NSE4A_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NSE4A_HUMAN
• UniProt AC: Q9NXX6
• Protein Name: Non-structural maintenance of chromosomes element 4 homolog A
• Gene Name: NSMCE4A
• Organism: Homo sapiens (Human).
• Sequence Length: 385
• Subcellular Localization: Nucleus . Chromosome, telomere .
• Protein Description: Component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Is involved in positive regulation of response to DNA damage stimulus..
• Protein Sequence: MSGDSSGRGPEGRGRGRDPHRDRTRSRSRSRSPLSPRSRRGSARERREAPERPSLEDTEPSDSGDEMMDPASLEAEADQGLCRQIRHQYRALINSVQQNREDILNAGDKLTEVLEEANTLFNEVSRAREAVLDAHFLVLASDLGKEKAKQLRSDLSSFDMLRYVETLLTHMGVNPLEAEELIRDEDSPDFEFIVYDSWKITGRTAENTFNKTHTFHFLLGSIYGECPVPKPRVDRPRKVPVIQEERAMPAQLRRMEESHQEATEKEVERILGLLQTYFREDPDTPMSFFDFVVDPHSFPRTVENIFHVSFIIRDGFARIRLDQDRLPVIEPVSINEENEGFEHNTQVRNQGIIALSYRDWEEIVKTFEISEPVITPSQRQQKPSA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Phosphorylation	------MSGDSSGRG ------CCCCCCCCC	51.84	24043423
5	Phosphorylation	---MSGDSSGRGPEG ---CCCCCCCCCCCC	37.91	24043423
6	Phosphorylation	--MSGDSSGRGPEGR --CCCCCCCCCCCCC	36.93	24043423
26	Phosphorylation	PHRDRTRSRSRSRSP CCCCCCCCCCCCCCC	33.71	24719451
28	Phosphorylation	RDRTRSRSRSRSPLS CCCCCCCCCCCCCCC	35.54	20363803
30	Phosphorylation	RTRSRSRSRSPLSPR CCCCCCCCCCCCCCC	38.05	23401153
32	Phosphorylation	RSRSRSRSPLSPRSR CCCCCCCCCCCCCCC	31.29	23401153
35	Phosphorylation	SRSRSPLSPRSRRGS CCCCCCCCCCCCCCC	22.96	26055452
38	Phosphorylation	RSPLSPRSRRGSARE CCCCCCCCCCCCHHH	29.70	24275569
38	O-linked_Glycosylation	RSPLSPRSRRGSARE CCCCCCCCCCCCHHH	29.70	30379171
54	Phosphorylation	REAPERPSLEDTEPS HCCCCCCCCCCCCCC	51.08	22115753
58	Phosphorylation	ERPSLEDTEPSDSGD CCCCCCCCCCCCCCC	40.18	22115753
61	Phosphorylation	SLEDTEPSDSGDEMM CCCCCCCCCCCCCCC	37.87	22617229
63	Phosphorylation	EDTEPSDSGDEMMDP CCCCCCCCCCCCCCH	53.10	22617229
72	Phosphorylation	DEMMDPASLEAEADQ CCCCCHHHHHHHHHH	31.93	25072903
79	Ubiquitination	SLEAEADQGLCRQIR HHHHHHHHHHHHHHH	54.53	22817900
95	Phosphorylation	QYRALINSVQQNRED HHHHHHHHHHHCHHH	17.82	28555341
106	Ubiquitination	NREDILNAGDKLTEV CHHHHHHHHHHHHHH	24.90	22817900
111	Phosphorylation	LNAGDKLTEVLEEAN HHHHHHHHHHHHHHH	29.77	26074081
125	Phosphorylation	NTLFNEVSRAREAVL HHHHHHHHHHHHHHH	17.65	26074081
145	Ubiquitination	VLASDLGKEKAKQLR HHHHHHCHHHHHHHH	64.44	33845483
147	Ubiquitination	ASDLGKEKAKQLRSD HHHHCHHHHHHHHHH	65.45	-
204	Phosphorylation	SWKITGRTAENTFNK CEEEECCCHHHCCCC	39.40	20068231
206	Ubiquitination	KITGRTAENTFNKTH EEECCCHHHCCCCCE	57.07	22817900
238 (in isoform 2)	Ubiquitination	-	54.28	21906983
238 (in isoform 1)	Ubiquitination	-	54.28	21906983
238	Ubiquitination	PRVDRPRKVPVIQEE CCCCCCCCCCCCCHH	54.28	21906983
258	Phosphorylation	QLRRMEESHQEATEK HHHHHHHHHHHHHHH	19.71	-
263	Phosphorylation	EESHQEATEKEVERI HHHHHHHHHHHHHHH	47.00	-
265	Ubiquitination	SHQEATEKEVERILG HHHHHHHHHHHHHHH	63.81	21906983
265 (in isoform 2)	Ubiquitination	-	63.81	21906983
265 (in isoform 1)	Ubiquitination	-	63.81	21906983
301	Phosphorylation	DPHSFPRTVENIFHV CCCCCCCHHHHHHEE	32.20	24719451
333	Phosphorylation	LPVIEPVSINEENEG CCEEECEECCCCCCC	30.34	29255136
345	Phosphorylation	NEGFEHNTQVRNQGI CCCCCCCHHHHCCCE	30.28	17525332
365	Ubiquitination	RDWEEIVKTFEISEP CCHHHHHHHCEECCC	53.83	22817900
365 (in isoform 1)	Ubiquitination	-	53.83	21906983
370	Phosphorylation	IVKTFEISEPVITPS HHHHCEECCCCCCHH	28.75	30266825
375	Phosphorylation	EISEPVITPSQRQQK EECCCCCCHHHHCCC	19.68	30266825
377	Phosphorylation	SEPVITPSQRQQKPS CCCCCCHHHHCCCCC	29.37	17525332
381	Ubiquitination	ITPSQRQQKPSA--- CCHHHHCCCCCC---	62.91	29967540
382	Ubiquitination	TPSQRQQKPSA---- CHHHHCCCCCC----	32.38	29967540
384	Phosphorylation	SQRQQKPSA------ HHHCCCCCC------	54.36	22067460

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NSE4A_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NSE4A_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NSE4A_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
NSE1_HUMAN	NSMCE1	physical	18086888
SMC6_HUMAN	SMC6	physical	18086888
SMC6_HUMAN	SMC6	physical	26186194
NSE3_HUMAN	NDNL2	physical	26186194
SMC5_HUMAN	SMC5	physical	26186194
BRCA2_HUMAN	BRCA2	physical	26186194
DNJB2_HUMAN	DNAJB2	physical	26186194
NSE3_HUMAN	NDNL2	physical	28514442
SMC6_HUMAN	SMC6	physical	28514442
DNJB2_HUMAN	DNAJB2	physical	28514442
SMC5_HUMAN	SMC5	physical	28514442
BRCA2_HUMAN	BRCA2	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage.";
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
Science 316:1160-1166(2007).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-345 AND SER-377, ANDMASS SPECTROMETRY.
PubMed: 17525332