MED31_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MED31_HUMAN
• UniProt AC: Q9Y3C7
• Protein Name: Mediator of RNA polymerase II transcription subunit 31
• Gene Name: MED31
• Organism: Homo sapiens (Human).
• Sequence Length: 131
• Subcellular Localization: Nucleus .
• Protein Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors..
• Protein Sequence: MAAAVAMETDDAGNRLRFQLELEFVQCLANPNYLNFLAQRGYFKDKAFVNYLKYLLYWKDPEYAKYLKYPQCLHMLELLQYEHFRKELVNAQCAKFIDEQQILHWQHYSRKRMRLQQALAEQQQQNNTSGK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MAAAVAMET ------CCCEEEEEC	12.25	22814378
9	Phosphorylation	AAAVAMETDDAGNRL CCEEEEECCCCCCCE	26.54	-
65	Ubiquitination	WKDPEYAKYLKYPQC CCCHHHHHHCCCHHH	50.65	29967540
69	Phosphorylation	EYAKYLKYPQCLHML HHHHHCCCHHHHHHH	8.92	-
95	Ubiquitination	LVNAQCAKFIDEQQI HHHHHHHHHCCHHHH	50.94	29967540
128	Phosphorylation	EQQQQNNTSGK---- HHHHHHCCCCC----	46.54	28555341
129	Phosphorylation	QQQQNNTSGK----- HHHHHCCCCC-----	46.86	17192257
131	Acetylation	QQNNTSGK------- HHHCCCCC-------	57.86	30590379
131	Ubiquitination	QQNNTSGK------- HHHCCCCC-------	57.86	24816145

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MED31_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MED31_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MED31_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
MED19_HUMAN	MED19	physical	12584197
ZSCA1_HUMAN	ZSCAN1	physical	20211142
HD_HUMAN	HTT	physical	15383276

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Phosphorylation

"Large-scale proteomics analysis of the human kinome.";
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,Mann M., Daub H.;
Mol. Cell. Proteomics 8:1751-1764(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129, AND MASSSPECTROMETRY.
PubMed: 19369195

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129, AND MASSSPECTROMETRY.
PubMed: 18691976