dbPTM

SMAD3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SMAD3_HUMAN
UniProt AC	P84022
Protein Name	Mothers against decapentaplegic homolog 3
Gene Name	SMAD3
Organism	Homo sapiens (Human).
Sequence Length	425
Subcellular Localization	Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex
Protein Description	Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator..
Protein Sequence	MSSILPFTPPIVKRLLGWKKGEQNGQEEKWCEKAVKSLVKKLKKTGQLDELEKAITTQNVNTKCITIPRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELRAMELCEFAFNMKKDEVCVNPYHYQRVETPVLPPVLVPRHTEIPAEFPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHASQPSMTVDGFTDPSNSERFCLGLLSNVNRNAAVELTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSIRCSSVS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MSSILPFTP ------CCCCCCCCH	26.53	22814378
2	Phosphorylation	------MSSILPFTP ------CCCCCCCCH	26.53	29255136
3	Phosphorylation	-----MSSILPFTPP -----CCCCCCCCHH	27.18	29255136
8	Phosphorylation	MSSILPFTPPIVKRL CCCCCCCCHHHHHHH	26.64	29255136
13	Ubiquitination	PFTPPIVKRLLGWKK CCCHHHHHHHHCCCC	37.74	-
19	Acetylation	VKRLLGWKKGEQNGQ HHHHHCCCCCCCCCC	49.45	17074756
20	Acetylation	KRLLGWKKGEQNGQE HHHHCCCCCCCCCCH	62.26	17074756
29	Acetylation	EQNGQEEKWCEKAVK CCCCCHHHHHHHHHH	57.29	17478422
33	Acetylation	QEEKWCEKAVKSLVK CHHHHHHHHHHHHHH	56.17	25953088
33	Ubiquitination	QEEKWCEKAVKSLVK CHHHHHHHHHHHHHH	56.17	PubMed
36	Sumoylation	KWCEKAVKSLVKKLK HHHHHHHHHHHHHHH	42.82	-
36	Acetylation	KWCEKAVKSLVKKLK HHHHHHHHHHHHHHH	42.82	25953088
36	Sumoylation	KWCEKAVKSLVKKLK HHHHHHHHHHHHHHH	42.82	-
37	Phosphorylation	WCEKAVKSLVKKLKK HHHHHHHHHHHHHHH	31.62	24719451
45	Phosphorylation	LVKKLKKTGQLDELE HHHHHHHHCCHHHHH	28.68	25599653
53	Ubiquitination	GQLDELEKAITTQNV CCHHHHHHHHHCCCC	58.68	PubMed
63	Ubiquitination	TTQNVNTKCITIPRS HCCCCCCEEEEEECC	20.59	-
66	Phosphorylation	NVNTKCITIPRSLDG CCCCEEEEEECCCCC	34.11	24719451
78	Phosphorylation	LDGRLQVSHRKGLPH CCCCEEEECCCCCCE	12.47	26670566
81	Ubiquitination	RLQVSHRKGLPHVIY CEEEECCCCCCEEEE	60.42	PubMed
88	Phosphorylation	KGLPHVIYCRLWRWP CCCCEEEEEEECCCC	3.14	-
125	Phosphorylation	DEVCVNPYHYQRVET CCEEECCCCCCCCCC	14.14	-
132	Phosphorylation	YHYQRVETPVLPPVL CCCCCCCCCCCCCCC	18.81	30266825
179	Phosphorylation	PQSNIPETPPPGYLS CCCCCCCCCCCCCCC	35.42	19218245
183	Acetylation	IPETPPPGYLSEDGE CCCCCCCCCCCCCCC	41.61	19608861
204	Phosphorylation	NHSMDAGSPNLSPNP CCCCCCCCCCCCCCC	16.61	19218245
208	Phosphorylation	DAGSPNLSPNPMSPA CCCCCCCCCCCCCCC	29.30	14512875
213	Phosphorylation	NLSPNPMSPAHNNLD CCCCCCCCCCCCCCC	21.86	18032789
264	Phosphorylation	VDGFTDPSNSERFCL ECCCCCCCCCHHHHH	56.66	-
273	Acetylation	SERFCLGLLSNVNRN CHHHHHHHHHCCCHH	2.82	19608861
275	Phosphorylation	RFCLGLLSNVNRNAA HHHHHHHHCCCHHHH	43.72	21712546
286	Phosphorylation	RNAAVELTRRHIGRG HHHHHHHHHHHCCCC	16.49	29262532
309	Phosphorylation	EVFAECLSDSAIFVQ HHHHHHHCCCEEEEE	40.97	-
333	Acetylation	WHPATVCKIPPGCNL CCCCCEEECCCCCCE	54.64	25953088
334	Acetylation	HPATVCKIPPGCNLK CCCCEEECCCCCCEE	4.15	19608861
375	Phosphorylation	RMCTIRMSFVKGWGA HHHHHHHHHHCCCCC	19.62	22393057
378	Malonylation	TIRMSFVKGWGAEYR HHHHHHHCCCCCCHH	47.11	26320211
378	Acetylation	TIRMSFVKGWGAEYR HHHHHHHCCCCCCHH	47.11	19608861
388	Phosphorylation	GAEYRRQTVTSTPCW CCCHHCEECCCCCEE	24.76	-
412	Phosphorylation	QWLDKVLTQMGSPSI HHHHHHHHHCCCCCC	21.35	29396449
416	Phosphorylation	KVLTQMGSPSIRCSS HHHHHCCCCCCCCCC	15.33	25159151
418	Phosphorylation	LTQMGSPSIRCSSVS HHHCCCCCCCCCCCC	25.10	21712546
422	Phosphorylation	GSPSIRCSSVS---- CCCCCCCCCCC----	24.75	25056879
423	Phosphorylation	SPSIRCSSVS----- CCCCCCCCCC-----	29.97	9380693
425	Phosphorylation	SIRCSSVS------- CCCCCCCC-------	36.13	9380693

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
8	T	Phosphorylation	Kinase	CDK2	P24941	Uniprot
8	T	Phosphorylation	Kinase	CDK4	P30285	PSP
8	T	Phosphorylation	Kinase	CDK2	P97377	PSP
8	T	Phosphorylation	Kinase	CDK4	P11802	Uniprot
179	T	Phosphorylation	Kinase	CDK9	P50750	PSP
179	T	Phosphorylation	Kinase	CDK8	P49336	PSP
179	T	Phosphorylation	Kinase	CDK4	P30285	PSP
179	T	Phosphorylation	Kinase	MAPK1	P28482	GPS
179	T	Phosphorylation	Kinase	MAPK	-	Uniprot
179	T	Phosphorylation	Kinase	CDK4	P11802	Uniprot
179	T	Phosphorylation	Kinase	CDK2	P97377	PSP
179	T	Phosphorylation	Kinase	AKT1	P31749	PSP
179	T	Phosphorylation	Kinase	CDK2	P24941	Uniprot
204	S	Phosphorylation	Kinase	MAPK	-	Uniprot
204	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
204	S	Phosphorylation	Kinase	MELK	Q61846	PSP
204	S	Phosphorylation	Kinase	GSK3	-	Uniprot
204	S	Phosphorylation	Kinase	CDK4	P11802	PSP
208	S	Phosphorylation	Kinase	MAPK	-	Uniprot
208	S	Phosphorylation	Kinase	CDK9	P50750	PSP
208	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
208	S	Phosphorylation	Kinase	CDK4	P11802	PSP
208	S	Phosphorylation	Kinase	CDK8	P49336	PSP
213	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
213	S	Phosphorylation	Kinase	CDK2	P24941	Uniprot
213	S	Phosphorylation	Kinase	CDK2	P97377	PSP
213	S	Phosphorylation	Kinase	CDK4	P11802	Uniprot
213	S	Phosphorylation	Kinase	CDK4	P30285	PSP
213	S	Phosphorylation	Kinase	CDK9	P50750	PSP
213	S	Phosphorylation	Kinase	CDK8	P49336	PSP
418	S	Phosphorylation	Kinase	CK1G2	P78368	PSP
418	S	Phosphorylation	Kinase	CK1	-	Uniprot
422	S	Phosphorylation	Kinase	TGFBR1	P36897	Uniprot
423	S	Phosphorylation	Kinase	TGFBR1	P36897	Uniprot
425	S	Phosphorylation	Kinase	TGFBR1	P36897	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	WWP2	O00308	PMID:21258410
-	K	Ubiquitination	E3 ubiquitin ligase	NEDD4L	Q96PU5	PMID:19917253
-	K	Ubiquitination	E3 ubiquitin ligase	STUB1	Q9UNE7	PMID:15781469
-	K	Ubiquitination	E3 ubiquitin ligase	BTRC	Q9Y297	PMID:11359933
-	K	Ubiquitination	E3 ubiquitin ligase	PJA1	Q8NG27	PMID:22199232
-	K	Ubiquitination	E3 ubiquitin ligase	RNF111	Q6ZNA4	PMID:18950738
-	K	Ubiquitination	E3 ubiquitin ligase	SMURF2	Q9HAU4	PMID:15108358

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
179	T	Phosphorylation	15241418
Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment.
204	S	Phosphorylation	15241418
Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment.
208	S	Phosphorylation	15241418
Enhanced phosphorylation in the linker region on Thr-179, Ser-204 and Ser-208 on EGF and TGF-beta treatment.
208	S	Phosphorylation	15241418
Ser-208 is the main site of MAPK-mediated phosphorylation.
418	S	Phosphorylation	18794808
Phosphorylation at Ser-418 by CSNK1G2/CK1 promotes ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses.
418	S	ubiquitylation	18794808
Phosphorylation at Ser-418 by CSNK1G2/CK1 promotes ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SMAD3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RRAS2_MOUSE	Rras2	physical	15761153
ARHG7_MOUSE	Arhgef7	physical	15761153
2ABD_MOUSE	Ppp2r2d	physical	15761153
WWP2_MOUSE	Wwp2	physical	15761153
RHES_MOUSE	Rasd2	physical	15761153
RAB38_MOUSE	Rab38	physical	15761153
FBX3_MOUSE	Fbxo3	physical	15761153
SQSTM_MOUSE	Sqstm1	physical	15761153
MP2K3_MOUSE	Map2k3	physical	15761153
WEE1_MOUSE	Wee1	physical	15761153
ARHG6_MOUSE	Arhgef6	physical	15761153
RGS3_MOUSE	Rgs3	physical	15761153
TRI35_MOUSE	Trim35	physical	15761153
CLASR_MOUSE	Clasrp	physical	15761153
SMAD2_MOUSE	Smad2	physical	15761153
SMAD3_MOUSE	Smad3	physical	15761153
GSC_MOUSE	Gsc	physical	15761153
ANDR_HUMAN	AR	physical	11280774
ANDR_HUMAN	AR	physical	11707452
PML_HUMAN	PML	physical	15356634
ZFYV9_HUMAN	ZFYVE9	physical	15356634
APC10_HUMAN	ANAPC10	physical	15144564
CASL_HUMAN	NEDD9	physical	15144564
FOXO1_HUMAN	FOXO1	physical	15084259
FOXO4_HUMAN	FOXO4	physical	15084259
FOXO3_HUMAN	FOXO3	physical	15084259
SMAD4_HUMAN	SMAD4	physical	15084259
FBW1A_HUMAN	BTRC	physical	14988407
PIAS3_HUMAN	PIAS3	physical	14691252
NOTC1_HUMAN	NOTCH1	physical	14638857
EID2_HUMAN	EID2	physical	14612439
GCR_HUMAN	NR3C1	physical	12902338
SKI_HUMAN	SKI	physical	12874272
HIPK2_HUMAN	HIPK2	physical	12874272
SKI_HUMAN	SKI	physical	12857746
ATF3_HUMAN	ATF3	physical	12718878
DVL1_HUMAN	DVL1	physical	12650946
ERBIN_HUMAN	ERBB2IP	physical	12650946
ATF2_HUMAN	ATF2	physical	10085140
SKIL_HUMAN	SKIL	physical	11691834
CDC27_HUMAN	CDC27	physical	11691834
CDC16_HUMAN	CDC16	physical	11691834
DAB2_HUMAN	DAB2	physical	11387212
JUN_HUMAN	JUN	physical	9732876
FOS_HUMAN	FOS	physical	9732876
SMAD4_HUMAN	SMAD4	physical	12419246
IMB1_HUMAN	KPNB1	physical	10846168
RBL1_HUMAN	RBL1	physical	12150994
E2F4_HUMAN	E2F4	physical	12150994
JUN_HUMAN	JUN	physical	10903323
CTNB1_HUMAN	CTNNB1	physical	12000714
SKIL_HUMAN	SKIL	physical	10531062
RUNX2_HUMAN	RUNX2	physical	10962029
TFE3_HUMAN	TFE3	physical	12551947
MAX_HUMAN	MAX	physical	12551947
SMUF2_HUMAN	SMURF2	physical	11016919
AKT1_HUMAN	AKT1	physical	16362038
SMAD2_HUMAN	SMAD2	physical	9311995
SMAD4_HUMAN	SMAD4	physical	9311995
MEN1_HUMAN	MEN1	physical	11274402
NFYC_HUMAN	NFYC	physical	12023901
ANDR_HUMAN	AR	physical	12226080
JUNB_HUMAN	JUNB	physical	10220381
JUN_HUMAN	JUN	physical	10220381
JUND_HUMAN	JUND	physical	10220381
RUNX1_HUMAN	RUNX1	physical	10531362
RUNX2_HUMAN	RUNX2	physical	10531362
RUNX3_HUMAN	RUNX3	physical	10531362
SNW1_HUMAN	SNW1	physical	11278756
STRAP_HUMAN	STRAP	physical	10757800
SMAD4_HUMAN	SMAD4	physical	9892009
SMAD7_HUMAN	SMAD7	genetic	9892009
CASL_HUMAN	NEDD9	physical	11118211
SMAD4_HUMAN	SMAD4	physical	11114293
SP1_HUMAN	SP1	physical	11114293
CEBPA_HUMAN	CEBPA	physical	12524424
CEBPB_HUMAN	CEBPB	physical	12524424
CEBPD_HUMAN	CEBPD	physical	12524424
PIN1_HUMAN	PIN1	physical	19122240
RN111_HUMAN	RNF111	physical	18950738
KC1G2_HUMAN	CSNK1G2	physical	18794808
GSK3B_HUMAN	GSK3B	physical	18172167
KDM5C_HUMAN	KDM5C	physical	18078810
SMAD4_HUMAN	SMAD4	physical	20142324
BMP7_HUMAN	BMP7	physical	20093492
UCHL5_HUMAN	UCHL5	physical	16027725
BPTF_HUMAN	BPTF	physical	18974875
MPIP1_HUMAN	CDC25A	physical	15798217
CUL1_HUMAN	CUL1	physical	15798217
CREST_HUMAN	SS18L1	physical	20211142
ZBTB3_HUMAN	ZBTB3	physical	20211142
NEDD4_HUMAN	NEDD4	physical	15496141
CITE2_HUMAN	CITED2	physical	16619037
WWP1_HUMAN	WWP1	physical	15221015
SMUF1_HUMAN	SMURF1	physical	15221015
SMUF2_HUMAN	SMURF2	physical	15221015
GRIP1_HUMAN	GRIP1	physical	16423881
PIAS4_HUMAN	PIAS4	physical	12904571
MYCD_HUMAN	MYOCD	physical	16224064
TYY1_HUMAN	YY1	physical	12808092
SKI_HUMAN	SKI	physical	12732634
SMAD4_HUMAN	SMAD4	physical	12732634
MED15_HUMAN	MED15	physical	12167862
MED24_HUMAN	MED24	physical	12167862
MED6_HUMAN	MED6	physical	12167862
SMAD4_HUMAN	SMAD4	physical	12167862
KAT2B_HUMAN	KAT2B	physical	15994459
HDAC5_HUMAN	HDAC5	physical	15990875
HDAC4_HUMAN	HDAC4	physical	15990875
EVI1_HUMAN	MECOM	physical	15849193
CBP_HUMAN	CREBBP	physical	15849193
BRCA1_HUMAN	BRCA1	physical	15735739
SOX9_HUMAN	SOX9	physical	15623506
PITX1_HUMAN	PITX1	physical	18339718
PITX2_HUMAN	PITX2	physical	18339718
HDAC1_HUMAN	HDAC1	physical	11306568
SKI_HUMAN	SKI	physical	11306568
SKIL_HUMAN	SKIL	physical	11306568
TGIF1_HUMAN	TGIF1	physical	11306568
SIN3A_HUMAN	SIN3A	physical	11306568
JUN_HUMAN	JUN	physical	11306568
KAT2B_HUMAN	KAT2B	physical	11058129
EP300_HUMAN	EP300	physical	11058129
EP300_HUMAN	EP300	physical	10575014
SKI_HUMAN	SKI	physical	10575014
HDAC1_HUMAN	HDAC1	physical	10575014
SIN3A_MOUSE	Sin3a	physical	10575014
EP300_HUMAN	EP300	physical	15133024
VDR_HUMAN	VDR	physical	10037600
KAT2A_HUMAN	KAT2A	physical	15009097
KAT2B_HUMAN	KAT2B	physical	15009097
CBP_HUMAN	CREBBP	physical	18003620
SMRC2_HUMAN	SMARCC2	physical	18003620
ARI1A_HUMAN	ARID1A	physical	18003620
SMRC1_HUMAN	SMARCC1	physical	18003620
SMCA4_HUMAN	SMARCA4	physical	18003620
ERBIN_HUMAN	ERBB2IP	physical	18003620
TRI33_HUMAN	TRIM33	physical	18003620
NCOA3_HUMAN	NCOA3	physical	18003620
RBX1_HUMAN	RBX1	physical	11359933
SMUF2_HUMAN	SMURF2	physical	11158580
CTCF_HUMAN	CTCF	physical	20427289
CTNB1_HUMAN	CTNNB1	physical	20571128
SMAD4_HUMAN	SMAD4	physical	20564330
EP300_HUMAN	EP300	physical	20564330
ATF3_HUMAN	ATF3	physical	20930144
ESR1_HUMAN	ESR1	physical	20207742
SRY_HUMAN	SRY	physical	15527767
ACTB_HUMAN	ACTB	physical	15527767
PRTN3_HUMAN	PRTN3	physical	15527767
SRBP2_HUMAN	SREBF2	physical	15527767
SMAD4_HUMAN	SMAD4	physical	22045334
CHIP_HUMAN	STUB1	physical	14701756
WWP2_HUMAN	WWP2	physical	21258410
CBP_HUMAN	CREBBP	physical	15750622
FOXH1_HUMAN	FOXH1	physical	15750622
LEF1_HUMAN	LEF1	physical	15750622
CTNB1_HUMAN	CTNNB1	physical	21965288
USP9X_HUMAN	USP9X	physical	19135894
SMAD4_HUMAN	SMAD4	physical	19135894
EP300_HUMAN	EP300	physical	9679056
SP1_HUMAN	SP1	physical	10878024
HNF4A_HUMAN	HNF4A	physical	10995777
SKI_HUMAN	SKI	physical	12426322
SKIL_HUMAN	SKIL	physical	12426322
NU214_HUMAN	NUP214	physical	12917407
NU153_HUMAN	NUP153	physical	12917407
SMAD4_HUMAN	SMAD4	physical	12917407
UBP15_HUMAN	USP15	physical	21947082
CTNB1_HUMAN	CTNNB1	physical	22241478
YAP1_HUMAN	YAP1	physical	19914168
SKI_HUMAN	SKI	physical	17283070
CBP_HUMAN	CREBBP	physical	17283070
SMAD4_HUMAN	SMAD4	physical	17283070
EPAS1_HUMAN	EPAS1	physical	21057546
RGCC_HUMAN	RGCC	physical	21990365
SUV91_HUMAN	SUV39H1	physical	21862595
CBL_HUMAN	CBL	physical	22310290
SMAD4_HUMAN	SMAD4	physical	22310290
ZEB2_HUMAN	ZEB2	physical	16061479
PIN1_HUMAN	PIN1	physical	21685363
NEDD4_HUMAN	NEDD4	physical	21685363
NED4L_HUMAN	NEDD4L	physical	19917253
SMAD4_HUMAN	SMAD4	physical	19917253
TRIB3_HUMAN	TRIB3	physical	21896644
UBP15_HUMAN	USP15	physical	22344298
HGS_HUMAN	HGS	physical	11094085
NED4L_HUMAN	NEDD4L	physical	22921829
PAX6_HUMAN	PAX6	physical	17251190
BRCA1_HUMAN	BRCA1	physical	19768112
SMAD4_HUMAN	SMAD4	physical	19768112
ZMIZ1_HUMAN	ZMIZ1	physical	16777850
SMAD4_HUMAN	SMAD4	physical	18729074
SMAD2_HUMAN	SMAD2	physical	18729074
KC1D_HUMAN	CSNK1D	physical	18729074
DDX3Y_HUMAN	DDX3Y	physical	18729074
ERBIN_HUMAN	ERBB2IP	physical	18729074
MK01_HUMAN	MAPK1	physical	18729074
HSP74_HUMAN	HSPA4	physical	18729074
MAN1_HUMAN	LEMD3	physical	18729074
PJA1_HUMAN	PJA1	physical	18729074
ZFYV9_HUMAN	ZFYVE9	physical	18729074
SNW1_HUMAN	SNW1	physical	18729074
SMUF2_HUMAN	SMURF2	physical	18729074
WWP2_HUMAN	WWP2	physical	18729074
TYY1_HUMAN	YY1	physical	18729074
RL3_HUMAN	RPL3	physical	18729074
ATX2L_HUMAN	ATXN2L	physical	18729074
FA83G_HUMAN	FAM83G	physical	18729074
CHD8_HUMAN	CHD8	physical	18729074
CMC1_HUMAN	SLC25A12	physical	18729074
NHRF2_HUMAN	SLC9A3R2	physical	18729074
LRCH1_HUMAN	LRCH1	physical	18729074
YTHD2_HUMAN	YTHDF2	physical	18729074
TCPA_HUMAN	TCP1	physical	18729074
E41L5_HUMAN	EPB41L5	physical	18729074
PTN6_HUMAN	PTPN6	physical	18729074
DOCK9_HUMAN	DOCK9	physical	18729074
PSPC1_HUMAN	PSPC1	physical	18729074
DDX4_HUMAN	DDX4	physical	18729074
DDX3X_HUMAN	DDX3X	physical	18729074
DNJA2_HUMAN	DNAJA2	physical	18729074
SIK3_HUMAN	SIK3	physical	18729074
U2AF2_HUMAN	U2AF2	physical	18729074
DHX8_HUMAN	DHX8	physical	18729074
SARG_HUMAN	C1orf116	physical	18729074
ITB4_HUMAN	ITGB4	physical	18729074
CD11A_HUMAN	CDK11A	physical	18729074
RFIP5_HUMAN	RAB11FIP5	physical	18729074
SMAD1_HUMAN	SMAD1	physical	18729074
IRS1_HUMAN	IRS1	physical	18729074
SMAD5_HUMAN	SMAD5	physical	18729074
DYR1A_HUMAN	DYRK1A	physical	18729074
BUB3_HUMAN	BUB3	physical	18729074
SQOR_HUMAN	SQRDL	physical	18729074
PABP1_HUMAN	PABPC1	physical	18729074
DCAF7_HUMAN	DCAF7	physical	18729074
OPA1_HUMAN	OPA1	physical	18729074
VAV2_HUMAN	VAV2	physical	18729074
UBP7_HUMAN	USP7	physical	18729074
RL4_HUMAN	RPL4	physical	18729074
TGFR1_HUMAN	TGFBR1	physical	19758997
SP1_HUMAN	SP1	physical	16714330
SMAD4_HUMAN	SMAD4	physical	15922743
S10A4_HUMAN	S100A4	physical	20070253
ERBIN_HUMAN	ERBB2IP	physical	17591701
SMAD4_HUMAN	SMAD4	physical	17591701
IKKA_HUMAN	CHUK	physical	18268325
A4_HUMAN	APP	physical	21832049
SMAD4_HUMAN	SMAD4	physical	15350224
SMAD4_HUMAN	SMAD4	physical	10092624
SMAD3_HUMAN	SMAD3	physical	10092624
PIN1_HUMAN	PIN1	physical	19920136
HIF1A_HUMAN	HIF1A	physical	11486006
RNZ2_HUMAN	ELAC2	physical	16636667
NOTC4_HUMAN	NOTCH4	physical	16007227
SMAD1_HUMAN	SMAD1	physical	9111321
SMAD2_HUMAN	SMAD2	physical	9111321
SMAD3_HUMAN	SMAD3	physical	9111321
SMAD4_HUMAN	SMAD4	physical	9111321
SMAD4_HUMAN	SMAD4	physical	15467747
PSD11_HUMAN	PSMD11	physical	15231748
ZEB2_HUMAN	ZEB2	physical	15231748
GANP_HUMAN	MCM3AP	physical	15231748
ZFYV9_HUMAN	ZFYVE9	physical	15231748
CP11A_HUMAN	CYP11A1	physical	15231748
RLA0_HUMAN	RPLP0	physical	15231748
RU17_HUMAN	SNRNP70	physical	15231748
XRCC6_HUMAN	XRCC6	physical	15231748
ZEP1_HUMAN	HIVEP1	physical	15231748
FLNA_HUMAN	FLNA	physical	15231748
TGM2_HUMAN	TGM2	physical	15231748
2AAA_HUMAN	PPP2R1A	physical	15231748
ZNF83_HUMAN	ZNF83	physical	15231748
ZBT16_HUMAN	ZBTB16	physical	15231748
PCDH1_HUMAN	PCDH1	physical	15231748
PAPP1_HUMAN	PAPPA	physical	15231748
TMED1_HUMAN	TMED1	physical	15231748
ANK3_HUMAN	ANK3	physical	15231748
SMAD4_HUMAN	SMAD4	physical	15231748
IL27B_HUMAN	EBI3	physical	15231748
NCOA6_HUMAN	NCOA6	physical	15231748
PLAG1_HUMAN	PLAG1	physical	15231748
CXXC5_HUMAN	CXXC5	physical	15231748
KANL1_HUMAN	KANSL1	physical	15231748
ZN329_HUMAN	ZNF329	physical	15231748
GGA1_HUMAN	GGA1	physical	15231748
SVEP1_HUMAN	SVEP1	physical	15231748
RANB9_HUMAN	RANBP9	physical	15231748
EPAS1_HUMAN	EPAS1	physical	15231748
SP130_HUMAN	SAP130	physical	15231748
VISTA_HUMAN	C10orf54	physical	15231748
SMUF1_HUMAN	SMURF1	physical	15231748
HEYL_HUMAN	HEYL	physical	15231748
CFDP1_HUMAN	CFDP1	physical	15231748
SIA7B_HUMAN	ST6GALNAC2	physical	15231748
PRDM4_HUMAN	PRDM4	physical	15231748
PLAL1_HUMAN	PLAGL1	physical	15231748
KMT2B_HUMAN	KMT2B	physical	15231748
KDM2A_HUMAN	KDM2A	physical	15231748
MTF2_HUMAN	MTF2	physical	15231748
HEY1_HUMAN	HEY1	physical	15231748
GMEB1_HUMAN	GMEB1	physical	15231748
TGFR1_HUMAN	TGFBR1	physical	12543979
SMAD2_HUMAN	SMAD2	physical	12543979
SMAD4_HUMAN	SMAD4	physical	12543979
SPTB2_HUMAN	SPTBN1	physical	12543979
SMAD3_HUMAN	SMAD3	physical	12154125
ZFYV9_HUMAN	ZFYVE9	physical	12154125
SKI_HUMAN	SKI	physical	12154125
DEDD_HUMAN	DEDD	physical	20553715
SH22A_HUMAN	SH2D2A	physical	16806069
IRF7_HUMAN	IRF7	physical	14729983
TSC2_HUMAN	TSC2	physical	15066998
FHL1_HUMAN	FHL1	physical	19139564
RUNX1_HUMAN	RUNX1	physical	15897867
TGFI1_HUMAN	TGFB1I1	physical	15561701
SMAD4_HUMAN	SMAD4	physical	23788427
SQSTM_HUMAN	SQSTM1	physical	21988832
ZC12A_HUMAN	ZC3H12A	physical	21988832
T22D4_HUMAN	TSC22D4	physical	21988832
ZMY11_HUMAN	ZMYND11	physical	21988832
UBQL4_HUMAN	UBQLN4	physical	21988832
TYDP2_HUMAN	TDP2	physical	21988832
TRFE_HUMAN	TF	physical	21988832
SMAD4_HUMAN	SMAD4	physical	21988832
TLR4_HUMAN	TLR4	physical	21988832
UBP7_HUMAN	USP7	physical	21988832
TANK_HUMAN	TANK	physical	21988832
ZCH14_HUMAN	ZCCHC14	physical	21988832
P63_HUMAN	TP63	physical	23687300
TRI62_HUMAN	TRIM62	physical	23838884
SMUF2_HUMAN	SMURF2	physical	23973329
FOXM1_HUMAN	FOXM1	physical	24382352
SMAD4_HUMAN	SMAD4	physical	24382352
TRI33_HUMAN	TRIM33	physical	24382352
FOXO3_HUMAN	FOXO3	physical	24920680
MAFK_HUMAN	MAFK	physical	23737527
BACH1_HUMAN	BACH1	physical	23737527
SMAD4_HUMAN	SMAD4	physical	24157709
MEOX2_HUMAN	MEOX2	physical	25416956
GO45_HUMAN	BLZF1	physical	25416956
WWP1_HUMAN	WWP1	physical	25416956
CPSF7_HUMAN	CPSF7	physical	25416956
CCD33_HUMAN	CCDC33	physical	25416956
TEKT4_HUMAN	TEKT4	physical	25416956
EP300_HUMAN	EP300	physical	16109717
EP300_HUMAN	EP300	physical	12743039
CBP_HUMAN	CREBBP	physical	19589780
EP300_HUMAN	EP300	physical	21567395
EP300_HUMAN	EP300	physical	17469184
SKI_HUMAN	SKI	physical	17469184
SKIL_HUMAN	SKIL	physical	17469184
DDX5_HUMAN	DDX5	physical	19917253
IF4B_HUMAN	EIF4B	physical	19917253
MAN1_HUMAN	LEMD3	physical	19917253
XPO6_HUMAN	XPO6	physical	19917253
TRI33_HUMAN	TRIM33	physical	19917253
DOK1_MOUSE	Dok1	physical	11927552
SMAD4_HUMAN	SMAD4	physical	11927552
ZFYV9_HUMAN	ZFYVE9	physical	26186194
MAN1_HUMAN	LEMD3	physical	26186194
SMAD9_HUMAN	SMAD9	physical	26186194
SMAD2_HUMAN	SMAD2	physical	26186194
LACRT_HUMAN	LACRT	physical	26186194
STRAP_HUMAN	STRAP	genetic	10757800
NFIA_HUMAN	NFIA	physical	18215124
NCOA1_HUMAN	NCOA1	physical	16423881
ERBIN_MOUSE	Erbb2ip	physical	12650946
PARD3_MOUSE	Pard3	physical	12650946
DVL1_MOUSE	Dvl1	physical	12650946
PARD3_HUMAN	PARD3	physical	12650946
SMAD4_HUMAN	SMAD4	physical	24584437
KAT2B_HUMAN	KAT2B	physical	22995475
SMAD4_HUMAN	SMAD4	physical	25514493
SMAD7_HUMAN	SMAD7	physical	26555259
SMAD4_HUMAN	SMAD4	physical	26555259
NED4L_HUMAN	NEDD4L	physical	26555259
UCHL5_HUMAN	UCHL5	physical	27604640
SMAD4_HUMAN	SMAD4	physical	28115363
SMAD2_HUMAN	SMAD2	physical	28514442
SMAD9_HUMAN	SMAD9	physical	28514442
ZFYV9_HUMAN	ZFYVE9	physical	28514442
MAN1_HUMAN	LEMD3	physical	28514442
LACRT_HUMAN	LACRT	physical	28514442
SMUF1_HUMAN	SMURF1	physical	28839186
SMUF2_HUMAN	SMURF2	physical	28881580

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
114500	Colorectal cancer (CRC)
613795	Loeys-Dietz syndrome 3 (LDS3)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SMAD3_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-378, AND MASS SPECTROMETRY.	19608861 [Abstract]
"Smad3 is acetylated by p300/CBP to regulate its transactivationactivity."; Inoue Y., Itoh Y., Abe K., Okamoto T., Daitoku H., Fukamizu A.,Onozaki K., Hayashi H.; Oncogene 26:500-508(2007). Cited for: ACETYLATION AT LYS-378, FUNCTION, AND MUTAGENESIS OF LYS-333; LYS-341;LYS-378; LYS-409 AND 422-SER--SER-425.	16862174 [Abstract]
Phosphorylation
Reference	PubMed
"Transforming growth factor-{beta}-inducible phosphorylation ofSmad3."; Wang G., Matsuura I., He D., Liu F.; J. Biol. Chem. 284:9663-9673(2009). Cited for: PHOSPHORYLATION AT THR-179; SER-204 AND SER-208, SUBCELLULAR LOCATION,FUNCTION, AND MUTAGENESIS OF THR-179; SER-204 AND SER-208.	19218245 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-416, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Ligand-dependent ubiquitination of Smad3 is regulated by caseinkinase 1 gamma 2, an inhibitor of TGF-beta signaling."; Guo X., Waddell D.S., Wang W., Wang Z., Liberati N.T., Yong S.,Liu X., Wang X.-F.; Oncogene 27:7235-7247(2008). Cited for: INTERACTION WITH CSNK1G2, UBIQUITINATION, PHOSPHORYLATION AT SER-418BY CSNK1G2/CK1, AND MUTAGENESIS OF SER-418.	18794808 [Abstract]
"Identification and characterization of ERK MAP kinase phosphorylationsites in Smad3."; Matsuura I., Wang G., He D., Liu F.; Biochemistry 44:12546-12553(2005). Cited for: PHOSPHORYLATION AT THR-179; SER-204 AND SER-208, SUBCELLULAR LOCATION,FUNCTION, AND MUTAGENESIS OF THR-179; SER-204 AND SER-208.	16156666 [Abstract]
"Cyclin-dependent kinases regulate the antiproliferative function ofSmads."; Matsuura I., Denissova N.G., Wang G., He D., Long J., Liu F.; Nature 430:226-231(2004). Cited for: PHOSPHORYLATION AT THR-8; THR-179; SER-204; SER-208 AND SER-213,FUNCTION, AND MUTAGENESIS OF THR-8; THR-179; SER-204; SER-208 ANDSER-213.	15241418 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-8, AND MASSSPECTROMETRY.	19413330 [Abstract]

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