dbPTM

TSC2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	TSC2_HUMAN
UniProt AC	P49815
Protein Name	Tuberin
Gene Name	TSC2
Organism	Homo sapiens (Human).
Sequence Length	1807
Subcellular Localization	Cytoplasm. Membrane Peripheral membrane protein. At steady state found in association with membranes.
Protein Description	In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. [PubMed: 12271141]
Protein Sequence	MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Ubiquitination	-----MAKPTSKDSG -----CCCCCCCCCC	61.77	-
7	Acetylation	-MAKPTSKDSGLKEK -CCCCCCCCCCHHHH	59.39	12433467
7	Ubiquitination	-MAKPTSKDSGLKEK -CCCCCCCCCCHHHH	59.39	-
12	Acetylation	TSKDSGLKEKFKILL CCCCCCHHHHHHHHH	63.48	12433485
14	Ubiquitination	KDSGLKEKFKILLGL CCCCHHHHHHHHHCC	50.82	-
16	Ubiquitination	SGLKEKFKILLGLGT CCHHHHHHHHHCCCC	43.44	-
20	Ubiquitination	EKFKILLGLGTPRPN HHHHHHHCCCCCCCC	20.72	-
22	Ubiquitination	FKILLGLGTPRPNPR HHHHHCCCCCCCCCC	31.69	-
23	Ubiquitination	KILLGLGTPRPNPRS HHHHCCCCCCCCCCC	22.59	-
23	Phosphorylation	KILLGLGTPRPNPRS HHHHCCCCCCCCCCC	22.59	22199227
30	Phosphorylation	TPRPNPRSAEGKQTE CCCCCCCCCCCCCEE	31.74	29514088
34	Ubiquitination	NPRSAEGKQTEFIIT CCCCCCCCCEEEEEE	45.62	-
57	Ubiquitination	MECGLNNRIRMIGQI HHCCCCHHHHHHHHH	19.47	-
69	Acetylation	GQICEVAKTKKFEEH HHHHHHHCCCCHHHH	67.08	30593117
69	Ubiquitination	GQICEVAKTKKFEEH HHHHHHHCCCCHHHH	67.08	-
71	Ubiquitination	ICEVAKTKKFEEHAV HHHHHCCCCHHHHHH	55.55	-
72	Ubiquitination	CEVAKTKKFEEHAVE HHHHCCCCHHHHHHH	64.34	21890473
72	Ubiquitination	CEVAKTKKFEEHAVE HHHHCCCCHHHHHHH	64.34	21890473
72	Ubiquitination	CEVAKTKKFEEHAVE HHHHCCCCHHHHHHH	64.34	21890473
72	Ubiquitination	CEVAKTKKFEEHAVE HHHHCCCCHHHHHHH	64.34	21890473
72 (in isoform 1)	Ubiquitination	-	64.34	21890473
72 (in isoform 2)	Ubiquitination	-	64.34	21890473
72 (in isoform 3)	Ubiquitination	-	64.34	21890473
72 (in isoform 4)	Ubiquitination	-	64.34	21890473
72 (in isoform 5)	Ubiquitination	-	64.34	21890473
79	Ubiquitination	KFEEHAVEALWKAVA CHHHHHHHHHHHHHH	38.54	-
95	Ubiquitination	LLQPERPLEARHAVL HHCCCCCHHHHHHHH	11.43	-
106	Ubiquitination	HAVLALLKAIVQGQG HHHHHHHHHHHCCCH	36.60	-
125	Ubiquitination	VLRALFFKVIKDYPS HHHHHHHHHHCCCCC	35.40	-
128	Ubiquitination	ALFFKVIKDYPSNED HHHHHHHCCCCCCHH	54.95	-
144	Ubiquitination	HERLEVFKALTDNGR HHHHHHHHHHHCCCC	47.89	21906983
144 (in isoform 1)	Ubiquitination	-	47.89	21890473
144 (in isoform 2)	Ubiquitination	-	47.89	21890473
144 (in isoform 3)	Ubiquitination	-	47.89	21890473
144 (in isoform 4)	Ubiquitination	-	47.89	21890473
147	Phosphorylation	LEVFKALTDNGRHIT HHHHHHHHCCCCEEE	32.07	29954749
188	Phosphorylation	VNLVKFNSCYLDEYI HHHHCCCCCCHHHHH	13.79	-
201	Ubiquitination	YIARMVQMICLLCVR HHHHHHHHHHHHHHH	1.27	-
209	Ubiquitination	ICLLCVRTASSVDIE HHHHHHHCCCCCCCC	15.53	-
250	Ubiquitination	LCRTINVKELCEPCW HHCCCCHHHHHHHHH	40.24	-
258	Ubiquitination	ELCEPCWKLMRNLLG HHHHHHHHHHHHHHH	38.84	-
311	Phosphorylation	WGAHRLYSLRNSPTS HCHHHHHHHHCCCCC	25.99	24719451
389	Ubiquitination	TIVHDLLTTVEELCD HHHHHHHHHHHHHHH	35.77	-
401 (in isoform 6)	Ubiquitination	-	28.90	21890473
438	Ubiquitination	AQSIHPAKDGWIQNL HCCCCCCCCCHHHHH	62.31	21890473
438	Ubiquitination	AQSIHPAKDGWIQNL HCCCCCCCCCHHHHH	62.31	21890473
438	Ubiquitination	AQSIHPAKDGWIQNL HCCCCCCCCCHHHHH	62.31	21890473
438 (in isoform 1)	Ubiquitination	-	62.31	21890473
438 (in isoform 2)	Ubiquitination	-	62.31	21890473
438 (in isoform 3)	Ubiquitination	-	62.31	21890473
438 (in isoform 4)	Ubiquitination	-	62.31	21890473
438 (in isoform 5)	Ubiquitination	-	62.31	21890473
540	Phosphorylation	KVMARSLSPPPELEE HHHHHHCCCCCCHHH	36.29	22817900
585	Ubiquitination	LPASHATRVYEMLVS CCCHHHHHHHHHHHH	29.78	-
598	Phosphorylation	VSHIQLHYKHSYTLP HHHHHCCCCCCCCCC	20.99	23917254
601	Phosphorylation	IQLHYKHSYTLPIAS HHCCCCCCCCCCCCC	18.70	23917254
602	Phosphorylation	QLHYKHSYTLPIASS HCCCCCCCCCCCCCH	16.41	20068231
603	Phosphorylation	LHYKHSYTLPIASSI CCCCCCCCCCCCCHH	29.13	20068231
608	Phosphorylation	SYTLPIASSIRLQAF CCCCCCCCHHHHHHH	27.33	20068231
609	Ubiquitination	YTLPIASSIRLQAFD CCCCCCCHHHHHHHH	11.60	-
609	Phosphorylation	YTLPIASSIRLQAFD CCCCCCCHHHHHHHH	11.60	23917254
634	Ubiquitination	HRLGLPNKDGVVRFS HHCCCCCCCCEEEEC	54.65	-
634 (in isoform 2)	Ubiquitination	-	54.65	-
658	Ubiquitination	PERGSEKKTSGPLSP CCCCCCCCCCCCCCC	43.82	-
658 (in isoform 2)	Ubiquitination	-	43.82	-
659	Phosphorylation	ERGSEKKTSGPLSPP CCCCCCCCCCCCCCC	50.40	28450419
660	Phosphorylation	RGSEKKTSGPLSPPT CCCCCCCCCCCCCCC	47.49	28450419
664	Phosphorylation	KKTSGPLSPPTGPPG CCCCCCCCCCCCCCC	31.78	25867546
667	Phosphorylation	SGPLSPPTGPPGPAP CCCCCCCCCCCCCCC	66.75	28464451
704	Ubiquitination	LKQESDWKVLKLVLG HHCCCCHHHHHHHHC	42.55	-
733	Ubiquitination	SPCSVDQLCSALCSM CCCCHHHHHHHHHHH	1.84	-
751	Methylation	PKTLERLRGAPEGFS HHHHHHHCCCCCCCC	45.51	115919061
760	Phosphorylation	APEGFSRTDLHLAVV CCCCCCCHHHHHHHH	41.54	27251275
771	Phosphorylation	LAVVPVLTALISYHN HHHHHHHHHHHHHHH	21.47	27251275
775	Phosphorylation	PVLTALISYHNYLDK HHHHHHHHHHHCCCH	22.61	27251275
776	Phosphorylation	VLTALISYHNYLDKT HHHHHHHHHHCCCHH	6.26	27251275
779	Phosphorylation	ALISYHNYLDKTKQR HHHHHHHCCCHHCHH	11.72	27251275
782	Ubiquitination	SYHNYLDKTKQREMV HHHHCCCHHCHHHHH	55.84	-
865	Ubiquitination	RNFAAEQYASVFAIS HHHCHHHHHHEEEEE	7.73	-
877 (in isoform 6)	Ubiquitination	-	32.76	21890473
881	Ubiquitination	PYTNPSKFNQYIVCL CCCCHHHHHHHHHHH	8.93	-
907 (in isoform 7)	Phosphorylation	-	45.58	29507054
914	Ubiquitination	DFVPFITKGLRSNVL CCHHHHCCCCCCCEE	51.65	21890473
914	Ubiquitination	DFVPFITKGLRSNVL CCHHHHCCCCCCCEE	51.65	21890473
914	Ubiquitination	DFVPFITKGLRSNVL CCHHHHCCCCCCCEE	51.65	21890473
914 (in isoform 1)	Ubiquitination	-	51.65	21890473
914 (in isoform 2)	Ubiquitination	-	51.65	21890473
914 (in isoform 3)	Ubiquitination	-	51.65	21890473
914 (in isoform 4)	Ubiquitination	-	51.65	21890473
914 (in isoform 5)	Ubiquitination	-	51.65	21890473
918	Phosphorylation	FITKGLRSNVLLSFD HHCCCCCCCEEEECC	36.01	23312004
919 (in isoform 6)	Phosphorylation	-	23.25	29507054
923	Phosphorylation	LRSNVLLSFDDTPEK CCCCEEEECCCCCCC	23.59	29396449
924	Ubiquitination	RSNVLLSFDDTPEKD CCCEEEECCCCCCCC	11.27	-
927	Phosphorylation	VLLSFDDTPEKDSFR EEEECCCCCCCCCCC	34.88	25159151
930	Ubiquitination	SFDDTPEKDSFRARS ECCCCCCCCCCCCCC	60.93	2190698
930 (in isoform 1)	Ubiquitination	-	60.93	21890473
930 (in isoform 2)	Ubiquitination	-	60.93	21890473
930 (in isoform 3)	Ubiquitination	-	60.93	21890473
930 (in isoform 4)	Ubiquitination	-	60.93	21890473
932	Phosphorylation	DDTPEKDSFRARSTS CCCCCCCCCCCCCCC	27.92	29978859
937	Phosphorylation	KDSFRARSTSLNERP CCCCCCCCCCCCCCC	22.74	25159151
937 (in isoform 3)	Phosphorylation	-	22.74	25627689
937 (in isoform 5)	Phosphorylation	-	22.74	25627689
938	Phosphorylation	DSFRARSTSLNERPK CCCCCCCCCCCCCCC	31.51	29514088
939	Phosphorylation	SFRARSTSLNERPKS CCCCCCCCCCCCCCC	30.37	25159151
939 (in isoform 3)	Phosphorylation	-	30.37	29116813
939 (in isoform 5)	Phosphorylation	-	30.37	29116813
946	Phosphorylation	SLNERPKSLRIARPP CCCCCCCCCCCCCCC	26.09	-
951	Ubiquitination	PKSLRIARPPKQGLN CCCCCCCCCCCCCCC	45.76	-
954	Ubiquitination	LRIARPPKQGLNNSP CCCCCCCCCCCCCCC	60.73	-
956 (in isoform 2)	Phosphorylation	-	36.18	29507054
960	Phosphorylation	PKQGLNNSPPVKEFK CCCCCCCCCCHHHHH	29.52	25627689
964	Ubiquitination	LNNSPPVKEFKESSA CCCCCCHHHHHHCCH	64.31	-
966	Ubiquitination	NSPPVKEFKESSAAE CCCCHHHHHHCCHHH	9.85	-
967	Methylation	SPPVKEFKESSAAEA CCCHHHHHHCCHHHH	58.75	115979451
979	Phosphorylation	AEAFRCRSISVSEHV HHHHHCCEEEHHHHH	23.88	29255136
981	Phosphorylation	AFRCRSISVSEHVVR HHHCCEEEHHHHHHH	22.34	29255136
983	Phosphorylation	RCRSISVSEHVVRSR HCCEEEHHHHHHHHH	18.39	29978859
993	Phosphorylation	VVRSRIQTSLTSASL HHHHHHHHHHCCCCC	24.27	28450419
994	Phosphorylation	VRSRIQTSLTSASLG HHHHHHHHHCCCCCC	17.18	28450419
996	Phosphorylation	SRIQTSLTSASLGSA HHHHHHHCCCCCCCC	23.24	28450419
997	Phosphorylation	RIQTSLTSASLGSAD HHHHHHCCCCCCCCC	22.58	19690332
999	Phosphorylation	QTSLTSASLGSADEN HHHHCCCCCCCCCCC	32.79	23403867
1002	Phosphorylation	LTSASLGSADENSVA HCCCCCCCCCCCHHH	37.53	28450419
1007	Phosphorylation	LGSADENSVAQADDS CCCCCCCHHHCCCHH	19.44	28450419
1014	Ubiquitination	SVAQADDSLKNLHLE HHHCCCHHHHHHHHH	41.62	-
1014	Phosphorylation	SVAQADDSLKNLHLE HHHCCCHHHHHHHHH	41.62	27251275
1019	Ubiquitination	DDSLKNLHLELTETC CHHHHHHHHHHHHHH	27.01	-
1043	Ubiquitination	SNFTAVPKRSPVGEF HCCCCCCCCCCCCCE	59.93	-
1045	Phosphorylation	FTAVPKRSPVGEFLL CCCCCCCCCCCCEEE	30.02	25106551
1057	Phosphorylation	FLLAGGRTKTWLVGN EEECCCCEEEEEECC	35.78	20068231
1058	Ubiquitination	LLAGGRTKTWLVGNK EECCCCEEEEEECCE	36.02	-
1068 (in isoform 2)	Ubiquitination	-	23.67	-
1076	Ubiquitination	VTTSVGTGTRSLLGL EEEECCCCHHHEECC	17.13	-
1079	Phosphorylation	SVGTGTRSLLGLDSG ECCCCHHHEECCCCC	27.99	23403867
1085	Phosphorylation	RSLLGLDSGELQSGP HHEECCCCCCCCCCC	38.93	23403867
1088	Ubiquitination	LGLDSGELQSGPESS ECCCCCCCCCCCCCC	5.83	-
1090	Phosphorylation	LDSGELQSGPESSSS CCCCCCCCCCCCCCC	69.78	28555341
1094	Phosphorylation	ELQSGPESSSSPGVH CCCCCCCCCCCCCCC	38.36	25159151
1095	Phosphorylation	LQSGPESSSSPGVHV CCCCCCCCCCCCCCC	32.92	23401153
1096	Phosphorylation	QSGPESSSSPGVHVR CCCCCCCCCCCCCCE	50.24	29255136
1097	Phosphorylation	SGPESSSSPGVHVRQ CCCCCCCCCCCCCEE	27.85	29255136
1106	Ubiquitination	GVHVRQTKEAPAKLE CCCCEECCCCCHHHH	43.47	-
1111	Ubiquitination	QTKEAPAKLESQAGQ ECCCCCHHHHHHHCC	52.29	-
1121	Phosphorylation	SQAGQQVSRGARDRV HHHCCHHHHHHHHHH	22.21	29507054
1122	Methylation	QAGQQVSRGARDRVR HHCCHHHHHHHHHHH	43.89	115919053
1130	Phosphorylation	GARDRVRSMSGGHGL HHHHHHHHCCCCCCC	17.91	23401153
1132	Phosphorylation	RDRVRSMSGGHGLRV HHHHHHCCCCCCCCC	42.75	29255136
1147	Phosphorylation	GALDVPASQFLGSAT CCEECCHHHHCCCCC	18.52	29396449
1150	Ubiquitination	DVPASQFLGSATSPG ECCHHHHCCCCCCCC	4.03	-
1152	Phosphorylation	PASQFLGSATSPGPR CHHHHCCCCCCCCCC	30.44	29255136
1154	Phosphorylation	SQFLGSATSPGPRTA HHHCCCCCCCCCCCC	36.83	29255136
1155	Phosphorylation	QFLGSATSPGPRTAP HHCCCCCCCCCCCCC	27.81	29255136
1159	Ubiquitination	SATSPGPRTAPAAKP CCCCCCCCCCCCCCC	49.81	-
1168	Ubiquitination	APAAKPEKASAGTRV CCCCCCCCCCCCCCC	57.05	-
1170	Ubiquitination	AAKPEKASAGTRVPV CCCCCCCCCCCCCCC	38.00	-
1177 (in isoform 7)	Phosphorylation	-	11.97	27251275
1179 (in isoform 7)	Phosphorylation	-	63.18	28348404
1180	Ubiquitination	TRVPVQEKTNLAAYV CCCCCCHHCCHHHHH	26.14	-
1181 (in isoform 7)	Phosphorylation	-	34.41	28348404
1186	Phosphorylation	EKTNLAAYVPLLTQG HHCCHHHHHHHHCCC	9.29	-
1189 (in isoform 6)	Phosphorylation	-	6.04	27251275
1189 (in isoform 7)	Phosphorylation	-	6.04	25159151
1191 (in isoform 6)	Phosphorylation	-	32.49	28348404
1193 (in isoform 6)	Phosphorylation	-	21.96	28348404
1201 (in isoform 6)	Phosphorylation	-	26.17	25159151
1217	Phosphorylation	MSLENPLSPFSSDIN EEECCCCCCCCCCCC	25.75	-
1225 (in isoform 5)	Phosphorylation	-	33.51	27251275
1227 (in isoform 5)	Phosphorylation	-	32.77	28348404
1229 (in isoform 5)	Phosphorylation	-	43.65	28348404
1237 (in isoform 5)	Phosphorylation	-	13.66	25159151
1242	Ubiquitination	LMAAERFKEHRDTAL HHHHHHHHHHHCCHH	59.85	-
1250	Phosphorylation	EHRDTALYKSLSVPA HHHCCHHHHHCCCCC	8.96	-
1251	Ubiquitination	HRDTALYKSLSVPAA HHCCHHHHHCCCCCC	45.85	-
1252	Phosphorylation	RDTALYKSLSVPAAS HCCHHHHHCCCCCCC	16.60	30631047
1254	Phosphorylation	TALYKSLSVPAASTA CHHHHHCCCCCCCCC	32.90	25159151
1259	Phosphorylation	SLSVPAASTAKPPPL HCCCCCCCCCCCCCC	30.88	20363803
1260	Phosphorylation	LSVPAASTAKPPPLP CCCCCCCCCCCCCCC	33.69	20068231
1262	Ubiquitination	VPAASTAKPPPLPRS CCCCCCCCCCCCCCC	59.13	-
1269	Phosphorylation	KPPPLPRSNTVASFS CCCCCCCCCCCCHHH	34.61	27251275
1269 (in isoform 4)	Phosphorylation	-	34.61	27251275
1271	Phosphorylation	PPLPRSNTVASFSSL CCCCCCCCCCHHHHH	20.78	28857561
1271 (in isoform 4)	Phosphorylation	-	20.78	28348404
1273 (in isoform 4)	Phosphorylation	-	11.65	28348404
1274	Phosphorylation	PRSNTVASFSSLYQS CCCCCCCHHHHHHHH	22.71	18669648
1281 (in isoform 4)	Phosphorylation	-	21.37	25159151
1290	Phosphorylation	CQGQLHRSVSWADSA CCCCCCCCCCHHCEE	15.12	23401153
1304	Phosphorylation	AVVMEEGSPGEVPVL EEEECCCCCCCCCEE	32.77	24275569
1330	Phosphorylation	ALGMDRRTDAYSRSS HHCCCCCCCCCCCCC	26.35	29083192
1333	Phosphorylation	MDRRTDAYSRSSSVS CCCCCCCCCCCCCCC	13.87	27642862
1334	Phosphorylation	DRRTDAYSRSSSVSS CCCCCCCCCCCCCCC	27.66	30576142
1336	Phosphorylation	RTDAYSRSSSVSSQE CCCCCCCCCCCCCHH	22.48	26699800
1337	Phosphorylation	TDAYSRSSSVSSQEE CCCCCCCCCCCCHHH	33.38	27794612
1338	Phosphorylation	DAYSRSSSVSSQEEK CCCCCCCCCCCHHHH	27.54	27794612
1340	Phosphorylation	YSRSSSVSSQEEKSL CCCCCCCCCHHHHHH	28.40	30576142
1341	Phosphorylation	SRSSSVSSQEEKSLH CCCCCCCCHHHHHHC	39.18	23911959
1346	Phosphorylation	VSSQEEKSLHAEELV CCCHHHHHHCHHHHH	28.77	29255136
1355	Methylation	HAEELVGRGIPIERV CHHHHHCCCCCCEEE	32.18	115919057
1364	Phosphorylation	IPIERVVSSEGGRPS CCCEEEECCCCCCCC	21.58	27273156
1365	Phosphorylation	PIERVVSSEGGRPSV CCEEEECCCCCCCCE	28.75	21712546
1371	Phosphorylation	SSEGGRPSVDLSFQP CCCCCCCCEECEECC	27.23	25159151
1375	Phosphorylation	GRPSVDLSFQPSQPL CCCCEECEECCCCCC	19.85	25159151
1379	Phosphorylation	VDLSFQPSQPLSKSS EECEECCCCCCCCCC	33.38	26074081
1383	Phosphorylation	FQPSQPLSKSSSSPE ECCCCCCCCCCCCHH	36.86	30576142
1385	Phosphorylation	PSQPLSKSSSSPELQ CCCCCCCCCCCHHHH	31.88	23927012
1386	Phosphorylation	SQPLSKSSSSPELQT CCCCCCCCCCHHHHH	38.27	25159151
1387	Phosphorylation	QPLSKSSSSPELQTL CCCCCCCCCHHHHHH	57.93	23927012
1388	Phosphorylation	PLSKSSSSPELQTLQ CCCCCCCCHHHHHHH	25.09	25159151
1393	Phosphorylation	SSSPELQTLQDILGD CCCHHHHHHHHHHCC	38.72	30278072
1411	Phosphorylation	KADVGRLSPEVKARS CCCHHCCCHHHHHHC	20.04	29255136
1418	Phosphorylation	SPEVKARSQSGTLDG CHHHHHHCCCCCCCC	33.29	30266825
1420	Phosphorylation	EVKARSQSGTLDGES HHHHHCCCCCCCCCC	34.68	23927012
1422	Phosphorylation	KARSQSGTLDGESAA HHHCCCCCCCCCCCC	27.24	30266825
1427	Phosphorylation	SGTLDGESAAWSASG CCCCCCCCCCCCCCC	28.90	23927012
1431	Phosphorylation	DGESAAWSASGEDSR CCCCCCCCCCCCCCC	14.08	23403867
1433	Phosphorylation	ESAAWSASGEDSRGQ CCCCCCCCCCCCCCC	36.88	27486199
1437	Phosphorylation	WSASGEDSRGQPEGP CCCCCCCCCCCCCCC	33.73	26074081
1447	Phosphorylation	QPEGPLPSSSPRSPS CCCCCCCCCCCCCCC	51.65	23401153
1448	Phosphorylation	PEGPLPSSSPRSPSG CCCCCCCCCCCCCCC	42.28	25159151
1449	Phosphorylation	EGPLPSSSPRSPSGL CCCCCCCCCCCCCCC	28.77	25159151
1452	Phosphorylation	LPSSSPRSPSGLRPR CCCCCCCCCCCCCCC	27.14	25159151
1454	Phosphorylation	SSSPRSPSGLRPRGY CCCCCCCCCCCCCCE	52.27	30266825
1461	Phosphorylation	SGLRPRGYTISDSAP CCCCCCCEECCCCCC	11.37	29255136
1462	Phosphorylation	GLRPRGYTISDSAPS CCCCCCEECCCCCCC	19.48	19664994
1464	Phosphorylation	RPRGYTISDSAPSRR CCCCEECCCCCCCCC	20.08	23403867
1466	Phosphorylation	RGYTISDSAPSRRGK CCEECCCCCCCCCCC	34.98	23403867
1469	Phosphorylation	TISDSAPSRRGKRVE ECCCCCCCCCCCCCH	34.03	-
1470	Ubiquitination	ISDSAPSRRGKRVER CCCCCCCCCCCCCHH	50.76	-
1539	Phosphorylation	QLLDQIPSYDTHKIA HHHHCCCCCCCCEEE	36.95	28348404
1542	Phosphorylation	DQIPSYDTHKIAVLY HCCCCCCCCEEEEEE	19.48	28348404
1543	Ubiquitination	QIPSYDTHKIAVLYV CCCCCCCCEEEEEEE	19.34	-
1571	Phosphorylation	NEHGSYRYTEFLTGL CCCCCHHHHHHHHHC	11.46	11290735
1574	Ubiquitination	GSYRYTEFLTGLGRL CCHHHHHHHHHCCCE	5.96	-
1585	Ubiquitination	LGRLIELKDCQPDKV CCCEEECCCCCCCCE	43.60	-
1586	Ubiquitination	GRLIELKDCQPDKVY CCEEECCCCCCCCEE	49.24	-
1624	Ubiquitination	AVFHIATLMPTKDVD HHHHHHHHCCCCCCC	2.60	-
1647	Phosphorylation	HLGNDFVSIVYNDSG CCCCCCEEEEECCCC	13.31	-
1650	Phosphorylation	NDFVSIVYNDSGEDF CCCEEEEECCCCCCE	16.16	-
1653	Phosphorylation	VSIVYNDSGEDFKLG EEEEECCCCCCEEEE	40.21	-
1658	Ubiquitination	NDSGEDFKLGTIKGQ CCCCCCEEEEEEEEC	59.33	-
1660	Ubiquitination	SGEDFKLGTIKGQFN CCCCEEEEEEEECEE	26.45	-
1684	Phosphorylation	DYECNLVSLQCRKDM CCCCCEEEEECCCCC	18.85	-
1689	Ubiquitination	LVSLQCRKDMEGLVD EEEEECCCCCCCCCC	69.88	-
1697	Phosphorylation	DMEGLVDTSVAKIVS CCCCCCCHHHHHHHC	20.02	-
1701	Ubiquitination	LVDTSVAKIVSDRNL CCCHHHHHHHCCCCC	41.29	-
1704	Phosphorylation	TSVAKIVSDRNLPFV HHHHHHHCCCCCHHH	34.03	-
1730	Phosphorylation	SQVHHSRSNPTDIYP HHCCCCCCCCCCCCC	50.61	27251275
1736	Phosphorylation	RSNPTDIYPSKWIAR CCCCCCCCCHHHHHH	12.31	25147952
1739	Ubiquitination	PTDIYPSKWIARLRH CCCCCCHHHHHHHHH	37.81	-
1760	Phosphorylation	RICEEAAYSNPSLPL HHHHHHHHCCCCCCC	18.94	21945579
1761	Phosphorylation	ICEEAAYSNPSLPLV HHHHHHHCCCCCCCC	37.22	21945579
1764	Phosphorylation	EAAYSNPSLPLVHPP HHHHCCCCCCCCCCC	47.04	21945579
1772	Phosphorylation	LPLVHPPSHSKAPAQ CCCCCCCCCCCCCCC	44.86	21712546
1774	Phosphorylation	LVHPPSHSKAPAQTP CCCCCCCCCCCCCCC	34.55	21712546
1775	Ubiquitination	VHPPSHSKAPAQTPA CCCCCCCCCCCCCCC	53.63	-
1780	Phosphorylation	HSKAPAQTPAEPTPG CCCCCCCCCCCCCCC	26.94	21945579
1785	Phosphorylation	AQTPAEPTPGYEVGQ CCCCCCCCCCCCHHH	22.35	21945579
1788	Phosphorylation	PAEPTPGYEVGQRKR CCCCCCCCCHHHCHH	14.51	21945579
1798	Phosphorylation	GQRKRLISSVEDFTE HHCHHHHCCHHHHHH	32.68	27273156
1799	Phosphorylation	QRKRLISSVEDFTEF HCHHHHCCHHHHHHC	23.64	25159151
1804	Phosphorylation	ISSVEDFTEFV---- HCCHHHHHHCC----	40.61	23403867

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
664	S	Phosphorylation	Kinase	ERK-SUBFAMILY	-	GPS
664	S	Phosphorylation	Kinase	MAPK3	P27361	GPS
664	S	Phosphorylation	Kinase	MAPK1	P28482	GPS
932	S	Phosphorylation	Kinase	PKCD	Q05655	PSP
939	S	Phosphorylation	Kinase	AKT1	P31749	Uniprot
939	S	Phosphorylation	Kinase	PKB_GROUP	-	PhosphoELM
939	S	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
939	S	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
939	S	Phosphorylation	Kinase	PKCD	Q05655	PSP
939	S	Phosphorylation	Kinase	SGK1	O00141	PSP
981	S	Phosphorylation	Kinase	SGK1	O00141	PSP
981	S	Phosphorylation	Kinase	AKT1	P31749	PSP
1130	S	Phosphorylation	Kinase	SGK1	O00141	PSP
1132	S	Phosphorylation	Kinase	SGK1	O00141	PSP
1217	S	Phosphorylation	Kinase	CDK6	Q00534	PSP
1217	S	Phosphorylation	Kinase	CDK4	P11802	PSP
1254	S	Phosphorylation	Kinase	MAPK2	P49137	PhosphoELM
1271	T	Phosphorylation	Kinase	AMPK	Q9Y478	Uniprot
1330	T	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
1387	S	Phosphorylation	Kinase	AMPK	Q9Y478	Uniprot
1387	S	Phosphorylation	Kinase	BRSK2	Q8IWQ3	PSP
1448	S	Phosphorylation	Kinase	AMPK-FAMILY	-	GPS
1452	S	Phosphorylation	Kinase	CDK6	Q00534	PSP
1452	S	Phosphorylation	Kinase	CDK4	P11802	PSP
1462	T	Phosphorylation	Kinase	SGK1	O00141	PSP
1462	T	Phosphorylation	Kinase	RPS6KA1	Q15418	GPS
1462	T	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
1462	T	Phosphorylation	Kinase	AKT1	P31749	Uniprot
1462	T	Phosphorylation	Kinase	PKB_GROUP	-	PhosphoELM
1798	S	Phosphorylation	Kinase	SGK1	O00141	PSP
1798	S	Phosphorylation	Kinase	P90RSK	Q15418	PSP
1798	S	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
1798	S	Phosphorylation	Kinase	RSK-SUBFAMILY	-	GPS
1798	S	Phosphorylation	Kinase	RSK_GROUP	-	PhosphoELM
-	K	Ubiquitination	E3 ubiquitin ligase	UBE3A	Q05086	PMID:18298802
-	K	Ubiquitination	E3 ubiquitin ligase	FBXW5	Q969U6	PMID:18381890
-	K	Ubiquitination	E3 ubiquitin ligase	MYCBP2	O75592	PMID:14559897
-	K	Ubiquitination	E3 ubiquitin ligase	HERC1	Q15751	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
939	S	Phosphorylation	12150915
Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
1387	S	Phosphorylation	14651849
Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
1418	S	Phosphorylation	15963462
Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
1420	S	Phosphorylation	15963462
Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
1462	T	Phosphorylation	12150915
Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
1798	S	Phosphorylation	15342917
Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
69	Acetylation	61 (8)	R ⇒ Q;P;L	rs45502703	Obesity-related traits	23251661

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
1433Z_HUMAN	YWHAZ	physical	12176984
AKT1_HUMAN	AKT1	physical	12167664
AKT1_HUMAN	AKT1	physical	15342917
CAV1_HUMAN	CAV1	physical	12147258
FOXO1_HUMAN	FOXO1	physical	17077083
GSK3B_HUMAN	GSK3B	physical	12511557
AMD_HUMAN	PAM	physical	14559897
AAPK1_HUMAN	PRKAA1	physical	14651849
FAK1_HUMAN	PTK2	physical	17043358
RAB5A_HUMAN	RAB5A	physical	12147258
RAP1A_HUMAN	RAP1A	physical	12147258
RHEB_HUMAN	RHEB	physical	12869586
RHEB_HUMAN	RHEB	physical	12820960
RHEB_HUMAN	RHEB	physical	12771962
RHEB_HUMAN	RHEB	physical	17077083
RHEB_HUMAN	RHEB	physical	12842888
KS6A1_HUMAN	RPS6KA1	physical	15342917
TSC1_HUMAN	TSC1	physical	12167664
TSC1_HUMAN	TSC1	physical	11741833
TSC1_HUMAN	TSC1	physical	11290735
TSC1_HUMAN	TSC1	physical	9580671
TSC1_HUMAN	TSC1	physical	12176984
TSC1_HUMAN	TSC1	physical	15340059
TSC1_HUMAN	TSC1	physical	12511557
TSC1_HUMAN	TSC1	physical	15851026
TSC1_HUMAN	TSC1	physical	16636147
TSC1_HUMAN	TSC1	physical	17077083
AXIN1_HUMAN	AXIN1	physical	12511557
UBE3A_HUMAN	UBE3A	physical	18298802
TSC1_HUMAN	TSC1	physical	17658474
TBCD7_HUMAN	TBC1D7	physical	17658474
1433B_HUMAN	YWHAB	physical	12582162
1433B_HUMAN	YWHAB	physical	12468542
1433B_HUMAN	YWHAB	physical	12364343
1433B_HUMAN	YWHAB	physical	12176984
HERC1_HUMAN	HERC1	physical	16464865
TSC1_HUMAN	TSC1	physical	16464865
ESR1_HUMAN	ESR1	physical	15039427
TSC1_HUMAN	TSC1	physical	15851513
SMAD2_HUMAN	SMAD2	physical	15066998
SMAD3_HUMAN	SMAD3	physical	15066998
1433T_HUMAN	YWHAQ	physical	20618440
MDFI_HUMAN	MDFI	physical	24722188
SPERT_HUMAN	SPERT	physical	24722188
TSC1_HUMAN	TSC1	physical	20166753
SIR1_HUMAN	SIRT1	physical	20169165
RRAGA_HUMAN	RRAGA	physical	24529380
RRAGB_HUMAN	RRAGB	physical	24529380
RRAGC_HUMAN	RRAGC	physical	24529380
RRAGD_HUMAN	RRAGD	physical	24529380
CBY1_HUMAN	CBY1	physical	27173435
RUVB2_HUMAN	RUVBL2	physical	28561026
TBCD7_HUMAN	TBC1D7	physical	28561026
TSC1_HUMAN	TSC1	physical	28561026
TSC2_HUMAN	TSC2	physical	28561026
1433G_HUMAN	YWHAG	physical	28561026
MCE1_HUMAN	RNGTT	genetic	26350902
CCNT1_HUMAN	CCNT1	genetic	26350902
CD11A_HUMAN	CDK11A	genetic	26350902

Drug and Disease Associations

Kegg Disease
H00896	Lymphangioleiomyomatosis (LAM)
H00915	Tuberous sclerosis complex (TSC); Bourneville-Pringle disease
OMIM Disease
613254	Tuberous sclerosis 2 (TSC2)
606690	Lymphangioleiomyomatosis (LAM)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of TSC2_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1155; SER-1449; THR-1462AND SER-1798, AND MASS SPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1388 AND SER-1420, ANDMASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-927; SER-981; SER-1132;SER-1155; SER-1334; SER-1337; SER-1338; SER-1341; SER-1411; SER-1420;SER-1449 AND SER-1452, AND MASS SPECTROMETRY.	18669648 [Abstract]
"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle."; Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.; Mol. Cell 31:438-448(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1155 AND SER-1411, ANDMASS SPECTROMETRY.	18691976 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1097 AND SER-1155, ANDMASS SPECTROMETRY.	18220336 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1411 AND SER-1798, ANDMASS SPECTROMETRY.	17081983 [Abstract]
"Phosphorylation and binding partner analysis of the TSC1-TSC2complex."; Nellist M., Burgers P.C., van den Ouweland A.M.W., Halley D.J.J.,Luider T.M.; Biochem. Biophys. Res. Commun. 333:818-826(2005). Cited for: PHOSPHORYLATION AT SER-1387; SER-1418 AND SER-1420, MASS SPECTROMETRY,INTERACTION WITH HSPA1; HSPA8 AND TSC1, AND CHARACTERIZATION OFVARIANTS TSC2 TRP-611 AND GLN-611.	15963462 [Abstract]
"Tumor-promoting phorbol esters and activated Ras inactivate thetuberous sclerosis tumor suppressor complex via p90 ribosomal S6kinase."; Roux P.P., Ballif B.A., Anjum R., Gygi S.P., Blenis J.; Proc. Natl. Acad. Sci. U.S.A. 101:13489-13494(2004). Cited for: PHOSPHORYLATION AT SER-1798.	15342917 [Abstract]
"TSC2 mediates cellular energy response to control cell growth andsurvival."; Inoki K., Zhu T., Guan K.L.; Cell 115:577-590(2003). Cited for: PHOSPHORYLATION AT THR-1330 AND SER-1448, AND MUTAGENESIS OF THR-1330AND SER-1448.	14651849 [Abstract]
"Identification of the tuberous sclerosis complex-2 tumor suppressorgene product tuberin as a target of the phosphoinositide 3-kinase/aktpathway."; Manning B.D., Tee A.R., Logsdon M.N., Blenis J., Cantley L.C.; Mol. Cell 10:151-162(2002). Cited for: PHOSPHORYLATION AT SER-939 AND THR-1462.	12150915 [Abstract]

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