dbPTM

CDK8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	CDK8_HUMAN
UniProt AC	P49336
Protein Name	Cyclin-dependent kinase 8
Gene Name	CDK8
Organism	Homo sapiens (Human).
Sequence Length	464
Subcellular Localization	Nucleus .
Protein Description	Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation..
Protein Sequence	MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLEDPLPTSDVFAGCQIPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	DFKVKLSSERERVED CCEEECCCHHHHHHH	51.62	-
26	Ubiquitination	LFEYEGCKVGRGTYG HHHCCCCEECCCCCC	59.59	-
31	Phosphorylation	GCKVGRGTYGHVYKA CCEECCCCCCEEEEC	25.60	-
52	Ubiquitination	DDKDYALKQIEGTGI CCHHHHHHHHCCCCC	40.85	-
62	Phosphorylation	EGTGISMSACREIAL CCCCCCHHHHHHHHH	19.89	24043423
74	Ubiquitination	IALLRELKHPNVISL HHHHHHCCCCCEEEE	52.87	29967540
80	Phosphorylation	LKHPNVISLQKVFLS CCCCCEEEEEEHHHH	22.06	24719451
119	Ubiquitination	RASKANKKPVQLPRG HCHHCCCCCCCCCHH	50.67	29967540
130	Phosphorylation	LPRGMVKSLLYQILD CCHHHHHHHHHHHHH	16.52	-
133	Phosphorylation	GMVKSLLYQILDGIH HHHHHHHHHHHHHHH	9.98	-
141	Phosphorylation	QILDGIHYLHANWVL HHHHHHHHHCCCEEE	9.77	-
272	Ubiquitination	KDWEDIKKMPEHSTL CCHHHHHHCCCCCHH	61.08	29967540
292	Phosphorylation	RNTYTNCSLIKYMEK HHCCCCHHHHHHHHH	34.89	24719451
295	Acetylation	YTNCSLIKYMEKHKV CCCHHHHHHHHHCCC	43.78	26051181
322	Ubiquitination	LLTMDPIKRITSEQA HHCCCHHHHCCCHHH	43.04	29967540
360	Phosphorylation	YPKREFLTEEEPDDK CCCCCCCCCCCCCCC	46.82	-
367	Acetylation	TEEEPDDKGDKKNQQ CCCCCCCCCCHHHHH	76.12	23236377
402	Acetylation	HTQGPPLKKVRVVPP CCCCCCCCEEEEECC	55.80	24180087
410	Phosphorylation	KVRVVPPTTTSGGLI EEEEECCCCCCCCEE	36.31	19369195
411	Phosphorylation	VRVVPPTTTSGGLIM EEEECCCCCCCCEEE	25.41	24275569
411	O-linked_Glycosylation	VRVVPPTTTSGGLIM EEEECCCCCCCCEEE	25.41	30059200
412	O-linked_Glycosylation	RVVPPTTTSGGLIMT EEECCCCCCCCEEEC	28.05	30059200
413	Phosphorylation	VVPPTTTSGGLIMTS EECCCCCCCCEEECC	28.86	22817900
413	O-linked_Glycosylation	VVPPTTTSGGLIMTS EECCCCCCCCEEECC	28.86	30059200
419	Phosphorylation	TSGGLIMTSDYQRSN CCCCEEECCCCCCCC	16.08	24275569

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	SKP2	Q13309	PMID:22898779

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of CDK8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of CDK8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CEBPB_HUMAN	CEBPB	physical	14759369
CCNC_HUMAN	CCNC	physical	9150135
CBP_HUMAN	CREBBP	physical	9710619
RPB1_HUMAN	POLR2A	physical	9710619
KAT2B_HUMAN	KAT2B	physical	9710619
SNF5_HUMAN	SMARCB1	physical	9710619
SMRC2_HUMAN	SMARCC2	physical	9710619
SMRC1_HUMAN	SMARCC1	physical	9710619
CCNC_HUMAN	CCNC	physical	9710619
MED21_HUMAN	MED21	physical	9710619
SMCA2_HUMAN	SMARCA2	physical	9710619
CCNC_HUMAN	CCNC	physical	11416138
MED23_HUMAN	MED23	physical	11416138
MED14_HUMAN	MED14	physical	11416138
MED6_HUMAN	MED6	physical	11416138
MED13_HUMAN	MED13	physical	10198638
MED12_HUMAN	MED12	physical	10198638
MED1_HUMAN	MED1	physical	10198638
MED14_HUMAN	MED14	physical	10198638
TR150_HUMAN	THRAP3	physical	10198638
MED24_HUMAN	MED24	physical	10198638
MED16_HUMAN	MED16	physical	10198638
MED17_HUMAN	MED17	physical	10198638
MED6_HUMAN	MED6	physical	10198638
MED31_HUMAN	MED31	physical	10198638
MED21_HUMAN	MED21	physical	10198638
MED10_HUMAN	MED10	physical	10198638
MED13_HUMAN	MED13	physical	11867769
MED12_HUMAN	MED12	physical	11867769
MED1_HUMAN	MED1	physical	11867769
MED23_HUMAN	MED23	physical	11867769
MED24_HUMAN	MED24	physical	11867769
MED16_HUMAN	MED16	physical	11867769
MED17_HUMAN	MED17	physical	11867769
MED7_HUMAN	MED7	physical	11867769
CCNC_HUMAN	CCNC	physical	11867769
MED6_HUMAN	MED6	physical	11867769
MED20_HUMAN	MED20	physical	11867769
MED21_HUMAN	MED21	physical	11867769
MED10_HUMAN	MED10	physical	11867769
CCNC_HUMAN	CCNC	physical	7568034
PARP1_HUMAN	PARP1	physical	16204234
MED6_HUMAN	MED6	physical	15808511
PARP1_HUMAN	PARP1	physical	15808511
MED1_HUMAN	MED1	physical	20098423
MED12_HUMAN	MED12	physical	20098423
MED13_HUMAN	MED13	physical	20098423
MED14_HUMAN	MED14	physical	20098423
MED23_HUMAN	MED23	physical	20098423
MED22_HUMAN	MED22	physical	20098423
MED15_HUMAN	MED15	physical	20098423
MED16_HUMAN	MED16	physical	20098423
MED9_HUMAN	MED9	physical	20098423
MED17_HUMAN	MED17	physical	20098423
MED26_HUMAN	MED26	physical	20098423
MED1_HUMAN	MED1	physical	10024883
MED14_HUMAN	MED14	physical	10024883
MED24_HUMAN	MED24	physical	10024883
MED6_HUMAN	MED6	physical	10024883
CCNC_HUMAN	CCNC	physical	10024883
MED21_HUMAN	MED21	physical	10024883
MED31_HUMAN	MED31	physical	10024883
MED10_HUMAN	MED10	physical	10024883
MED13_HUMAN	MED13	physical	10882111
MED12_HUMAN	MED12	physical	10882111
MED1_HUMAN	MED1	physical	10882111
MED14_HUMAN	MED14	physical	10882111
MED24_HUMAN	MED24	physical	10882111
MED16_HUMAN	MED16	physical	10882111
MED17_HUMAN	MED17	physical	10882111
MED7_HUMAN	MED7	physical	10882111
MED20_HUMAN	MED20	physical	10882111
CCNC_HUMAN	CCNC	physical	10882111
MED21_HUMAN	MED21	physical	10882111
MED6_HUMAN	MED6	physical	10882111
SMAD2_HUMAN	SMAD2	physical	19914168
SMAD3_HUMAN	SMAD3	physical	19914168
SMAD1_HUMAN	SMAD1	physical	19914168
CTDP1_HUMAN	CTDP1	physical	19914168
SMAD5_HUMAN	SMAD5	physical	19914168
MED23_HUMAN	MED23	physical	10353252
MED7_HUMAN	MED7	physical	10353252
CCNC_HUMAN	CCNC	physical	10353252
SMC1A_HUMAN	SMC1A	physical	20720539
SMC3_HUMAN	SMC3	physical	20720539
MED12_HUMAN	MED12	physical	20720539
MED15_HUMAN	MED15	physical	20720539
NIPBL_HUMAN	NIPBL	physical	20720539
SMCA4_HUMAN	SMARCA4	physical	22668559
BCL6_HUMAN	BCL6	physical	22668559
SUZ12_HUMAN	SUZ12	physical	22668559
CDK8_HUMAN	CDK8	physical	22379099
H32_HUMAN	HIST2H3C	physical	22379099
NRIP1_HUMAN	NRIP1	physical	21868449
RPB1_HUMAN	POLR2A	physical	15009212
RPB1_HUMAN	POLR2A	physical	11278802
MED12_HUMAN	MED12	physical	23322298
MED13_HUMAN	MED13	physical	23322298
MED23_HUMAN	MED23	physical	23322298
MED1_HUMAN	MED1	physical	23322298
CDK8_HUMAN	CDK8	physical	23322298
MED24_HUMAN	MED24	physical	23322298
CCNC_HUMAN	CCNC	physical	15328539
MBP_HUMAN	MBP	physical	15328539
ERCC3_HUMAN	ERCC3	physical	10993082
CCNH_HUMAN	CCNH	physical	10993082
HERC2_HUMAN	HERC2	physical	23602568
MED12_HUMAN	MED12	physical	23602568
MED1_HUMAN	MED1	physical	23602568
MED13_HUMAN	MED13	physical	23602568
MED14_HUMAN	MED14	physical	23602568
MED16_HUMAN	MED16	physical	23602568
MED17_HUMAN	MED17	physical	23602568
MD13L_HUMAN	MED13L	physical	23602568
MED4_HUMAN	MED4	physical	23602568
MED15_HUMAN	MED15	physical	23602568
MED6_HUMAN	MED6	physical	23602568
MED24_HUMAN	MED24	physical	23602568
MED8_HUMAN	MED8	physical	23602568
CCNC_HUMAN	CCNC	physical	23602568
MED23_HUMAN	MED23	physical	23602568
MED27_HUMAN	MED27	physical	23602568
MED20_HUMAN	MED20	physical	23602568
MED29_HUMAN	MED29	physical	23602568
MED31_HUMAN	MED31	physical	23602568
MED28_HUMAN	MED28	physical	23602568
MED22_HUMAN	MED22	physical	23602568
MED7_HUMAN	MED7	physical	23602568
MED18_HUMAN	MED18	physical	23602568
MED9_HUMAN	MED9	physical	23602568
MED11_HUMAN	MED11	physical	23602568
CDK8_HUMAN	CDK8	physical	23602568
MED10_HUMAN	MED10	physical	23602568
HNRPF_HUMAN	HNRNPF	physical	23602568
MED30_HUMAN	MED30	physical	23602568
MED21_HUMAN	MED21	physical	23602568
MED6_HUMAN	MED6	physical	26186194
MED4_HUMAN	MED4	physical	26186194
MED28_HUMAN	MED28	physical	26186194
MED17_HUMAN	MED17	physical	26186194
MED23_HUMAN	MED23	physical	26186194
MED29_HUMAN	MED29	physical	26186194
MED22_HUMAN	MED22	physical	26186194
MED24_HUMAN	MED24	physical	26186194
MED10_HUMAN	MED10	physical	26186194
CDK16_HUMAN	CDK16	physical	26186194
MED15_HUMAN	MED15	physical	26186194
MED9_HUMAN	MED9	physical	26186194
MED20_HUMAN	MED20	physical	26186194
MED8_HUMAN	MED8	physical	26186194
MED25_HUMAN	MED25	physical	26186194
MED30_HUMAN	MED30	physical	26186194
MYPT1_HUMAN	PPP1R12A	physical	26186194
MED12_HUMAN	MED12	physical	26186194
MD12L_HUMAN	MED12L	physical	26186194
MED13_HUMAN	MED13	physical	26186194
CDK19_HUMAN	CDK19	physical	26186194
MED27_HUMAN	MED27	physical	26186194
MD13L_HUMAN	MED13L	physical	26186194
MED1_HUMAN	MED1	physical	26186194
MED16_HUMAN	MED16	physical	26186194
MED14_HUMAN	MED14	physical	26186194
MED24_HUMAN	MED24	physical	26344197
MED6_HUMAN	MED6	physical	26344197
MED8_HUMAN	MED8	physical	26344197
RPB1_HUMAN	POLR2A	physical	15328539
CDK8_HUMAN	CDK8	physical	15328539
CCNC_HUMAN	CCNC	physical	26496610
RPB3_HUMAN	POLR2C	physical	26496610
MED1_HUMAN	MED1	physical	26496610
RBM4_HUMAN	RBM4	physical	26496610
MED22_HUMAN	MED22	physical	26496610
TAF1_HUMAN	TAF1	physical	26496610
TBX3_HUMAN	TBX3	physical	26496610
GCFC2_HUMAN	GCFC2	physical	26496610
ZN131_HUMAN	ZNF131	physical	26496610
BAG6_HUMAN	BAG6	physical	26496610
UBL4A_HUMAN	UBL4A	physical	26496610
CTDP1_HUMAN	CTDP1	physical	26496610
MED14_HUMAN	MED14	physical	26496610
MED21_HUMAN	MED21	physical	26496610
MED23_HUMAN	MED23	physical	26496610
MED17_HUMAN	MED17	physical	26496610
MED27_HUMAN	MED27	physical	26496610
MED20_HUMAN	MED20	physical	26496610
MED24_HUMAN	MED24	physical	26496610
ST65G_HUMAN	SUPT7L	physical	26496610
MED12_HUMAN	MED12	physical	26496610
MED13_HUMAN	MED13	physical	26496610
MED6_HUMAN	MED6	physical	26496610
MED16_HUMAN	MED16	physical	26496610
CELF1_HUMAN	CELF1	physical	26496610
WWP1_HUMAN	WWP1	physical	26496610
MD13L_HUMAN	MED13L	physical	26496610
GEMI5_HUMAN	GEMIN5	physical	26496610
MTBP_HUMAN	MTBP	physical	26496610
MED4_HUMAN	MED4	physical	26496610
MED31_HUMAN	MED31	physical	26496610
PCF11_HUMAN	PCF11	physical	26496610
MED15_HUMAN	MED15	physical	26496610
GET4_HUMAN	GET4	physical	26496610
MED18_HUMAN	MED18	physical	26496610
MED9_HUMAN	MED9	physical	26496610
MED29_HUMAN	MED29	physical	26496610
DEN4C_HUMAN	DENND4C	physical	26496610
SRGP1_HUMAN	SRGAP1	physical	26496610
MED28_HUMAN	MED28	physical	26496610
MED25_HUMAN	MED25	physical	26496610
MED10_HUMAN	MED10	physical	26496610
BUD13_HUMAN	BUD13	physical	26496610
MED30_HUMAN	MED30	physical	26496610
MED8_HUMAN	MED8	physical	26496610
RBM45_HUMAN	RBM45	physical	26496610
MED11_HUMAN	MED11	physical	26496610
H3C_HUMAN	H3F3C	physical	26496610
RPB1_HUMAN	POLR2A	physical	22379099
ANM5_HUMAN	PRMT5	physical	23749998
STK38_HUMAN	STK38	physical	23749998
MEP50_HUMAN	WDR77	physical	23749998
MED17_HUMAN	MED17	physical	23749998
MED12_HUMAN	MED12	physical	23749998
MED15_HUMAN	MED15	physical	23749998
CDK19_HUMAN	CDK19	physical	28514442
MED6_HUMAN	MED6	physical	28514442
MED8_HUMAN	MED8	physical	28514442
MED13_HUMAN	MED13	physical	28514442
MED24_HUMAN	MED24	physical	28514442
MD12L_HUMAN	MED12L	physical	28514442
MD13L_HUMAN	MED13L	physical	28514442
MED12_HUMAN	MED12	physical	28514442
MED17_HUMAN	MED17	physical	28514442
MED28_HUMAN	MED28	physical	28514442
MED14_HUMAN	MED14	physical	28514442
MED27_HUMAN	MED27	physical	28514442
MED10_HUMAN	MED10	physical	28514442
MED4_HUMAN	MED4	physical	28514442
MED9_HUMAN	MED9	physical	28514442
MED23_HUMAN	MED23	physical	28514442
MED30_HUMAN	MED30	physical	28514442
CDK16_HUMAN	CDK16	physical	28514442
MED22_HUMAN	MED22	physical	28514442
MED16_HUMAN	MED16	physical	28514442
MYPT1_HUMAN	PPP1R12A	physical	28514442
MED29_HUMAN	MED29	physical	28514442
MED15_HUMAN	MED15	physical	28514442
MED20_HUMAN	MED20	physical	28514442
MED25_HUMAN	MED25	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of CDK8_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-410 AND SER-413, ANDMASS SPECTROMETRY.	19369195 [Abstract]