RRAGA_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: RRAGA_HUMAN
• UniProt AC: Q7L523
• Protein Name: Ras-related GTP-binding protein A {ECO:0000305}
• Gene Name: RRAGA {ECO:0000312|HGNC:HGNC:16963}
• Organism: Homo sapiens (Human).
• Sequence Length: 313
• Subcellular Localization: Cytoplasm . Nucleus . Lysosome . Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state (PubMed:8995684, PubMed:9394008). Colocalizes in vivo with adenovirus E3-14.7K mainly to the cytoplasm
• Protein Description: Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade. Forms heterodimeric Rag complexes with RRAGC or RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form. In its active form participates in the relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. Involved in the RCC1/Ran-GTPase pathway. May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death. May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death..
• Protein Sequence: MPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKHSLLMR

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
15	Ubiquitination	KKVLLMGKSGSGKTS CEEEEECCCCCCCHH	37.36	27667366
16	Phosphorylation	KVLLMGKSGSGKTSM EEEEECCCCCCCHHH	32.25	20068231
18	Phosphorylation	LLMGKSGSGKTSMRS EEECCCCCCCHHHHH	44.18	20068231
21	Phosphorylation	GKSGSGKTSMRSIIF CCCCCCCHHHHHHHH	31.14	20068231
22	Phosphorylation	KSGSGKTSMRSIIFA CCCCCCHHHHHHHHH	18.71	20068231
25	Phosphorylation	SGKTSMRSIIFANYI CCCHHHHHHHHHHHH	16.05	20068231
31	Phosphorylation	RSIIFANYIARDTRR HHHHHHHHHHCCHHC	7.79	20068231
36	Phosphorylation	ANYIARDTRRLGATI HHHHHCCHHCCCCEE	16.49	24719451
42	Phosphorylation	DTRRLGATIDVEHSH CHHCCCCEEECCCHH	18.86	23312004
96	Phosphorylation	IYVFDVESRELEKDM EEEEECCCCHHHHHH	30.79	-
128	Ubiquitination	KIFCLVHKMDLVQED EEEEEEECHHHHCHH	26.99	29967540
142	Ubiquitination	DQRDLIFKEREEDLR HHHHHHHHHHHHHHH	49.20	27667366
152	Phosphorylation	EEDLRRLSRPLECAC HHHHHHHCCCHHHHH	30.00	29449344
215	Phosphorylation	RATFLVISHYQCKEQ HCCEEEEECCCCHHH	14.52	29083192
217	Phosphorylation	TFLVISHYQCKEQRD CEEEEECCCCHHHCC	14.60	29083192
220	Ubiquitination	VISHYQCKEQRDVHR EEECCCCHHHCCHHH	41.16	PubMed
230	Ubiquitination	RDVHRFEKISNIIKQ CCHHHHHHHHHHHHH	49.04	PubMed
232	Phosphorylation	VHRFEKISNIIKQFK HHHHHHHHHHHHHHH	32.80	28060719
236	Ubiquitination	EKISNIIKQFKLSCS HHHHHHHHHHHHCHH	45.98	33845483
239	Ubiquitination	SNIIKQFKLSCSKLA HHHHHHHHHCHHHHH	36.68	23000965
244	Ubiquitination	QFKLSCSKLAASFQS HHHHCHHHHHHHHHC	46.61	23000965
248	Phosphorylation	SCSKLAASFQSMEVR CHHHHHHHHHCCHHC	20.43	21406692
251	Phosphorylation	KLAASFQSMEVRNSN HHHHHHHCCHHCCCC	18.20	21406692
299	Ubiquitination	NARKHFEKLERVDGP HHHHHHHHHHCCCCC	55.49	29967540
307	Ubiquitination	LERVDGPKHSLLMR- HHCCCCCCCCCCCC-	51.89	33845483
307	Acetylation	LERVDGPKHSLLMR- HHCCCCCCCCCCCC-	51.89	21339330
309	Phosphorylation	RVDGPKHSLLMR--- CCCCCCCCCCCC---	29.35	29514088

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	RNF152	Q8N8N0	PMID:25936802
-	K	Ubiquitination	E3 ubiquitin ligase	SKP2	Q13309	PMID:26051179

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of RRAGA_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of RRAGA_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RRAGC_HUMAN	RRAGC	physical	16189514
NOL8_HUMAN	NOL8	physical	14660641
RRAGC_HUMAN	RRAGC	physical	11073942
RRAGD_HUMAN	RRAGD	physical	11073942
RRAGC_HUMAN	RRAGC	physical	21816923
RPTOR_HUMAN	RPTOR	physical	21816923
LTOR1_HUMAN	LAMTOR1	physical	21816923
RPTOR_HUMAN	RPTOR	physical	18497260
IL7RA_HUMAN	IL7R	physical	23151878
RRAGC_HUMAN	RRAGC	physical	23184942
RPTOR_HUMAN	RPTOR	physical	23184942
RRAGC_HUMAN	RRAGC	physical	24337580
RPTOR_HUMAN	RPTOR	physical	24337580
TSC2_HUMAN	TSC2	physical	24529380
TSC1_HUMAN	TSC1	physical	24529380
RRAGD_HUMAN	RRAGD	physical	26186194
RRAGC_HUMAN	RRAGC	physical	26186194
NPC2_HUMAN	NPC2	physical	26186194
CTU2_HUMAN	CTU2	physical	26186194
PPR1A_HUMAN	PPP1R1A	physical	26186194
RPTOR_HUMAN	RPTOR	physical	26186194
S38A9_HUMAN	SLC38A9	physical	26186194
RN152_HUMAN	RNF152	physical	25936802
RRAGC_HUMAN	RRAGC	physical	25936802
NPRL2_HUMAN	NPRL2	physical	25936802
NPRL3_HUMAN	NPRL3	physical	25936802
DEPD5_HUMAN	DEPDC5	physical	25936802
TSC2_HUMAN	TSC2	physical	25936802
RRAGD_HUMAN	RRAGD	physical	28514442
RRAGC_HUMAN	RRAGC	physical	28514442
S38A9_HUMAN	SLC38A9	physical	28514442
PPR1A_HUMAN	PPP1R1A	physical	28514442
RPTOR_HUMAN	RPTOR	physical	28514442
NPC2_HUMAN	NPC2	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.