dbPTM

MDHC_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MDHC_HUMAN
UniProt AC	P40925
Protein Name	Malate dehydrogenase, cytoplasmic
Gene Name	MDH1
Organism	Homo sapiens (Human).
Sequence Length	334
Subcellular Localization	Cytoplasm.
Protein Description
Protein Sequence	MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSEPIRVLV ------CCCCEEEEE	52.67	25944712
5 (in isoform 3)	Phosphorylation	-	4.11	26437602
14	Acetylation	VLVTGAAGQIAYSLL EEEECCHHHHHHHHH	20.92	19608861
22	Phosphorylation	QIAYSLLYSIGNGSV HHHHHHHHHCCCCCC	11.97	22673903
23	Phosphorylation	IAYSLLYSIGNGSVF HHHHHHHHCCCCCCC	24.80	22673903
29	Acetylation	YSIGNGSVFGKDQPI HHCCCCCCCCCCCCE	8.54	19608861
29	Ubiquitination	YSIGNGSVFGKDQPI HHCCCCCCCCCCCCE	8.54	19608861
44	Phosphorylation	ILVLLDITPMMGVLD EEEEEECHHHCCHHH	12.56	24043423
71	Phosphorylation	LLKDVIATDKEDVAF HHHHHHCCCHHHCCC	36.16	23911959
73	Acetylation	KDVIATDKEDVAFKD HHHHCCCHHHCCCCC	52.00	23954790
73	Ubiquitination	KDVIATDKEDVAFKD HHHHCCCHHHCCCCC	52.00	21906983
89	Phosphorylation	DVAILVGSMPRREGM CEEEEECCCCCCCCC	20.38	22673903
90	Sulfoxidation	VAILVGSMPRREGME EEEEECCCCCCCCCC	2.17	21406390
103	Acetylation	MERKDLLKANVKIFK CCHHHHHHHHHHHHH	45.25	19608861
103	Succinylation	MERKDLLKANVKIFK CCHHHHHHHHHHHHH	45.25	23954790
103	Ubiquitination	MERKDLLKANVKIFK CCHHHHHHHHHHHHH	45.25	19608861
107	Acetylation	DLLKANVKIFKSQGA HHHHHHHHHHHHCCC	42.08	23749302
107	Succinylation	DLLKANVKIFKSQGA HHHHHHHHHHHHCCC	42.08	23954790
107	Ubiquitination	DLLKANVKIFKSQGA HHHHHHHHHHHHCCC	42.08	-
107 (in isoform 3)	Phosphorylation	-	42.08	27251275
110	Acetylation	KANVKIFKSQGAALD HHHHHHHHHCCCHHH	45.39	25953088
110	Malonylation	KANVKIFKSQGAALD HHHHHHHHHCCCHHH	45.39	26320211
110	Methylation	KANVKIFKSQGAALD HHHHHHHHHCCCHHH	45.39	38025153
110	Succinylation	KANVKIFKSQGAALD HHHHHHHHHCCCHHH	45.39	-
110	Succinylation	KANVKIFKSQGAALD HHHHHHHHHCCCHHH	45.39	27452117
110	Ubiquitination	KANVKIFKSQGAALD HHHHHHHHHCCCHHH	45.39	-
111	Phosphorylation	ANVKIFKSQGAALDK HHHHHHHHCCCHHHH	24.09	26657352
118	Acetylation	SQGAALDKYAKKSVK HCCCHHHHHCCCCCE	48.21	22693256
118	Ubiquitination	SQGAALDKYAKKSVK HCCCHHHHHCCCCCE	48.21	22693256
119	Phosphorylation	QGAALDKYAKKSVKV CCCHHHHHCCCCCEE	24.26	28152594
121	Acetylation	AALDKYAKKSVKVIV CHHHHHCCCCCEEEE	42.35	22693256
121	Ubiquitination	AALDKYAKKSVKVIV CHHHHHCCCCCEEEE	42.35	-
122	Ubiquitination	ALDKYAKKSVKVIVV HHHHHCCCCCEEEEE	52.92	-
125	Ubiquitination	KYAKKSVKVIVVGNP HHCCCCCEEEEECCC	33.08	21890473
129 (in isoform 3)	Phosphorylation	-	4.16	24719451
135	Phosphorylation	VVGNPANTNCLTASK EECCCCCCCCCCCCC	29.43	28348404
136	Ubiquitination	VGNPANTNCLTASKS ECCCCCCCCCCCCCC	20.71	21890473
136	Ubiquitination	VGNPANTNCLTASKS ECCCCCCCCCCCCCC	20.71	21890473
136	Acetylation	VGNPANTNCLTASKS ECCCCCCCCCCCCCC	20.71	19608861
136	Ubiquitination	VGNPANTNCLTASKS ECCCCCCCCCCCCCC	20.71	19608861
137	S-nitrosocysteine	GNPANTNCLTASKSA CCCCCCCCCCCCCCC	3.17	-
137	S-nitrosylation	GNPANTNCLTASKSA CCCCCCCCCCCCCCC	3.17	18335467
137	S-palmitoylation	GNPANTNCLTASKSA CCCCCCCCCCCCCCC	3.17	26865113
139	Phosphorylation	PANTNCLTASKSAPS CCCCCCCCCCCCCCC	30.96	28348404
141	Phosphorylation	NTNCLTASKSAPSIP CCCCCCCCCCCCCCC	23.16	29507054
142	Acetylation	TNCLTASKSAPSIPK CCCCCCCCCCCCCCC	48.44	26051181
142	Ubiquitination	TNCLTASKSAPSIPK CCCCCCCCCCCCCCC	48.44	-
143	Ubiquitination	NCLTASKSAPSIPKE CCCCCCCCCCCCCCC	42.80	21890473
143	Ubiquitination	NCLTASKSAPSIPKE CCCCCCCCCCCCCCC	42.80	21890473
143	Phosphorylation	NCLTASKSAPSIPKE CCCCCCCCCCCCCCC	42.80	26437602
143	Ubiquitination	NCLTASKSAPSIPKE CCCCCCCCCCCCCCC	42.80	21890473
146	Phosphorylation	TASKSAPSIPKENFS CCCCCCCCCCCCCCC	51.76	28188228
149	Acetylation	KSAPSIPKENFSCLT CCCCCCCCCCCCHHH	64.72	26051181
149	Ubiquitination	KSAPSIPKENFSCLT CCCCCCCCCCCCHHH	64.72	-
153	Phosphorylation	SIPKENFSCLTRLDH CCCCCCCCHHHHCCC	21.57	26437602
154	S-nitrosylation	IPKENFSCLTRLDHN CCCCCCCHHHHCCCH	3.83	24105792
157 (in isoform 3)	Phosphorylation	-	25.77	27251275
164	Ubiquitination	RLDHNRAKAQIALKL HCCCHHHHHHHHHHH	37.27	21890473
170	Ubiquitination	AKAQIALKLGVTAND HHHHHHHHHCCCHHH	33.89	-
174	Phosphorylation	IALKLGVTANDVKNV HHHHHCCCHHHCCEE	20.25	22673903
179	Acetylation	GVTANDVKNVIIWGN CCCHHHCCEEEEECC	48.65	25953088
179	Ubiquitination	GVTANDVKNVIIWGN CCCHHHCCEEEEECC	48.65	-
188	Phosphorylation	VIIWGNHSSTQYPDV EEEECCCCCCCCCCC	38.80	23401153
189	Phosphorylation	IIWGNHSSTQYPDVN EEECCCCCCCCCCCC	16.39	28152594
190	Phosphorylation	IWGNHSSTQYPDVNH EECCCCCCCCCCCCC	34.83	28152594
192	Phosphorylation	GNHSSTQYPDVNHAK CCCCCCCCCCCCCCE	11.07	28152594
199	Acetylation	YPDVNHAKVKLQGKE CCCCCCCEEEECCEE	32.14	25953088
199	Ubiquitination	YPDVNHAKVKLQGKE CCCCCCCEEEECCEE	32.14	-
201	Ubiquitination	DVNHAKVKLQGKEVG CCCCCEEEECCEEEE	34.09	-
205	Acetylation	AKVKLQGKEVGVYEA CEEEECCEEEEEEEE	35.94	26051181
205	Ubiquitination	AKVKLQGKEVGVYEA CEEEECCEEEEEEEE	35.94	21890473
207 (in isoform 3)	Phosphorylation	-	7.77	24719451
208 (in isoform 3)	Phosphorylation	-	19.85	27251275
209	Acetylation	LQGKEVGVYEALKDD ECCEEEEEEEECCCC	4.76	19608861
209	Ubiquitination	LQGKEVGVYEALKDD ECCEEEEEEEECCCC	4.76	19608861
210	Phosphorylation	QGKEVGVYEALKDDS CCEEEEEEEECCCCC	6.91	25159151
214	Acetylation	VGVYEALKDDSWLKG EEEEEECCCCCCCCC	67.54	26822725
214	Succinylation	VGVYEALKDDSWLKG EEEEEECCCCCCCCC	67.54	-
214	Succinylation	VGVYEALKDDSWLKG EEEEEECCCCCCCCC	67.54	23954790
214	Ubiquitination	VGVYEALKDDSWLKG EEEEEECCCCCCCCC	67.54	21890473
217	Phosphorylation	YEALKDDSWLKGEFV EEECCCCCCCCCCEE	44.43	28857561
220	Ubiquitination	LKDDSWLKGEFVTTV CCCCCCCCCCEEEEH	50.57	21890473
223	Ubiquitination	DSWLKGEFVTTVQQR CCCCCCCEEEEHHHH	8.90	21890473
223	Ubiquitination	DSWLKGEFVTTVQQR CCCCCCCEEEEHHHH	8.90	21890473
223	Ubiquitination	DSWLKGEFVTTVQQR CCCCCCCEEEEHHHH	8.90	21890473
228 (in isoform 3)	Phosphorylation	-	25.76	27642862
230	Methylation	FVTTVQQRGAAVIKA EEEEHHHHCHHHHHH	21.45	58858501
232	Ubiquitination	TTVQQRGAAVIKARK EEHHHHCHHHHHHHH	10.76	21890473
232	Ubiquitination	TTVQQRGAAVIKARK EEHHHHCHHHHHHHH	10.76	21890473
232	Ubiquitination	TTVQQRGAAVIKARK EEHHHHCHHHHHHHH	10.76	21890473
235 (in isoform 3)	Phosphorylation	-	2.18	27251275
236	Acetylation	QRGAAVIKARKLSSA HHCHHHHHHHHHHHH	35.22	25953088
236	Ubiquitination	QRGAAVIKARKLSSA HHCHHHHHHHHHHHH	35.22	-
239	Acetylation	AAVIKARKLSSAMSA HHHHHHHHHHHHHHH	58.54	25953088
239	Ubiquitination	AAVIKARKLSSAMSA HHHHHHHHHHHHHHH	58.54	-
241	Phosphorylation	VIKARKLSSAMSAAK HHHHHHHHHHHHHHH	21.06	29255136
242	Phosphorylation	IKARKLSSAMSAAKA HHHHHHHHHHHHHHH	38.13	30266825
244	Sulfoxidation	ARKLSSAMSAAKAIC HHHHHHHHHHHHHHH	2.79	30846556
245	Phosphorylation	RKLSSAMSAAKAICD HHHHHHHHHHHHHHH	25.45	30266825
248	Acetylation	SSAMSAAKAICDHVR HHHHHHHHHHHHHHH	37.69	25953088
259 (in isoform 3)	Phosphorylation	-	8.79	24719451
277	Phosphorylation	GVISDGNSYGVPDDL EEECCCCCCCCCCCC	28.88	30576142
278	Phosphorylation	VISDGNSYGVPDDLL EECCCCCCCCCCCCE	26.58	30576142
296	Phosphorylation	PVVIKNKTWKFVEGL CEEEECCCEEEECCC	43.19	22673903
298	Acetylation	VIKNKTWKFVEGLPI EEECCCEEEECCCCC	44.77	22693256
298	Succinylation	VIKNKTWKFVEGLPI EEECCCEEEECCCCC	44.77	-
298	Succinylation	VIKNKTWKFVEGLPI EEECCCEEEECCCCC	44.77	19608861
298	Ubiquitination	VIKNKTWKFVEGLPI EEECCCEEEECCCCC	44.77	22693256
309	Phosphorylation	GLPINDFSREKMDLT CCCCCCCCHHHCCCC	42.25	21712546
312	Acetylation	INDFSREKMDLTAKE CCCCCHHHCCCCHHH	36.28	25953088
312	Ubiquitination	INDFSREKMDLTAKE CCCCCHHHCCCCHHH	36.28	-
316	Ubiquitination	SREKMDLTAKELTEE CHHHCCCCHHHHHHH	31.27	21890473
316	Ubiquitination	SREKMDLTAKELTEE CHHHCCCCHHHHHHH	31.27	21890473
316	Acetylation	SREKMDLTAKELTEE CHHHCCCCHHHHHHH	31.27	19608861
316	Phosphorylation	SREKMDLTAKELTEE CHHHCCCCHHHHHHH	31.27	28634298
316	Ubiquitination	SREKMDLTAKELTEE CHHHCCCCHHHHHHH	31.27	19608861
318	Succinylation	EKMDLTAKELTEEKE HHCCCCHHHHHHHHH	48.28	-
318	Succinylation	EKMDLTAKELTEEKE HHCCCCHHHHHHHHH	48.28	23954790
318	Ubiquitination	EKMDLTAKELTEEKE HHCCCCHHHHHHHHH	48.28	21890473
321	Phosphorylation	DLTAKELTEEKESAF CCCHHHHHHHHHHHH	43.09	21712546
324	Ubiquitination	AKELTEEKESAFEFL HHHHHHHHHHHHHHH	51.22	21890473
326	Phosphorylation	ELTEEKESAFEFLSS HHHHHHHHHHHHHHC	49.69	28176443
332	Phosphorylation	ESAFEFLSSA----- HHHHHHHHCC-----	29.55	30108239
333	Phosphorylation	SAFEFLSSA------ HHHHHHHCC------	39.90	28176443
336	Ubiquitination	EFLSSA--------- HHHHCC---------		21890473
336	Ubiquitination	EFLSSA--------- HHHHCC---------		21890473
350 (in isoform 3)	Phosphorylation	-		27251275
351 (in isoform 3)	Phosphorylation	-		24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of MDHC_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
118	K	Acetylation		19608861
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MDHC_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
P53_HUMAN	TP53	physical	19229245
PRDX6_HUMAN	PRDX6	physical	22939629
NHRF1_HUMAN	SLC9A3R1	physical	22939629
PRDX5_HUMAN	PRDX5	physical	22939629
PGM1_HUMAN	PGM1	physical	22939629
ESTD_HUMAN	ESD	physical	26344197
GLRX1_HUMAN	GLRX	physical	26344197
AATC_HUMAN	GOT1	physical	26344197
PDXK_HUMAN	PDXK	physical	26344197
PGM1_HUMAN	PGM1	physical	26344197
PGM2_HUMAN	PGM2	physical	26344197
SCPDL_HUMAN	SCCPDH	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MDHC_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-103; LYS-118 AND LYS-298,AND MASS SPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-241, AND MASSSPECTROMETRY.	19413330 [Abstract]

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