dbPTM

MAN1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	MAN1_HUMAN
UniProt AC	Q9Y2U8
Protein Name	Inner nuclear membrane protein Man1
Gene Name	LEMD3
Organism	Homo sapiens (Human).
Sequence Length	911
Subcellular Localization	Nucleus inner membrane Multi-pass membrane protein .
Protein Description	Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest..
Protein Sequence	MAAAAASAPQQLSDEELFSQLRRYGLSPGPVTESTRPVYLKKLKKLREEEQQQHRSGGRGNKTRNSNNNNTAAATVAAAGPAAAAAAGMGVRPVSGDLSYLRTPGGLCRISASGPESLLGGPGGASAAPAAGSKVLLGFSSDESDVEASPRDQAGGGGRKDRASLQYRGLKAPPAPLAASEVTNSNSAERRKPHSWWGARRPAGPELQTPPGKDGAVEDEEGEGEDGEERDPETEEPLWASRTVNGSRLVPYSCRENYSDSEEEDDDDVASSRQVLKDDSLSRHRPRRTHSKPLPPLTAKSAGGRLETSVQGGGGLAMNDRAAAAGSLDRSRNLEEAAAAEQGGGCDQVDSSPVPRYRVNAKKLTPLLPPPLTDMDSTLDSSTGSLLKTNNHIGGGAFSVDSPRIYSNSLPPSAAVAASSSLRINHANHTGSNHTYLKNTYNKPKLSEPEEELLQQFKREEVSPTGSFSAHYLSMFLLTAACLFFLILGLTYLGMRGTGVSEDGELSIENPFGETFGKIQESEKTLMMNTLYKLHDRLAQLAGDHECGSSSQRTLSVQEAAAYLKDLGPEYEGIFNTSLQWILENGKDVGIRCVGFGPEEELTNITDVQFLQSTRPLMSFWCRFRRAFVTVTHRLLLLCLGVVMVCVVLRYMKYRWTKEEEETRQMYDMVVKIIDVLRSHNEACQENKDLQPYMPIPHVRDSLIQPHDRKKMKKVWDRAVDFLAANESRVRTETRRIGGADFLVWRWIQPSASCDKILVIPSKVWQGQAFHLDRRNSPPNSLTPCLKIRNMFDPVMEIGDQWHLAIQEAILEKCSDNDGIVHIAVDKNSREGCVYVKCLSPEYAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTSNTPLKPSNKHMNSMSHLRLRTGLTNSQGSS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAAAASAP ------CCHHHCCCC	13.05	22814378
7	Phosphorylation	-MAAAAASAPQQLSD -CCHHHCCCCCCCCH	35.44	22199227
13	Phosphorylation	ASAPQQLSDEELFSQ CCCCCCCCHHHHHHH	38.34	22199227
19	Phosphorylation	LSDEELFSQLRRYGL CCHHHHHHHHHHHCC	40.71	28464451
24	Phosphorylation	LFSQLRRYGLSPGPV HHHHHHHHCCCCCCC	18.91	21712546
27	Phosphorylation	QLRRYGLSPGPVTES HHHHHCCCCCCCCCC	24.37	25159151
32	Phosphorylation	GLSPGPVTESTRPVY CCCCCCCCCCCCHHH	27.61	25159151
34	Phosphorylation	SPGPVTESTRPVYLK CCCCCCCCCCHHHHH	21.98	22199227
35	Phosphorylation	PGPVTESTRPVYLKK CCCCCCCCCHHHHHH	32.53	22199227
39	Phosphorylation	TESTRPVYLKKLKKL CCCCCHHHHHHHHHH	18.58	29396449
41	Malonylation	STRPVYLKKLKKLRE CCCHHHHHHHHHHHH	38.07	26320211
41	Ubiquitination	STRPVYLKKLKKLRE CCCHHHHHHHHHHHH	38.07	-
56	O-linked_Glycosylation	EEQQQHRSGGRGNKT HHHHHHHCCCCCCCC	43.40	30379171
95	Phosphorylation	GMGVRPVSGDLSYLR CCCCEECCCCCCCCC	29.31	21712546
99	Phosphorylation	RPVSGDLSYLRTPGG EECCCCCCCCCCCCC	27.19	24719451
103	Phosphorylation	GDLSYLRTPGGLCRI CCCCCCCCCCCEEEE	24.56	21815630
111	Phosphorylation	PGGLCRISASGPESL CCCEEEEECCCCHHH	9.31	25850435
113	Phosphorylation	GLCRISASGPESLLG CEEEEECCCCHHHCC	48.35	25850435
117	Phosphorylation	ISASGPESLLGGPGG EECCCCHHHCCCCCC	31.84	25850435
126	Phosphorylation	LGGPGGASAAPAAGS CCCCCCHHCCCCCCC	28.39	28857561
133	Phosphorylation	SAAPAAGSKVLLGFS HCCCCCCCEEEEECC	18.18	28857561
140	Phosphorylation	SKVLLGFSSDESDVE CEEEEECCCCHHHCC	34.77	29255136
141	Phosphorylation	KVLLGFSSDESDVEA EEEEECCCCHHHCCC	43.04	29255136
144	Phosphorylation	LGFSSDESDVEASPR EECCCCHHHCCCCCC	52.53	19664994
149	Phosphorylation	DESDVEASPRDQAGG CHHHCCCCCCCCCCC	13.63	30266825
164	Phosphorylation	GGRKDRASLQYRGLK CCCCCCHHHEECCCC	19.52	23401153
167	Phosphorylation	KDRASLQYRGLKAPP CCCHHHEECCCCCCC	16.37	-
180	Phosphorylation	PPAPLAASEVTNSNS CCCCCCCHHCCCCCC	27.30	29978859
183	Phosphorylation	PLAASEVTNSNSAER CCCCHHCCCCCCCHH	28.95	30266825
185	Phosphorylation	AASEVTNSNSAERRK CCHHCCCCCCCHHCC	23.85	30266825
187	Phosphorylation	SEVTNSNSAERRKPH HHCCCCCCCHHCCCC	31.18	29255136
209	Phosphorylation	PAGPELQTPPGKDGA CCCCCCCCCCCCCCC	44.04	25159151
213	Ubiquitination	ELQTPPGKDGAVEDE CCCCCCCCCCCCCCC	59.78	-
243	Phosphorylation	EPLWASRTVNGSRLV CCCEEECCCCCCCCE	18.81	28555341
247	Phosphorylation	ASRTVNGSRLVPYSC EECCCCCCCCEEEEC	19.76	23898821
252	Phosphorylation	NGSRLVPYSCRENYS CCCCCEEEECCCCCC	16.96	22115753
253	Phosphorylation	GSRLVPYSCRENYSD CCCCEEEECCCCCCC	10.71	28450419
258	Phosphorylation	PYSCRENYSDSEEED EEECCCCCCCCCCCC	14.76	22167270
259	Phosphorylation	YSCRENYSDSEEEDD EECCCCCCCCCCCCC	46.36	22167270
261	Phosphorylation	CRENYSDSEEEDDDD CCCCCCCCCCCCCCC	40.75	22167270
271	Phosphorylation	EDDDDVASSRQVLKD CCCCCHHHHHHHHHC	26.38	23927012
272	Phosphorylation	DDDDVASSRQVLKDD CCCCHHHHHHHHHCC	19.59	23927012
277	Ubiquitination	ASSRQVLKDDSLSRH HHHHHHHHCCCHHCC	61.71	-
280	Phosphorylation	RQVLKDDSLSRHRPR HHHHHCCCHHCCCCC	38.08	23401153
282	Phosphorylation	VLKDDSLSRHRPRRT HHHCCCHHCCCCCCC	29.96	30266825
289	Phosphorylation	SRHRPRRTHSKPLPP HCCCCCCCCCCCCCC	31.61	27732954
291	Phosphorylation	HRPRRTHSKPLPPLT CCCCCCCCCCCCCCC	35.04	27732954
298	Phosphorylation	SKPLPPLTAKSAGGR CCCCCCCCCCCCCCC	37.50	23186163
300	Ubiquitination	PLPPLTAKSAGGRLE CCCCCCCCCCCCCEE	35.29	-
301	Phosphorylation	LPPLTAKSAGGRLET CCCCCCCCCCCCEEE	29.67	28555341
308	Phosphorylation	SAGGRLETSVQGGGG CCCCCEEEEEECCCC	38.50	23186163
309	Phosphorylation	AGGRLETSVQGGGGL CCCCEEEEEECCCCC	11.76	21815630
327	Phosphorylation	DRAAAAGSLDRSRNL HHHHHHCCCCCCCCH	23.52	25159151
331	Phosphorylation	AAGSLDRSRNLEEAA HHCCCCCCCCHHHHH	25.78	25159151
351	Phosphorylation	GGCDQVDSSPVPRYR CCCCCCCCCCCCCCE	37.37	30278072
352	Phosphorylation	GCDQVDSSPVPRYRV CCCCCCCCCCCCCEE	26.00	30278072
365	Phosphorylation	RVNAKKLTPLLPPPL EECCCCCCCCCCCCC	22.29	29255136
373	Phosphorylation	PLLPPPLTDMDSTLD CCCCCCCCCCCCCCC	34.74	20068231
377	Phosphorylation	PPLTDMDSTLDSSTG CCCCCCCCCCCCCCC	25.00	21712546
378	Phosphorylation	PLTDMDSTLDSSTGS CCCCCCCCCCCCCCC	30.25	20068231
381	Phosphorylation	DMDSTLDSSTGSLLK CCCCCCCCCCCCEEE	32.67	20068231
382	Phosphorylation	MDSTLDSSTGSLLKT CCCCCCCCCCCEEEC	36.13	25159151
383	Phosphorylation	DSTLDSSTGSLLKTN CCCCCCCCCCEEECC	34.44	20068231
385	Phosphorylation	TLDSSTGSLLKTNNH CCCCCCCCEEECCCC	30.53	20068231
389	Phosphorylation	STGSLLKTNNHIGGG CCCCEEECCCCCCCC	41.02	20068231
399	Phosphorylation	HIGGGAFSVDSPRIY CCCCCCEECCCCCEE	25.20	25159151
402	Phosphorylation	GGAFSVDSPRIYSNS CCCEECCCCCEECCC	17.58	25159151
406	Phosphorylation	SVDSPRIYSNSLPPS ECCCCCEECCCCCHH	11.77	22210691
407	Phosphorylation	VDSPRIYSNSLPPSA CCCCCEECCCCCHHH	19.99	25159151
409	Phosphorylation	SPRIYSNSLPPSAAV CCCEECCCCCHHHHH	35.99	25627689
413	Phosphorylation	YSNSLPPSAAVAASS ECCCCCHHHHHHHCC	27.24	25627689
419	Phosphorylation	PSAAVAASSSLRINH HHHHHHHCCCCEEEC	15.66	28555341
420	Phosphorylation	SAAVAASSSLRINHA HHHHHHCCCCEEECC	28.76	25159151
421	Phosphorylation	AAVAASSSLRINHAN HHHHHCCCCEEECCC	21.12	25627689
430	Phosphorylation	RINHANHTGSNHTYL EEECCCCCCCCCCEE	41.95	29214152
432	Phosphorylation	NHANHTGSNHTYLKN ECCCCCCCCCCEEEC	26.96	22210691
436	Phosphorylation	HTGSNHTYLKNTYNK CCCCCCCEEECCCCC	14.23	28555341
438	Ubiquitination	GSNHTYLKNTYNKPK CCCCCEEECCCCCCC	36.51	-
441	Phosphorylation	HTYLKNTYNKPKLSE CCEEECCCCCCCCCC	30.49	21712546
443	Acetylation	YLKNTYNKPKLSEPE EEECCCCCCCCCCCH	32.87	30590259
445	Acetylation	KNTYNKPKLSEPEEE ECCCCCCCCCCCHHH	67.27	30590265
445	Ubiquitination	KNTYNKPKLSEPEEE ECCCCCCCCCCCHHH	67.27	-
447	Phosphorylation	TYNKPKLSEPEEELL CCCCCCCCCCHHHHH	58.18	25159151
458	Ubiquitination	EELLQQFKREEVSPT HHHHHHHHHHCCCCC	55.34	21906983
463	Phosphorylation	QFKREEVSPTGSFSA HHHHHCCCCCCCHHH	21.74	22817900
472	Phosphorylation	TGSFSAHYLSMFLLT CCCHHHHHHHHHHHH	10.53	30387612
491	Phosphorylation	FFLILGLTYLGMRGT HHHHHHHHHHCCCCC	18.20	30387612
524	Ubiquitination	GKIQESEKTLMMNTL CHHHHHHHHHHHHHH	57.12	21906983
533	Ubiquitination	LMMNTLYKLHDRLAQ HHHHHHHHHHHHHHH	42.32	-
537	Methylation	TLYKLHDRLAQLAGD HHHHHHHHHHHHHCC	22.60	115482519
630	Phosphorylation	RFRRAFVTVTHRLLL HHHHHHHHHHHHHHH	17.15	-
632	Phosphorylation	RRAFVTVTHRLLLLC HHHHHHHHHHHHHHH	7.60	-
651	Phosphorylation	MVCVVLRYMKYRWTK HHHHHHHHHHHCCCC	8.19	-
679	Phosphorylation	KIIDVLRSHNEACQE HHHHHHHHCHHHHHH	26.89	-
688	Acetylation	NEACQENKDLQPYMP HHHHHHCCCCCCCCC	60.09	26051181
688	Ubiquitination	NEACQENKDLQPYMP HHHHHHCCCCCCCCC	60.09	-
728	Phosphorylation	DFLAANESRVRTETR HHHHHCHHHCHHCCC	35.56	23312004
751	Phosphorylation	VWRWIQPSASCDKIL EEEECCCCCCCCEEE	19.21	21815630
753	Phosphorylation	RWIQPSASCDKILVI EECCCCCCCCEEEEE	27.47	21815630
756	Acetylation	QPSASCDKILVIPSK CCCCCCCEEEEEECH	42.12	26051181
756	Ubiquitination	QPSASCDKILVIPSK CCCCCCCEEEEEECH	42.12	-
762	Phosphorylation	DKILVIPSKVWQGQA CEEEEEECHHHCCCC	28.81	24719451
763	Ubiquitination	KILVIPSKVWQGQAF EEEEEECHHHCCCCC	41.98	-
776	Phosphorylation	AFHLDRRNSPPNSLT CCCCCCCCCCCCCCC	60.81	24719451
777	Phosphorylation	FHLDRRNSPPNSLTP CCCCCCCCCCCCCCH	39.66	30266825
781	Phosphorylation	RRNSPPNSLTPCLKI CCCCCCCCCCHHHHH	39.12	30266825
783	Phosphorylation	NSPPNSLTPCLKIRN CCCCCCCCHHHHHHH	16.27	30266825
787	Acetylation	NSLTPCLKIRNMFDP CCCCHHHHHHHHCCC	46.46	26051181
787	Malonylation	NSLTPCLKIRNMFDP CCCCHHHHHHHHCCC	46.46	26320211
787	Ubiquitination	NSLTPCLKIRNMFDP CCCCHHHHHHHHCCC	46.46	-
827	Ubiquitination	IVHIAVDKNSREGCV EEEEEECCCCCCCEE	50.66	21906983
837	Acetylation	REGCVYVKCLSPEYA CCCEEEEEECCHHHC	16.27	26051181
837	Ubiquitination	REGCVYVKCLSPEYA CCCEEEEEECCHHHC	16.27	-
840	Phosphorylation	CVYVKCLSPEYAGKA EEEEEECCHHHCHHH	25.75	22199227
843	Phosphorylation	VKCLSPEYAGKAFKA EEECCHHHCHHHHHH	24.84	29759185
849	Malonylation	EYAGKAFKALHGSWF HHCHHHHHHHHCCEE	55.70	26320211
849	Ubiquitination	EYAGKAFKALHGSWF HHCHHHHHHHHCCEE	55.70	-
864	Acetylation	DGKLVTVKYLRLDRY CCEEEEEEEEEECCC	29.24	26051181
864	Malonylation	DGKLVTVKYLRLDRY CCEEEEEEEEEECCC	29.24	26320211
880	Phosphorylation	HRFPQALTSNTPLKP HHCCHHHHCCCCCCC	23.97	30266825
881	Phosphorylation	RFPQALTSNTPLKPS HCCHHHHCCCCCCCC	39.29	30266825
882	Phosphorylation	FPQALTSNTPLKPSN CCHHHHCCCCCCCCC	40.24	24719451
883	Phosphorylation	PQALTSNTPLKPSNK CHHHHCCCCCCCCCC	29.75	30266825
888	Phosphorylation	SNTPLKPSNKHMNSM CCCCCCCCCCCCCCC	57.85	30266825
894	Phosphorylation	PSNKHMNSMSHLRLR CCCCCCCCCCCHHHH	17.68	-
895	Phosphorylation	SNKHMNSMSHLRLRT CCCCCCCCCCHHHHH	2.19	24719451
896	Phosphorylation	NKHMNSMSHLRLRTG CCCCCCCCCHHHHHC	21.13	21857030
902	Phosphorylation	MSHLRLRTGLTNSQG CCCHHHHHCCCCCCC	40.93	22199227
905	Phosphorylation	LRLRTGLTNSQGSS- HHHHHCCCCCCCCC-	33.88	22199227
907	Phosphorylation	LRTGLTNSQGSS--- HHHCCCCCCCCC---	30.59	26055452
910	Phosphorylation	GLTNSQGSS------ CCCCCCCCC------	25.82	22199227
911	Phosphorylation	LTNSQGSS------- CCCCCCCC-------	51.57	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of MAN1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of MAN1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of MAN1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
Oops, there are no PPI records of MAN1_HUMAN !!

Drug and Disease Associations

Kegg Disease
H00452	Disorders caused by loss-of-function of LEMD3, including: Osteopoikilosis; Buschke-Ollendorff syndro
OMIM Disease
166700	Buschke-Ollendorff syndrome (BOS)
155950	Melorheostosis (MEL)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of MAN1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140; SER-141; SER-144AND SER-261, AND MASS SPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185; SER-187; SER-259;SER-261; SER-280; SER-352; THR-365; SER-377; SER-402; SER-777 ANDSER-911, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187 AND SER-402, ANDMASS SPECTROMETRY.	18220336 [Abstract]
"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."; Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.; J. Proteome Res. 6:4150-4162(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-331, AND MASSSPECTROMETRY.	17924679 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-259 AND SER-261, ANDMASS SPECTROMETRY.	17081983 [Abstract]