dbPTM

NDKB_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NDKB_HUMAN
UniProt AC	P22392
Protein Name	Nucleoside diphosphate kinase B
Gene Name	NME2
Organism	Homo sapiens (Human).
Sequence Length	152
Subcellular Localization	Cytoplasm . Nucleus . Cell projection, lamellipodium . Cell projection, ruffle . Isoform 3 is mainly cytoplasmic and isoform 1 and isoform 3 are excluded from the nucleolus. Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and la
Protein Description	Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically. [PubMed: 8392752 Exhibits histidine protein kinase activity.]
Protein Sequence	MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MANLERTFI ------CCCCEEEEE	29.34	-
6	Methylation	--MANLERTFIAIKP --CCCCEEEEEEECC	37.79	115485165
7	Phosphorylation	-MANLERTFIAIKPD -CCCCEEEEEEECCC	15.26	30624053
12	Acetylation	ERTFIAIKPDGVQRG EEEEEEECCCCHHHC	28.21	22638521
12	Ubiquitination	ERTFIAIKPDGVQRG EEEEEEECCCCHHHC	28.21	27667366
12 (in isoform 2)	Ubiquitination	-	28.21	21890473
12 (in isoform 1)	Ubiquitination	-	28.21	21890473
18	Methylation	IKPDGVQRGLVGEII ECCCCHHHCHHHHHH	37.70	-
26	Acetylation	GLVGEIIKRFEQKGF CHHHHHHHHHHHCCH	57.61	8275725
26	Ubiquitination	GLVGEIIKRFEQKGF CHHHHHHHHHHHCCH	57.61	23000965
26	Neddylation	GLVGEIIKRFEQKGF CHHHHHHHHHHHCCH	57.61	32015554
26 (in isoform 2)	Ubiquitination	-	57.61	21890473
26 (in isoform 1)	Ubiquitination	-	57.61	21890473
31	Acetylation	IIKRFEQKGFRLVAM HHHHHHHCCHHHHHH	53.47	129823
31	Ubiquitination	IIKRFEQKGFRLVAM HHHHHHHCCHHHHHH	53.47	23000965
31	Neddylation	IIKRFEQKGFRLVAM HHHHHHHCCHHHHHH	53.47	32015554
39	Acetylation	GFRLVAMKFLRASEE CHHHHHHHHHHHCHH	31.35	25825284
39	Ubiquitination	GFRLVAMKFLRASEE CHHHHHHHHHHHCHH	31.35	23000965
39 (in isoform 2)	Ubiquitination	-	31.35	21890473
39 (in isoform 1)	Ubiquitination	-	31.35	21890473
42	Methylation	LVAMKFLRASEEHLK HHHHHHHHHCHHHHH	38.92	115485181
44	Phosphorylation	AMKFLRASEEHLKQH HHHHHHHCHHHHHHH	36.76	22617229
49	Acetylation	RASEEHLKQHYIDLK HHCHHHHHHHCCCCC	36.62	23954790
49	Ubiquitination	RASEEHLKQHYIDLK HHCHHHHHHHCCCCC	36.62	23000965
49 (in isoform 2)	Ubiquitination	-	36.62	21890473
49 (in isoform 1)	Ubiquitination	-	36.62	21890473
52	Phosphorylation	EEHLKQHYIDLKDRP HHHHHHHCCCCCCCC	8.14	28152594
56	Acetylation	KQHYIDLKDRPFFPG HHHCCCCCCCCCCCC	48.20	22638529
56	Ubiquitination	KQHYIDLKDRPFFPG HHHCCCCCCCCCCCC	48.20	23000965
56	Neddylation	KQHYIDLKDRPFFPG HHHCCCCCCCCCCCC	48.20	32015554
56 (in isoform 2)	Ubiquitination	-	48.20	21890473
56 (in isoform 1)	Ubiquitination	-	48.20	21890473
58	Methylation	HYIDLKDRPFFPGLV HCCCCCCCCCCCCHH	28.47	115485173
66	Acetylation	PFFPGLVKYMNSGPV CCCCCHHHHCCCCCE	43.77	30585623
66	Ubiquitination	PFFPGLVKYMNSGPV CCCCCHHHHCCCCCE	43.77	-
67	Phosphorylation	FFPGLVKYMNSGPVV CCCCHHHHCCCCCEE	8.27	26330541
85	Acetylation	WEGLNVVKTGRVMLG ECCCCEEEECCEEEC	40.76	19608861
85	Ubiquitination	WEGLNVVKTGRVMLG ECCCCEEEECCEEEC	40.76	22817900
85 (in isoform 2)	Ubiquitination	-	40.76	21890473
85 (in isoform 1)	Ubiquitination	-	40.76	21890473
88	Methylation	LNVVKTGRVMLGETN CCEEEECCEEECCCC	18.86	-
90	Sulfoxidation	VVKTGRVMLGETNPA EEEECCEEECCCCCC	3.71	30846556
94	Phosphorylation	GRVMLGETNPADSKP CCEEECCCCCCCCCC	44.71	29255136
99	Phosphorylation	GETNPADSKPGTIRG CCCCCCCCCCCCCCC	42.74	30266825
100	Methylation	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	7618957
100	Acetylation	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	7618957
100	Ubiquitination	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	27667366
100	Sumoylation	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	-
100	Neddylation	ETNPADSKPGTIRGD CCCCCCCCCCCCCCC	48.27	32015554
100 (in isoform 2)	Ubiquitination	-	48.27	21890473
100 (in isoform 1)	Ubiquitination	-	48.27	21890473
103	Phosphorylation	PADSKPGTIRGDFCI CCCCCCCCCCCCEEE	18.94	23927012
109	S-palmitoylation	GTIRGDFCIQVGRNI CCCCCCEEEEECCCE	2.24	29575903
114	Methylation	DFCIQVGRNIIHGSD CEEEEECCCEEECCC	32.11	115485189
118	Phosphorylation	QVGRNIIHGSDSVKS EECCCEEECCCHHHC	26.38	-
120	Phosphorylation	GRNIIHGSDSVKSAE CCCEEECCCHHHCCH	16.73	29255136
122	Phosphorylation	NIIHGSDSVKSAEKE CEEECCCHHHCCHHE	32.89	29255136
124	Acetylation	IHGSDSVKSAEKEIS EECCCHHHCCHHEEE	48.15	22638497
124	Ubiquitination	IHGSDSVKSAEKEIS EECCCHHHCCHHEEE	48.15	23000965
124	Sumoylation	IHGSDSVKSAEKEIS EECCCHHHCCHHEEE	48.15	-
124	Neddylation	IHGSDSVKSAEKEIS EECCCHHHCCHHEEE	48.15	32015554
124 (in isoform 1)	Ubiquitination	-	48.15	21890473
125	Phosphorylation	HGSDSVKSAEKEISL ECCCHHHCCHHEEEC	39.23	26330541
127	Ubiquitination	SDSVKSAEKEISLWF CCHHHCCHHEEECEE	59.31	23000965
128	Acetylation	DSVKSAEKEISLWFK CHHHCCHHEEECEEC	61.13	23954790
128	Ubiquitination	DSVKSAEKEISLWFK CHHHCCHHEEECEEC	61.13	23000965
128 (in isoform 1)	Ubiquitination	-	61.13	21890473
131	Phosphorylation	KSAEKEISLWFKPEE HCCHHEEECEECHHH	22.19	-
135	Ubiquitination	KEISLWFKPEELVDY HEEECEECHHHHCCC	39.68	21906983
135	Acetylation	KEISLWFKPEELVDY HEEECEECHHHHCCC	39.68	26822725
135 (in isoform 1)	Ubiquitination	-	39.68	21890473
141	Acetylation	FKPEELVDYKSCAHD ECHHHHCCCCCCCCC	58.74	-
141	Ubiquitination	FKPEELVDYKSCAHD ECHHHHCCCCCCCCC	58.74	23000965
141	Neddylation	FKPEELVDYKSCAHD ECHHHHCCCCCCCCC	58.74	32015554
142	Phosphorylation	KPEELVDYKSCAHDW CHHHHCCCCCCCCCC	9.42	-
143	Acetylation	PEELVDYKSCAHDWV HHHHCCCCCCCCCCC	34.30	70615
143	Ubiquitination	PEELVDYKSCAHDWV HHHHCCCCCCCCCCC	34.30	29967540
144	Phosphorylation	EELVDYKSCAHDWVY HHHCCCCCCCCCCCC	15.42	27134283
145	Glutathionylation	ELVDYKSCAHDWVYE HHCCCCCCCCCCCCC	3.22	22555962
145	S-palmitoylation	ELVDYKSCAHDWVYE HHCCCCCCCCCCCCC	3.22	29575903
146	Ubiquitination	LVDYKSCAHDWVYE- HCCCCCCCCCCCCC-	15.68	23000965
146	Neddylation	LVDYKSCAHDWVYE- HCCCCCCCCCCCCC-	15.68	32015554
151	Phosphorylation	SCAHDWVYE------ CCCCCCCCC------	16.21	26356563
154	Ubiquitination	HDWVYE--------- CCCCCC---------		21890473
154 (in isoform 2)	Ubiquitination	-		21890473
159	Phosphorylation	E-------------- C--------------		8245015
164	Acetylation	------------------- -------------------		19608861
164	Ubiquitination	------------------- -------------------		23000965
164 (in isoform 2)	Ubiquitination	-		21890473
171	Acetylation	-------------------------- --------------------------		19608861
171	Ubiquitination	-------------------------- --------------------------		23000965
171	Neddylation	-------------------------- --------------------------		32015554
171 (in isoform 2)	Ubiquitination	-		21890473
181	Acetylation	------------------------------------ ------------------------------------		-
181	Ubiquitination	------------------------------------ ------------------------------------		-
200	Acetylation	------------------------------------------------------- -------------------------------------------------------		19608861
200	Ubiquitination	------------------------------------------------------- -------------------------------------------------------		22817900
200 (in isoform 2)	Ubiquitination	-		-
209	Phosphorylation	---------------------------------------------------------------- ----------------------------------------------------------------		32645325
214	Phosphorylation	--------------------------------------------------------------------- ---------------------------------------------------------------------		-
215	Methylation	---------------------------------------------------------------------- ----------------------------------------------------------------------		-
215	Acetylation	---------------------------------------------------------------------- ----------------------------------------------------------------------		-
215	Ubiquitination	---------------------------------------------------------------------- ----------------------------------------------------------------------		23000965
215	Neddylation	---------------------------------------------------------------------- ----------------------------------------------------------------------		32015554
218	Phosphorylation	------------------------------------------------------------------------- -------------------------------------------------------------------------		-
233	Phosphorylation	---------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------		1851158
235	Phosphorylation	------------------------------------------------------------------------------------------ ------------------------------------------------------------------------------------------		20068231
237	Phosphorylation	-------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------		20068231
239	Acetylation	---------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------		19608861
239	Ubiquitination	---------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------		23000965
239	Neddylation	---------------------------------------------------------------------------------------------- ----------------------------------------------------------------------------------------------		32015554
239 (in isoform 2)	Ubiquitination	-		21890473
243	Acetylation	-------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------		19608861
243	Ubiquitination	-------------------------------------------------------------------------------------------------- --------------------------------------------------------------------------------------------------		23000965
243 (in isoform 2)	Ubiquitination	-		21890473
250	Ubiquitination	--------------------------------------------------------------------------------------------------------- ---------------------------------------------------------------------------------------------------------		32015554
250 (in isoform 2)	Ubiquitination	-		21890473
258	Ubiquitination	----------------------------------------------------------------------------------------------------------------- -----------------------------------------------------------------------------------------------------------------		29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
94	T	Phosphorylation	Kinase	CDK1	P06493	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	HERC5	Q9UII4	PMID:18535780

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NDKB_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NDKB_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DYHC1_HUMAN	DYNC1H1	physical	17353931
PP1R7_HUMAN	PPP1R7	physical	17353931
EPIPL_HUMAN	EPPK1	physical	17353931
ACLY_HUMAN	ACLY	physical	17353931
LYSC_HUMAN	LYZ	physical	17353931
RPAB3_HUMAN	POLR2H	physical	17353931
5HT1B_HUMAN	HTR1B	physical	17353931
PP1R8_HUMAN	PPP1R8	physical	17353931
RUVB2_HUMAN	RUVBL2	physical	17353931
PDIA6_HUMAN	PDIA6	physical	17353931
PUR9_HUMAN	ATIC	physical	17353931
PSA2_HUMAN	PSMA2	physical	17353931
PSA7_HUMAN	PSMA7	physical	17353931
STIP1_HUMAN	STIP1	physical	17353931
KINH_HUMAN	KIF5B	physical	17353931
RL38_HUMAN	RPL38	physical	17353931
NDKB_HUMAN	NME2	physical	7658474
GBB1_RAT	Gnb1	physical	12486122
MDM2_HUMAN	MDM2	physical	21504894
RS3_HUMAN	RPS3	physical	22939629
SODC_HUMAN	SOD1	physical	22939629
NDKM_HUMAN	NME4	physical	21988832
RAB5A_HUMAN	RAB5A	physical	21988832
SAHH_HUMAN	AHCY	physical	26344197
AK1A1_HUMAN	AKR1A1	physical	26344197
ALDR_HUMAN	AKR1B1	physical	26344197
ENOA_HUMAN	ENO1	physical	26344197
ENOG_HUMAN	ENO2	physical	26344197
ENOB_HUMAN	ENO3	physical	26344197
FKB1A_HUMAN	FKBP1A	physical	26344197
FKB1B_HUMAN	FKBP1B	physical	26344197
FKBP2_HUMAN	FKBP2	physical	26344197
AATC_HUMAN	GOT1	physical	26344197
PRDX5_HUMAN	PRDX5	physical	26344197
STIP1_HUMAN	STIP1	physical	26344197
TAGL_HUMAN	TAGLN	physical	26344197
TAGL2_HUMAN	TAGLN2	physical	26344197
UB2V1_HUMAN	UBE2V1	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
There are no disease associations of PTM sites.
DrugBank
DB00718	Adefovir Dipivoxil
DB00709	Lamivudine
DB00300	Tenofovir

Regulatory Network of NDKB_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, AND MASS SPECTROMETRY.	19413330 [Abstract]
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-12; LYS-49; LYS-56; LYS-85;LYS-100; LYS-124 AND LYS-128, AND MASS SPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"An extensive survey of tyrosine phosphorylation revealing new sitesin human mammary epithelial cells."; Heibeck T.H., Ding S.-J., Opresko L.K., Zhao R., Schepmoes A.A.,Yang F., Tolmachev A.V., Monroe M.E., Camp D.G. II, Smith R.D.,Wiley H.S., Qian W.-J.; J. Proteome Res. 8:3852-3861(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-52, AND MASSSPECTROMETRY.	19534553 [Abstract]