dbPTM

PUF60_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	PUF60_HUMAN
UniProt AC	Q9UHX1
Protein Name	Poly(U)-binding-splicing factor PUF60
Gene Name	PUF60
Organism	Homo sapiens (Human).
Sequence Length	559
Subcellular Localization	Nucleus . Colocalizes partially with TROVE2.
Protein Description	DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA..
Protein Sequence	MATATIALQVNGQQGGGSEPAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MATATIALQV -----CCEEEEEEEE	24.04	18491316
3 (in isoform 2)	Phosphorylation	-	24.04	-
3 (in isoform 5)	Phosphorylation	-	24.04	20860994
3 (in isoform 6)	Phosphorylation	-	24.04	20860994
5	Phosphorylation	---MATATIALQVNG ---CCEEEEEEEECC	11.48	18491316
5 (in isoform 5)	Phosphorylation	-	11.48	20860994
5 (in isoform 6)	Phosphorylation	-	11.48	20860994
10 (in isoform 5)	Phosphorylation	-	10.13	20860994
10 (in isoform 6)	Phosphorylation	-	10.13	20860994
12 (in isoform 5)	Phosphorylation	-	23.49	21406692
12 (in isoform 6)	Phosphorylation	-	23.49	21406692
14 (in isoform 5)	Sumoylation	-	54.63	-
14 (in isoform 6)	Sumoylation	-	54.63	-
18	Phosphorylation	NGQQGGGSEPAAAAA CCCCCCCCCHHHHHH	43.62	25867546
26	Ubiquitination	EPAAAAAVVAAGDKW CHHHHHHHHHCCCCC	2.30	29967540
29	Ubiquitination	AAAAVVAAGDKWKPP HHHHHHHCCCCCCCC	18.33	29967540
33	Ubiquitination	VVAAGDKWKPPQGTD HHHCCCCCCCCCCCC	24.11	29967540
34	Ubiquitination	VAAGDKWKPPQGTDS HHCCCCCCCCCCCCC	53.19	29967540
37	Ubiquitination	GDKWKPPQGTDSIKM CCCCCCCCCCCCEEE	75.77	23000965
37 (in isoform 3)	Ubiquitination	-	75.77	21890473
37 (in isoform 4)	Ubiquitination	-	75.77	21890473
40	Ubiquitination	WKPPQGTDSIKMENG CCCCCCCCCEEEECC	56.01	29967540
41	Phosphorylation	KPPQGTDSIKMENGQ CCCCCCCCEEEECCC	24.77	25627689
42	Ubiquitination	PPQGTDSIKMENGQS CCCCCCCEEEECCCC	5.69	23000965
43	Sumoylation	PQGTDSIKMENGQST CCCCCCEEEECCCCH	44.08	-
43	Sumoylation	PQGTDSIKMENGQST CCCCCCEEEECCCCH	44.08	28112733
43	Ubiquitination	PQGTDSIKMENGQST CCCCCCEEEECCCCH	44.08	29967540
44 (in isoform 4)	Phosphorylation	-	4.63	27251275
51	Ubiquitination	MENGQSTAAKLGLPP EECCCCHHHHHCCCC	14.08	23000965
51 (in isoform 5)	Ubiquitination	-	14.08	21890473
51 (in isoform 6)	Ubiquitination	-	14.08	21890473
56	Ubiquitination	STAAKLGLPPLTPEQ CHHHHHCCCCCCHHH	5.98	23000965
58 (in isoform 6)	Phosphorylation	-	54.64	27251275
60	Phosphorylation	KLGLPPLTPEQQEAL HHCCCCCCHHHHHHH	30.47	30266825
60 (in isoform 2)	Phosphorylation	-	30.47	-
68	Ubiquitination	PEQQEALQKAKKYAM HHHHHHHHHHHHHHH	51.19	29967540
69	Acetylation	EQQEALQKAKKYAME HHHHHHHHHHHHHHH	64.12	26051181
69	Ubiquitination	EQQEALQKAKKYAME HHHHHHHHHHHHHHH	64.12	29967540
71	Ubiquitination	QEALQKAKKYAMEQS HHHHHHHHHHHHHHH	54.04	29967540
72	2-Hydroxyisobutyrylation	EALQKAKKYAMEQSI HHHHHHHHHHHHHHH	43.00	-
72	Acetylation	EALQKAKKYAMEQSI HHHHHHHHHHHHHHH	43.00	27452117
72	Methylation	EALQKAKKYAMEQSI HHHHHHHHHHHHHHH	43.00	-
72	Ubiquitination	EALQKAKKYAMEQSI HHHHHHHHHHHHHHH	43.00	29967540
73	Phosphorylation	ALQKAKKYAMEQSIK HHHHHHHHHHHHHHH	15.52	28509920
78	Phosphorylation	KKYAMEQSIKSVLVK HHHHHHHHHHHHHHH	20.76	29978859
79	Ubiquitination	KYAMEQSIKSVLVKQ HHHHHHHHHHHHHHH	3.73	23000965
80	Sumoylation	YAMEQSIKSVLVKQT HHHHHHHHHHHHHHH	39.50	28112733
80	Ubiquitination	YAMEQSIKSVLVKQT HHHHHHHHHHHHHHH	39.50	23000965
80 (in isoform 1)	Ubiquitination	-	39.50	21890473
80 (in isoform 2)	Ubiquitination	-	39.50	21890473
81	Phosphorylation	AMEQSIKSVLVKQTI HHHHHHHHHHHHHHH	21.00	20860994
84	Ubiquitination	QSIKSVLVKQTIAHQ HHHHHHHHHHHHHHH	3.96	23000965
85	Ubiquitination	SIKSVLVKQTIAHQQ HHHHHHHHHHHHHHH	37.25	23000965
87	Phosphorylation	KSVLVKQTIAHQQQQ HHHHHHHHHHHHHHH	17.89	24043423
87 (in isoform 2)	Phosphorylation	-	17.89	27251275
96	Phosphorylation	AHQQQQLTNLQMAAV HHHHHHHHHHHHHHH	29.55	24043423
104	Phosphorylation	NLQMAAVTMGFGDPL HHHHHHHHCCCCCCC	13.57	28102081
105	Ubiquitination	LQMAAVTMGFGDPLS HHHHHHHCCCCCCCC	3.17	23000965
108	Ubiquitination	AAVTMGFGDPLSPLQ HHHHCCCCCCCCHHH	29.87	29967540
110	Ubiquitination	VTMGFGDPLSPLQSM HHCCCCCCCCHHHHH	34.81	23000965
112	Phosphorylation	MGFGDPLSPLQSMAA CCCCCCCCHHHHHHH	29.02	20068231
116	Phosphorylation	DPLSPLQSMAAQRQR CCCCHHHHHHHHHHH	20.33	24043423
117	Ubiquitination	PLSPLQSMAAQRQRA CCCHHHHHHHHHHHH	2.00	23000965
122	Ubiquitination	QSMAAQRQRALAIMC HHHHHHHHHHHHHHH	23.95	23000965
125	Ubiquitination	AAQRQRALAIMCRVY HHHHHHHHHHHHHHH	3.46	29967540
139	Ubiquitination	YVGSIYYELGEDTIR HHHHHHHHCCCCHHH	33.07	29967540
150	Ubiquitination	DTIRQAFAPFGPIKS CHHHHHHCCCCCCEE	11.09	29967540
151	Ubiquitination	TIRQAFAPFGPIKSI HHHHHHCCCCCCEEE	28.42	29967540
157	Phosphorylation	APFGPIKSIDMSWDS CCCCCCEEEECCCCC	25.25	21601212
167	Ubiquitination	MSWDSVTMKHKGFAF CCCCCEEECCCCEEE	3.92	29967540
168	Ubiquitination	SWDSVTMKHKGFAFV CCCCEEECCCCEEEE	32.57	29967540
172 (in isoform 5)	Ubiquitination	-	5.94	-
177	Phosphorylation	KGFAFVEYEVPEAAQ CCEEEEEEECHHHHH	19.54	-
183	Ubiquitination	EYEVPEAAQLALEQM EEECHHHHHHHHHHH	11.85	21963094
189 (in isoform 2)	Phosphorylation	-	36.89	-
191	Acetylation	QLALEQMNSVMLGGR HHHHHHHHCCEECCC	31.01	19608861
191	Ubiquitination	QLALEQMNSVMLGGR HHHHHHHHCCEECCC	31.01	33845483
191 (in isoform 4)	Ubiquitination	-	31.01	21890473
197	Ubiquitination	MNSVMLGGRNIKVGR HHCCEECCCCCCCCC	18.59	21963094
200	Ubiquitination	VMLGGRNIKVGRPSN CEECCCCCCCCCCCC	3.54	21963094
201	Acetylation	MLGGRNIKVGRPSNI EECCCCCCCCCCCCC	42.03	25953088
201	Ubiquitination	MLGGRNIKVGRPSNI EECCCCCCCCCCCCC	42.03	-
205	Acetylation	RNIKVGRPSNIGQAQ CCCCCCCCCCCCCCH	26.29	19608861
205	Ubiquitination	RNIKVGRPSNIGQAQ CCCCCCCCCCCCCCH	26.29	33845483
205 (in isoform 6)	Ubiquitination	-	26.29	21890473
206	Phosphorylation	NIKVGRPSNIGQAQP CCCCCCCCCCCCCHH	39.45	25159151
207	Ubiquitination	IKVGRPSNIGQAQPI CCCCCCCCCCCCHHH	45.13	33845483
208	Acetylation	KVGRPSNIGQAQPII CCCCCCCCCCCHHHH	5.19	19608861
208	Ubiquitination	KVGRPSNIGQAQPII CCCCCCCCCCCHHHH	5.19	33845483
208 (in isoform 3)	Ubiquitination	-	5.19	21890473
214	Ubiquitination	NIGQAQPIIDQLAEE CCCCCHHHHHHHHHH	3.45	21963094
214 (in isoform 5)	Ubiquitination	-	3.45	-
219	Ubiquitination	QPIIDQLAEEARAFN HHHHHHHHHHHHHHC	13.62	23503661
221	Ubiquitination	IIDQLAEEARAFNRI HHHHHHHHHHHHCCE	37.54	33845483
222	Acetylation	IDQLAEEARAFNRIY HHHHHHHHHHHCCEE	10.29	19608861
222	Ubiquitination	IDQLAEEARAFNRIY HHHHHHHHHHHCCEE	10.29	33845483
222 (in isoform 5)	Ubiquitination	-	10.29	21890473
224	Ubiquitination	QLAEEARAFNRIYVA HHHHHHHHHCCEEEE	17.08	33845483
224 (in isoform 5)	Ubiquitination	-	17.08	-
225	Ubiquitination	LAEEARAFNRIYVAS HHHHHHHHCCEEEEE	5.42	21963094
226	Ubiquitination	AEEARAFNRIYVASV HHHHHHHCCEEEEEE	28.58	21963094
226 (in isoform 2)	Ubiquitination	-	28.58	-
227 (in isoform 2)	Phosphorylation	-	18.37	21406692
232	Phosphorylation	FNRIYVASVHQDLSD HCCEEEEEECCCCCH	15.30	20873877
233	Acetylation	NRIYVASVHQDLSDD CCEEEEEECCCCCHH	3.20	19608861
233	Ubiquitination	NRIYVASVHQDLSDD CCEEEEEECCCCCHH	3.20	33845483
234	Acetylation	RIYVASVHQDLSDDD CEEEEEECCCCCHHH	16.97	19608861
234	Ubiquitination	RIYVASVHQDLSDDD CEEEEEECCCCCHHH	16.97	33845483
234 (in isoform 2)	Acetylation	-	16.97	-
234 (in isoform 2)	Ubiquitination	-	16.97	21890473
236	Ubiquitination	YVASVHQDLSDDDIK EEEEECCCCCHHHHH	33.01	23503661
238	Phosphorylation	ASVHQDLSDDDIKSV EEECCCCCHHHHHHH	47.48	20873877
238	Ubiquitination	ASVHQDLSDDDIKSV EEECCCCCHHHHHHH	47.48	33845483
238 (in isoform 5)	Ubiquitination	-	47.48	-
241	Ubiquitination	HQDLSDDDIKSVFEA CCCCCHHHHHHHHHH	56.03	21963094
242	Ubiquitination	QDLSDDDIKSVFEAF CCCCHHHHHHHHHHH	4.63	21963094
243	Acetylation	DLSDDDIKSVFEAFG CCCHHHHHHHHHHHH	47.78	26051181
243	Ubiquitination	DLSDDDIKSVFEAFG CCCHHHHHHHHHHHH	47.78	21963094
244	Phosphorylation	LSDDDIKSVFEAFGK CCHHHHHHHHHHHHC	32.16	30266825
247	Ubiquitination	DDIKSVFEAFGKIKS HHHHHHHHHHHCCEE	40.72	23503661
248	Ubiquitination	DIKSVFEAFGKIKSC HHHHHHHHHHCCEEE	13.66	23503661
249	Ubiquitination	IKSVFEAFGKIKSCT HHHHHHHHHCCEEEE	8.90	33845483
250	Acetylation	KSVFEAFGKIKSCTL HHHHHHHHCCEEEEE	37.51	19608861
250	Ubiquitination	KSVFEAFGKIKSCTL HHHHHHHHCCEEEEE	37.51	33845483
250 (in isoform 2)	Ubiquitination	-	37.51	-
251	Acetylation	SVFEAFGKIKSCTLA HHHHHHHCCEEEEEE	40.27	19608861
251	Ubiquitination	SVFEAFGKIKSCTLA HHHHHHHCCEEEEEE	40.27	33845483
251 (in isoform 1)	Ubiquitination	-	40.27	21890473
255	Ubiquitination	AFGKIKSCTLARDPT HHHCCEEEEEEECCC	2.84	21963094
258	Ubiquitination	KIKSCTLARDPTTGK CCEEEEEEECCCCCC	8.83	21963094
262	Phosphorylation	CTLARDPTTGKHKGY EEEEECCCCCCCCCC	53.09	-
263	Phosphorylation	TLARDPTTGKHKGYG EEEECCCCCCCCCCC	50.01	-
263	Ubiquitination	TLARDPTTGKHKGYG EEEECCCCCCCCCCC	50.01	21963094
264	Ubiquitination	LARDPTTGKHKGYGF EEECCCCCCCCCCCE	32.33	23503661
265	Ubiquitination	ARDPTTGKHKGYGFI EECCCCCCCCCCCEE	39.80	23503661
266	Ubiquitination	RDPTTGKHKGYGFIE ECCCCCCCCCCCEEE	30.07	33845483
267	Acetylation	DPTTGKHKGYGFIEY CCCCCCCCCCCEEEE	58.26	26051181
267	Ubiquitination	DPTTGKHKGYGFIEY CCCCCCCCCCCEEEE	58.26	33845483
268	Ubiquitination	PTTGKHKGYGFIEYE CCCCCCCCCCEEEEE	27.76	21963094
269	Phosphorylation	TTGKHKGYGFIEYEK CCCCCCCCCEEEEEE	17.42	24043423
271	Ubiquitination	GKHKGYGFIEYEKAQ CCCCCCCEEEEEECC	2.63	21890473
272	Ubiquitination	KHKGYGFIEYEKAQS CCCCCCEEEEEECCC	4.84	21963094
274	Phosphorylation	KGYGFIEYEKAQSSQ CCCCEEEEEECCCCC	20.22	24043423
276	Ubiquitination	YGFIEYEKAQSSQDA CCEEEEEECCCCCHH	51.31	21890473
279	Phosphorylation	IEYEKAQSSQDAVSS EEEEECCCCCHHHHH	34.52	21712546
280	Phosphorylation	EYEKAQSSQDAVSSM EEEECCCCCHHHHHC	21.72	21712546
280	Ubiquitination	EYEKAQSSQDAVSSM EEEECCCCCHHHHHC	21.72	21963094
283	Ubiquitination	KAQSSQDAVSSMNLF ECCCCCHHHHHCCHH	8.75	21963094
284	Ubiquitination	AQSSQDAVSSMNLFD CCCCCHHHHHCCHHH	6.15	21963094
284 (in isoform 2)	Ubiquitination	-	6.15	-
285	Phosphorylation	QSSQDAVSSMNLFDL CCCCHHHHHCCHHHC	26.12	24043423
285	Ubiquitination	QSSQDAVSSMNLFDL CCCCHHHHHCCHHHC	26.12	23503661
286	Phosphorylation	SSQDAVSSMNLFDLG CCCHHHHHCCHHHCC	12.70	24043423
287	Ubiquitination	SQDAVSSMNLFDLGG CCHHHHHCCHHHCCC	3.83	33845483
288	Ubiquitination	QDAVSSMNLFDLGGQ CHHHHHCCHHHCCCC	39.23	21890473
290	Ubiquitination	AVSSMNLFDLGGQYL HHHHCCHHHCCCCEE	6.56	23503661
292	Ubiquitination	SSMNLFDLGGQYLRV HHCCHHHCCCCEEEE	6.95	23503661
294 (in isoform 2)	Phosphorylation	-	21.91	-
296	Phosphorylation	LFDLGGQYLRVGKAV HHHCCCCEEEEECCC	10.35	24043423
297 (in isoform 2)	Phosphorylation	-	2.48	-
300	Ubiquitination	GGQYLRVGKAVTPPM CCCEEEEECCCCCCC	13.03	21963094
301	Ubiquitination	GQYLRVGKAVTPPMP CCEEEEECCCCCCCC	36.51	21963094
302	Ubiquitination	QYLRVGKAVTPPMPL CEEEEECCCCCCCCC	12.42	23503661
304	Phosphorylation	LRVGKAVTPPMPLLT EEEECCCCCCCCCCC	26.91	20068231
304	Ubiquitination	LRVGKAVTPPMPLLT EEEECCCCCCCCCCC	26.91	33845483
307	Sulfoxidation	GKAVTPPMPLLTPAT ECCCCCCCCCCCCCC	3.72	21406390
311	Phosphorylation	TPPMPLLTPATPGGL CCCCCCCCCCCCCCC	20.68	28674151
314	Phosphorylation	MPLLTPATPGGLPPA CCCCCCCCCCCCCHH	24.54	28355574
321	Ubiquitination	TPGGLPPAAAVAAAA CCCCCCHHHHHHHHH	13.20	21963094
326	Ubiquitination	PPAAAVAAAAATAKI CHHHHHHHHHHHCCC	7.34	21963094
330	Phosphorylation	AVAAAAATAKITAQE HHHHHHHHCCCCHHH	24.61	26552605
338	Ubiquitination	AKITAQEAVAGAAVL CCCCHHHHHHHHHHH	5.60	21963094
350	Phosphorylation	AVLGTLGTPGLVSPA HHHHCCCCCCCCCHH	19.80	26853621
355	Phosphorylation	LGTPGLVSPALTLAQ CCCCCCCCHHHHHHC	14.97	26074081
359	Phosphorylation	GLVSPALTLAQPLGT CCCCHHHHHHCCCCC	23.35	26074081
389 (in isoform 2)	Phosphorylation	-	37.65	-
394	Acetylation	RPPIPVTIPSVGVVN CCCCCCCCCCCCCCC	2.14	19608861
394	Ubiquitination	RPPIPVTIPSVGVVN CCCCCCCCCCCCCCC	2.14	23000965
398	Ubiquitination	PVTIPSVGVVNPILA CCCCCCCCCCCHHHC	23.22	23000965
406	O-linked_Glycosylation	VVNPILASPPTLGLL CCCHHHCCCCCCCCC	28.15	OGP
406	Phosphorylation	VVNPILASPPTLGLL CCCHHHCCCCCCCCC	28.15	20058876
408	Acetylation	NPILASPPTLGLLEP CHHHCCCCCCCCCCC	37.45	19608861
408	Ubiquitination	NPILASPPTLGLLEP CHHHCCCCCCCCCCC	37.45	23000965
411	Acetylation	LASPPTLGLLEPKKE HCCCCCCCCCCCCCH	30.60	19608861
411	Ubiquitination	LASPPTLGLLEPKKE HCCCCCCCCCCCCCH	30.60	23000965
412	Ubiquitination	ASPPTLGLLEPKKEK CCCCCCCCCCCCCHH	5.72	23000965
415	Ubiquitination	PTLGLLEPKKEKEEE CCCCCCCCCCHHHHH	53.65	23000965
419	Sumoylation	LLEPKKEKEEEELFP CCCCCCHHHHHHHCC	78.34	28112733
425	Acetylation	EKEEEELFPESERPE HHHHHHHCCCCCCHH	7.74	19608861
425	Ubiquitination	EKEEEELFPESERPE HHHHHHHCCCCCCHH	7.74	23000965
428	Phosphorylation	EEELFPESERPEMLS HHHHCCCCCCHHHCC	38.86	30108239
429	Ubiquitination	EELFPESERPEMLSE HHHCCCCCCHHHCCC	71.27	23000965
435	Phosphorylation	SERPEMLSEQEHMSI CCCHHHCCCCCCCCC	35.60	30108239
436	Acetylation	ERPEMLSEQEHMSIS CCHHHCCCCCCCCCC	56.90	19608861
436	Ubiquitination	ERPEMLSEQEHMSIS CCHHHCCCCCCCCCC	56.90	23000965
437	Acetylation	RPEMLSEQEHMSISG CHHHCCCCCCCCCCH	42.38	19608861
437	Ubiquitination	RPEMLSEQEHMSISG CHHHCCCCCCCCCCH	42.38	23000965
437 (in isoform 2)	Acetylation	-	42.38	-
440	Ubiquitination	MLSEQEHMSISGSSA HCCCCCCCCCCHHHH	3.61	23000965
441	Phosphorylation	LSEQEHMSISGSSAR CCCCCCCCCCHHHHH	18.60	30108239
441	Ubiquitination	LSEQEHMSISGSSAR CCCCCCCCCCHHHHH	18.60	23000965
443	Phosphorylation	EQEHMSISGSSARHM CCCCCCCCHHHHHHH	25.89	30108239
443	Ubiquitination	EQEHMSISGSSARHM CCCCCCCCHHHHHHH	25.89	24816145
445	Phosphorylation	EHMSISGSSARHMVM CCCCCCHHHHHHHHH	17.65	30108239
446	Phosphorylation	HMSISGSSARHMVMQ CCCCCHHHHHHHHHH	32.61	30108239
446	Ubiquitination	HMSISGSSARHMVMQ CCCCCHHHHHHHHHH	32.61	24816145
453	Acetylation	SARHMVMQKLLRKQE HHHHHHHHHHHHCCH	23.10	19608861
453	Ubiquitination	SARHMVMQKLLRKQE HHHHHHHHHHHHCCH	23.10	23000965
454	Acetylation	ARHMVMQKLLRKQES HHHHHHHHHHHCCHH	31.24	19608861
454	Ubiquitination	ARHMVMQKLLRKQES HHHHHHHHHHHCCHH	31.24	23000965
457	Ubiquitination	MVMQKLLRKQESTVM HHHHHHHHCCHHHHE	49.39	23000965
458	Sumoylation	VMQKLLRKQESTVMV HHHHHHHCCHHHHEE	59.39	-
458	2-Hydroxyisobutyrylation	VMQKLLRKQESTVMV HHHHHHHCCHHHHEE	59.39	-
458	Sumoylation	VMQKLLRKQESTVMV HHHHHHHCCHHHHEE	59.39	28112733
458	Ubiquitination	VMQKLLRKQESTVMV HHHHHHHCCHHHHEE	59.39	23000965
460	Ubiquitination	QKLLRKQESTVMVLR HHHHHCCHHHHEEEE	51.95	24816145
461	Phosphorylation	KLLRKQESTVMVLRN HHHHCCHHHHEEEEC	24.65	30624053
462	Phosphorylation	LLRKQESTVMVLRNM HHHCCHHHHEEEECC	16.33	23403867
471	Ubiquitination	MVLRNMVDPKDIDDD EEEECCCCHHHCCCC	33.49	24816145
472	Ubiquitination	VLRNMVDPKDIDDDL EEECCCCHHHCCCCC	25.47	24816145
473	Acetylation	LRNMVDPKDIDDDLE EECCCCHHHCCCCCC	63.95	26051181
474	Ubiquitination	RNMVDPKDIDDDLEG ECCCCHHHCCCCCCC	55.74	23000965
478	Ubiquitination	DPKDIDDDLEGEVTE CHHHCCCCCCCCCHH	42.76	23000965
479	Ubiquitination	PKDIDDDLEGEVTEE HHHCCCCCCCCCHHH	13.49	23000965
481	Ubiquitination	DIDDDLEGEVTEECG HCCCCCCCCCHHHHH	41.81	24816145
483	Ubiquitination	DDDLEGEVTEECGKF CCCCCCCCHHHHHCC	13.85	23000965
488	Ubiquitination	GEVTEECGKFGAVNR CCCHHHHHCCCCEEE	31.44	24816145
489	Acetylation	EVTEECGKFGAVNRV CCHHHHHCCCCEEEE	52.89	26051181
489	Ubiquitination	EVTEECGKFGAVNRV CCHHHHHCCCCEEEE	52.89	24816145
491	Ubiquitination	TEECGKFGAVNRVII HHHHHCCCCEEEEEE	31.91	23000965
495	Ubiquitination	GKFGAVNRVIIYQEK HCCCCEEEEEEEECC	17.89	23000965
498	Ubiquitination	GAVNRVIIYQEKQGE CCEEEEEEEECCCCC	2.38	24816145
509	Ubiquitination	KQGEEEDAEIIVKIF CCCCHHCHHHHHHHH	17.11	24816145
512	Ubiquitination	EEEDAEIIVKIFVEF CHHCHHHHHHHHHEE	1.62	24816145
514	Ubiquitination	EDAEIIVKIFVEFSI HCHHHHHHHHHEEEE	21.73	24816145
523	Ubiquitination	FVEFSIASETHKAIQ HHEEEECCCHHHHHH	41.16	24816145
524	Ubiquitination	VEFSIASETHKAIQA HEEEECCCHHHHHHH	46.00	24816145
524 (in isoform 2)	Malonylation	-	46.00	32601280
526	Ubiquitination	FSIASETHKAIQALN EEECCCHHHHHHHHC	17.31	24816145
540	Ubiquitination	NGRWFAGRKVVAEVY CCCCCCCCEEEEEEE	25.76	24816145
541	2-Hydroxyisobutyrylation	GRWFAGRKVVAEVYD CCCCCCCEEEEEEEC	39.89	-
541	Ubiquitination	GRWFAGRKVVAEVYD CCCCCCCEEEEEEEC	39.89	24816145
547	Phosphorylation	RKVVAEVYDQERFDN CEEEEEEECHHHCCC	11.92	-
555	Phosphorylation	DQERFDNSDLSA--- CHHHCCCCCCCC---	41.60	26462736
558	Phosphorylation	RFDNSDLSA------ HCCCCCCCC------	36.60	30108239
561	Ubiquitination	NSDLSA--------- CCCCCC---------		24816145
566	Ubiquitination	A-------------- C--------------		24816145
578	Ubiquitination	-------------------------- --------------------------		24816145

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of PUF60_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of PUF60_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of PUF60_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BORC5_HUMAN	LOH12CR1	physical	16189514
PUF60_HUMAN	PUF60	physical	16189514
S30BP_HUMAN	SAP30BP	physical	16189514
RO60_HUMAN	TROVE2	physical	17353931
OLA1_HUMAN	OLA1	physical	17353931
LA_HUMAN	SSB	physical	17353931
RNZ2_HUMAN	ELAC2	physical	17353931
PDIA1_HUMAN	P4HB	physical	17353931
FUBP2_HUMAN	KHSRP	physical	17353931
IF2B1_HUMAN	IGF2BP1	physical	17353931
HNRPC_HUMAN	HNRNPC	physical	17353931
U2AF2_HUMAN	U2AF2	physical	17353931
HNRPQ_HUMAN	SYNCRIP	physical	17353931
DDX3X_HUMAN	DDX3X	physical	17353931
SF3A3_HUMAN	SF3A3	physical	17353931
IF2B3_HUMAN	IGF2BP3	physical	17353931
IF2B2_HUMAN	IGF2BP2	physical	17353931
SYSM_HUMAN	SARS2	physical	17353931
UGPA_HUMAN	UGP2	physical	17353931
HNRPL_HUMAN	HNRNPL	physical	17353931
SRS11_HUMAN	SRSF11	physical	17353931
TITIN_HUMAN	TTN	physical	17353931
MOV10_HUMAN	MOV10	physical	17353931
EWS_HUMAN	EWSR1	physical	17353931
PABP1_HUMAN	PABPC1	physical	17353931
PABP4_HUMAN	PABPC4	physical	17353931
G3BP1_HUMAN	G3BP1	physical	17353931
STOM_HUMAN	STOM	physical	17353931
MRM3_HUMAN	RNMTL1	physical	17353931
SF3B1_HUMAN	SF3B1	physical	17353931
ROA3_HUMAN	HNRNPA3	physical	17353931
CHD3_HUMAN	CHD3	physical	16169070
VIME_HUMAN	VIM	physical	16169070
U2AF2_HUMAN	U2AF2	physical	22939629
SON_HUMAN	SON	physical	22939629
RL6_HUMAN	RPL6	physical	22939629
S30BP_HUMAN	SAP30BP	physical	18255255
ID3_HUMAN	ID3	physical	18255255
RUXF_HUMAN	SNRPF	physical	22365833
U2AF2_HUMAN	U2AF2	physical	22365833
PUF60_HUMAN	PUF60	physical	22365833
S30BP_HUMAN	SAP30BP	physical	22365833
PTBP2_HUMAN	PTBP2	physical	22365833
SIAH1_HUMAN	SIAH1	physical	21988832
NIBL1_HUMAN	FAM129B	physical	22863883
H33_HUMAN	H3F3A	physical	22863883
HIRP3_HUMAN	HIRIP3	physical	22863883
NUDC_HUMAN	NUDC	physical	22863883
PAPOA_HUMAN	PAPOLA	physical	22863883
RO60_HUMAN	TROVE2	physical	22863883
ZYX_HUMAN	ZYX	physical	22863883
PUF60_HUMAN	PUF60	physical	25416956
BLOM7_HUMAN	KIAA0907	physical	25416956
RENT2_HUMAN	UPF2	physical	25416956
S30BP_HUMAN	SAP30BP	physical	25416956
QRIC1_HUMAN	QRICH1	physical	25416956
MED28_HUMAN	MED28	physical	25416956
K1683_HUMAN	KIAA1683	physical	25416956
BORC5_HUMAN	LOH12CR1	physical	25416956
ARL8A_HUMAN	ARL8A	physical	25416956
ZG16_HUMAN	ZG16	physical	25416956
AIMP2_HUMAN	AIMP2	physical	26344197
MY18A_HUMAN	MYO18A	physical	26344197
PR40A_HUMAN	PRPF40A	physical	26344197
SF3A3_HUMAN	SF3A3	physical	26344197
SF3B1_HUMAN	SF3B1	physical	26344197
SRRM2_HUMAN	SRRM2	physical	26344197
PUF60_HUMAN	PUF60	physical	21516116
S30BP_HUMAN	SAP30BP	physical	21516116
GDS1_HUMAN	RAP1GDS1	physical	21516116
SIAH1_HUMAN	SIAH1	physical	21516116
MED28_HUMAN	MED28	physical	21516116

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
615583	Verheij syndrome (VRJS)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of PUF60_HUMAN

Related Literatures of Post-Translational Modification

Acetylation
Reference	PubMed
"Lysine acetylation targets protein complexes and co-regulates majorcellular functions."; Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.; Science 325:834-840(2009). Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-251 AND LYS-454, AND MASSSPECTROMETRY.	19608861 [Abstract]
Phosphorylation
Reference	PubMed
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-60 AND SER-206, AND MASSSPECTROMETRY.	18669648 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-60, AND MASSSPECTROMETRY.	19413330 [Abstract]
"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization."; Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; Nat. Biotechnol. 24:1285-1292(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-60, AND MASSSPECTROMETRY.	16964243 [Abstract]