NPRL2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NPRL2_HUMAN
• UniProt AC: Q8WTW4
• Protein Name: GATOR complex protein NPRL2 {ECO:0000305}
• Gene Name: NPRL2 {ECO:0000312|HGNC:HGNC:24969}
• Organism: Homo sapiens (Human).
• Sequence Length: 380
• Subcellular Localization: Lysosome membrane . Localization to lysosomes is amino acid-independent.
• Protein Description: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.; Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues. May act as a tumor suppressor. Suppresses cell growth and enhances sensitivity to various anticancer drugs..
• Protein Sequence: MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGRHPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGMSYHELDERLENDPNIIICWK

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
59	Ubiquitination	NKLITVTAMEKKLIG CCEEEEECCHHHHHC	10.37	22817900
59 (in isoform 2)	Ubiquitination	-	10.37	21906983
61	Ubiquitination	LITVTAMEKKLIGCP EEEEECCHHHHHCCC	44.57	22817900
62 (in isoform 1)	Ubiquitination	-	39.53	21906983
62	Ubiquitination	ITVTAMEKKLIGCPV EEEECCHHHHHCCCE	39.53	22817900
63	Ubiquitination	TVTAMEKKLIGCPVC EEECCHHHHHCCCEE	31.84	22817900
74	Ubiquitination	CPVCIEHKKYSRNAL CCEECCCCCCCCCCE	40.39	29967540
94	Ubiquitination	FVCDAQAKTCALEPI EECCHHHCHHCHHHH	31.86	-
100	Ubiquitination	AKTCALEPIVKKLAG HCHHCHHHHHHHHHH	37.21	21963094
101	Ubiquitination	KTCALEPIVKKLAGY CHHCHHHHHHHHHHH	5.32	22817900
103	Ubiquitination	CALEPIVKKLAGYLT HCHHHHHHHHHHHHH	42.67	21963094
104	Ubiquitination	ALEPIVKKLAGYLTT CHHHHHHHHHHHHHH	32.45	22817900
110	Phosphorylation	KKLAGYLTTLELESS HHHHHHHHHHHHHHH	22.35	22210691
111	Phosphorylation	KLAGYLTTLELESSF HHHHHHHHHHHHHHC	18.68	22210691
117	Phosphorylation	TTLELESSFVSMEES HHHHHHHHCCCHHHH	21.94	22210691
120	Phosphorylation	ELESSFVSMEESKQK HHHHHCCCHHHHHHH	20.61	-
138	Ubiquitination	IMTILLEELNASGRC HHHHHHHHHCCCCCC	47.49	21963094
158	Ubiquitination	ESNTIHLKVIEQRPD CCCEEEEEEEECCCC	27.31	-
176	Ubiquitination	AQEYDVPVFTKDKED CCCCCCCCEECCHHH	10.79	22817900
178	Ubiquitination	EYDVPVFTKDKEDFF CCCCCCEECCHHHHH	38.27	22817900
179 (in isoform 1)	Ubiquitination	-	56.17	21906983
179	Ubiquitination	YDVPVFTKDKEDFFN CCCCCEECCHHHHHH	56.17	22817900
181	Ubiquitination	VPVFTKDKEDFFNSQ CCCEECCHHHHHHCC	61.82	22817900
200	Ubiquitination	TQQILPYIDGFRHIQ HHHHHHHHCCCCCHH	3.84	21963094
237	Ubiquitination	YGVVTLVSILQYSNV HHHHHHHHHHHHCCC	22.24	21963094
255	Ubiquitination	TPKVQDLVDDKSLQE CCCHHHHCCCHHHHH	13.89	21963094
258	Ubiquitination	VQDLVDDKSLQEACL HHHHCCCHHHHHHHH	48.91	21963094
270	Ubiquitination	ACLSYVTKQGHKRAS HHHHHHHHHCCCCCC	44.21	29967540
277	Phosphorylation	KQGHKRASLRDVFQL HHCCCCCCHHHHHHH	28.15	32142685
287	Phosphorylation	DVFQLYCSLSPGTTV HHHHHHHCCCCCCCH	20.51	22210691
289	Phosphorylation	FQLYCSLSPGTTVRD HHHHHCCCCCCCHHH	12.65	22210691
292	Phosphorylation	YCSLSPGTTVRDLIG HHCCCCCCCHHHHHC	26.04	22210691
293	Phosphorylation	CSLSPGTTVRDLIGR HCCCCCCCHHHHHCC	21.32	22210691
312	Ubiquitination	LQHVDERKLIQFGLM HCCCCHHHHHHHHHH	48.14	-
317	Ubiquitination	ERKLIQFGLMKNLIR HHHHHHHHHHHHHHH	14.42	21963094
320	Ubiquitination	LIQFGLMKNLIRRLQ HHHHHHHHHHHHHHH	54.36	21963094
328	Ubiquitination	NLIRRLQKYPVRVTR HHHHHHHHCCCCCCH	56.99	-
345	Phosphorylation	QSHPARLYTGCHSYD HCCCCEEECCCCCHH	8.78	29496907
350	Phosphorylation	RLYTGCHSYDEICCK EEECCCCCHHHHHHC	38.05	29496907
354	Ubiquitination	GCHSYDEICCKTGMS CCCCHHHHHHCCCCC	2.82	21963094
357	Ubiquitination	SYDEICCKTGMSYHE CHHHHHHCCCCCHHH	43.64	21963094

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NPRL2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NPRL2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NPRL2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
KAP0_HUMAN	PRKAR1A	physical	17353931
UBC12_HUMAN	UBE2M	physical	17353931
NPRL3_HUMAN	NPRL3	physical	19521502
DEPD5_HUMAN	DEPDC5	physical	23723238
NPRL3_HUMAN	NPRL3	physical	23723238
WDR59_HUMAN	WDR59	physical	23723238
WDR24_HUMAN	WDR24	physical	23723238
SEH1_HUMAN	SEH1L	physical	23723238
HERC2_HUMAN	HERC2	physical	25480944
RRAGA_HUMAN	RRAGA	physical	25936802
DEPD5_HUMAN	DEPDC5	physical	25936802
DEPD5_HUMAN	DEPDC5	physical	28514442
NPRL3_HUMAN	NPRL3	physical	28514442
ZBTB5_HUMAN	ZBTB5	physical	28514442
APBP2_HUMAN	APPBP2	physical	28514442
LRCH2_HUMAN	LRCH2	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.