MED4_HUMAN - dbPTM
MED4_HUMAN - PTM Information in dbPTM
Basic Information of Protein
UniProt ID MED4_HUMAN
UniProt AC Q9NPJ6
Protein Name Mediator of RNA polymerase II transcription subunit 4
Gene Name MED4
Organism Homo sapiens (Human).
Sequence Length 270
Subcellular Localization Nucleus.
Protein Description Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors..
Protein Sequence MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDSDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKENEDDVEIMSTDSSSSSSESD
Overview of Protein Modification Sites with Functional and Structural Information
Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations Modification Substrate Peptides
&
Secondary Structure
ASA (%) Reference Orthologous
Protein Cluster
2Acetylation------MAASSSGEK
------CCCCCCCHH
18.9522814378
4Phosphorylation----MAASSSGEKEK
----CCCCCCCHHHH
18.5929083192
5Phosphorylation---MAASSSGEKEKE
---CCCCCCCHHHHH
37.3229083192
6Phosphorylation--MAASSSGEKEKER
--CCCCCCCHHHHHH
48.7729083192
9UbiquitinationAASSSGEKEKERLGG
CCCCCCHHHHHHHCC
77.0721890473
32PhosphorylationSTRERLLSALEDLEV
HHHHHHHHHHHHHHH
35.0922617229
35UbiquitinationERLLSALEDLEVLSR
HHHHHHHHHHHHHHH
61.3321890473
51PhosphorylationLIEMLAISRNQKLLQ
HHHHHHHHHCHHHHH
21.4124719451
55AcetylationLAISRNQKLLQAGEE
HHHHHCHHHHHCCCH
55.5025953088
55UbiquitinationLAISRNQKLLQAGEE
HHHHHCHHHHHCCCH
55.5021890473
66UbiquitinationAGEENQVLELLIHRD
CCCHHHHHHHHHCCC
2.5021890473
81UbiquitinationGEFQELMKLALNQGK
CHHHHHHHHHHHCCC
43.1421890473
88UbiquitinationKLALNQGKIHHEMQV
HHHHHCCCCHHHHHH
29.15-
98UbiquitinationHEMQVLEKEVEKRDS
HHHHHHHHHHHHCHH
64.00-
104UbiquitinationEKEVEKRDSDIQQLQ
HHHHHHCHHHHHHHH
62.8321890473
105PhosphorylationKEVEKRDSDIQQLQK
HHHHHCHHHHHHHHH
40.4225056879
112UbiquitinationSDIQQLQKQLKEAEQ
HHHHHHHHHHHHHHH
67.2121890473
115UbiquitinationQQLQKQLKEAEQILA
HHHHHHHHHHHHHHH
53.00-
123PhosphorylationEAEQILATAVYQAKE
HHHHHHHHHHHHHHH
17.1429978859
126PhosphorylationQILATAVYQAKEKLK
HHHHHHHHHHHHHHH
10.5729978859
129UbiquitinationATAVYQAKEKLKSIE
HHHHHHHHHHHHHHH
39.60-
131UbiquitinationAVYQAKEKLKSIEKA
HHHHHHHHHHHHHHH
61.58-
134PhosphorylationQAKEKLKSIEKARKG
HHHHHHHHHHHHHCC
46.5524719451
140UbiquitinationKSIEKARKGAISSEE
HHHHHHHCCCCCHHH
58.51-
150UbiquitinationISSEEIIKYAHRISA
CCHHHHHHHHHHHCC
41.8821890473
150AcetylationISSEEIIKYAHRISA
CCHHHHHHHHHHHCC
41.8826051181

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)
Modified Location Modified Residue Modification Type of Upstream Proteins Gene Name of Upstream Proteins UniProt AC of Upstream Proteins Sources

Oops, there are no upstream regulatory protein records of MED4_HUMAN !!

Functions of PTM Sites
Modified Location Modified Residue Modification Function Reference

Oops, there are no descriptions of PTM sites of MED4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location Modification Variant Position
(Distance <= 10)
Residue Change SAP Related Disease Reference

Oops, there are no SNP-PTM records of MED4_HUMAN !!

Protein-Protein Interaction
Interacting Protein Gene Name Interaction Type PPI Reference Domain-Domain Interactions
PHS2_HUMANPCBD2physical
20211142
MED18_HUMANMED18physical
18250152
PUM2_HUMANPUM2physical
12421765
MED13_HUMANMED13physical
12421765
WDTC1_HUMANWDTC1physical
12421765
FOXJ3_HUMANFOXJ3physical
12421765
MED9_HUMANMED9physical
22939629
MED6_HUMANMED6physical
22939629
MED8_HUMANMED8physical
22939629
MK67I_HUMANNIFKphysical
22939629
CCHCR_HUMANCCHCR1physical
25416956
CTBL1_HUMANCTNNBL1physical
25416956
CC146_HUMANCCDC146physical
25416956
USBP1_HUMANUSHBP1physical
25416956
IFT20_HUMANIFT20physical
25416956
SGF29_HUMANCCDC101physical
25416956
HAUS1_HUMANHAUS1physical
25416956
FRMD6_HUMANFRMD6physical
25416956
MB213_HUMANMAB21L3physical
25416956
CA216_HUMANC1orf216physical
25416956
AMOT_HUMANAMOTphysical
25416956
TXLNA_HUMANTXLNAphysical
25416956
FA13C_HUMANFAM13Cphysical
25416956
MED10_HUMANMED10physical
26344197
MED11_HUMANMED11physical
26344197
MED12_HUMANMED12physical
26344197
MED16_HUMANMED16physical
26344197
MED17_HUMANMED17physical
26344197
MED19_HUMANMED19physical
26344197
MED22_HUMANMED22physical
26344197
MED24_HUMANMED24physical
26344197
MED27_HUMANMED27physical
26344197
PCM1_HUMANPCM1physical
25281560
CP131_HUMANCEP131physical
25281560
OFD1_HUMANOFD1physical
25281560
CC138_HUMANCCDC138physical
25281560
MED1_HUMANMED1physical
25281560
HAUS6_HUMANHAUS6physical
25281560
PCNT_HUMANPCNTphysical
25281560
AHNK_HUMANAHNAKphysical
25281560
PIBF1_HUMANPIBF1physical
25281560
MED14_HUMANMED14physical
25281560
CCD14_HUMANCCDC14physical
25281560
MOONR_HUMANKIAA0753physical
25281560
MED12_HUMANMED12physical
25281560
HAUS5_HUMANHAUS5physical
25281560
HAUS3_HUMANHAUS3physical
25281560
CD2AP_HUMANCD2APphysical
25281560
CE170_HUMANCEP170physical
25281560
MIB1_HUMANMIB1physical
25281560
GIT1_HUMANGIT1physical
25281560
CCD18_HUMANCCDC18physical
25281560
KIF7_HUMANKIF7physical
25281560
HAUS4_HUMANHAUS4physical
25281560
CE350_HUMANCEP350physical
25281560
NBN_HUMANNBNphysical
25281560
FLNB_HUMANFLNBphysical
25281560
MED13_HUMANMED13physical
25281560
CE290_HUMANCEP290physical
25281560
KIF3A_HUMANKIF3Aphysical
25281560
MD13L_HUMANMED13Lphysical
25281560
HAUS1_HUMANHAUS1physical
25281560
SQSTM_HUMANSQSTM1physical
25281560
KINH_HUMANKIF5Bphysical
25281560
CKAP5_HUMANCKAP5physical
25281560
MED17_HUMANMED17physical
25281560
TBC31_HUMANTBC1D31physical
25281560
CEP72_HUMANCEP72physical
25281560
DVL2_HUMANDVL2physical
25281560
ADIP_HUMANSSX2IPphysical
25281560
MED8_HUMANMED8physical
25281560
HAUS8_HUMANHAUS8physical
25281560
MYCB2_HUMANMYCBP2physical
25281560
MED27_HUMANMED27physical
25281560
CEP55_HUMANCEP55physical
25281560
RPB1_HUMANPOLR2Aphysical
25281560
XRN1_HUMANXRN1physical
25281560
RB6I2_HUMANERC1physical
25281560
EXOC4_HUMANEXOC4physical
25281560
LUZP1_HUMANLUZP1physical
25281560
ARHG7_HUMANARHGEF7physical
25281560
ANR26_HUMANANKRD26physical
25281560
DCTN2_HUMANDCTN2physical
25281560
KIF11_HUMANKIF11physical
25281560
CSPP1_HUMANCSPP1physical
25281560
GIT2_HUMANGIT2physical
25281560
ACTZ_HUMANACTR1Aphysical
25281560
E41L3_HUMANEPB41L3physical
25281560
SRP68_HUMANSRP68physical
25281560
CCD61_HUMANCCDC61physical
25281560
CE162_HUMANCEP162physical
25281560
NSF1C_HUMANNSFL1Cphysical
25281560
PUR8_HUMANADSLphysical
25281560
TLN1_HUMANTLN1physical
25281560
MED20_HUMANMED20physical
25281560
EDRF1_HUMANEDRF1physical
25281560
SLK_HUMANSLKphysical
25281560
SPAG5_HUMANSPAG5physical
25281560
MAP1B_HUMANMAP1Bphysical
25281560
MED6_HUMANMED6physical
25281560
HAUS2_HUMANHAUS2physical
25281560
MPP9_HUMANMPHOSPH9physical
25281560
CCD77_HUMANCCDC77physical
25281560
NIN_HUMANNINphysical
25281560
CE152_HUMANCEP152physical
25281560
ODB2_HUMANDBTphysical
25281560
MED22_HUMANMED22physical
25281560
DLG5_HUMANDLG5physical
25281560
RAI14_HUMANRAI14physical
25281560
MED21_HUMANMED21physical
25281560
KIF5A_HUMANKIF5Aphysical
25281560
TBK1_HUMANTBK1physical
25281560
PSMD7_HUMANPSMD7physical
25281560
ODFP2_HUMANODF2physical
25281560
PUR6_HUMANPAICSphysical
25281560
IFT74_HUMANIFT74physical
25281560
ASPP2_HUMANTP53BP2physical
25281560
MED18_HUMANMED18physical
25281560
MED9_HUMANMED9physical
25281560
MED26_HUMANMED26physical
25281560
ECD_HUMANECDphysical
25281560
HAUS7_HUMANHAUS7physical
25281560
MED19_HUMANMED19physical
25281560
TXLNG_HUMANTXLNGphysical
25281560
MA7D3_HUMANMAP7D3physical
25281560
MED11_HUMANMED11physical
25281560
CP110_HUMANCCP110physical
25281560
MED30_HUMANMED30physical
25281560
MED31_HUMANMED31physical
25281560
SPICE_HUMANSPICE1physical
25281560
TTLL5_HUMANTTLL5physical
25281560
MED10_HUMANMED10physical
25281560
IFIT5_HUMANIFIT5physical
25281560
ATG2B_HUMANATG2Bphysical
25281560
EP15R_HUMANEPS15L1physical
25281560
JIP4_HUMANSPAG9physical
25281560
CCD66_HUMANCCDC66physical
25281560
EPN4_HUMANCLINT1physical
25281560
KEAP1_HUMANKEAP1physical
25281560
MICA3_HUMANMICAL3physical
25281560
ODF2L_HUMANODF2Lphysical
25281560
UBE2O_HUMANUBE2Ophysical
25281560
CAMP1_HUMANCAMSAP1physical
25281560
EDC4_HUMANEDC4physical
25281560
IFT81_HUMANIFT81physical
25281560
SDCG3_HUMANSDCCAG3physical
25281560
PSA2_HUMANPSMA2physical
25281560
TRI25_HUMANTRIM25physical
25281560
ALMS1_HUMANALMS1physical
25281560
SKA3_HUMANSKA3physical
25281560
MED24_HUMANMED24physical
25281560
TXLNA_HUMANTXLNAphysical
25281560
MED28_HUMANMED28physical
25281560
CCD22_HUMANCCDC22physical
25281560
WRP73_HUMANWRAP73physical
25281560
SPC24_HUMANSPC24physical
25281560
BUB1B_HUMANBUB1Bphysical
25281560
CNO10_HUMANCNOT10physical
25281560
LRBA_HUMANLRBAphysical
25281560
PDLI5_HUMANPDLIM5physical
25281560
UTRO_HUMANUTRNphysical
25281560
WDR90_HUMANWDR90physical
25281560
CNOT1_HUMANCNOT1physical
25281560
CNTRB_HUMANCNTROBphysical
25281560
SHOT1_HUMANKIAA1598physical
25281560
CNO11_HUMANCNOT11physical
25281560
EIF3M_HUMANEIF3Mphysical
25281560
41_HUMANEPB41physical
25281560
WNK1_HUMANWNK1physical
25281560
TTC28_HUMANTTC28physical
25281560
C2CD3_HUMANC2CD3physical
25281560
CYLD_HUMANCYLDphysical
25281560
DCTN3_HUMANDCTN3physical
25281560
DPYL3_HUMANDPYSL3physical
25281560
ERBIN_HUMANERBB2IPphysical
25281560
RFLB_HUMANFAM101Bphysical
25281560
KANK2_HUMANKANK2physical
25281560
CIP2A_HUMANKIAA1524physical
25281560
LSM12_HUMANLSM12physical
25281560
NDC80_HUMANNDC80physical
25281560
PSB1_HUMANPSMB1physical
25281560
RBM11_HUMANRBM11physical
25281560
SHLB2_HUMANSH3GLB2physical
25281560
SNX1_HUMANSNX1physical
25281560
UBP15_HUMANUSP15physical
25281560
ZFY16_HUMANZFYVE16physical
25281560
ZYX_HUMANZYXphysical
25281560
PDIA6_HUMANPDIA6physical
25281560
CP135_HUMANCEP135physical
25281560
CPSF3_HUMANCPSF3physical
25281560
F111B_HUMANFAM111Bphysical
25281560
F91A1_HUMANFAM91A1physical
25281560
KIF14_HUMANKIF14physical
25281560
SCRIB_HUMANSCRIBphysical
25281560
SNP29_HUMANSNAP29physical
25281560
DEN4C_HUMANDENND4Cphysical
25281560
DYST_HUMANDSTphysical
25281560
FR1OP_HUMANFGFR1OPphysical
25281560
NAV1_HUMANNAV1physical
25281560
NEK9_HUMANNEK9physical
25281560
RPAP2_HUMANRPAP2physical
25281560
TCHP_HUMANTCHPphysical
25281560
AT5F1_HUMANATP5F1physical
25281560
PDCD6_HUMANPDCD6physical
25281560
DCAM_HUMANAMD1physical
25281560
DHX40_HUMANDHX40physical
25281560
LARP7_HUMANLARP7physical
25281560
MOGS_HUMANMOGSphysical
25281560
NUFP2_HUMANNUFIP2physical
25281560
SCO1_HUMANSCO1physical
25281560
ZC3HF_HUMANZC3H15physical
25281560
DNJB1_HUMANDNAJB1physical
25281560
RRP1_HUMANRRP1physical
25281560
SRSF5_HUMANSRSF5physical
25281560
TNR6B_HUMANTNRC6Bphysical
25281560
ATP5H_HUMANATP5Hphysical
25281560
SDE2_HUMANSDE2physical
25281560
MCA3_HUMANEEF1E1physical
25281560
FIP1_HUMANFIP1L1physical
25281560
GLYR1_HUMANGLYR1physical
25281560
GNAO_HUMANGNAO1physical
25281560
HBB_HUMANHBBphysical
25281560
MESD_HUMANMESDC2physical
25281560
RT17_HUMANMRPS17physical
25281560
NOP2_HUMANNOP2physical
25281560
PI42A_HUMANPIP4K2Aphysical
25281560
RAB5A_HUMANRAB5Aphysical
25281560
RANB9_HUMANRANBP9physical
25281560
S61A1_HUMANSEC61A1physical
25281560
TAXB1_HUMANTAX1BP1physical
25281560
TXND9_HUMANTXNDC9physical
25281560
WDR33_HUMANWDR33physical
25281560
BAG6_HUMANBAG6physical
25281560
CSK22_HUMANCSNK2A2physical
25281560
ATIF1_HUMANATPIF1physical
25281560
LRC47_HUMANLRRC47physical
25281560
CRIPT_HUMANCRIPTphysical
25281560
AP2A1_HUMANAP2A1physical
25281560
VATB2_HUMANATP6V1B2physical
25281560
RRFM_HUMANMRRFphysical
25281560
ZMAT2_HUMANZMAT2physical
25281560
MED25_HUMANMED25physical
25281560
CND3_HUMANNCAPGphysical
25281560
T2FA_HUMANGTF2F1physical
25281560
FA50A_HUMANFAM50Aphysical
25281560
CAP2_HUMANCAP2physical
25281560
SRRM1_HUMANSRRM1physical
25281560
AMOT_HUMANAMOTphysical
25281560
ARRB2_HUMANARRB2physical
25281560
BRX1_HUMANBRIX1physical
25281560
EXOC1_HUMANEXOC1physical
25281560
NOP56_HUMANNOP56physical
25281560
APR_HUMANPMAIP1physical
25281560
CYC_HUMANCYCSphysical
25281560
MANF_HUMANMANFphysical
25281560
EXOS8_HUMANEXOSC8physical
25281560
SPTN1_HUMANSPTAN1physical
25281560
CD11A_HUMANCDK11Aphysical
25281560
FA5_HUMANF5physical
25281560
RBM27_HUMANRBM27physical
25281560
SP16H_HUMANSUPT16Hphysical
25281560
XRCC4_HUMANXRCC4physical
25281560
CERU_HUMANCPphysical
25281560
ECHB_HUMANHADHBphysical
25281560
A2MG_HUMANA2Mphysical
25281560
SERF1_HUMANSERF1Aphysical
25281560
ZN638_HUMANZNF638physical
25281560
COR1C_HUMANCORO1Cphysical
25281560
THOC2_HUMANTHOC2physical
25281560
DHE4_HUMANGLUD2physical
25281560
RL1D1_HUMANRSL1D1physical
25281560
SSNA1_HUMANSSNA1physical
25281560
SYK_HUMANKARSphysical
25281560
ARHG6_HUMANARHGEF6physical
25281560
MOB2_HUMANMOB2physical
25281560
PI51A_HUMANPIP5K1Aphysical
25281560
FLII_HUMANFLIIphysical
25281560
SKA1_HUMANSKA1physical
25281560
CDK8_HUMANCDK8physical
25281560
TCEA1_HUMANTCEA1physical
25281560
RUXG_HUMANSNRPGphysical
25281560
ALBU_HUMANALBphysical
25281560
HEMO_HUMANHPXphysical
25281560
CCNC_HUMANCCNCphysical
25281560
DZIP3_HUMANDZIP3physical
25281560
ERCC3_HUMANERCC3physical
25281560
RBM14_HUMANRBM14physical
25281560
HOME1_HUMANHOMER1physical
25281560
RNPS1_HUMANRNPS1physical
25281560
RSRC2_HUMANRSRC2physical
25281560
ATPG_HUMANATP5C1physical
25281560
CPSF7_HUMANCPSF7physical
25281560
HPBP1_HUMANHSPBP1physical
25281560
PI42B_HUMANPIP4K2Bphysical
25281560
RPB2_HUMANPOLR2Bphysical
25281560
CO3_HUMANC3physical
25281560
PI42C_HUMANPIP4K2Cphysical
25281560
QPCT_HUMANQPCTphysical
25281560
MOFA1_HUMANMRFAP1physical
25281560
MED29_HUMANMED29physical
25281560
M3K7_HUMANMAP3K7physical
25281560
MED7_HUMANMED7physical
25281560
TF2B_HUMANGTF2Bphysical
25281560
MED16_HUMANMED16physical
25281560
ECHA_HUMANHADHAphysical
25281560
PSME3_HUMANPSME3physical
25281560
HAP28_HUMANPDAP1physical
25281560
MED15_HUMANMED15physical
25281560
UBP2L_HUMANUBAP2Lphysical
25281560
IFT20_HUMANIFT20physical
25281560
TR150_HUMANTHRAP3physical
25281560
BCLF1_HUMANBCLAF1physical
25281560
ACAP2_HUMANACAP2physical
25281560
MED23_HUMANMED23physical
25281560
RAE1L_HUMANRAE1physical
25281560
RIOK1_HUMANRIOK1physical
25281560
CCAR1_HUMANCCAR1physical
25281560
TRIPB_HUMANTRIP11physical
27173435

Drug and Disease Associations
Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.
Regulatory Network of MED4_HUMAN

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Related Literatures of Post-Translational Modification
Phosphorylation
ReferencePubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND MASSSPECTROMETRY.
"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32, AND MASSSPECTROMETRY.

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