WWP2_MOUSE - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: WWP2_MOUSE
• UniProt AC: Q9DBH0
• Protein Name: NEDD4-like E3 ubiquitin-protein ligase WWP2
• Gene Name: Wwp2
• Organism: Mus musculus (Mouse).
• Sequence Length: 870
• Subcellular Localization: Nucleus .
• Protein Description: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; regulates POU5F1 protein level during differentiation of embryonal carcinoma cells (ECCs) but not in undifferentiated ECCs and embryonic stem cells (ESCs). Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation..
• Protein Sequence: MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEIIVLNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENTQLTLNLQTENKGSVVSGGELTIFLDGPTVDLGSVPNGSAVTDGSQPPSRESSGTAIAPETRHQPPSTNCFGGRSRTHRHSGGSARTATAASEQSPGARNRHRQPVKNSSSSGLANGTVNEEPTPASEPEESSVVGVTSLPAAALSVSSNPNTTSLPAQSTPAEGEEASTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVDHNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRVYYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGAYDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIAREWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPFYKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELFFIQDMEILGKVTTHELKEGGENIRVTEENKEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKNAIYRHYTKSSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDRVGKETWLPRSHTCFNRLDLPPYKSYEQLKEKLLYAIEETEGFGQE

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
18	Phosphorylation	VALPFEKSQLTLKVV CCCCCCHHCEEEEEE	24.19	28059163
21	Phosphorylation	PFEKSQLTLKVVSAK CCCHHCEEEEEEECC	19.52	28059163
186	Glutathionylation	HQPPSTNCFGGRSRT CCCCCCCCCCCCCCC	3.21	24333276
203	Phosphorylation	HSGGSARTATAASEQ CCCCCCHHHHHHHCC	28.24	25777480
205	Phosphorylation	GGSARTATAASEQSP CCCCHHHHHHHCCCC	23.34	25777480
208	Phosphorylation	ARTATAASEQSPGAR CHHHHHHHCCCCCCC	33.43	29899451
211	Phosphorylation	ATAASEQSPGARNRH HHHHHCCCCCCCCCC	22.65	25521595
369	Phosphorylation	TAEYVRNYEQWQSQR CHHHHHCHHHHHHHH	10.03	-
487	Phosphorylation	ESGTKQGSPGAYDRS CCCCCCCCCCCCCCC	20.05	29899451
499	Phosphorylation	DRSFRWKYHQFRFLC CCCCCEEEEEEEHHH	8.22	-
508	Phosphorylation	QFRFLCHSNALPSHV EEEHHHCCCCCCCCE	23.85	25777480
513	Phosphorylation	CHSNALPSHVKISVS HCCCCCCCCEEEEEC	41.59	25777480
518	Phosphorylation	LPSHVKISVSRQTLF CCCCEEEEECCCHHH	14.34	24719451
520	Phosphorylation	SHVKISVSRQTLFED CCEEEEECCCHHHHH	16.33	25777480
523	Phosphorylation	KISVSRQTLFEDSFQ EEEECCCHHHHHHHH	32.10	25777480
528	Phosphorylation	RQTLFEDSFQQIMNM CCHHHHHHHHHHHCC	20.11	25777480
538	Phosphorylation	QIMNMKPYDLRRRLY HHHCCCHHCHHHEEE	23.30	25777480
828	Ubiquitination	FCIDRVGKETWLPRS EEEECCCCCCCCCCC	49.46	22790023

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	Wwp2	Q9DBH0	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of WWP2_MOUSE !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of WWP2_MOUSE !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
GSC_MOUSE	Gsc	physical	21170031
EGR2_MOUSE	Egr2	physical	19651900
PO5F1_MOUSE	Pou5f1	physical	15047715
SMRC1_MOUSE	Smarcc1	physical	24365151
SOX2_MOUSE	Sox2	physical	25042802
RPB1_MOUSE	Polr2a	physical	17526739
SMRC1_MOUSE	Smarcc1	physical	17526739
UB2L3_MOUSE	Ube2l3	physical	21170031

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.