RPB7_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RPB7_HUMAN
• UniProt AC: P62487
• Protein Name: DNA-directed RNA polymerase II subunit RPB7
• Gene Name: POLR2G
• Organism: Homo sapiens (Human).
• Sequence Length: 172
• Subcellular Localization: Nucleus .
• Protein Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA..
• Protein Sequence: MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MFYHISLEHE -----CCEEEEEEEE	9.28	24719451
6	Phosphorylation	--MFYHISLEHEILL --CCEEEEEEEEEEC	17.88	24719451
17	Phosphorylation	EILLHPRYFGPNLLN EEECCHHHCCHHHHH	19.83	28152594
27	Ubiquitination	PNLLNTVKQKLFTEV HHHHHHHHHHHCCEE	39.41	21906983
29	Ubiquitination	LLNTVKQKLFTEVEG HHHHHHHHHCCEECC	39.59	22817900
29	Acetylation	LLNTVKQKLFTEVEG HHHHHHHHHCCEECC	39.59	25953088
71	Ubiquitination	GFVLYPVKYKAIVFR CEEEEEECEEEEEEE	36.21	21906983
73	Ubiquitination	VLYPVKYKAIVFRPF EEEEECEEEEEEECC	26.39	22817900
81	Ubiquitination	AIVFRPFKGEVVDAV EEEEECCCCCEEEEE	57.77	23000965
81	Acetylation	AIVFRPFKGEVVDAV EEEEECCCCCEEEEE	57.77	26051181
129	Ubiquitination	NSNPPCYKTMDEDIV CCCCCCCCCCCCCEE	44.05	21963094
131	Sulfoxidation	NPPCYKTMDEDIVIQ CCCCCCCCCCCEEEE	4.67	21406390
146	Ubiquitination	QDDEIRLKIVGTRVD CCCEEEEEEEEEECC	26.04	21906983
146	2-Hydroxyisobutyrylation	QDDEIRLKIVGTRVD CCCEEEEEEEEEECC	26.04	-
154	Ubiquitination	IVGTRVDKNDIFAIG EEEEECCHHCEEEEH	54.37	22817900

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	VHL	P40337	PMID:12912922

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RPB7_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RPB7_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RPB3_HUMAN	POLR2C	physical	9201987
RPAB1_HUMAN	POLR2E	physical	9201987
RPAB2_HUMAN	POLR2F	physical	9201987
RPB7_HUMAN	POLR2G	physical	9201987
RPAB3_HUMAN	POLR2H	physical	9201987
MCAF1_MOUSE	Atf7ip	physical	10777215
PRKRA_HUMAN	PRKRA	physical	21988832
TNNC1_HUMAN	TNNC1	physical	21988832
ESR1_HUMAN	ESR1	physical	16957778
SRC_HUMAN	SRC	physical	16957778
PSB9_HUMAN	PSMB9	physical	16957778
ELOA1_HUMAN	TCEB3	physical	16957778
RPAP2_HUMAN	RPAP2	physical	26186194
RPB1_HUMAN	POLR2A	physical	26186194
RPB4_HUMAN	POLR2D	physical	26186194
RPR1A_HUMAN	RPRD1A	physical	26186194
SFR19_HUMAN	SCAF1	physical	26186194
RPRD2_HUMAN	RPRD2	physical	26186194
RECQ5_HUMAN	RECQL5	physical	26186194
RPB2_HUMAN	POLR2B	physical	26186194
GPN1_HUMAN	GPN1	physical	26186194
RPB11_HUMAN	POLR2J	physical	26186194
RPB9_HUMAN	POLR2I	physical	26186194
MED17_HUMAN	MED17	physical	26186194
RPAB5_HUMAN	POLR2L	physical	26186194
RPAB2_HUMAN	POLR2F	physical	26186194
MED26_HUMAN	MED26	physical	26186194
CTDP1_HUMAN	CTDP1	physical	26186194
MED30_HUMAN	MED30	physical	26186194
RPB1_HUMAN	POLR2A	physical	26344197
RPAB5_HUMAN	POLR2L	physical	26344197
IPP2_HUMAN	PPP1R2	physical	26344197
CX6B1_HUMAN	COX6B1	physical	26496610
TF2B_HUMAN	GTF2B	physical	26496610
T2FA_HUMAN	GTF2F1	physical	26496610
T2FB_HUMAN	GTF2F2	physical	26496610
NASP_HUMAN	NASP	physical	26496610
RPB1_HUMAN	POLR2A	physical	26496610
RPB2_HUMAN	POLR2B	physical	26496610
RPB3_HUMAN	POLR2C	physical	26496610
RPB4_HUMAN	POLR2D	physical	26496610
RPAB1_HUMAN	POLR2E	physical	26496610
RPAB3_HUMAN	POLR2H	physical	26496610
RPB9_HUMAN	POLR2I	physical	26496610
RPB11_HUMAN	POLR2J	physical	26496610
MED1_HUMAN	MED1	physical	26496610
SPT5H_HUMAN	SUPT5H	physical	26496610
TCEA1_HUMAN	TCEA1	physical	26496610
MOGS_HUMAN	MOGS	physical	26496610
CTDP1_HUMAN	CTDP1	physical	26496610
MED14_HUMAN	MED14	physical	26496610
RECQ5_HUMAN	RECQL5	physical	26496610
MED21_HUMAN	MED21	physical	26496610
MED17_HUMAN	MED17	physical	26496610
MED26_HUMAN	MED26	physical	26496610
MED6_HUMAN	MED6	physical	26496610
MED16_HUMAN	MED16	physical	26496610
PUR6_HUMAN	PAICS	physical	26496610
GPN1_HUMAN	GPN1	physical	26496610
SETX_HUMAN	SETX	physical	26496610
RPRD2_HUMAN	RPRD2	physical	26496610
MED4_HUMAN	MED4	physical	26496610
MED31_HUMAN	MED31	physical	26496610
GPN3_HUMAN	GPN3	physical	26496610
MED15_HUMAN	MED15	physical	26496610
PAF1_HUMAN	PAF1	physical	26496610
MED18_HUMAN	MED18	physical	26496610
MCM10_HUMAN	MCM10	physical	26496610
MED29_HUMAN	MED29	physical	26496610
CDC73_HUMAN	CDC73	physical	26496610
RPAP2_HUMAN	RPAP2	physical	26496610
MYZAP_HUMAN	POLR2M	physical	26496610
GRL1A_HUMAN	POLR2M	physical	26496610
GL1AD_HUMAN	POLR2M	physical	26496610
RECQ5_HUMAN	RECQL5	physical	28514442
RPAP2_HUMAN	RPAP2	physical	28514442
RPR1A_HUMAN	RPRD1A	physical	28514442
CTDP1_HUMAN	CTDP1	physical	28514442
RPRD2_HUMAN	RPRD2	physical	28514442
RPB9_HUMAN	POLR2I	physical	28514442
RPB1_HUMAN	POLR2A	physical	28514442
RPAB5_HUMAN	POLR2L	physical	28514442
RPB11_HUMAN	POLR2J	physical	28514442
MED26_HUMAN	MED26	physical	28514442
RPB2_HUMAN	POLR2B	physical	28514442
GPN1_HUMAN	GPN1	physical	28514442
MED30_HUMAN	MED30	physical	28514442
RPB3_HUMAN	POLR2C	physical	28514442
RPAB3_HUMAN	POLR2H	physical	28514442
SFR19_HUMAN	SCAF1	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.