dbPTM

PJA1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PJA1_HUMAN
UniProt AC	Q8NG27
Protein Name	E3 ubiquitin-protein ligase Praja-1
Gene Name	PJA1
Organism	Homo sapiens (Human).
Sequence Length	643
Subcellular Localization
Protein Description	Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome (By similarity). May be involved in protein sorting..
Protein Sequence	MGQESSKPVWPNPTGGYQSNTGRRYGRRHAYVSFRPPTSQRERIASQRKTNSEVPMHRSAPSQTTKRSRSPFSTTRRSWDDSESSGTNLNIDNEDYSRYPPREYRASGSRRGMAYGHIDSYGADDSEEEGAGPVERPPVRGKTGKFKDDKLYDPEKGARSLAGPPPHFSSFSRDVREERDKLDPVPAARCSASRADFLPQSSVASQSSSEGKLATKGDSSERERREQNLPARPSRAPVSICGGGENTSKSAEEPVVRPKIRNLASPNCVKPKIFFDTDDDDDMPHSTSRWRDTANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Ubiquitination	ESSKPVWPNPTGGYQ CCCCCCCCCCCCCCC	36.84	22053931
50	Phosphorylation	RIASQRKTNSEVPMH HHHHHCCCCCCCCCC	46.28	22210691
52	Phosphorylation	ASQRKTNSEVPMHRS HHHCCCCCCCCCCCC	45.24	22210691
59	Phosphorylation	SEVPMHRSAPSQTTK CCCCCCCCCCCCCCC	29.83	22199227
62	Phosphorylation	PMHRSAPSQTTKRSR CCCCCCCCCCCCCCC	39.62	24425749
64	Phosphorylation	HRSAPSQTTKRSRSP CCCCCCCCCCCCCCC	38.33	22199227
65	Phosphorylation	RSAPSQTTKRSRSPF CCCCCCCCCCCCCCC	19.47	22210691
66 (in isoform 2)	Ubiquitination	-	52.33	21890473
66	Ubiquitination	SAPSQTTKRSRSPFS CCCCCCCCCCCCCCC	52.33	22053931
68	Phosphorylation	PSQTTKRSRSPFSTT CCCCCCCCCCCCCCC	38.37	30266825
70 (in isoform 2)	Phosphorylation	-	31.38	-
70	Phosphorylation	QTTKRSRSPFSTTRR CCCCCCCCCCCCCCC	31.38	23911959
71	Phosphorylation	TTKRSRSPFSTTRRS CCCCCCCCCCCCCCC	26.54	32142685
73	Phosphorylation	KRSRSPFSTTRRSWD CCCCCCCCCCCCCCC	32.05	23927012
74	Phosphorylation	RSRSPFSTTRRSWDD CCCCCCCCCCCCCCC	25.38	23186163
75	Phosphorylation	SRSPFSTTRRSWDDS CCCCCCCCCCCCCCC	23.92	23186163
78	Phosphorylation	PFSTTRRSWDDSESS CCCCCCCCCCCCCCC	31.40	28348404
87	Phosphorylation	DDSESSGTNLNIDNE CCCCCCCCCCCCCCC	38.18	-
115	Phosphorylation	GSRRGMAYGHIDSYG CCCCCCCCCCCCCCC	10.59	29978859
120	Phosphorylation	MAYGHIDSYGADDSE CCCCCCCCCCCCCCC	25.51	29978859
121	Phosphorylation	AYGHIDSYGADDSEE CCCCCCCCCCCCCCC	16.76	30576142
126	Phosphorylation	DSYGADDSEEEGAGP CCCCCCCCCCCCCCC	47.67	23401153
149	Ubiquitination	KTGKFKDDKLYDPEK CCCCCCCCCCCCHHH	43.37	20972266
169	Phosphorylation	AGPPPHFSSFSRDVR CCCCCCHHHCCHHHH	27.69	24719451
179	Phosphorylation	SRDVREERDKLDPVP CHHHHHHHHCCCCCC	41.43	33259812
194	Ubiquitination	AARCSASRADFLPQS CHHHCCCCCCCCCCC	37.58	32015554
210	Phosphorylation	VASQSSSEGKLATKG CCCCCCCCCCCCCCC	63.40	32142685
211	Ubiquitination	ASQSSSEGKLATKGD CCCCCCCCCCCCCCC	32.02	32015554
212	Acetylation	SQSSSEGKLATKGDS CCCCCCCCCCCCCCH	30.78	25953088
222	Phosphorylation	TKGDSSERERREQNL CCCCHHHHHHHHHCC	44.67	32142685
249	Ubiquitination	GGGENTSKSAEEPVV CCCCCCCCCCCCCCC	52.20	32015554
250	Phosphorylation	GGENTSKSAEEPVVR CCCCCCCCCCCCCCC	40.45	28348404
265	O-linked_Glycosylation	PKIRNLASPNCVKPK HHHHHCCCCCCCCCE	21.58	30379171
265	Phosphorylation	PKIRNLASPNCVKPK HHHHHCCCCCCCCCE	21.58	23401153
277	Phosphorylation	KPKIFFDTDDDDDMP CCEEECCCCCCCCCC	35.85	29255136
282	Ubiquitination	FDTDDDDDMPHSTSR CCCCCCCCCCCCCCC	60.55	20972266
286	Phosphorylation	DDDDMPHSTSRWRDT CCCCCCCCCCCCCCC	23.39	23403867
287	Phosphorylation	DDDMPHSTSRWRDTA CCCCCCCCCCCCCCC	21.19	23403867
288	Phosphorylation	DDMPHSTSRWRDTAN CCCCCCCCCCCCCCC	32.62	23403867
301	Phosphorylation	ANDNEGHSDGLARRG CCCCCCCCCCCHHCC	44.20	29449344
312	Phosphorylation	ARRGRGESSSGYPEP HHCCCCCCCCCCCCC	32.40	25394399
313	Phosphorylation	RRGRGESSSGYPEPK HCCCCCCCCCCCCCC	24.39	23312004
314	Phosphorylation	RGRGESSSGYPEPKY CCCCCCCCCCCCCCC	52.18	23312004
316	Phosphorylation	RGESSSGYPEPKYPE CCCCCCCCCCCCCCH	13.33	-
337	Ubiquitination	SDQVKPEKVPRRRRT HCCCCHHHCCCCCCC	67.10	20972266
344	Ubiquitination	KVPRRRRTMADPDFW HCCCCCCCCCCCCCC	18.20	32015554
344	Phosphorylation	KVPRRRRTMADPDFW HCCCCCCCCCCCCCC	18.20	27251275
354	Phosphorylation	DPDFWTHSDDYYKYC CCCCCCCCCCHHHHC	25.46	-
360	Phosphorylation	HSDDYYKYCDEDSDS CCCCHHHHCCCCCCC	6.76	22617229
365	Phosphorylation	YKYCDEDSDSDKEWI HHHCCCCCCCCHHHH	36.65	22617229
367	Phosphorylation	YCDEDSDSDKEWIAA HCCCCCCCCHHHHHH	55.31	22617229
381	Phosphorylation	ALRRKYRSREQTLSS HHHHHHHCHHHHHCC	36.76	24719451
385	Phosphorylation	KYRSREQTLSSSGES HHHCHHHHHCCCCCC	24.69	25850435
387	Phosphorylation	RSREQTLSSSGESWE HCHHHHHCCCCCCCC	26.33	25850435
388	Phosphorylation	SREQTLSSSGESWET CHHHHHCCCCCCCCC	45.54	20363803
389	Phosphorylation	REQTLSSSGESWETL HHHHHCCCCCCCCCC	43.11	25850435
392	Phosphorylation	TLSSSGESWETLPGK HHCCCCCCCCCCCCC	33.46	20363803
399	Ubiquitination	SWETLPGKEEREPPQ CCCCCCCCCCCCCCC	55.23	32015554
440 (in isoform 2)	Ubiquitination	-	19.72	21890473
440	Ubiquitination	AGSNGSNYLEEVREP CCCCCCCHHHHHCCC	19.72	21890473
440	Phosphorylation	AGSNGSNYLEEVREP CCCCCCCHHHHHCCC	19.72	-
448	Phosphorylation	LEEVREPSLQEEQAS HHHHCCCCHHHHHHH	37.62	-
537	Phosphorylation	VDPQFLTYMALEERL CCHHHHHHHHHHHHH	5.20	22817900
557	Phosphorylation	TALAHLESLAVDVEV HHHHHHHHHCCEEEE	28.17	24275569
573	Ubiquitination	NPPASKESIDALPEI CCCCCHHHHHCCCCE	29.51	21890473
573	Ubiquitination	NPPASKESIDALPEI CCCCCHHHHHCCCCE	29.51	21890473
628	Ubiquitination	CVSIWLQKSGTCPVC CEEEECCCCCCCCCC	49.00	22817900
628 (in isoform 1)	Ubiquitination	-	49.00	21890473

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of PJA1_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PJA1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PJA1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MAGD1_HUMAN	MAGED1	physical	11959851
SPTB2_HUMAN	SPTBN1	physical	16247473
UB2D2_HUMAN	UBE2D2	physical	12036302
EZH2_HUMAN	EZH2	physical	21513699
SUZ12_HUMAN	SUZ12	physical	21513699
EED_HUMAN	EED	physical	21513699
PJA1_HUMAN	PJA1	physical	21513699
UBP20_HUMAN	USP20	physical	22626734
UBP51_HUMAN	USP51	physical	22626734
UBP5_HUMAN	USP5	physical	22626734
ATX3_HUMAN	ATXN3	physical	22626734
UBP7_HUMAN	USP7	physical	22626734
STABP_HUMAN	STAMBP	physical	22626734
OTUB1_HUMAN	OTUB1	physical	22626734
JOS1_HUMAN	JOSD1	physical	22626734
UCHL3_HUMAN	UCHL3	physical	22626734
UBP8_HUMAN	USP8	physical	22626734
UCHL5_HUMAN	UCHL5	physical	22626734
OTUB2_HUMAN	OTUB2	physical	22626734
ATX3L_HUMAN	ATXN3L	physical	22626734
UBP28_HUMAN	USP28	physical	22626734
ESPL1_HUMAN	ESPL1	physical	22626734
STAM1_HUMAN	STAM	physical	22626734
UB2D3_HUMAN	UBE2D3	physical	22626734
UB2L3_HUMAN	UBE2L3	physical	21513699
PJA1_HUMAN	PJA1	physical	12036302
MAGD4_HUMAN	MAGED4B	physical	28514442
SMC5_HUMAN	SMC5	physical	28514442
SMC6_HUMAN	SMC6	physical	28514442
NSE4A_HUMAN	NSMCE4A	physical	28514442
NSE3_HUMAN	NDNL2	physical	28514442
CHRC1_HUMAN	CHRAC1	physical	28514442
UBP7_HUMAN	USP7	physical	28514442
FAF2_HUMAN	FAF2	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PJA1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-277, AND MASSSPECTROMETRY.	19690332 [Abstract]