dbPTM

SMAD1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SMAD1_HUMAN
UniProt AC	Q15797
Protein Name	Mothers against decapentaplegic homolog 1
Gene Name	SMAD1
Organism	Homo sapiens (Human).
Sequence Length	465
Subcellular Localization	Cytoplasm . Nucleus . Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4 (PubMed:15647271). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15647271). Exported from the nucleus to the cytoplasm when
Protein Description	Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression..
Protein Sequence	MNVTSLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQPMDTNMMAPPLPSEINRGDVQAVAYEEPKHWCSIVYYELNNRVGEAFHASSTSVLVDGFTDPSNNKNRFCLGLLSNVNRNSTIENTRRHIGKGVHLYYVGGEVYAECLSDSSIFVQSRNCNYHHGFHPTTVCKIPSGCSLKIFNNQEFAQLLAQSVNHGFETVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQMGSPHNPISSVS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MNVTSLFS -------CCCCCCCC	9.09	-
5	Phosphorylation	---MNVTSLFSFTSP ---CCCCCCCCCCCH	24.12	28348404
8	Phosphorylation	MNVTSLFSFTSPAVK CCCCCCCCCCCHHHH	32.67	26270265
10	Phosphorylation	VTSLFSFTSPAVKRL CCCCCCCCCHHHHHH	32.38	22199227
11	Phosphorylation	TSLFSFTSPAVKRLL CCCCCCCCHHHHHHH	14.62	22199227
21	Ubiquitination	VKRLLGWKQGDEEEK HHHHHCCCCCCHHHH	42.47	29967540
28	Ubiquitination	KQGDEEEKWAEKAVD CCCCHHHHHHHHHHH	55.79	27667366
32	Ubiquitination	EEEKWAEKAVDALVK HHHHHHHHHHHHHHH	46.06	29967540
62	Phosphorylation	LSCPGQPSNCVTIPR HCCCCCCCCEEEECC	34.67	24400094
78	Phosphorylation	LDGRLQVSHRKGLPH CCCCEEEECCCCCCE	12.47	26670566
81	Ubiquitination	RLQVSHRKGLPHVIY CEEEECCCCCCEEEE	60.42	33845483
88	Phosphorylation	KGLPHVIYCRVWRWP CCCCEEEEEEEEECC	3.52	-
116	Ubiquitination	CEFPFGSKQKEVCIN ECCCCCCCCCEEEEC	66.81	22817900
116	Sumoylation	CEFPFGSKQKEVCIN ECCCCCCCCCEEEEC	66.81	-
118	Ubiquitination	FPFGSKQKEVCINPY CCCCCCCCEEEECCC	56.71	22817900
118	Sumoylation	FPFGSKQKEVCINPY CCCCCCCCEEEECCC	56.71	-
127	Phosphorylation	VCINPYHYKRVESPV EEECCCCCCCCCCCC	8.47	12601080
132	Phosphorylation	YHYKRVESPVLPPVL CCCCCCCCCCCCCEE	19.90	30266825
144	Phosphorylation	PVLVPRHSEYNPQHS CEECCCCCCCCCCCC	42.95	24719451
146	Phosphorylation	LVPRHSEYNPQHSLL ECCCCCCCCCCCCHH	34.85	27282143
151	Phosphorylation	SEYNPQHSLLAQFRN CCCCCCCCHHHHHHH	21.60	29214152
187	Phosphorylation	NSHPFPHSPNSSYPN CCCCCCCCCCCCCCC	26.47	22817900
195	Phosphorylation	PNSSYPNSPGSSSST CCCCCCCCCCCCCCC	26.57	22817900
202	Phosphorylation	SPGSSSSTYPHSPTS CCCCCCCCCCCCCCC	42.91	22817900
206	Phosphorylation	SSSTYPHSPTSSDPG CCCCCCCCCCCCCCC	25.93	22817900
214	Phosphorylation	PTSSDPGSPFQMPAD CCCCCCCCCCCCCCC	28.12	22817900
239	Phosphorylation	DPMTQDGSQPMDTNM CCCCCCCCCCCCCCC	39.12	22588298
265	Phosphorylation	GDVQAVAYEEPKHWC CCEEEEEECCCCCEE	17.77	27642862
269	Ubiquitination	AVAYEEPKHWCSIVY EEEECCCCCEEEEEE	53.68	22817900
315	Phosphorylation	RFCLGLLSNVNRNST CEEHHHHCCCCCCCC	43.72	21712546
321	Phosphorylation	LSNVNRNSTIENTRR HCCCCCCCCCHHHHH	27.76	28348404
322	Phosphorylation	SNVNRNSTIENTRRH CCCCCCCCCHHHHHH	35.54	22985185
337	Phosphorylation	IGKGVHLYYVGGEVY CCCCEEEEEECCEEE	4.92	22468782
338	Phosphorylation	GKGVHLYYVGGEVYA CCCEEEEEECCEEEE	10.05	22468782
344	Phosphorylation	YYVGGEVYAECLSDS EEECCEEEEECCCCC	7.74	22468782
418	Ubiquitination	TIRMSFVKGWGAEYH CEEHHHHCCCCCCHH	47.11	21890473
452	Phosphorylation	QWLDKVLTQMGSPHN HHHHHHHHHCCCCCC	21.35	25159151
456	Phosphorylation	KVLTQMGSPHNPISS HHHHHCCCCCCCCCC	20.04	25159151
462	Phosphorylation	GSPHNPISSVS---- CCCCCCCCCCC----	26.41	22322096
463	Phosphorylation	SPHNPISSVS----- CCCCCCCCCC-----	27.40	22322096
465	Phosphorylation	HNPISSVS------- CCCCCCCC-------	36.13	22322096

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
187	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
187	S	Phosphorylation	Kinase	CDK8	P49336	PSP
187	S	Phosphorylation	Kinase	CDK9	P50750	PSP
195	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
195	S	Phosphorylation	Kinase	CDK8	P49336	PSP
195	S	Phosphorylation	Kinase	CDK9	P50750	PSP
202	T	Phosphorylation	Kinase	GSK3B	P49841	PSP
206	S	Phosphorylation	Kinase	CDK7	P50613	PSP
206	S	Phosphorylation	Kinase	CDK8	P49336	PSP
206	S	Phosphorylation	Kinase	CDK9	P50750	PSP
206	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
214	S	Phosphorylation	Kinase	CDK8	P49336	PSP
214	S	Phosphorylation	Kinase	CDK9	P50750	PSP
214	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
239	S	Phosphorylation	Kinase	ATM	Q13315	PSP
322	T	Phosphorylation	Kinase	TNIK	Q9UKE5	Uniprot
322	T	Phosphorylation	Kinase	MINK	Q8N4C8	PSP
322	T	Phosphorylation	Kinase	HGK	O95819	PSP
462	S	Phosphorylation	Kinase	BMR1B	O00238	PhosphoELM
463	S	Phosphorylation	Kinase	BMR1B	O00238	PhosphoELM
465	S	Phosphorylation	Kinase	BMR1B	O00238	PhosphoELM
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF1	Q9HCE7	PMID:14711801
-	K	Ubiquitination	E3 ubiquitin ligase	STUB1	Q9UNE7	PMID:14701756
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF2	Q9HAU4	PMID:11158580

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SMAD1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SMAD1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HIPK2_HUMAN	HIPK2	physical	12874272
SKI_HUMAN	SKI	physical	12874272
SMAD4_HUMAN	SMAD4	physical	12874272
DVL1_HUMAN	DVL1	physical	12650946
ERBIN_HUMAN	ERBB2IP	physical	12650946
NGN1_HUMAN	NEUROG1	physical	11239394
SNIP1_HUMAN	SNIP1	physical	10887155
EP300_HUMAN	EP300	physical	10205054
FOXG1_HUMAN	FOXG1	physical	11387330
PSB4_HUMAN	PSMB4	physical	12097147
RUNX2_HUMAN	RUNX2	physical	10962029
SMUF2_HUMAN	SMURF2	physical	11016919
HXC8_HUMAN	HOXC8	physical	10224145
RUNX1_HUMAN	RUNX1	physical	10531362
RUNX2_HUMAN	RUNX2	physical	10531362
RUNX3_HUMAN	RUNX3	physical	10531362
PSB4_HUMAN	PSMB4	physical	11438941
OAZ3_HUMAN	OAZ3	physical	11438941
CASL_HUMAN	NEDD9	physical	11118211
SMAD4_HUMAN	SMAD4	physical	11700304
SMAD6_HUMAN	SMAD6	physical	9256479
TCF20_HUMAN	TCF20	physical	20211142
CREST_HUMAN	SS18L1	physical	20211142
WWP1_HUMAN	WWP1	physical	15221015
SMUF1_HUMAN	SMURF1	physical	15221015
SMUF2_HUMAN	SMURF2	physical	15221015
NKX32_HUMAN	NKX3-2	physical	14612411
PIAS4_HUMAN	PIAS4	physical	12904571
TYY1_HUMAN	YY1	physical	12808092
MED15_HUMAN	MED15	physical	12167862
MED24_HUMAN	MED24	physical	12167862
MED6_HUMAN	MED6	physical	12167862
SMAD4_HUMAN	SMAD4	physical	12167862
EVI1_HUMAN	MECOM	physical	15849193
KAT2A_HUMAN	KAT2A	physical	15009097
PSB4_HUMAN	PSMB4	physical	11571290
OAZ1_HUMAN	OAZ1	physical	11571290
SKI_HUMAN	SKI	physical	14699069
HDAC1_HUMAN	HDAC1	physical	14699069
SMUF2_HUMAN	SMURF2	physical	11158580
SMUF1_HUMAN	SMURF1	physical	17289590
NU214_HUMAN	NUP214	physical	17289590
FOXO3_HUMAN	FOXO3	physical	17289590
CHIP_HUMAN	STUB1	physical	21454478
UCHL3_HUMAN	UCHL3	physical	21453705
SMAD4_HUMAN	SMAD4	physical	14701756
SMUF1_HUMAN	SMURF1	physical	14701756
SMUF2_HUMAN	SMURF2	physical	14701756
CHIP_HUMAN	STUB1	physical	14701756
USP9X_HUMAN	USP9X	physical	19135894
SKI_HUMAN	SKI	physical	12426322
SKIL_HUMAN	SKIL	physical	12426322
MEN1_HUMAN	MEN1	physical	12649288
NEDD4_HUMAN	NEDD4	physical	21308777
UBP15_HUMAN	USP15	physical	21947082
CDK8_HUMAN	CDK8	physical	19914168
YAP1_HUMAN	YAP1	physical	19914168
SMAD6_HUMAN	SMAD6	physical	12857866
SMUF1_HUMAN	SMURF1	physical	21685363
YAP1_HUMAN	YAP1	physical	21685363
SMUF1_HUMAN	SMURF1	physical	19917253
UBP15_HUMAN	USP15	physical	22344298
HGS_HUMAN	HGS	physical	11094085
ZCH12_HUMAN	ZCCHC12	physical	19416967
PAX6_HUMAN	PAX6	physical	17251190
SMAD6_HUMAN	SMAD6	physical	9436979
SMAD4_HUMAN	SMAD4	physical	9436979
PPM1A_HUMAN	PPM1A	physical	22588298
P53_HUMAN	TP53	physical	22588298
A4_HUMAN	APP	physical	21832049
SMAD1_HUMAN	SMAD1	physical	9111321
SMAD2_HUMAN	SMAD2	physical	9111321
SMAD3_HUMAN	SMAD3	physical	9111321
SMAD4_HUMAN	SMAD4	physical	9111321
PSD11_HUMAN	PSMD11	physical	15231748
KMT2D_HUMAN	KMT2D	physical	15231748
MAST4_HUMAN	MAST4	physical	15231748
ZEB2_HUMAN	ZEB2	physical	15231748
HBP1_HUMAN	HBP1	physical	15231748
SF3B1_HUMAN	SF3B1	physical	15231748
PIAS1_HUMAN	PIAS1	physical	15231748
AP2A2_HUMAN	AP2A2	physical	15231748
RU17_HUMAN	SNRNP70	physical	15231748
ALDR_HUMAN	AKR1B1	physical	15231748
RFX1_HUMAN	RFX1	physical	15231748
PSB4_HUMAN	PSMB4	physical	15231748
SOX5_HUMAN	SOX5	physical	15231748
ZNF76_HUMAN	ZNF76	physical	15231748
NEDD4_HUMAN	NEDD4	physical	15231748
XPC_HUMAN	XPC	physical	15231748
NOTC2_HUMAN	NOTCH2	physical	15231748
SMAD4_HUMAN	SMAD4	physical	15231748
CASL_HUMAN	NEDD9	physical	15231748
PUM1_HUMAN	PUM1	physical	15231748
TTF1_HUMAN	TTF1	physical	15231748
SMAD1_HUMAN	SMAD1	physical	15231748
CAMP1_HUMAN	CAMSAP1	physical	15231748
I2BP1_HUMAN	IRF2BP1	physical	15231748
MGAP_HUMAN	MGA	physical	15231748
PIAS4_HUMAN	PIAS4	physical	15231748
ZSCA4_HUMAN	ZSCAN4	physical	15231748
SMCE1_HUMAN	SMARCE1	physical	15231748
ANR27_HUMAN	ANKRD27	physical	15231748
INP4A_HUMAN	INPP4A	physical	15231748
PSMD1_HUMAN	PSMD1	physical	15231748
SMAD5_HUMAN	SMAD5	physical	15231748
PIGQ_HUMAN	PIGQ	physical	15231748
ZN251_HUMAN	ZNF251	physical	15231748
SMUF2_HUMAN	SMURF2	physical	15231748
SMUF1_HUMAN	SMURF1	physical	15231748
ZDHC3_HUMAN	ZDHHC3	physical	15231748
MKL2_HUMAN	MKL2	physical	15231748
TTF2_HUMAN	TTF2	physical	15231748
ICK_HUMAN	ICK	physical	15231748
ZN510_HUMAN	ZNF510	physical	15231748
MAN1_HUMAN	LEMD3	physical	15231748
CHMP3_HUMAN	CHMP3	physical	15231748
STA13_HUMAN	STARD13	physical	15231748
GMEB1_HUMAN	GMEB1	physical	15231748
MAN1_HUMAN	LEMD3	physical	15489854
SMAD4_HUMAN	SMAD4	physical	17183365
ANDR_HUMAN	AR	physical	17183365
UBC_HUMAN	UBC	physical	11438941
SMUF1_HUMAN	SMURF1	physical	24828823
MEP50_HUMAN	WDR77	physical	23734213
ANDR_HUMAN	AR	physical	23734213
LEF1_HUMAN	LEF1	physical	15750622
PARD3_HUMAN	PARD3	physical	12650946
GSK3B_HUMAN	GSK3B	physical	25241761
GLI3_HUMAN	GLI3	physical	25241761
SMAD7_HUMAN	SMAD7	physical	26555259
SMAD4_HUMAN	SMAD4	physical	26555259
SMUF1_HUMAN	SMURF1	physical	28881580
SMAD4_HUMAN	SMAD4	physical	28468752
SMUF2_HUMAN	SMURF2	physical	28468752

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SMAD1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"The TGF-beta family mediator Smad1 is phosphorylated directly andactivated functionally by the BMP receptor kinase."; Kretzschmar M., Liu F., Hata A., Doody J., Massague J.; Genes Dev. 11:984-995(1997). Cited for: PHOSPHORYLATION AT SER-463 AND SER-465.	9136927 [Abstract]
"Smad inhibition by the Ste20 kinase Misshapen."; Kaneko S., Chen X., Lu P., Yao X., Wright T.G., Rajurkar M.,Kariya K., Mao J., Ip Y.T., Xu L.; Proc. Natl. Acad. Sci. U.S.A. 108:11127-11132(2011). Cited for: PHOSPHORYLATION AT THR-322.	21690388 [Abstract]