RRAS2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RRAS2_MOUSE
• UniProt AC: P62071
• Protein Name: Ras-related protein R-Ras2
• Gene Name: Rras2
• Organism: Mus musculus (Mouse).
• Sequence Length: 204
• Subcellular Localization: Cell membrane; Lipid-anchor; Cytoplasmic side. Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).
• Protein Description: It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion..
• Protein Sequence: MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDTAGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVTQEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAAGWRDG ------CCCCCCCCC	13.15	-
10	Phosphorylation	AAGWRDGSGQEKYRL CCCCCCCCCCCEEEE	41.41	25266776
105	Phosphorylation	RGSFEEIYKFQRQIL CCCHHHHHHHHHHHH	14.72	-
106	Ubiquitination	GSFEEIYKFQRQILR CCHHHHHHHHHHHHC	40.45	22790023
128	Ubiquitination	PMILIGNKADLDHQR CEEEEECHHHCCHHH	37.76	22790023
177	Ubiquitination	ELVRVIRKFQEQECP HHHHHHHHHHHCCCC	40.03	-
186	Phosphorylation	QEQECPPSPEPTRKE HHCCCCCCCCCCCCC	27.34	27087446
190	Phosphorylation	CPPSPEPTRKEKDKK CCCCCCCCCCCCCCC	53.35	27742792
194	Acetylation	PEPTRKEKDKKGCHC CCCCCCCCCCCCCEE	77.28	7721145
196	Acetylation	PTRKEKDKKGCHCVI CCCCCCCCCCCEEEE	63.23	7721157
199	S-palmitoylation	KEKDKKGCHCVIF-- CCCCCCCCEEEEC--	2.86	-
201	Methylation	KDKKGCHCVIF---- CCCCCCEEEEC----	2.67	-
201	Farnesylation	KDKKGCHCVIF---- CCCCCCEEEEC----	2.67	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of RRAS2_MOUSE !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RRAS2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RRAS2_MOUSE !!

Protein-Protein Interaction

Oops, there are no PPI records of RRAS2_MOUSE !!

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"The phagosomal proteome in interferon-gamma-activated macrophages.";
Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,Thibault P.;
Immunity 30:143-154(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND MASSSPECTROMETRY.
PubMed: 19144319

"Solid tumor proteome and phosphoproteome analysis by high resolutionmass spectrometry.";
Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J.,Faessler R., Mann M.;
J. Proteome Res. 7:5314-5326(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND MASSSPECTROMETRY.
PubMed: 19367708

"Qualitative and quantitative analyses of protein phosphorylation innaive and stimulated mouse synaptosomal preparations.";
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F.,Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D.,Gerrits B., Panse C., Schlapbach R., Mansuy I.M.;
Mol. Cell. Proteomics 6:283-293(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND MASSSPECTROMETRY.
PubMed: 17114649

"Protein phosphorylation and expression profiling by Yin-yangmultidimensional liquid chromatography (Yin-yang MDLC) massspectrometry.";
Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R.;
J. Proteome Res. 6:250-262(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, AND MASSSPECTROMETRY.
PubMed: 17203969