCCCCCCCC	27.81	-
311	Ubiquitination	KCDPRHGKYMACCLL CCCCCCCCEEEEHHH	26.23	-
312	Phosphorylation	CDPRHGKYMACCLLY CCCCCCCEEEEHHHC	8.44	-
315	S-nitrosylation	RHGKYMACCLLYRGD CCCCEEEEHHHCCCC	0.67	22178444
315	S-nitrosocysteine	RHGKYMACCLLYRGD CCCCEEEEHHHCCCC	0.67	-
316	S-nitrosylation	HGKYMACCLLYRGDV CCCEEEEHHHCCCCC	1.81	22178444
316	S-nitrosocysteine	HGKYMACCLLYRGDV CCCEEEEHHHCCCCC	1.81	-
319	Phosphorylation	YMACCLLYRGDVVPK EEEEHHHCCCCCCCC	10.29	-
326	Ubiquitination	YRGDVVPKDVNAAIA CCCCCCCCCCHHHHH	63.80	-
326	Acetylation	YRGDVVPKDVNAAIA CCCCCCCCCCHHHHH	63.80	22902405
334	Phosphorylation	DVNAAIATIKTKRTI CCHHHHHHCCCCCEE	19.52	27097102
336	Ubiquitination	NAAIATIKTKRTIQF HHHHHHCCCCCEEEE	43.57	-
336	Acetylation	NAAIATIKTKRTIQF HHHHHHCCCCCEEEE	43.57	14497325
337	Phosphorylation	AAIATIKTKRTIQFV HHHHHCCCCCEEEEE	23.16	23984901
338	Ubiquitination	AIATIKTKRTIQFVD HHHHCCCCCEEEEEE	42.41	-
340	Phosphorylation	ATIKTKRTIQFVDWC HHCCCCCEEEEEEEC	22.23	23984901
347	S-nitrosylation	TIQFVDWCPTGFKVG EEEEEEECCCCEEEE	1.55	22178444
347	S-nitrosocysteine	TIQFVDWCPTGFKVG EEEEEEECCCCEEEE	1.55	-
352	Ubiquitination	DWCPTGFKVGINYQP EECCCCEEEEEECCC	40.68	-
357	Phosphorylation	GFKVGINYQPPTVVP CEEEEEECCCCEECC	22.07	23984901
361	Phosphorylation	GINYQPPTVVPGGDL EEECCCCEECCCCCH	40.60	23984901
370	Ubiquitination	VPGGDLAKVQRAVCM CCCCCHHHHHHHHHH	46.31	-
370	Acetylation	VPGGDLAKVQRAVCM CCCCCHHHHHHHHHH	46.31	25786129
376	S-nitrosocysteine	AKVQRAVCMLSNTTA HHHHHHHHHHCCHHH	1.90	-
376	S-nitrosylation	AKVQRAVCMLSNTTA HHHHHHHHHHCCHHH	1.90	22178444
394	Ubiquitination	AWARLDHKFDLMYAK HHHHCCCCCCHHHEE	39.99	-
394	Acetylation	AWARLDHKFDLMYAK HHHHCCCCCCHHHEE	39.99	22902405
399	Phosphorylation	DHKFDLMYAKRAFVH CCCCCHHHEEEHHHH	19.10	-
401	Ubiquitination	KFDLMYAKRAFVHWY CCCHHHEEEHHHHHH	27.11	-
401	Acetylation	KFDLMYAKRAFVHWY CCCHHHEEEHHHHHH	27.11	22902405
408	Phosphorylation	KRAFVHWYVGEGMEE EEHHHHHHCCCCCCC	5.45	25403869
432	Phosphorylation	MAALEKDYEEVGVDS HHHHHHCHHHHCCCC	25.67	28689409
439	Phosphorylation	YEEVGVDSVEGEGEE HHHHCCCCCCCCCCC	21.47	29779826
445	5-glutamyl polyglutamate	DSVEGEGEEEGEEY- CCCCCCCCCCCCCC-	48.88	-
445	Formation of an isopeptide bond	DSVEGEGEEEGEEY- CCCCCCCCCCCCCC-	48.88	-
451	Nitration	GEEEGEEY------- CCCCCCCC-------	21.39	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TBA1A_RAT !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
40	K	Acetylation	Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen.	-
40	K	Methylation	Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.	-

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TBA1A_RAT !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
EPN1_RAT	Epn1	physical	12750376
EPN2_RAT	Epn2	physical	12750376
GRM7_RAT	Grm7	physical	11953448
ATX3_RAT	Atxn3	physical	19666135
HDAC6_HUMAN	HDAC6	physical	19961433
DPYL2_RAT	Dpysl2	physical	12134159

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.