| UniProt ID | AXIN1_HUMAN | |
|---|---|---|
| UniProt AC | O15169 | |
| Protein Name | Axin-1 | |
| Gene Name | AXIN1 | |
| Organism | Homo sapiens (Human). | |
| Sequence Length | 862 | |
| Subcellular Localization | Cytoplasm . Nucleus . Membrane . Cell membrane . MACF1 is required for its translocation to cell membrane (By similarity). On UV irradiation, translocates to the nucleus and colocalizes with DAAX (PubMed:17210684). | |
| Protein Description | Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. [PubMed: 12192039] | |
| Protein Sequence | MNIQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPASYSFCSGKGVGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACTGFRKLEPCDSNEEKRLKLARAIYRKYILDNNGIVSRQTKPATKSFIKGCIMKQLIDPAMFDQAQTEIQATMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGISGYLPTLNEDEEWKCDQDMDEDDGRDAAPPGRLPQKLLLETAAPRVSSSRRYSEGREFRYGSWREPVNPYYVNAGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRVPKEVRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGDPSSGPPGPCHKLPPAPAWHHFPPRCVDMGCAGLRDAHEENPESILDEHVQRVLRTPGRQSPGPGHRSPDSGHVAKMPVALGGAASGHGKHVPKSGAKLDAAGLHHHRHVHHHVHHSTARPKEQVEAEATRRAQSSFAWGLEPHSHGARSRGYSESVGAAPNASDGLAHSGKVGVACKRNAKKAESGKSASTEVPGASEDAEKNQKIMQWIIEGEKEISRHRRTGHGSSGTRKPQPHENSRPLSLEHPWAGPQLRTSVQPSHLFIQDPTMPPHPAPNPLTQLEEARRRLEEEEKRASRAPSKQRYVQEVMRRGRACVRPACAPVLHVVPAVSDMELSETETRSQRKVGGGSAQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVD | |
| Overview of Protein Modification Sites with Functional and Structural Information | ||
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* ASA = Accessible Surface Area
| Locations | Modification | Substrate Peptides & Secondary Structure |
ASA (%) | Reference | Orthologous Protein Cluster |
|---|---|---|---|---|---|
| 42 | Phosphorylation | TDPRPASYSFCSGKG CCCCCCCEEECCCCC | 14.10 | 27642862 | |
| 58 | Phosphorylation | GIKGETSTATPRRSD CCCCCCCCCCCCHHH | 41.80 | 23312004 | |
| 60 | Phosphorylation | KGETSTATPRRSDLD CCCCCCCCCCHHHCC | 19.88 | 28985074 | |
| 64 | Phosphorylation | STATPRRSDLDLGYE CCCCCCHHHCCCCCC | 43.75 | 23403867 | |
| 70 | Phosphorylation | RSDLDLGYEPEGSAS HHHCCCCCCCCCCCC | 35.29 | 23403867 | |
| 75 | Phosphorylation | LGYEPEGSASPTPPY CCCCCCCCCCCCCCH | 24.74 | 25159151 | |
| 77 | Phosphorylation | YEPEGSASPTPPYLK CCCCCCCCCCCCHHH | 30.88 | 25159151 | |
| 77 (in isoform 2) | Phosphorylation | - | 30.88 | - | |
| 79 | Phosphorylation | PEGSASPTPPYLKWA CCCCCCCCCCHHHHH | 34.11 | 25159151 | |
| 82 | Phosphorylation | SASPTPPYLKWAESL CCCCCCCHHHHHHHH | 23.32 | 28176443 | |
| 148 | Phosphorylation | ARAIYRKYILDNNGI HHHHHHHHHHHCCCC | 9.56 | 18491316 | |
| 148 (in isoform 2) | Phosphorylation | - | 9.56 | - | |
| 160 (in isoform 2) | Phosphorylation | - | 37.57 | - | |
| 160 | Phosphorylation | NGIVSRQTKPATKSF CCCCCCCCCHHCHHH | 37.57 | 18491316 | |
| 166 | Phosphorylation | QTKPATKSFIKGCIM CCCHHCHHHHHHHHH | 28.05 | 24719451 | |
| 200 | Phosphorylation | MEENTYPSFLKSDIY HHHCCCHHHHCCCEE | 32.56 | 24719451 | |
| 213 (in isoform 2) | Phosphorylation | - | 36.94 | - | |
| 213 | Phosphorylation | IYLEYTRTGSESPKV EEEEEEECCCCCCCE | 36.94 | 28450419 | |
| 215 | Phosphorylation | LEYTRTGSESPKVCS EEEEECCCCCCCEEC | 34.00 | 25849741 | |
| 217 (in isoform 2) | Phosphorylation | - | 31.55 | - | |
| 217 | Phosphorylation | YTRTGSESPKVCSDQ EEECCCCCCCEECCC | 31.55 | 23401153 | |
| 222 | Dephosphorylation | SESPKVCSDQSSGSG CCCCCEECCCCCCCC | 41.49 | 17318175 | |
| 222 | Phosphorylation | SESPKVCSDQSSGSG CCCCCEECCCCCCCC | 41.49 | 23403867 | |
| 225 | Phosphorylation | PKVCSDQSSGSGTGK CCEECCCCCCCCCCC | 41.48 | 23403867 | |
| 226 | Phosphorylation | KVCSDQSSGSGTGKG CEECCCCCCCCCCCC | 31.20 | 23403867 | |
| 228 | Phosphorylation | CSDQSSGSGTGKGIS ECCCCCCCCCCCCCC | 34.82 | 23403867 | |
| 230 | Phosphorylation | DQSSGSGTGKGISGY CCCCCCCCCCCCCCC | 37.10 | 23403867 | |
| 281 | Phosphorylation | ETAAPRVSSSRRYSE HHCCCCCCCCCCCCC | 24.71 | 26437602 | |
| 282 | Phosphorylation | TAAPRVSSSRRYSEG HCCCCCCCCCCCCCC | 25.63 | 20068231 | |
| 283 | Phosphorylation | AAPRVSSSRRYSEGR CCCCCCCCCCCCCCC | 17.17 | 20068231 | |
| 387 | Ubiquitination | EVRVEPQKFAEELIH CCCCCHHHHHHHHHH | 58.20 | - | |
| 469 | Phosphorylation | AHEENPESILDEHVQ HHHHCHHHHHHHHHH | 29.70 | 20873877 | |
| 481 | Phosphorylation | HVQRVLRTPGRQSPG HHHHHHCCCCCCCCC | 26.40 | 26055452 | |
| 486 | Phosphorylation | LRTPGRQSPGPGHRS HCCCCCCCCCCCCCC | 30.39 | 23401153 | |
| 493 (in isoform 2) | Phosphorylation | - | 27.64 | - | |
| 493 | Phosphorylation | SPGPGHRSPDSGHVA CCCCCCCCCCCCCCC | 27.64 | 23401153 | |
| 496 | Phosphorylation | PGHRSPDSGHVAKMP CCCCCCCCCCCCCCC | 33.89 | 20873877 | |
| 511 | Phosphorylation | VALGGAASGHGKHVP EEECCCCCCCCCCCC | 30.68 | 25159151 | |
| 542 | Phosphorylation | VHHHVHHSTARPKEQ CCCCCCCCCCCHHHH | 14.64 | 22798277 | |
| 560 | Phosphorylation | EATRRAQSSFAWGLE HHHHHHHHHHHHCCC | 26.83 | 28555341 | |
| 561 | Phosphorylation | ATRRAQSSFAWGLEP HHHHHHHHHHHCCCC | 13.60 | 20873877 | |
| 570 | Phosphorylation | AWGLEPHSHGARSRG HHCCCCCCCCHHHCC | 34.04 | 20873877 | |
| 575 | Phosphorylation | PHSHGARSRGYSESV CCCCCHHHCCCCCCC | 29.53 | 25159151 | |
| 576 | Methylation | HSHGARSRGYSESVG CCCCHHHCCCCCCCC | 42.38 | - | |
| 578 | Phosphorylation | HGARSRGYSESVGAA CCHHHCCCCCCCCCC | 14.25 | 29978859 | |
| 579 | Phosphorylation | GARSRGYSESVGAAP CHHHCCCCCCCCCCC | 26.64 | 29978859 | |
| 581 | Phosphorylation | RSRGYSESVGAAPNA HHCCCCCCCCCCCCC | 21.65 | 25159151 | |
| 589 | Phosphorylation | VGAAPNASDGLAHSG CCCCCCCCCCCCCCC | 38.56 | 23312004 | |
| 595 | Phosphorylation | ASDGLAHSGKVGVAC CCCCCCCCCCHHHHC | 34.40 | 23186163 | |
| 611 | Phosphorylation | RNAKKAESGKSASTE HCHHHCCCCCCCCCC | 57.28 | - | |
| 614 (in isoform 2) | Phosphorylation | - | 30.86 | - | |
| 614 | Phosphorylation | KKAESGKSASTEVPG HHCCCCCCCCCCCCC | 30.86 | 10581160 | |
| 616 | Phosphorylation | AESGKSASTEVPGAS CCCCCCCCCCCCCCC | 31.99 | - | |
| 617 | Phosphorylation | ESGKSASTEVPGASE CCCCCCCCCCCCCCC | 40.49 | - | |
| 623 | Phosphorylation | STEVPGASEDAEKNQ CCCCCCCCCCHHHHH | 41.76 | - | |
| 644 | Phosphorylation | IEGEKEISRHRRTGH HHCCHHHHCCCCCCC | 24.11 | 27251275 | |
| 665 | Phosphorylation | KPQPHENSRPLSLEH CCCCCCCCCCCCCCC | 32.08 | 25690035 | |
| 730 | Phosphorylation | RAPSKQRYVQEVMRR CCCHHHHHHHHHHHH | 12.18 | 24043423 | |
| 787 | Phosphorylation | CDSIVVAYYFCGEPI CCEEEEEEEECCCCC | 5.86 | 22817900 | |
| 788 | Phosphorylation | DSIVVAYYFCGEPIP CEEEEEEEECCCCCC | 5.22 | 22817900 | |
| 789 (in isoform 2) | Ubiquitination | - | 2.55 | - | |
| 796 | Phosphorylation | FCGEPIPYRTLVRGR ECCCCCCHHHHCCCC | 19.56 | 22817900 | |
| 806 | Phosphorylation | LVRGRAVTLGQFKEL HCCCCEEEHHHHHHH | 24.96 | 24043423 | |
| 821 (in isoform 2) | Ubiquitination | - | 22.75 | - | |
| 821 (in isoform 2) | Sumoylation | - | 22.75 | - | |
| 857 | Sumoylation | FEEKIIGKVEKVD-- HHHHHCCEEEECC-- | 36.49 | 12223491 | |
| 857 | Sumoylation | FEEKIIGKVEKVD-- HHHHHCCEEEECC-- | 36.49 | - | |
| 860 | Sumoylation | KIIGKVEKVD----- HHCCEEEECC----- | 55.48 | 12223491 | |
| 860 | Sumoylation | KIIGKVEKVD----- HHCCEEEECC----- | 55.48 | - |
| Modified Location | Modified Residue | Modification | Type of Upstream Proteins | Gene Name of Upstream Proteins | UniProt AC of Upstream Proteins | Sources |
|---|---|---|---|---|---|---|
| 75 | S | Phosphorylation | Kinase | CK1-FAMILY | - | GPS |
| 75 | S | Phosphorylation | Kinase | CK1 | - | Uniprot |
| 77 | S | Phosphorylation | Kinase | CK1-FAMILY | - | GPS |
| 77 | S | Phosphorylation | Kinase | CK1 | - | Uniprot |
| 217 | S | Phosphorylation | Kinase | CK1-FAMILY | - | GPS |
| 217 | S | Phosphorylation | Kinase | CK1 | - | Uniprot |
| 469 | S | Phosphorylation | Kinase | CK1-FAMILY | - | GPS |
| 469 | S | Phosphorylation | Kinase | CK1 | - | Uniprot |
| 481 | T | Phosphorylation | Kinase | CDK5 | Q00535 | PSP |
| 481 | T | Phosphorylation | Kinase | GSK3-BETA | P49841 | Uniprot |
| - | K | Ubiquitination | E3 ubiquitin ligase | SMURF2 | Q9HAU4 | PMID:20858899 |
| - | K | Ubiquitination | E3 ubiquitin ligase | SMURF1 | Q9HCE7 | PMID:23959799 |
| - | K | Ubiquitination | E3 ubiquitin ligase | RNF146 | Q9NTX7 | PMID:21478859 |
* Distance = the distance between SAP position and PTM sites.
| Modified Location | Modification | Variant Position (Distance <= 10) |
Residue Change | SAP | Related Disease | Reference |
|---|---|---|---|---|---|---|
Oops, there are no SNP-PTM records of AXIN1_HUMAN !! | ||||||
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| Phosphorylation | |
| Reference | PubMed |
| "Protein phosphatase 1 regulates assembly and function of the beta-catenin degradation complex."; Luo W., Peterson A., Garcia B.A., Coombs G., Kofahl B., Heinrich R.,Shabanowitz J., Hunt D.F., Yost H.J., Virshup D.M.; EMBO J. 26:1511-1521(2007). Cited for: PHOSPHORYLATION AT SER-75; SER-77; SER-217 AND SER-469, MASSSPECTROMETRY, AND INTERACTION WITH GSK3B AND PPP1CA. | |
