NPC2_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: NPC2_HUMAN
• UniProt AC: P61916
• Protein Name: NPC intracellular cholesterol transporter 2 {ECO:0000312|HGNC:HGNC:14537}
• Gene Name: NPC2 {ECO:0000312|HGNC:HGNC:14537}
• Organism: Homo sapiens (Human).
• Sequence Length: 151
• Subcellular Localization: Secreted . Endoplasmic reticulum . Lysosome .
• Protein Description: Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport..
• Protein Sequence: MRFLAATFLLLALSTAAQAEPVQFKDCGSVDGVIKEVNVSPCPTQPCQLSKGQSYSVNVTFTSNIQSKSSKAVVHGILMGVPVPFPIPEPDGCKSGINCPIQKDKTYSYLNKLPVKSEYPSIKLVVEWQLQDDKNQSLFCWEIPVQIVSHL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
7	Phosphorylation	-MRFLAATFLLLALS -CHHHHHHHHHHHHH	14.81	24043423
14	Phosphorylation	TFLLLALSTAAQAEP HHHHHHHHHHHHCCC	15.47	24043423
15	Phosphorylation	FLLLALSTAAQAEPV HHHHHHHHHHHCCCC	27.19	24043423
29	Phosphorylation	VQFKDCGSVDGVIKE CCCCCCCCCCCEEEE	24.30	21712546
44	O-linked_Glycosylation	VNVSPCPTQPCQLSK EECCCCCCCCCCCCC	52.43	OGP
58	N-linked_Glycosylation	KGQSYSVNVTFTSNI CCCEEEEEEEEECCC	22.59	17018531
58	N-linked_Glycosylation	KGQSYSVNVTFTSNI CCCEEEEEEEEECCC	22.59	16399764
79	Sulfoxidation	AVVHGILMGVPVPFP EEEEEEECCCCCCCC	4.86	28465586
93	Glutathionylation	PIPEPDGCKSGINCP CCCCCCCCCCCCCCC	4.06	22555962
103	Acetylation	GINCPIQKDKTYSYL CCCCCCCCCCCHHHH	63.01	26051181
103	2-Hydroxyisobutyrylation	GINCPIQKDKTYSYL CCCCCCCCCCCHHHH	63.01	-
105	Ubiquitination	NCPIQKDKTYSYLNK CCCCCCCCCHHHHHC	57.29	-
105	2-Hydroxyisobutyrylation	NCPIQKDKTYSYLNK CCCCCCCCCHHHHHC	57.29	-
106	Phosphorylation	CPIQKDKTYSYLNKL CCCCCCCCHHHHHCC	28.03	29759185
107	Phosphorylation	PIQKDKTYSYLNKLP CCCCCCCHHHHHCCC	10.89	-
108	Phosphorylation	IQKDKTYSYLNKLPV CCCCCCHHHHHCCCC	28.51	29759185
109	Phosphorylation	QKDKTYSYLNKLPVK CCCCCHHHHHCCCCC	12.13	29759185
112	Ubiquitination	KTYSYLNKLPVKSEY CCHHHHHCCCCCCCC	52.12	-
112	2-Hydroxyisobutyrylation	KTYSYLNKLPVKSEY CCHHHHHCCCCCCCC	52.12	-
116	Acetylation	YLNKLPVKSEYPSIK HHHCCCCCCCCCCEE	35.31	-
117	Phosphorylation	LNKLPVKSEYPSIKL HHCCCCCCCCCCEEE	42.47	28102081
119	Phosphorylation	KLPVKSEYPSIKLVV CCCCCCCCCCEEEEE	14.90	28102081
121	Phosphorylation	PVKSEYPSIKLVVEW CCCCCCCCEEEEEEE	31.30	28102081
135	N-linked_Glycosylation	WQLQDDKNQSLFCWE EEECCCCCCEEEEEE	43.60	16399764
135	N-linked_Glycosylation	WQLQDDKNQSLFCWE EEECCCCCCEEEEEE	43.60	17018531

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of NPC2_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of NPC2_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of NPC2_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DHDDS_HUMAN	DHDDS	physical	15110773
NUD19_HUMAN	NUDT19	physical	22939629
HXK3_HUMAN	HK3	physical	28514442
NDKB_HUMAN	NME2	physical	28514442
CFAB_HUMAN	CFB	physical	28514442
TTHY_HUMAN	TTR	physical	28514442
IRGQ_HUMAN	IRGQ	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 607625: Niemann-Pick disease C2 (NPC2)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

N-linked Glycosylation

"Glycoproteomics analysis of human liver tissue by combination ofmultiple enzyme digestion and hydrazide chemistry.";
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
J. Proteome Res. 8:651-661(2009).
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-135, AND MASSSPECTROMETRY.
PubMed: 19159218