RPB2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RPB2_HUMAN
• UniProt AC: P30876
• Protein Name: DNA-directed RNA polymerase II subunit RPB2
• Gene Name: POLR2B
• Organism: Homo sapiens (Human).
• Sequence Length: 1174
• Subcellular Localization: Nucleus .
• Protein Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB2 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template (By similarity)..
• Protein Sequence: MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
37	Ubiquitination	ISSYFDEKGLVRQQL HHHHCCCCCCHHHHH	60.43	-
75	Phosphorylation	QAEAQHASGEVEEPP HHHHHHHCCCCCCCC	33.10	25159151
87	Ubiquitination	EPPRYLLKFEQIYLS CCCCEEEEEEEEECC	44.23	21890473
92	Phosphorylation	LLKFEQIYLSKPTHW EEEEEEEECCCCCCC	12.48	28152594
94	Phosphorylation	KFEQIYLSKPTHWER EEEEEECCCCCCCCC	21.44	28152594
95	Acetylation	FEQIYLSKPTHWERD EEEEECCCCCCCCCC	51.87	26051181
95	Ubiquitination	FEQIYLSKPTHWERD EEEEECCCCCCCCCC	51.87	21890473
97	Phosphorylation	QIYLSKPTHWERDGA EEECCCCCCCCCCCC	43.27	28152594
106	Phosphorylation	WERDGAPSPMMPNEA CCCCCCCCCCCCCHH	25.54	21815630
119	Phosphorylation	EARLRNLTYSAPLYV HHHHCCCCCCCCEEE	20.98	28152594
120	Phosphorylation	ARLRNLTYSAPLYVD HHHCCCCCCCCEEEE	13.15	28152594
121	Phosphorylation	RLRNLTYSAPLYVDI HHCCCCCCCCEEEEE	20.41	28152594
125	Phosphorylation	LTYSAPLYVDITKTV CCCCCCEEEEEEEHH	8.60	27642862
130	Ubiquitination	PLYVDITKTVIKEGE CEEEEEEEHHHHHHH	40.92	21890473
134	Acetylation	DITKTVIKEGEEQLQ EEEEHHHHHHHHHHH	56.16	26051181
134	Ubiquitination	DITKTVIKEGEEQLQ EEEEHHHHHHHHHHH	56.16	21890473
146	Ubiquitination	QLQTQHQKTFIGKIP HHHHHHHHHHHCHHH	43.19	21890473
147	Phosphorylation	LQTQHQKTFIGKIPI HHHHHHHHHHCHHHH	17.57	20860994
151	Acetylation	HQKTFIGKIPIMLRS HHHHHHCHHHHHHHH	40.26	25953088
151	Ubiquitination	HQKTFIGKIPIMLRS HHHHHHCHHHHHHHH	40.26	21890473
199	Ubiquitination	KVLIAQEKMATNTVY EEEEEEEECCCCEEE	23.04	21890473
202	Phosphorylation	IAQEKMATNTVYVFA EEEEECCCCEEEEEE	28.11	21406692
204	Phosphorylation	QEKMATNTVYVFAKK EEECCCCEEEEEEEC	14.62	21406692
206	Phosphorylation	KMATNTVYVFAKKDS ECCCCEEEEEEECCC	6.44	21406692
210	Ubiquitination	NTVYVFAKKDSKYAY CEEEEEEECCCCCEE	45.72	21890473
211	Ubiquitination	TVYVFAKKDSKYAYT EEEEEEECCCCCEEE	64.90	-
213	Phosphorylation	YVFAKKDSKYAYTGE EEEEECCCCCEEECC	36.12	20068231
214	Ubiquitination	VFAKKDSKYAYTGEC EEEECCCCCEEECCC	43.99	-
215	Phosphorylation	FAKKDSKYAYTGECR EEECCCCCEEECCCH	14.59	20068231
217	Phosphorylation	KKDSKYAYTGECRSC ECCCCCEEECCCHHH	16.52	20068231
218	Phosphorylation	KDSKYAYTGECRSCL CCCCCEEECCCHHHH	20.52	20068231
228	Phosphorylation	CRSCLENSSRPTSTI CHHHHHCCCCCCCCH	20.53	22210691
233	Phosphorylation	ENSSRPTSTIWVSML HCCCCCCCCHHHHHH	22.15	22210691
234	Phosphorylation	NSSRPTSTIWVSMLA CCCCCCCCHHHHHHH	22.72	22210691
248	Ubiquitination	ARGGQGAKKSAIGQR HCCCCCCCHHHHCCH	54.83	21890473
249	Ubiquitination	RGGQGAKKSAIGQRI CCCCCCCHHHHCCHH	44.58	21890473
264	Ubiquitination	VATLPYIKQEVPIII HHCHHHHCCCCCEEE	34.26	21890473
337	Ubiquitination	VTKEKRIKYAKEVLQ CCHHHHHHHHHHHHH	43.09	-
340	Ubiquitination	EKRIKYAKEVLQKEM HHHHHHHHHHHHHHC	45.31	21890473
345	Ubiquitination	YAKEVLQKEMLPHVG HHHHHHHHHCCCCCC	40.71	-
354	Phosphorylation	MLPHVGVSDFCETKK CCCCCCHHHHHHHCH	21.02	-
359	Phosphorylation	GVSDFCETKKAYFLG CHHHHHHHCHHHHHH	38.74	-
360	Acetylation	VSDFCETKKAYFLGY HHHHHHHCHHHHHHH	17.08	26051181
360	Ubiquitination	VSDFCETKKAYFLGY HHHHHHHCHHHHHHH	17.08	-
361	Ubiquitination	SDFCETKKAYFLGYM HHHHHHCHHHHHHHH	56.59	-
391	Ubiquitination	DRDHYGNKRLDLAGP CCCCCCCCCHHHHHH	50.39	21890473
413	Ubiquitination	GMFKNLLKEVRIYAQ HHHHHHHHHHHHHHH	58.28	21890473
421	Ubiquitination	EVRIYAQKFIDRGKD HHHHHHHHHHHCCCC	35.79	21890473
427	Ubiquitination	QKFIDRGKDFNLELA HHHHHCCCCCCCCHH	61.10	21890473
436	Ubiquitination	FNLELAIKTRIISDG CCCCHHEEEEEECCC	26.58	21890473
437	Phosphorylation	NLELAIKTRIISDGL CCCHHEEEEEECCCC	22.46	-
441	Phosphorylation	AIKTRIISDGLKYSL HEEEEEECCCCCEEE	24.21	28176443
445	Methylation	RIISDGLKYSLATGN EEECCCCCEEECCCC	38.02	42363977
445	Ubiquitination	RIISDGLKYSLATGN EEECCCCCEEECCCC	38.02	21890473
457	Ubiquitination	TGNWGDQKKAHQARA CCCCCCHHHHHHHHH	57.58	21890473
458	Acetylation	GNWGDQKKAHQARAG CCCCCHHHHHHHHHH	45.53	24468083
458	Ubiquitination	GNWGDQKKAHQARAG CCCCCHHHHHHHHHH	45.53	-
467	Phosphorylation	HQARAGVSQVLNRLT HHHHHHHHHHHHHHH	17.41	24670416
487	Phosphorylation	SHLRRLNSPIGRDGK HHHHHHCCCCCCCCC	23.61	30266825
494	Ubiquitination	SPIGRDGKLAKPRQL CCCCCCCCCCCHHHC	49.95	-
497	Ubiquitination	GRDGKLAKPRQLHNT CCCCCCCCHHHCCCC	51.69	-
578	Acetylation	GCWVGIHKDPEQLMN CEEEECCCCHHHHHH	73.40	26051181
578	Ubiquitination	GCWVGIHKDPEQLMN CEEEECCCCHHHHHH	73.40	-
586	Phosphorylation	DPEQLMNTLRKLRRQ CHHHHHHHHHHHHHH	17.61	20860994
630	Ubiquitination	RPLLIVEKQKLLLKK CEEHHHHHHHHHHHH	41.67	21890473
632	Ubiquitination	LLIVEKQKLLLKKRH EHHHHHHHHHHHHHH	52.77	-
637	Ubiquitination	KQKLLLKKRHIDQLK HHHHHHHHHHHHHHH	49.53	-
644	2-Hydroxyisobutyrylation	KRHIDQLKEREYNNY HHHHHHHHHHHCCCC	49.44	-
644	Ubiquitination	KRHIDQLKEREYNNY HHHHHHHHHHHCCCC	49.44	21890473
689	Phosphorylation	LQEKEVAYCSTYTHC HHHCHHEECCCCCCC	7.66	22817900
724	Phosphorylation	NQSPRNTYQSAMGKQ CCCCCHHHHHHHHHH	12.20	-
730	Methylation	TYQSAMGKQAMGVYI HHHHHHHHHHCEEEE	23.07	24785225
730	Ubiquitination	TYQSAMGKQAMGVYI HHHHHHHHHHCEEEE	23.07	21890473
736	Phosphorylation	GKQAMGVYITNFHVR HHHHCEEEEEEEEEE	9.04	-
746	Phosphorylation	NFHVRMDTLAHVLYY EEEEECHHHHHHHHC	19.26	28270605
752	Phosphorylation	DTLAHVLYYPQKPLV HHHHHHHHCCCCCCE	16.41	28270605
753	Phosphorylation	TLAHVLYYPQKPLVT HHHHHHHCCCCCCEE	8.60	28270605
756	Ubiquitination	HVLYYPQKPLVTTRS HHHHCCCCCCEECCC	35.50	21890473
760	Phosphorylation	YPQKPLVTTRSMEYL CCCCCCEECCCHHHH	24.95	28270605
761	Phosphorylation	PQKPLVTTRSMEYLR CCCCCEECCCHHHHH	17.17	28270605
763	Phosphorylation	KPLVTTRSMEYLRFR CCCEECCCHHHHHHC	17.55	23186163
803	Methylation	MNRSAVDRGFFRSVF ECHHHHHHHHHHHHH	36.90	115488157
815	Acetylation	SVFYRSYKEQESKKG HHHHHHHHHHHHCCC	54.92	20167786
815	Ubiquitination	SVFYRSYKEQESKKG HHHHHHHHHHHHCCC	54.92	21890473
820	Acetylation	SYKEQESKKGFDQEE HHHHHHHCCCCCHHH	57.05	20167786
821	Ubiquitination	YKEQESKKGFDQEEV HHHHHHCCCCCHHHH	74.27	21890473
831	Acetylation	DQEEVFEKPTRETCQ CHHHHHCCCCHHHHH	39.98	26051181
831	Ubiquitination	DQEEVFEKPTRETCQ CHHHHHCCCCHHHHH	39.98	21890473
845	Phosphorylation	QGMRHAIYDKLDDDG HHHHHHHHHCCCCCC	14.07	28796482
847	Acetylation	MRHAIYDKLDDDGLI HHHHHHHCCCCCCCC	36.52	26051181
847	Ubiquitination	MRHAIYDKLDDDGLI HHHHHHHCCCCCCCC	36.52	21890473
869	Ubiquitination	GDDVIIGKTVTLPEN CCCEEEECEEECCCC	29.43	21890473
870	Phosphorylation	DDVIIGKTVTLPENE CCEEEECEEECCCCH	17.32	-
872	Phosphorylation	VIIGKTVTLPENEDE EEEECEEECCCCHHH	41.65	-
889	Ubiquitination	STNRRYTKRDCSTFL HHCCCCCHHHHHHHH	36.99	-
917	Ubiquitination	TLNQEGYKFCKIRVR EECCCCCEEEEEEEE	55.96	-
920	Ubiquitination	QEGYKFCKIRVRSVR CCCCEEEEEEEEEEE	36.36	-
925	Phosphorylation	FCKIRVRSVRIPQIG EEEEEEEEEECCCCC	16.94	28555341
934	Acetylation	RIPQIGDKFASRHGQ ECCCCCHHHHHHHCC	37.30	26051181
934	Ubiquitination	RIPQIGDKFASRHGQ ECCCCCHHHHHHHCC	37.30	21890473
937	Phosphorylation	QIGDKFASRHGQKGT CCCHHHHHHHCCCCC	28.13	28857561
942	Ubiquitination	FASRHGQKGTCGIQY HHHHHCCCCCCEEEE	61.86	-
988	Ubiquitination	LIECLQGKVSANKGE HHHHHHCCCCCCCCC	21.76	-
993	Acetylation	QGKVSANKGEIGDAT HCCCCCCCCCCCCCC	58.60	26051181
993	Sumoylation	QGKVSANKGEIGDAT HCCCCCCCCCCCCCC	58.60	-
993	Ubiquitination	QGKVSANKGEIGDAT HCCCCCCCCCCCCCC	58.60	21890473
1000	Phosphorylation	KGEIGDATPFNDAVN CCCCCCCCCCCCCCC	32.90	28555341
1010	Ubiquitination	NDAVNVQKISNLLSD CCCCCHHHHHHHHHH	43.76	21890473
1012	Phosphorylation	AVNVQKISNLLSDYG CCCHHHHHHHHHHCC	28.58	23663014
1016	Phosphorylation	QKISNLLSDYGYHLR HHHHHHHHHCCCCCC	31.77	23663014
1018	Phosphorylation	ISNLLSDYGYHLRGN HHHHHHHCCCCCCCC	19.06	23663014
1020	Phosphorylation	NLLSDYGYHLRGNEV HHHHHCCCCCCCCEE	7.46	23663014
1023	Methylation	SDYGYHLRGNEVLYN HHCCCCCCCCEEEEC	32.36	115488149
1029	Phosphorylation	LRGNEVLYNGFTGRK CCCCEEEECCCCCCE	20.58	28152594
1033	Phosphorylation	EVLYNGFTGRKITSQ EEEECCCCCCEECEE	37.16	28152594
1036	Ubiquitination	YNGFTGRKITSQIFI ECCCCCCEECEEEEE	52.24	21890473
1046	O-linked_Glycosylation	SQIFIGPTYYQRLKH EEEEECCHHHHHHHH	29.64	30379171
1052	Methylation	PTYYQRLKHMVDDKI CHHHHHHHHHHCHHH	31.66	24129315
1058	Acetylation	LKHMVDDKIHSRARG HHHHHCHHHHHCCCC	37.54	25953088
1058	Ubiquitination	LKHMVDDKIHSRARG HHHHHCHHHHHCCCC	37.54	21890473
1064	Methylation	DKIHSRARGPIQILN HHHHHCCCCCCEEEC	50.76	54558109
1079	Phosphorylation	RQPMEGRSRDGGLRF CCCCCCCCCCCCCCC	45.65	29457462
1143	Ubiquitination	ECRGCRNKTQISLVR ECCCCCCCCCEEEEE	23.58	21890473
1144	Phosphorylation	CRGCRNKTQISLVRM CCCCCCCCCEEEEEH	34.38	20068231
1147	Phosphorylation	CRNKTQISLVRMPYA CCCCCCEEEEEHHHH	15.82	20068231
1166	Phosphorylation	FQELMSMSIAPRMMS HHHHHHCCCCHHCCC	14.32	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RPB2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RPB2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RPB2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RPB3_HUMAN	POLR2C	physical	9201987
RPAB1_HUMAN	POLR2E	physical	9201987
RPAB5_HUMAN	POLR2L	physical	9201987
RPAB3_HUMAN	POLR2H	physical	9201987
RPAP3_HUMAN	RPAP3	physical	17643375
RPB7_HUMAN	POLR2G	physical	17643375
RPAB3_HUMAN	POLR2H	physical	17643375
KDM1A_HUMAN	KDM1A	physical	17643375
LZTR1_HUMAN	LZTR1	physical	20211142
NEMO_HUMAN	IKBKG	physical	20211142
ZSCA1_HUMAN	ZSCAN1	physical	20211142
CTDP1_HUMAN	CTDP1	physical	15670829
RPB3_HUMAN	POLR2C	physical	22939629
RPB4_HUMAN	POLR2D	physical	22939629
RPB9_HUMAN	POLR2I	physical	22939629
TBA4A_HUMAN	TUBA4A	physical	22939629
TBB4B_HUMAN	TUBB4B	physical	22939629
VATB2_HUMAN	ATP6V1B2	physical	22939629
RPC6_HUMAN	POLR3F	physical	22939629
ESR1_HUMAN	ESR1	physical	16957778
SRC_HUMAN	SRC	physical	16957778
PSB9_HUMAN	PSMB9	physical	16957778
ELOA1_HUMAN	TCEB3	physical	16957778
EF1A1_HUMAN	EEF1A1	physical	26344197
SELB_HUMAN	EEFSEC	physical	26344197
T2FB_HUMAN	GTF2F2	physical	26344197
RPA1_HUMAN	POLR1A	physical	26344197
RPA2_HUMAN	POLR1B	physical	26344197
RPB1_HUMAN	POLR2A	physical	26344197
RPB3_HUMAN	POLR2C	physical	26344197
RPB4_HUMAN	POLR2D	physical	26344197
RPAB1_HUMAN	POLR2E	physical	26344197
RPAB2_HUMAN	POLR2F	physical	26344197
RPB9_HUMAN	POLR2I	physical	26344197
RPAB4_HUMAN	POLR2K	physical	26344197
RPC1_HUMAN	POLR3A	physical	26344197
RS5_HUMAN	RPS5	physical	26344197
TBA4A_HUMAN	TUBA4A	physical	26344197
TBB5_HUMAN	TUBB	physical	26344197
TBB4B_HUMAN	TUBB4B	physical	26344197

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.