dbPTM

PARD3_MOUSE - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PARD3_MOUSE
UniProt AC	Q99NH2
Protein Name	Partitioning defective 3 homolog
Gene Name	Pard3
Organism	Mus musculus (Mouse).
Sequence Length	1333
Subcellular Localization	Cytoplasm. Endomembrane system. Cell junction. Cell junction, tight junction . Cell junction, adherens junction . Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell membrane. Localized along the cell-cell contact region. Colocalizes with PARD6A an
Protein Description	Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. [PubMed: 11839275 The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity Involved in Schwann cell peripheral myelination]
Protein Sequence	MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASFTGTQSPEIFGSELGTNNVSAFQPYQATSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTAGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREKNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPAAPALAPPSVLSTNVGSVYNTKKVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQTPKETKAEDEDVVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSDASQLDFVKTRKSKSMDLVADETKLNTVDDQRAGSPSRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKTERKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYEGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSISQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Phosphorylation	DGRMKVFSLIQQAVT CCHHHHHHHHHHHHH	27.79	26643407
44	Phosphorylation	AVAKDPNYWIQVHRL HHHCCCCEEEEEEEC	14.85	29514104
91	Phosphorylation	PHHGGDGTSASFTGT CCCCCCCCCCCCCCC	26.34	-
143	Phosphorylation	MPLHVRRSSDPALTG CCEEEECCCCCCHHC	28.64	25521595
144	Phosphorylation	PLHVRRSSDPALTGL CEEEECCCCCCHHCC	45.89	25521595
149	Phosphorylation	RSSDPALTGLSTSVS CCCCCCHHCCCCCCC	37.88	27087446
152	Phosphorylation	DPALTGLSTSVSDNN CCCHHCCCCCCCCCC	21.71	25619855
153	Phosphorylation	PALTGLSTSVSDNNF CCHHCCCCCCCCCCC	37.88	25619855
154	Phosphorylation	ALTGLSTSVSDNNFS CHHCCCCCCCCCCCC	19.09	25619855
156	Phosphorylation	TGLSTSVSDNNFSSE HCCCCCCCCCCCCCC	34.09	25521595
161	Phosphorylation	SVSDNNFSSEEPSRK CCCCCCCCCCCCCCC	38.68	25619855
162	Phosphorylation	VSDNNFSSEEPSRKN CCCCCCCCCCCCCCC	41.67	25619855
166	Phosphorylation	NFSSEEPSRKNPTRW CCCCCCCCCCCCCCC	60.20	25619855
171	Phosphorylation	EPSRKNPTRWSTTAG CCCCCCCCCCCCHHH	54.91	22324799
174	Phosphorylation	RKNPTRWSTTAGFLK CCCCCCCCCHHHHHH	16.76	26824392
175	Phosphorylation	KNPTRWSTTAGFLKQ CCCCCCCCHHHHHHH	17.44	26160508
176	Phosphorylation	NPTRWSTTAGFLKQN CCCCCCCHHHHHHHC	21.19	26239621
181	Ubiquitination	STTAGFLKQNTAGSP CCHHHHHHHCCCCCC	38.96	-
187	Phosphorylation	LKQNTAGSPKTCDRK HHHCCCCCCCCCCCC	22.28	22324799
199	Phosphorylation	DRKKDENYRSLPRDP CCCCCCCCCCCCCCC	10.26	29550500
201	Phosphorylation	KKDENYRSLPRDPSS CCCCCCCCCCCCCCH	32.16	26824392
207	Phosphorylation	RSLPRDPSSWSNQFQ CCCCCCCCHHHHHHH	49.05	26160508
208	Phosphorylation	SLPRDPSSWSNQFQR CCCCCCCHHHHHHHH	39.65	26160508
210	Phosphorylation	PRDPSSWSNQFQRDN CCCCCHHHHHHHHHC	23.77	26643407
220	Phosphorylation	FQRDNARSSLSASHP HHHHCHHHHHHHCCH	32.97	22942356
221	Phosphorylation	QRDNARSSLSASHPM HHHCHHHHHHHCCHH	22.31	25521595
223	Phosphorylation	DNARSSLSASHPMVD HCHHHHHHHCCHHHH	29.67	25168779
225	Phosphorylation	ARSSLSASHPMVDRW HHHHHHHCCHHHHHH	25.42	26239621
235	Ubiquitination	MVDRWLEKQEQDEEG HHHHHHHHHHHCCCC	57.89	-
377	Phosphorylation	SQREKNNYSPGRFSP HHHHHHCCCCCCCCC	26.15	26160508
378	Phosphorylation	QREKNNYSPGRFSPD HHHHHCCCCCCCCCC	24.01	27087446
383	Phosphorylation	NYSPGRFSPDSHCVA CCCCCCCCCCCCCCC	26.83	27087446
386	Phosphorylation	PGRFSPDSHCVANRS CCCCCCCCCCCCCHH	23.36	26643407
393	Phosphorylation	SHCVANRSVANNAPQ CCCCCCHHHHCCCCC	25.95	28066266
449	Phosphorylation	VLSTNVGSVYNTKKV HHCCCCCCEECCCCH	19.69	29514104
474	Phosphorylation	GTEGLGFSITSRDVT CCCCCCEEEEECCEE	24.14	25338131
481	Phosphorylation	SITSRDVTIGGSAPI EEEECCEEECCCCCE	20.42	28066266
485	Phosphorylation	RDVTIGGSAPIYVKN CCEEECCCCCEEEEE	26.17	28066266
489	Phosphorylation	IGGSAPIYVKNILPR ECCCCCEEEEECCCC	12.33	22499769
567	Ubiquitination	PSQMQTPKETKAEDE HHHCCCCCCCCCCCC	79.84	-
579	Phosphorylation	EDEDVVLTPDGTREF CCCCEEECCCCCCEE	14.02	25521595
583	Phosphorylation	VVLTPDGTREFLTFE EEECCCCCCEEEEEE	34.11	25619855
611	Ubiquitination	SVKGNRSKENHADLG EEECCCCCCCCCCCE	60.34	-
622	Acetylation	ADLGIFVKSIINGGA CCCEEEEHHHHCCCC	25.78	15613441
632	Ubiquitination	INGGAASKDGRLRVN HCCCCCCCCCCCEEC	60.52	-
661	Phosphorylation	ANQEAMETLRRSMST HHHHHHHHHHHHHCC	16.69	26643407
665	Phosphorylation	AMETLRRSMSTEGNK HHHHHHHHHCCCCCH	15.62	26643407
667	Phosphorylation	ETLRRSMSTEGNKRG HHHHHHHCCCCCHHH	25.26	26643407
668	Phosphorylation	TLRRSMSTEGNKRGM HHHHHHCCCCCHHHH	38.99	26643407
673	Dimethylation	MSTEGNKRGMIQLIV HCCCCCHHHHHHHHH	43.86	-
673	Methylation	MSTEGNKRGMIQLIV HCCCCCHHHHHHHHH	43.86	18965593
682	Dimethylation	MIQLIVARRISRCNE HHHHHHHHHHHHCHH	26.10	-
682	Methylation	MIQLIVARRISRCNE HHHHHHHHHHHHCHH	26.10	18965603
692	Phosphorylation	SRCNELRSPGSPAAP HHCHHCCCCCCCCCC	46.36	25619855
695	Phosphorylation	NELRSPGSPAAPELP HHCCCCCCCCCCCCC	18.10	26824392
705	Phosphorylation	APELPIETELDDRER CCCCCCCEECCHHHH	42.44	25619855
714	Phosphorylation	LDDRERRISHSLYSG CCHHHHHHHHHHHHC	5.64	-
715	Phosphorylation	DDRERRISHSLYSGI CHHHHHHHHHHHHCC	12.76	25521595
717	Phosphorylation	RERRISHSLYSGIEG HHHHHHHHHHHCCCC	23.39	21082442
719	Phosphorylation	RRISHSLYSGIEGLD HHHHHHHHHCCCCCC	14.10	18515860
720	Phosphorylation	RISHSLYSGIEGLDE HHHHHHHHCCCCCCC	38.29	26239621
724	Ubiquitination	SLYSGIEGLDESPTR HHHHCCCCCCCCCCH	36.19	-
728	Phosphorylation	GIEGLDESPTRNAAL CCCCCCCCCCHHHHH	31.10	25521595
728 (in isoform 3)	Phosphorylation	-	31.10	30352176
730	Phosphorylation	EGLDESPTRNAALSR CCCCCCCCHHHHHHH	45.92	26239621
806	Phosphorylation	DSADCSLSPDVDPVL CCCCCCCCCCCCHHH	11.62	29899451
824	Phosphorylation	REGFGRQSMSEKRTK CCCCCCCCCCHHHHH	23.94	26824392
826	Phosphorylation	GFGRQSMSEKRTKQF CCCCCCCCHHHHHCC	45.25	26643407
830	Phosphorylation	QSMSEKRTKQFSDAS CCCCHHHHHCCCCHH	40.02	18267089
831	Acetylation	SMSEKRTKQFSDASQ CCCHHHHHCCCCHHH	54.07	21949390
831	Ubiquitination	SMSEKRTKQFSDASQ CCCHHHHHCCCCHHH	54.07	-
834	Phosphorylation	EKRTKQFSDASQLDF HHHHHCCCCHHHCHH	29.76	26643407
837	Phosphorylation	TKQFSDASQLDFVKT HHCCCCHHHCHHHHC	35.95	28066266
843	Ubiquitination	ASQLDFVKTRKSKSM HHHCHHHHCCCCCCC	42.48	-
848	Acetylation	FVKTRKSKSMDLVAD HHHCCCCCCCCCEEC	53.48	21949390
849	Phosphorylation	VKTRKSKSMDLVADE HHCCCCCCCCCEECC	25.31	28066266
861	Phosphorylation	ADETKLNTVDDQRAG ECCCCCCCCCCCCCC	35.43	20469934
869	Phosphorylation	VDDQRAGSPSRDVGP CCCCCCCCCCCCCCH	20.79	26824392
871	Phosphorylation	DQRAGSPSRDVGPSL CCCCCCCCCCCCHHC	42.69	20469934
877	Phosphorylation	PSRDVGPSLGLKKSS CCCCCCHHCCCCCCC	29.19	27149854
881	Acetylation	VGPSLGLKKSSSLES CCHHCCCCCCCCHHH	49.13	21949390
883	Phosphorylation	PSLGLKKSSSLESLQ HHCCCCCCCCHHHHH	24.46	21082442
884	Phosphorylation	SLGLKKSSSLESLQT HCCCCCCCCHHHHHH	48.07	21183079
885	Phosphorylation	LGLKKSSSLESLQTA CCCCCCCCHHHHHHH	43.16	21082442
888	Phosphorylation	KKSSSLESLQTAVAE CCCCCHHHHHHHEEE	30.94	22817900
891	Phosphorylation	SSLESLQTAVAEVTL CCHHHHHHHEEEECC	28.52	17242355
920	Phosphorylation	RGRGCNESFRAAIDK CCCCCCHHHHHHHCC	13.17	27087446
928	Phosphorylation	FRAAIDKSYDKPMVD HHHHHCCCCCCCCCC	34.51	22817900
929	Phosphorylation	RAAIDKSYDKPMVDD HHHHCCCCCCCCCCC	33.45	22817900
943	Phosphorylation	DDDEGMETLEEDTEE CCCCCCCCHHHHCHH	30.49	29899451
954	Phosphorylation	DTEESSRSGRESVST HCHHHHHCCCCCCCC	44.12	25619855
958	Phosphorylation	SSRSGRESVSTSSDQ HHHCCCCCCCCCCCC	21.73	25619855
960	Phosphorylation	RSGRESVSTSSDQPS HCCCCCCCCCCCCCC	31.67	25619855
961	Phosphorylation	SGRESVSTSSDQPSY CCCCCCCCCCCCCCC	30.05	25619855
962	Phosphorylation	GRESVSTSSDQPSYS CCCCCCCCCCCCCCC	25.14	25619855
963	Phosphorylation	RESVSTSSDQPSYSL CCCCCCCCCCCCCCH	40.11	25619855
967	Phosphorylation	STSSDQPSYSLERQM CCCCCCCCCCHHHHH	23.01	28066266
969	Phosphorylation	SSDQPSYSLERQMNG CCCCCCCCHHHHHCC	28.26	26824392
1042	Phosphorylation	GRIKIQDSFTSEEDR CCEEECCCCCCHHHH	17.66	26824392
1044	Phosphorylation	IKIQDSFTSEEDRVR EEECCCCCCHHHHHH	38.90	28066266
1045	Phosphorylation	KIQDSFTSEEDRVRM EECCCCCCHHHHHHH	36.66	28066266
1076	Phosphorylation	RQARERDYAEIQDFH HHHHHHCHHHHHHHH	16.62	26824392
1085	Phosphorylation	EIQDFHRTFGCDDEL HHHHHHHHHCCCCCC	18.79	27087446
1112	Phosphorylation	ALNARPQSPREGHLM HHCCCCCCCCCCCCH	28.71	27087446
1121	Phosphorylation	REGHLMDTLYAQVKK CCCCCHHHHHHHCCC	14.01	22499769
1123	Phosphorylation	GHLMDTLYAQVKKPR CCCHHHHHHHCCCCC	9.33	20116462
1170	Phosphorylation	DVEDRRRTYSFEQSW CHHHHHHHCCHHHHH	23.00	23984901
1171	Phosphorylation	VEDRRRTYSFEQSWS HHHHHHHCCHHHHHC	15.00	26643407
1172	Phosphorylation	EDRRRTYSFEQSWSS HHHHHHCCHHHHHCC	22.99	29514104
1176	Phosphorylation	RTYSFEQSWSSSRPA HHCCHHHHHCCCCCC	23.02	26643407
1178	Phosphorylation	YSFEQSWSSSRPASQ CCHHHHHCCCCCCCC	24.55	26643407
1179	Phosphorylation	SFEQSWSSSRPASQS CHHHHHCCCCCCCCC	24.90	26643407
1180	Phosphorylation	FEQSWSSSRPASQSG HHHHHCCCCCCCCCC	36.45	26643407
1184	Phosphorylation	WSSSRPASQSGRHSV HCCCCCCCCCCCCCE	27.80	26643407
1186	Phosphorylation	SSRPASQSGRHSVSV CCCCCCCCCCCCEEE	34.99	26643407
1190	Phosphorylation	ASQSGRHSVSVEVQV CCCCCCCCEEEEHHH	18.09	26370283
1207	Phosphorylation	QRQEERESFQQAQRQ HHHHHHHHHHHHHHH	34.28	26824392
1215	Phosphorylation	FQQAQRQYSSLPRQS HHHHHHHHHCCCHHH	11.47	22499769
1216	Phosphorylation	QQAQRQYSSLPRQSR HHHHHHHHCCCHHHH	19.63	22499769
1217	Phosphorylation	QAQRQYSSLPRQSRK HHHHHHHCCCHHHHC	37.14	22499769
1222	Phosphorylation	YSSLPRQSRKNASSI HHCCCHHHHCCCCCC	46.64	29514104
1227	Phosphorylation	RQSRKNASSISQDSW HHHHCCCCCCCCCHH	37.28	23984901
1228	Phosphorylation	QSRKNASSISQDSWE HHHCCCCCCCCCHHH	24.69	26643407
1230	Phosphorylation	RKNASSISQDSWEQN HCCCCCCCCCHHHHH	29.86	23984901
1233	Phosphorylation	ASSISQDSWEQNYAP CCCCCCCHHHHHCCC	25.52	23984901
1238	Phosphorylation	QDSWEQNYAPGEGFQ CCHHHHHCCCCCCCC	17.54	22817900
1255	Phosphorylation	KENPRYSSYQGSRNG CCCCCCCCCCCCCCC	16.73	25521595
1256	Phosphorylation	ENPRYSSYQGSRNGY CCCCCCCCCCCCCCC	15.79	29514104
1263	Phosphorylation	YQGSRNGYLGGHGFN CCCCCCCCCCCCCCC	13.01	29514104
1312	Phosphorylation	FRQDVPPSPSQVARL CCCCCCCCHHHHHHH	30.98	26239621
1314	Phosphorylation	QDVPPSPSQVARLNR CCCCCCHHHHHHHHC	41.44	25619855
1324	Phosphorylation	ARLNRLQTPEKGRPF HHHHCCCCCCCCCCC	37.70	25338131
1327	Acetylation	NRLQTPEKGRPFYS- HCCCCCCCCCCCCC-	62.51	21949390
1333	Phosphorylation	EKGRPFYS------- CCCCCCCC-------	33.84	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
830	T	Phosphorylation	Kinase	ROCK1	P70335	PSP
958	S	Phosphorylation	Kinase	AURKA	P97477	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
824	S	Phosphorylation	-
Phosphorylation at Ser-824 by PRKCZ and PRKCI occurs at the most apical tip of epithelial cell-cell contacts during the initial phase of tight junction formation and may promote dissociation of the complex with PARD6.
958	S	Phosphorylation	-
Phosphorylation by AURKA at Ser-958 is required for the normal establishment of neuronal polarity (By similarity).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PARD3_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference	Domain-Domain Interactions
JAM1_HUMAN	F11R	physical	11447115
PAR6A_MOUSE	Pard6a	physical	16510873

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PARD3_MOUSE

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry."; Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.; J. Proteome Res. 7:5314-5326(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-383 AND SER-728, ANDMASS SPECTROMETRY.	19367708 [Abstract]
"Large-scale phosphorylation analysis of mouse liver."; Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-144 AND SER-717, ANDMASS SPECTROMETRY.	17242355 [Abstract]

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