dbPTM

SIK3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SIK3_HUMAN
UniProt AC	Q9Y2K2
Protein Name	Serine/threonine-protein kinase SIK3 {ECO:0000305}
Gene Name	SIK3 {ECO:0000312\|HGNC:HGNC:29165}
Organism	Homo sapiens (Human).
Sequence Length	1321
Subcellular Localization	Cytoplasm . Locates to punctate structures within the cytoplasm on binding to YWHAZ.
Protein Description
Protein Sequence	MAAAAASGAGGAAGAGTGGAGPAGRLLPPPAPGSPAAPAAVSPAAGQPRPPAPASRGPMPARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQICKHKWMKLGDADPNFDRLIAECQQLKEERQVDPLNEDVLLAMEDMGLDKEQTLQSLRSDAYDHYSAIYSLLCDRHKRHKTLRLGALPSMPRALAFQAPVNIQAEQAGTAMNISVPQVQLINPENQIVEPDGTLNLDSDEGEEPSPEALVRYLSMRRHTVGVADPRTEVMEDLQKLLPGFPGVNPQAPFLQVAPNVNFMHNLLPMQNLQPTGQLEYKEQSLLQPPTLQLLNGMGPLGRRASDGGANIQLHAQQLLKRPRGPSPLVTMTPAVPAVTPVDEESSDGEPDQEAVQSSTYKDSNTLHLPTERFSPVRRFSDGAASIQAFKAHLEKMGNNSSIKQLQQECEQLQKMYGGQIDERTLEKTQQQHMLYQQEQHHQILQQQIQDSICPPQPSPPLQAACENQPALLTHQLQRLRIQPSSPPPNHPNNHLFRQPSNSPPPMSSAMIQPHGAASSSQFQGLPSRSAIFQQQPENCSSPPNVALTCLGMQQPAQSQQVTIQVQEPVDMLSNMPGTAAGSSGRGISISPSAGQMQMQHRTNLMATLSYGHRPLSKQLSADSAEAHSLNVNRFSPANYDQAHLHPHLFSDQSRGSPSSYSPSTGVGFSPTQALKVPPLDQFPTFPPSAHQQPPHYTTSALQQALLSPTPPDYTRHQQVPHILQGLLSPRHSLTGHSDIRLPPTEFAQLIKRQQQQRQQQQQQQQQQEYQELFRHMNQGDAGSLAPSLGGQSMTERQALSYQNADSYHHHTSPQHLLQIRAQECVSQASSPTPPHGYAHQPALMHSESMEEDCSCEGAKDGFQDSKSSSTLTKGCHDSPLLLSTGGPGDPESLLGTVSHAQELGIHPYGHQPTAAFSKNKVPSREPVIGNCMDRSSPGQAVELPDHNGLGYPARPSVHEHHRPRALQRHHTIQNSDDAYVQLDNLPGMSLVAGKALSSARMSDAVLSQSSLMGSQQFQDGENEECGASLGGHEHPDLSDGSQHLNSSCYPSTCITDILLSYKHPEVSFSMEQAGV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
1	Acetylation	-------MAAAAASG -------CCHHHHCC	28.13	-
13	Phosphorylation	ASGAGGAAGAGTGGA HCCCCCCCCCCCCCC	30.27	19413330
16	Ubiquitination	AGGAAGAGTGGAGPA CCCCCCCCCCCCCCC	46.99	-
23	Acetylation	GTGGAGPAGRLLPPP CCCCCCCCCCCCCCC	29.71	-
30	Phosphorylation	AGRLLPPPAPGSPAA CCCCCCCCCCCCCCC	31.48	24719451
34	Phosphorylation	LPPPAPGSPAAPAAV CCCCCCCCCCCCCCC	8.76	26055452
34	Phosphorylation	LPPPAPGSPAAPAAV CCCCCCCCCCCCCCC	8.76	-
41	Ubiquitination	SPAAPAAVSPAAGQP CCCCCCCCCCCCCCC	27.97	-
41	Ubiquitination	SPAAPAAVSPAAGQP CCCCCCCCCCCCCCC	27.97	-
42	Phosphorylation	PAAPAAVSPAAGQPR CCCCCCCCCCCCCCC	30.53	26425664
42	Phosphorylation	PAAPAAVSPAAGQPR CCCCCCCCCCCCCCC	30.53	26425664
50	Ubiquitination	PAAGQPRPPAPASRG CCCCCCCCCCCCCCC	56.24	-
50	Ubiquitination	PAAGQPRPPAPASRG CCCCCCCCCCCCCCC	56.24	-
70 (in isoform 2)	Phosphorylation	-	2.18	19690332
71	Phosphorylation	GYYEIDRTIGKGNFA EEEEEECCCCCCCEE	6.82	19369195
72 (in isoform 2)	Phosphorylation	-	32.45	19690332
76 (in isoform 2)	Phosphorylation	-	15.70	19690332
84	Phosphorylation	FAVVKRATHLVTKAK EEEEEHHHHHHHHHH	22.23	22210691
88	Phosphorylation	KRATHLVTKAKVAIK EHHHHHHHHHHHHHE	46.56	22210691
113 (in isoform 6)	Phosphorylation	-	38.22	19690332
132	Ubiquitination	IIRLYQVMETERMIY HHHHHHHHHHHHHHH	58.92	-
132	Ubiquitination	IIRLYQVMETERMIY HHHHHHHHHHHHHHH	58.92	-
156	Phosphorylation	EIFDHLVAHGRMAEK HHHHHHHHCCHHCHH	29.67	19369195
163	Phosphorylation	AHGRMAEKEARRKFK HCCHHCHHHHHHHHH	26.42	14976552
167	Phosphorylation	MAEKEARRKFKQIVT HCHHHHHHHHHHHHH	21.40	23403867
170	Phosphorylation	KEARRKFKQIVTAVY HHHHHHHHHHHHHHH	20.84	23403867
221	Ubiquitination	TPGQLLKTWCGSPPY CHHHHHHHHCCCCCC	28.26	-
221	Phosphorylation	TPGQLLKTWCGSPPY CHHHHHHHHCCCCCC	28.26	14976552
257	Ubiquitination	GVVLYVLVCGALPFD HHHHHHHHHCCCCCC	34.18	-
260	Ubiquitination	LYVLVCGALPFDGST HHHHHHCCCCCCCHH	48.48	-
260	Ubiquitination	LYVLVCGALPFDGST HHHHHHCCCCCCCHH	48.48	-
279	Ubiquitination	RARVLSGKFRIPFFM HHHHHCCCCCCCEEE	53.87	-
279	Ubiquitination	RARVLSGKFRIPFFM HHHHHCCCCCCCEEE	53.87	-
390	Phosphorylation	CDRHKRHKTLRLGAL CHHHHHCCEEECCCC	40.50	24275569
404	Phosphorylation	LPSMPRALAFQAPVN CCCCCCEEHHCCCCC	8.82	18083107
411	Phosphorylation	LAFQAPVNIQAEQAG EHHCCCCCEEHHHHC	19.69	23401153
466 (in isoform 4)	Phosphorylation	-	9.09	23927012
469	Phosphorylation	YLSMRRHTVGVADPR HHHHHCCCCCCCCCC	39.64	24275569
470 (in isoform 4)	Phosphorylation	-	44.49	23927012
472	Phosphorylation	MRRHTVGVADPRTEV HHCCCCCCCCCCHHH	31.94	23882029
472 (in isoform 4)	Phosphorylation	-	31.94	23927012
478	Phosphorylation	GVADPRTEVMEDLQK CCCCCCHHHHHHHHH	33.54	23882029
479 (in isoform 4)	Phosphorylation	-	4.10	26657352
485	Phosphorylation	EVMEDLQKLLPGFPG HHHHHHHHHCCCCCC	4.93	18669648
485 (in isoform 4)	Phosphorylation	-	4.93	23927012
486 (in isoform 4)	Phosphorylation	-	18.71	23927012
493	Phosphorylation	LLPGFPGVNPQAPFL HCCCCCCCCCCCCCE	34.67	29255136
507 (in isoform 4)	Phosphorylation	-	3.72	28122231
511 (in isoform 4)	Phosphorylation	-	73.93	28122231
514	Phosphorylation	NFMHNLLPMQNLQPT CCHHHCCCCCCCCCC	33.42	23090842
514 (in isoform 4)	Phosphorylation	-	33.42	28122231
518	Phosphorylation	NLLPMQNLQPTGQLE HCCCCCCCCCCCCCC	24.02	30177828
520	Phosphorylation	LPMQNLQPTGQLEYK CCCCCCCCCCCCCCC	9.95	22210691
524	Phosphorylation	NLQPTGQLEYKEQSL CCCCCCCCCCCHHHC	24.10	23927012
524	Phosphorylation	NLQPTGQLEYKEQSL CCCCCCCCCCCHHHC	24.10	23090842
527	Phosphorylation	PTGQLEYKEQSLLQP CCCCCCCCHHHCCCC	21.50	23090842
528	Phosphorylation	TGQLEYKEQSLLQPP CCCCCCCHHHCCCCC	25.59	23927012
530	Phosphorylation	QLEYKEQSLLQPPTL CCCCCHHHCCCCCCH	55.97	23927012
533	Phosphorylation	YKEQSLLQPPTLQLL CCHHHCCCCCCHHHH	31.51	18669648
534	Phosphorylation	KEQSLLQPPTLQLLN CHHHCCCCCCHHHHC	53.57	25850435
537	Phosphorylation	SLLQPPTLQLLNGMG HCCCCCCHHHHCCCC	51.17	26657352
537	Phosphorylation	SLLQPPTLQLLNGMG HCCCCCCHHHHCCCC	51.17	23090842
543	Phosphorylation	TLQLLNGMGPLGRRA CHHHHCCCCCCCCCC	5.81	23927012
543	Phosphorylation	TLQLLNGMGPLGRRA CHHHHCCCCCCCCCC	5.81	23090842
544	Phosphorylation	LQLLNGMGPLGRRAS HHHHCCCCCCCCCCC	38.42	23927012
544	Phosphorylation	LQLLNGMGPLGRRAS HHHHCCCCCCCCCCC	38.42	25850435
545	Phosphorylation	QLLNGMGPLGRRASD HHHCCCCCCCCCCCC	20.32	29449344
546	Phosphorylation	LLNGMGPLGRRASDG HHCCCCCCCCCCCCC	20.75	29449344
547	Phosphorylation	LNGMGPLGRRASDGG HCCCCCCCCCCCCCC	39.58	29449344
548	Phosphorylation	NGMGPLGRRASDGGA CCCCCCCCCCCCCCC	18.10	29449344
551	Phosphorylation	GPLGRRASDGGANIQ CCCCCCCCCCCCHHH	27.80	19369195
553	Phosphorylation	LGRRASDGGANIQLH CCCCCCCCCCHHHHH	21.36	23186163
558	Phosphorylation	SDGGANIQLHAQQLL CCCCCHHHHHHHHHH	46.26	30576142
562	Phosphorylation	ANIQLHAQQLLKRPR CHHHHHHHHHHCCCC	28.80	30576142
565	Phosphorylation	QLHAQQLLKRPRGPS HHHHHHHHCCCCCCC	36.99	28122231
568	Phosphorylation	AQQLLKRPRGPSPLV HHHHHCCCCCCCCCC	33.07	18669648
568	Phosphorylation	AQQLLKRPRGPSPLV HHHHHCCCCCCCCCC	33.07	29255136
569	Phosphorylation	QQLLKRPRGPSPLVT HHHHCCCCCCCCCCE	48.30	28122231
572	Phosphorylation	LKRPRGPSPLVTMTP HCCCCCCCCCCEECC	16.82	28122231
573	Phosphorylation	KRPRGPSPLVTMTPA CCCCCCCCCCEECCC	18.07	26846344
578	Ubiquitination	PSPLVTMTPAVPAVT CCCCCEECCCCCCCC	37.60	-
588	Phosphorylation	VPAVTPVDEESSDGE CCCCCCCCCCCCCCC	37.12	23401153
589	Phosphorylation	PAVTPVDEESSDGEP CCCCCCCCCCCCCCC	35.61	23401153
591	Phosphorylation	VTPVDEESSDGEPDQ CCCCCCCCCCCCCCH	47.07	-
592	Phosphorylation	TPVDEESSDGEPDQE CCCCCCCCCCCCCHH	42.72	-
604	Phosphorylation	DQEAVQSSTYKDSNT CHHHHHHHCCCCCCC	25.74	-
626	Phosphorylation	FSPVRRFSDGAASIQ CCCCCCCCCCHHHHH	36.03	20166139
647	Phosphorylation	EKMGNNSSIKQLQQE HHCCCCHHHHHHHHH	40.06	-
672	Phosphorylation	QIDERTLEKTQQQHM CCCHHHHHHHHHHHH	38.82	30266825
673	Phosphorylation	IDERTLEKTQQQHML CCHHHHHHHHHHHHH	48.38	23401153
688	Phosphorylation	YQQEQHHQILQQQIQ HHHHHHHHHHHHHHH	41.42	29523821
690	Phosphorylation	QEQHHQILQQQIQDS HHHHHHHHHHHHHHH	43.02	29523821
695	Phosphorylation	QILQQQIQDSICPPQ HHHHHHHHHHCCCCC	22.25	27080861
696	Phosphorylation	ILQQQIQDSICPPQP HHHHHHHHHCCCCCC	18.66	27080861
776	Phosphorylation	QGLPSRSAIFQQQPE CCCCCHHHHHCCCCC	21.60	21406692
778	Phosphorylation	LPSRSAIFQQQPENC CCCHHHHHCCCCCCC	13.15	21406692
780	Phosphorylation	SRSAIFQQQPENCSS CHHHHHCCCCCCCCC	37.94	21406692
795	Phosphorylation	PPNVALTCLGMQQPA CCCEEEHHCCCCCCC	11.41	27251275
797	Phosphorylation	NVALTCLGMQQPAQS CEEEHHCCCCCCCCC	25.39	27251275
804	Phosphorylation	GMQQPAQSQQVTIQV CCCCCCCCCEEEEEE	22.49	28111955
808	Phosphorylation	PAQSQQVTIQVQEPV CCCCCEEEEEECCCH	27.77	29255136
811	Phosphorylation	SQQVTIQVQEPVDML CCEEEEEECCCHHHH	27.94	22496350
816	Phosphorylation	IQVQEPVDMLSNMPG EEECCCHHHHHCCCC	28.78	23403867
844	Phosphorylation	PSAGQMQMQHRTNLM CCCCHHHHCCCCCHH	22.09	25159151
846	Phosphorylation	AGQMQMQHRTNLMAT CCHHHHCCCCCHHHH	43.54	25159151
847	Phosphorylation	GQMQMQHRTNLMATL CHHHHCCCCCHHHHH	32.77	25159151
848	Phosphorylation	QMQMQHRTNLMATLS HHHHCCCCCHHHHHH	29.01	27732954
849	Phosphorylation	MQMQHRTNLMATLSY HHHCCCCCHHHHHHC	17.83	27732954
851	Phosphorylation	MQHRTNLMATLSYGH HCCCCCHHHHHHCCC	31.67	26657352
852	Phosphorylation	QHRTNLMATLSYGHR CCCCCHHHHHHCCCC	38.99	27732954
857	Phosphorylation	LMATLSYGHRPLSKQ HHHHHHCCCCCHHHC	22.55	26657352
859	Phosphorylation	ATLSYGHRPLSKQLS HHHHCCCCCHHHCCC	25.85	25159151
866	Phosphorylation	RPLSKQLSADSAEAH CCHHHCCCCCCHHHH	52.11	19369195
916	Phosphorylation	STGVGFSPTQALKVP CCCCCCCCCCCCCCC	25.57	22167270
920	Phosphorylation	GFSPTQALKVPPLDQ CCCCCCCCCCCCHHH	28.98	23401153
922	Phosphorylation	SPTQALKVPPLDQFP CCCCCCCCCCHHHCC	35.54	28985074
925	Phosphorylation	QALKVPPLDQFPTFP CCCCCCCHHHCCCCC	35.76	27251275
928	Methylation	KVPPLDQFPTFPPSA CCCCHHHCCCCCCCC	46.24	-
932	Phosphorylation	LDQFPTFPPSAHQQP HHHCCCCCCCCCCCC	41.62	28348404
957	Phosphorylation	ALLSPTPPDYTRHQQ HHHCCCCCCCCCCCH	11.71	28796482
978	Phosphorylation	GLLSPRHSLTGHSDI HHCCCCCCCCCCCCC	22.98	24275569
986	Methylation	LTGHSDIRLPPTEFA CCCCCCCCCCHHHHH	12.51	-
994	Phosphorylation	LPPTEFAQLIKRQQQ CCHHHHHHHHHHHHH	24.77	24719451
995	Phosphorylation	PPTEFAQLIKRQQQQ CHHHHHHHHHHHHHH	13.01	27080861
999	Phosphorylation	FAQLIKRQQQQRQQQ HHHHHHHHHHHHHHH	37.43	27080861
1000	Phosphorylation	AQLIKRQQQQRQQQQ HHHHHHHHHHHHHHH	20.43	27080861
1014	Phosphorylation	QQQQQQQEYQELFRH HHHHHHHHHHHHHHH	31.52	27080861
1017	Phosphorylation	QQQQEYQELFRHMNQ HHHHHHHHHHHHHHC	29.25	27080861
1018	Phosphorylation	QQQEYQELFRHMNQG HHHHHHHHHHHHHCC	35.49	27080861
1020	Phosphorylation	QEYQELFRHMNQGDA HHHHHHHHHHHCCCH	51.94	27080861
1025	Phosphorylation	LFRHMNQGDAGSLAP HHHHHHCCCHHHCCH	9.62	27080861
1034	Phosphorylation	AGSLAPSLGGQSMTE HHHCCHHHCCCCCCH	19.87	23401153
1036	Phosphorylation	SLAPSLGGQSMTERQ HCCHHHCCCCCCHHH	34.21	25002506
1101	Phosphorylation	SMEEDCSCEGAKDGF CCCCCCCCCCCCCCC	24.85	-
1111	Phosphorylation	AKDGFQDSKSSSTLT CCCCCCCCCCCCCCC	52.87	-
1159	Phosphorylation	HPYGHQPTAAFSKNK CCCCCCCCHHHCCCC	20.38	29978859
1163	Phosphorylation	HQPTAAFSKNKVPSR CCCCHHHCCCCCCCC	22.08	24719451
1167	Phosphorylation	AAFSKNKVPSREPVI HHHCCCCCCCCCCCC	8.84	29978859
1177	Phosphorylation	REPVIGNCMDRSSPG CCCCCCCCCCCCCCC	26.13	27080861
1185	Phosphorylation	MDRSSPGQAVELPDH CCCCCCCCCEECCCC	27.15	24719451
1186	Phosphorylation	DRSSPGQAVELPDHN CCCCCCCCEECCCCC	22.27	26699800
1249	Phosphorylation	LSSARMSDAVLSQSS HHHHHHHHHHHCCHH	17.18	-
1255	Phosphorylation	SDAVLSQSSLMGSQQ HHHHHCCHHHHCCCC	15.22	28555341

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
163	T	Phosphorylation	Kinase	STK11	Q15831	PhosphoELM
221	T	Phosphorylation	Kinase	LKB1	Q15831	Uniprot
221	T	Phosphorylation	Kinase	STK11	Q15831	GPS
411	T	Phosphorylation	Kinase	PHKG2	Q9DB30	GPS
469	T	Phosphorylation	Kinase	PKACA	P17612	PSP
469	T	Phosphorylation	Kinase	PHKG2	Q9DB30	PSP
493	S	Phosphorylation	Kinase	PHKG2	Q9DB30	GPS
551	S	Phosphorylation	Kinase	PKACA	P17612	PSP
551	S	Phosphorylation	Kinase	PHKG2	Q9DB30	PSP
626	S	Phosphorylation	Kinase	PKACA	P17612	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
221	T	Phosphorylation	14976552
Phosphorylated at Thr-221 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.
221	T	Phosphorylation	14976552
Phosphorylation at Thr-221 is inhibited in response to PTHLH/PTHrP.
469	T	Phosphorylation	23186163
Phosphorylated at Thr-469 and Ser-551 in response to cAMP signaling (Probable).
551	S	Phosphorylation	19369195
Phosphorylated at Thr-469 and Ser-551 in response to cAMP signaling (Probable).

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SIK3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TAB2_HUMAN	TAB2	physical	24061540
M3K7_HUMAN	MAP3K7	physical	24061540
HDAC5_HUMAN	HDAC5	physical	23393134
1433Z_HUMAN	YWHAZ	physical	22588126
1433T_HUMAN	YWHAQ	physical	22588126
1433E_HUMAN	YWHAE	physical	22588126
1433B_HUMAN	YWHAB	physical	22588126
1433F_HUMAN	YWHAH	physical	22588126
1433S_HUMAN	SFN	physical	22588126
TRIP6_HUMAN	TRIP6	physical	21516116

Drug and Disease Associations

Kegg Disease
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SIK3_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND MASS SPECTROMETRY.	19413330 [Abstract]
Phosphorylation
Reference	PubMed
"Large-scale proteomics analysis of the human kinome."; Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.; Mol. Cell. Proteomics 8:1751-1764(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-13; THR-156; THR-163;SER-493; THR-527; SER-533; SER-568; SER-672 AND SER-808, AND MASSSPECTROMETRY.	19369195 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-568, AND MASSSPECTROMETRY.	18669648 [Abstract]
"LKB1 is a master kinase that activates 13 kinases of the AMPKsubfamily, including MARK/PAR-1."; Lizcano J.M., Goeransson O., Toth R., Deak M., Morrice N.A.,Boudeau J., Hawley S.A., Udd L., Maekelae T.P., Hardie D.G.,Alessi D.R.; EMBO J. 23:833-843(2004). Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME REGULATION,PHOSPHORYLATION AT THR-163, AND MUTAGENESIS OF THR-163.	14976552 [Abstract]

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