TGIF1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TGIF1_HUMAN
• UniProt AC: Q15583
• Protein Name: Homeobox protein TGIF1
• Gene Name: TGIF1
• Organism: Homo sapiens (Human).
• Sequence Length: 401
• Subcellular Localization: Nucleus.
• Protein Description: Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities..
• Protein Sequence: MVLAQSRVSAGVGSPHCSGSGGGGSDSFPWPASHPGNPQCSFSTAFLASPRLSRGTLAYLPPAPWSSLATPSALLGSSCAPPPPPARCPQPRALSPELGTKAGPRRPHRWELPRSPSQGAQGPAPRRRLLETMKGIVAASGSETEDEDSMDIPLDLSSSAGSGKRRRRGNLPKESVQILRDWLYEHRYNAYPSEQEKALLSQQTHLSTLQVCNWFINARRRLLPDMLRKDGKDPNQFTISRRGAKISETSSVESVMGIKNFMPALEETPFHSCTAGPNPTLGRPLSPKPSSPGSVLARPSVICHTTVTALKDVPFSLCQSVGVGQNTDIQQIAAKNFTDTSLMYPEDTCKSGPSTNTQSGLFNTPPPTPPDLNQDFSGFQLLVDVALKRAAEMELQAKLTA

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
83	Ubiquitination	GSSCAPPPPPARCPQ CCCCCCCCCCCCCCC	46.58	33845483
95	Phosphorylation	CPQPRALSPELGTKA CCCCCCCCHHHCCCC	18.49	25850435
100	Phosphorylation	ALSPELGTKAGPRRP CCCHHHCCCCCCCCC	30.28	23532336
103	Ubiquitination	PELGTKAGPRRPHRW HHHCCCCCCCCCCCC	20.15	33845483
106	Ubiquitination	GTKAGPRRPHRWELP CCCCCCCCCCCCCCC	34.21	33845483
115	Phosphorylation	HRWELPRSPSQGAQG CCCCCCCCCCCCCCC	27.28	23403867
117	Ubiquitination	WELPRSPSQGAQGPA CCCCCCCCCCCCCCH	42.36	33845483
117	Phosphorylation	WELPRSPSQGAQGPA CCCCCCCCCCCCCCH	42.36	23403867
140	Phosphorylation	MKGIVAASGSETEDE HHHHHHCCCCCCCCC	32.87	22777824
142	Phosphorylation	GIVAASGSETEDEDS HHHHCCCCCCCCCCC	38.56	19664994
144	Phosphorylation	VAASGSETEDEDSMD HHCCCCCCCCCCCCC	50.89	22777824
149	Phosphorylation	SETEDEDSMDIPLDL CCCCCCCCCCCCCCC	19.12	21406692
157 (in isoform 2)	Phosphorylation	-	23.16	-
157	Phosphorylation	MDIPLDLSSSAGSGK CCCCCCCCCCCCCCC	23.16	19664994
158	Phosphorylation	DIPLDLSSSAGSGKR CCCCCCCCCCCCCCC	31.35	28102081
159	Phosphorylation	IPLDLSSSAGSGKRR CCCCCCCCCCCCCCC	33.02	25841592
162 (in isoform 2)	Phosphorylation	-	40.64	-
162	Phosphorylation	DLSSSAGSGKRRRRG CCCCCCCCCCCCCCC	40.64	19664994
165 (in isoform 2)	Phosphorylation	-	40.64	-
171 (in isoform 3)	Phosphorylation	-	7.74	-
176 (in isoform 3)	Phosphorylation	-	4.26	-
179 (in isoform 3)	Phosphorylation	-	1.96	-
232	Ubiquitination	DMLRKDGKDPNQFTI HHHHHCCCCCCCCCC	78.95	29967540
232	Acetylation	DMLRKDGKDPNQFTI HHHHHCCCCCCCCCC	78.95	26051181
238	Phosphorylation	GKDPNQFTISRRGAK CCCCCCCCCCCCCCC	14.31	28555341
247	Phosphorylation	SRRGAKISETSSVES CCCCCCCCCCCCHHH	33.83	28555341
251	Phosphorylation	AKISETSSVESVMGI CCCCCCCCHHHHHCC	37.01	27251275
268	Phosphorylation	FMPALEETPFHSCTA HHHHHHCCCCCCCCC	23.14	27251275
272	Phosphorylation	LEETPFHSCTAGPNP HHCCCCCCCCCCCCC	17.34	27251275
274	Phosphorylation	ETPFHSCTAGPNPTL CCCCCCCCCCCCCCC	37.35	27251275
280	Phosphorylation	CTAGPNPTLGRPLSP CCCCCCCCCCCCCCC	48.78	28348404
286	Phosphorylation	PTLGRPLSPKPSSPG CCCCCCCCCCCCCCC	33.29	19664994
290	Phosphorylation	RPLSPKPSSPGSVLA CCCCCCCCCCCCCCC	56.12	28152594
291	Phosphorylation	PLSPKPSSPGSVLAR CCCCCCCCCCCCCCC	41.63	19664994
294	Phosphorylation	PKPSSPGSVLARPSV CCCCCCCCCCCCCCE	19.83	19664994

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	FBXW7	Q969H0	PMID:24658274
-	K	Ubiquitination	E3 ubiquitin ligase	ITCH	Q96J02	PMID:22199232

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TGIF1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TGIF1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MKKS_HUMAN	MKKS	physical	17353931
SDA1_HUMAN	SDAD1	physical	17353931
STK16_HUMAN	STK16	physical	17353931
CTBP1_HUMAN	CTBP1	physical	10995736
SMAD3_HUMAN	SMAD3	physical	11427533
SMAD2_HUMAN	SMAD2	physical	10199400
SMAD1_HUMAN	SMAD1	physical	10199400
LRIF1_HUMAN	LRIF1	physical	16169070
MED31_HUMAN	MED31	physical	16169070
EF1A1_HUMAN	EEF1A1	physical	16169070
JUN_HUMAN	JUN	physical	11371641
SIN3A_HUMAN	SIN3A	physical	11682623
A2MG_HUMAN	A2M	physical	21900206
PML_HUMAN	PML	physical	18511908
PML_HUMAN	PML	physical	16916642
A4_HUMAN	APP	physical	21832049

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 142946: Holoprosencephaly 4 (HPE4)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.