dbPTM

PSB4_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PSB4_HUMAN
UniProt AC	P28070
Protein Name	Proteasome subunit beta type-4
Gene Name	PSMB4
Organism	Homo sapiens (Human).
Sequence Length	264
Subcellular Localization	Cytoplasm . Nucleus .
Protein Description	Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1..
Protein Sequence	MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSTETNWDIAHMISGFE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MEAFLGSR -------CCCCCCCC	8.27	22223895
9	Phosphorylation	EAFLGSRSGLWAGGP CCCCCCCCCCCCCCC	39.68	24114839
22	Phosphorylation	GPAPGQFYRIPSTPD CCCCCCEEECCCCCC	10.35	20068231
26	Phosphorylation	GQFYRIPSTPDSFMD CCEEECCCCCCCCCC	50.64	25106551
27	Phosphorylation	QFYRIPSTPDSFMDP CEEECCCCCCCCCCH	26.26	25106551
30	Phosphorylation	RIPSTPDSFMDPASA ECCCCCCCCCCHHHH	24.55	28450419
44	Phosphorylation	ALYRGPITRTQNPMV HHHCCCCCCCCCCCC	30.69	22210691
46	Phosphorylation	YRGPITRTQNPMVTG HCCCCCCCCCCCCCC	24.85	20068231
50	Sulfoxidation	ITRTQNPMVTGTSVL CCCCCCCCCCCEEEE	5.74	21406390
52	Phosphorylation	RTQNPMVTGTSVLGV CCCCCCCCCEEEEEE	28.61	21406692
54	Phosphorylation	QNPMVTGTSVLGVKF CCCCCCCEEEEEEEE	13.29	20068231
55	Phosphorylation	NPMVTGTSVLGVKFE CCCCCCEEEEEEEEC	19.38	20068231
74	Phosphorylation	IAADMLGSYGSLARF EEEEHHCCCHHHHHH	23.51	28348404
75	Phosphorylation	AADMLGSYGSLARFR EEEHHCCCHHHHHHH	14.67	20090780
77	Phosphorylation	DMLGSYGSLARFRNI EHHCCCHHHHHHHCC	15.60	28348404
85	Phosphorylation	LARFRNISRIMRVNN HHHHHCCHHHEEECC	21.21	24719451
93	Phosphorylation	RIMRVNNSTMLGASG HHEEECCCCCCCCCC	15.25	21712546
94	Phosphorylation	IMRVNNSTMLGASGD HEEECCCCCCCCCCC	20.66	24719451
99	Phosphorylation	NSTMLGASGDYADFQ CCCCCCCCCCHHHHH	30.33	28102081
102	Phosphorylation	MLGASGDYADFQYLK CCCCCCCHHHHHHHH	16.17	7918633
107	Phosphorylation	GDYADFQYLKQVLGQ CCHHHHHHHHHHHCC	18.83	20090780
109	Sumoylation	YADFQYLKQVLGQMV HHHHHHHHHHHCCHH	33.17	-
109	Ubiquitination	YADFQYLKQVLGQMV HHHHHHHHHHHCCHH	33.17	-
115	Sulfoxidation	LKQVLGQMVIDEELL HHHHHCCHHCCHHHC	2.43	30846556
127	Phosphorylation	ELLGDGHSYSPRAIH HHCCCCCCCCHHHHH	32.60	24719451
128	Phosphorylation	LLGDGHSYSPRAIHS HCCCCCCCCHHHHHH	19.61	27080861
129	Phosphorylation	LGDGHSYSPRAIHSW CCCCCCCCHHHHHHH	15.78	27282143
147	Ubiquitination	AMYSRRSKMNPLWNT HHHHCHHHCCCCCCE	40.67	-
186	Phosphorylation	APSLATGYGAYLAQP CCCHHCCHHHHHHHH	8.33	-
189	Phosphorylation	LATGYGAYLAQPLLR HHCCHHHHHHHHHHH	9.75	-
201	Acetylation	LLREVLEKQPVLSQT HHHHHHHHCCCCCHH	56.60	23236377
201	Ubiquitination	LLREVLEKQPVLSQT HHHHHHHHCCCCCHH	56.60	21906983
206	Phosphorylation	LEKQPVLSQTEARDL HHHCCCCCHHHHHHH	34.98	23663014
208	Phosphorylation	KQPVLSQTEARDLVE HCCCCCHHHHHHHHH	28.39	23663014
222	Phosphorylation	ERCMRVLYYRDARSY HHHHHHHHHHCCCCC	8.13	28152594
223	Phosphorylation	RCMRVLYYRDARSYN HHHHHHHHHCCCCCC	9.61	28152594
228	Phosphorylation	LYYRDARSYNRFQIA HHHHCCCCCCCEEEE	28.60	-
236	Phosphorylation	YNRFQIATVTEKGVE CCCEEEEEEECCCEE	29.59	-
238	Phosphorylation	RFQIATVTEKGVEIE CEEEEEEECCCEEEE	27.85	-
240	Ubiquitination	QIATVTEKGVEIEGP EEEEEECCCEEEECC	59.91	21906983
249	Phosphorylation	VEIEGPLSTETNWDI EEEECCCCCCCCHHH	27.60	22817900
250	Phosphorylation	EIEGPLSTETNWDIA EEECCCCCCCCHHHH	55.29	28348404
252	Phosphorylation	EGPLSTETNWDIAHM ECCCCCCCCHHHHHH	41.52	28348404
259	Sulfoxidation	TNWDIAHMISGFE-- CCHHHHHHHHCCC--	1.60	30846556
261	Phosphorylation	WDIAHMISGFE---- HHHHHHHHCCC----	29.83	27251275

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of PSB4_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PSB4_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PSB4_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
OAZ1_HUMAN	OAZ1	physical	11571290
PRP19_HUMAN	PRPF19	physical	15660529
NUB1_HUMAN	NUB1	physical	16707496
UBD_HUMAN	UBD	physical	16707496
PRS8_HUMAN	PSMC5	physical	16707496
PSB3_HUMAN	PSMB3	physical	17948026
PSB5_HUMAN	PSMB5	physical	17948026
PSB7_HUMAN	PSMB7	physical	17948026
PSB8_HUMAN	PSMB8	physical	17948026
POMP_HUMAN	POMP	physical	17948026
PSB10_HUMAN	PSMB10	physical	17948026
CRBN_HUMAN	CRBN	physical	23026050
A4_HUMAN	APP	physical	21832049
PSB5_HUMAN	PSMB5	physical	22939629
PSB6_HUMAN	PSMB6	physical	22939629
PSB7_HUMAN	PSMB7	physical	22939629
PSB8_HUMAN	PSMB8	physical	22939629
PSMD7_HUMAN	PSMD7	physical	22939629
PSMD1_HUMAN	PSMD1	physical	22939629
PSMD2_HUMAN	PSMD2	physical	22939629
PSMD3_HUMAN	PSMD3	physical	22939629
PSMD8_HUMAN	PSMD8	physical	22939629
PSMD4_HUMAN	PSMD4	physical	22939629
PSMD6_HUMAN	PSMD6	physical	22939629
PSDE_HUMAN	PSMD14	physical	22939629
PSD13_HUMAN	PSMD13	physical	22939629
PSD11_HUMAN	PSMD11	physical	22939629
PSME3_HUMAN	PSME3	physical	22939629
PSD12_HUMAN	PSMD12	physical	22939629
PSMD5_HUMAN	PSMD5	physical	22939629
USP9X_HUMAN	USP9X	physical	22939629
SYAP1_HUMAN	SYAP1	physical	22939629
YBOX1_HUMAN	YBX1	physical	22939629
ROA2_HUMAN	HNRNPA2B1	physical	22863883
PSA2_HUMAN	PSMA2	physical	22863883
PSA3_HUMAN	PSMA3	physical	22863883
PSA4_HUMAN	PSMA4	physical	22863883
PSA5_HUMAN	PSMA5	physical	22863883
PSB1_HUMAN	PSMB1	physical	22863883
PSB2_HUMAN	PSMB2	physical	22863883
PSB3_HUMAN	PSMB3	physical	22863883
PSB5_HUMAN	PSMB5	physical	22863883
PSB6_HUMAN	PSMB6	physical	22863883
PSB7_HUMAN	PSMB7	physical	22863883
PSMD9_HUMAN	PSMD9	physical	24720748
PSA5_HUMAN	PSMA5	physical	24720748
ROA1_HUMAN	HNRNPA1	physical	24720748
PRS8_HUMAN	PSMC5	physical	24720748
FSD2_HUMAN	FSD2	physical	25416956
PSB2_HUMAN	PSMB2	physical	26186194
PSD13_HUMAN	PSMD13	physical	26186194
PSA2_HUMAN	PSMA2	physical	26186194
PSA3_HUMAN	PSMA3	physical	26186194
PSB1_HUMAN	PSMB1	physical	26186194
PSA6_HUMAN	PSMA6	physical	26186194
PSME4_HUMAN	PSME4	physical	26186194
PSA7L_HUMAN	PSMA8	physical	26186194
PSA7_HUMAN	PSMA7	physical	26186194
PSD11_HUMAN	PSMD11	physical	26186194
PSME1_HUMAN	PSME1	physical	26186194
PSB7_HUMAN	PSMB7	physical	26186194
PSD12_HUMAN	PSMD12	physical	26186194
FBX7_HUMAN	FBXO7	physical	26186194
POMP_HUMAN	POMP	physical	26186194
PSA5_HUMAN	PSMA5	physical	26186194
PSA4_HUMAN	PSMA4	physical	26186194
PSMG2_HUMAN	PSMG2	physical	26186194
PSB6_HUMAN	PSMB6	physical	26186194
PSB5_HUMAN	PSMB5	physical	26186194
PSB3_HUMAN	PSMB3	physical	26186194
PSME2_HUMAN	PSME2	physical	26186194
PSMF1_HUMAN	PSMF1	physical	26186194
PSB9_HUMAN	PSMB9	physical	26186194
CPSF2_HUMAN	CPSF2	physical	26344197
NELFE_HUMAN	NELFE	physical	26344197
PEX19_HUMAN	PEX19	physical	26344197
PEPL_HUMAN	PPL	physical	26344197
PSA3_HUMAN	PSMA3	physical	26344197
PSA4_HUMAN	PSMA4	physical	26344197
PSB2_HUMAN	PSMB2	physical	26344197
PSB3_HUMAN	PSMB3	physical	26344197
PSB5_HUMAN	PSMB5	physical	26344197
PSB6_HUMAN	PSMB6	physical	26344197
PSB8_HUMAN	PSMB8	physical	26344197
PSB9_HUMAN	PSMB9	physical	26344197
PRS8_HUMAN	PSMC5	physical	26344197
PSD13_HUMAN	PSMD13	physical	26344197
PSMD2_HUMAN	PSMD2	physical	26344197
PSMD6_HUMAN	PSMD6	physical	26344197
U2AF2_HUMAN	U2AF2	physical	26344197
SAE2_HUMAN	UBA2	physical	26344197
PSA7L_HUMAN	PSMA8	physical	28514442
PSB7_HUMAN	PSMB7	physical	28514442
PSME4_HUMAN	PSME4	physical	28514442
PSB9_HUMAN	PSMB9	physical	28514442
PSME2_HUMAN	PSME2	physical	28514442
POMP_HUMAN	POMP	physical	28514442
FBX7_HUMAN	FBXO7	physical	28514442
PSME1_HUMAN	PSME1	physical	28514442
PSMF1_HUMAN	PSMF1	physical	28514442
PSA3_HUMAN	PSMA3	physical	28514442
PSA7_HUMAN	PSMA7	physical	28514442
PSMG2_HUMAN	PSMG2	physical	28514442
PSA2_HUMAN	PSMA2	physical	28514442
PSB5_HUMAN	PSMB5	physical	28514442
PSA6_HUMAN	PSMA6	physical	28514442
PSA4_HUMAN	PSMA4	physical	28514442
PSA5_HUMAN	PSMA5	physical	28514442
PSB6_HUMAN	PSMB6	physical	28514442
PSMG1_HUMAN	PSMG1	physical	28514442
PSD11_HUMAN	PSMD11	physical	28514442
PSMD2_HUMAN	PSMD2	physical	28539385

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PSB4_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Immunoaffinity profiling of tyrosine phosphorylation in cancercells."; Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H.,Zha X.-M., Polakiewicz R.D., Comb M.J.; Nat. Biotechnol. 23:94-101(2005). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-102, AND MASSSPECTROMETRY.	15592455 [Abstract]