dbPTM

IF2B3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	IF2B3_HUMAN
UniProt AC	O00425
Protein Name	Insulin-like growth factor 2 mRNA-binding protein 3
Gene Name	IGF2BP3
Organism	Homo sapiens (Human).
Sequence Length	579
Subcellular Localization	Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece an
Protein Description	RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs..
Protein Sequence	MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAIIGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	Phosphorylation	---MNKLYIGNLSEN ---CCCEEECCCCCC	14.37	20873877
10	Phosphorylation	KLYIGNLSENAAPSD CEEECCCCCCCCCCH	32.57	20873877
16	Phosphorylation	LSENAAPSDLESIFK CCCCCCCCHHHHHHH	50.53	20873877
20	Phosphorylation	AAPSDLESIFKDAKI CCCCHHHHHHHCCCC	40.39	24719451
23	Ubiquitination	SDLESIFKDAKIPVS CHHHHHHHCCCCCCC	55.92	21906983
23 (in isoform 1)	Ubiquitination	-	55.92	21906983
26	Ubiquitination	ESIFKDAKIPVSGPF HHHHHCCCCCCCCCE	58.64	27667366
30	Phosphorylation	KDAKIPVSGPFLVKT HCCCCCCCCCEEEEE	35.08	21406692
33	Ubiquitination	KIPVSGPFLVKTGYA CCCCCCCEEEEECEE	16.28	27667366
36	Ubiquitination	VSGPFLVKTGYAFVD CCCCEEEEECEEEEE	38.08	32015554
39	Phosphorylation	PFLVKTGYAFVDCPD CEEEEECEEEEECCC	11.33	23822953
44	Glutathionylation	TGYAFVDCPDESWAL ECEEEEECCCHHHHH	3.73	22555962
45	Ubiquitination	GYAFVDCPDESWALK CEEEEECCCHHHHHH	43.89	21890473
52	Ubiquitination	PDESWALKAIEALSG CCHHHHHHHHHHHCC	39.30	32015554
56	Ubiquitination	WALKAIEALSGKIEL HHHHHHHHHCCCEEE	10.83	21890473
59 (in isoform 2)	Ubiquitination	-	35.49	21906983
66	Acetylation	GKIELHGKPIEVEHS CCEEECCCEEEEECC	31.88	26051181
66	Ubiquitination	GKIELHGKPIEVEHS CCEEECCCEEEEECC	31.88	22053931
69 (in isoform 2)	Ubiquitination	-	50.83	21906983
71	Ubiquitination	HGKPIEVEHSVPKRQ CCCEEEEECCCCHHH	20.04	22817900
73	Phosphorylation	KPIEVEHSVPKRQRI CEEEEECCCCHHHHH	26.98	20873877
76	Ubiquitination	EVEHSVPKRQRIRKL EEECCCCHHHHHHHC	60.03	29967540
82	Ubiquitination	PKRQRIRKLQIRNIP CHHHHHHHCCCCCCC	42.23	27667366
84 (in isoform 2)	Ubiquitination	-	35.31	21906983
97	Ubiquitination	PHLQWEVLDSLLVQY HHHCHHHHHHHHHHH	2.22	27667366
105	Ubiquitination	DSLLVQYGVVESCEQ HHHHHHHCCEECEEE	10.41	21890473
109	Ubiquitination	VQYGVVESCEQVNTD HHHCCEECEEECCCC	16.02	23503661
115	Ubiquitination	ESCEQVNTDSETAVV ECEEECCCCCCEEEE	41.88	27667366
120	Ubiquitination	VNTDSETAVVNVTYS CCCCCCEEEEEEEEC	10.14	21890473
123	Ubiquitination	DSETAVVNVTYSSKD CCCEEEEEEEECCHH	17.51	21890473
129	Acetylation	VNVTYSSKDQARQAL EEEEECCHHHHHHHH	48.17	30589459
130	Ubiquitination	NVTYSSKDQARQALD EEEECCHHHHHHHHH	49.69	22053931
135	Ubiquitination	SKDQARQALDKLNGF CHHHHHHHHHHHCCC	16.51	22817900
138	Ubiquitination	QARQALDKLNGFQLE HHHHHHHHHCCCEEE	44.71	22817900
143	Ubiquitination	LDKLNGFQLENFTLK HHHHCCCEEECEEEE	49.63	21890473
158	Sulfoxidation	VAYIPDEMAAQQNPL EEECCHHHHHHHCCC	4.96	21406390
158	Ubiquitination	VAYIPDEMAAQQNPL EEECCHHHHHHHCCC	4.96	23503661
164	Ubiquitination	EMAAQQNPLQQPRGR HHHHHHCCCCCCCCC	27.49	27667366
172	Ubiquitination	LQQPRGRRGLGQRGS CCCCCCCCCCCCCCC	47.73	21890473
173	Ubiquitination	QQPRGRRGLGQRGSS CCCCCCCCCCCCCCC	32.57	23503661
179	Phosphorylation	RGLGQRGSSRQGSPG CCCCCCCCCCCCCCC	25.24	29396449
179	Ubiquitination	RGLGQRGSSRQGSPG CCCCCCCCCCCCCCC	25.24	27667366
180	Phosphorylation	GLGQRGSSRQGSPGS CCCCCCCCCCCCCCC	31.29	26055452
184	Phosphorylation	RGSSRQGSPGSVSKQ CCCCCCCCCCCCCCC	20.11	21955146
186 (in isoform 2)	Ubiquitination	-	42.23	21906983
187	Phosphorylation	SRQGSPGSVSKQKPC CCCCCCCCCCCCCCC	26.97	28176443
187	Ubiquitination	SRQGSPGSVSKQKPC CCCCCCCCCCCCCCC	26.97	21890473
189	Phosphorylation	QGSPGSVSKQKPCDL CCCCCCCCCCCCCCC	30.94	28176443
190	Acetylation	GSPGSVSKQKPCDLP CCCCCCCCCCCCCCC	61.25	25953088
190	Ubiquitination	GSPGSVSKQKPCDLP CCCCCCCCCCCCCCC	61.25	32015554
192	Ubiquitination	PGSVSKQKPCDLPLR CCCCCCCCCCCCCEE	51.88	32015554
195	Ubiquitination	VSKQKPCDLPLRLLV CCCCCCCCCCEEEEE	60.90	27667366
213	Ubiquitination	FVGAIIGKEGATIRN HHHHHHCCCCCCHHH	42.43	22817900
213 (in isoform 1)	Ubiquitination	-	42.43	21906983
218	Ubiquitination	IGKEGATIRNITKQT HCCCCCCHHHHHHHH	2.92	21890473
223	Neddylation	ATIRNITKQTQSKID CCHHHHHHHHHCCEE	47.77	32015554
223	Ubiquitination	ATIRNITKQTQSKID CCHHHHHHHHHCCEE	47.77	21906983
223 (in isoform 1)	Ubiquitination	-	47.77	21906983
225	Phosphorylation	IRNITKQTQSKIDVH HHHHHHHHHCCEEHH	36.04	20860994
228	Ubiquitination	ITKQTQSKIDVHRKE HHHHHHCCEEHHHHH	32.86	22817900
233	Ubiquitination	QSKIDVHRKENAGAA HCCEEHHHHHCCCCC	48.08	22817900
234	Ubiquitination	SKIDVHRKENAGAAE CCEEHHHHHCCCCCC	40.20	-
242	Ubiquitination	ENAGAAEKSITILST HCCCCCCCEEEEEEC	42.29	29967540
243	Phosphorylation	NAGAAEKSITILSTP CCCCCCCEEEEEECC	19.23	22199227
245	Phosphorylation	GAAEKSITILSTPEG CCCCCEEEEEECCCC	23.98	22199227
248	Phosphorylation	EKSITILSTPEGTSA CCEEEEEECCCCHHH	37.76	20068231
249	Phosphorylation	KSITILSTPEGTSAA CEEEEEECCCCHHHH	23.16	21815630
253	Phosphorylation	ILSTPEGTSAACKSI EEECCCCHHHHHHHH	17.33	20068231
254	Phosphorylation	LSTPEGTSAACKSIL EECCCCHHHHHHHHH	25.40	20068231
257	Glutathionylation	PEGTSAACKSILEIM CCCHHHHHHHHHHHH	3.39	22555962
257	S-nitrosylation	PEGTSAACKSILEIM CCCHHHHHHHHHHHH	3.39	2212679
258	Ubiquitination	EGTSAACKSILEIMH CCHHHHHHHHHHHHH	35.49	29967540
259	Phosphorylation	GTSAACKSILEIMHK CHHHHHHHHHHHHHH	32.08	28555341
266	Ubiquitination	SILEIMHKEAQDIKF HHHHHHHHHHHCCCC	37.05	32015554
271	Ubiquitination	MHKEAQDIKFTEEIP HHHHHHCCCCCCCCC	2.35	23503661
272	Ubiquitination	HKEAQDIKFTEEIPL HHHHHCCCCCCCCCC	55.30	27667366
272 (in isoform 1)	Ubiquitination	-	55.30	21906983
277	Ubiquitination	DIKFTEEIPLKILAH CCCCCCCCCCCHHHC	3.84	27667366
280	Acetylation	FTEEIPLKILAHNNF CCCCCCCCHHHCCCH	30.25	26051181
280	Ubiquitination	FTEEIPLKILAHNNF CCCCCCCCHHHCCCH	30.25	22817900
280 (in isoform 1)	Ubiquitination	-	30.25	21906983
283	Ubiquitination	EIPLKILAHNNFVGR CCCCCHHHCCCHHHH	13.56	21890473
285	Ubiquitination	PLKILAHNNFVGRLI CCCHHHCCCHHHHHH	37.72	21890473
293	Ubiquitination	NFVGRLIGKEGRNLK CHHHHHHCCCCCCCH	26.93	27667366
294	Ubiquitination	FVGRLIGKEGRNLKK HHHHHHCCCCCCCHH	50.42	-
300	Ubiquitination	GKEGRNLKKIEQDTD CCCCCCCHHCCCCCC	56.08	-
301	Ubiquitination	KEGRNLKKIEQDTDT CCCCCCHHCCCCCCC	55.42	29967540
308	Ubiquitination	KIEQDTDTKITISPL HCCCCCCCCEEECCC	26.83	21890473
309	Ubiquitination	IEQDTDTKITISPLQ CCCCCCCCEEECCCC	40.43	29967540
311	Phosphorylation	QDTDTKITISPLQEL CCCCCCEEECCCCEE	19.56	28122231
313	Phosphorylation	TDTKITISPLQELTL CCCCEEECCCCEEEE	15.77	28122231
318	Ubiquitination	TISPLQELTLYNPER EECCCCEEEECCCCC	2.29	27667366
321	Ubiquitination	PLQELTLYNPERTIT CCCEEEECCCCCEEE	24.36	27667366
330	Ubiquitination	PERTITVKGNVETCA CCCEEEEECCHHHHH	35.80	32015554
332	Ubiquitination	RTITVKGNVETCAKA CEEEEECCHHHHHHH	23.80	21890473
336	Ubiquitination	VKGNVETCAKAEEEI EECCHHHHHHHHHHH	2.01	27667366
338	Ubiquitination	GNVETCAKAEEEIMK CCHHHHHHHHHHHHH	59.48	29967540
342	Ubiquitination	TCAKAEEEIMKKIRE HHHHHHHHHHHHHHH	39.63	27667366
347	Ubiquitination	EEEIMKKIRESYEND HHHHHHHHHHHHHHC	5.01	21890473
348	Ubiquitination	EEIMKKIRESYENDI HHHHHHHHHHHHHCH	35.37	24816145
351	Ubiquitination	MKKIRESYENDIASM HHHHHHHHHHCHHHH	17.63	23503661
357	Ubiquitination	SYENDIASMNLQAHL HHHHCHHHHHHHHHC	14.45	27667366
367	Ubiquitination	LQAHLIPGLNLNALG HHHHCCCCCCCCCCC	21.87	27667366
372	Ubiquitination	IPGLNLNALGLFPPT CCCCCCCCCCCCCCC	13.23	21890473
382	Ubiquitination	LFPPTSGMPPPTSGP CCCCCCCCCCCCCCC	4.51	27667366
397	Ubiquitination	PSAMTPPYPQFEQSE CCCCCCCCCCCCCCC	15.74	24816145
400	Ubiquitination	MTPPYPQFEQSETET CCCCCCCCCCCCCCE	8.79	23503661
404	Ubiquitination	YPQFEQSETETVHLF CCCCCCCCCCEEEEE	50.81	23503661
410	Ubiquitination	SETETVHLFIPALSV CCCCEEEEEEEHHHH	3.46	22817900
412	Ubiquitination	TETVHLFIPALSVGA CCEEEEEEEHHHHHH	2.16	24816145
415	Ubiquitination	VHLFIPALSVGAIIG EEEEEEHHHHHHHHC	3.41	23503661
429	Acetylation	GKQGQHIKQLSRFAG CCCCHHHHHHHHHCC	42.96	27452117
429	Ubiquitination	GKQGQHIKQLSRFAG CCCCHHHHHHHHHCC	42.96	27667366
434	Ubiquitination	HIKQLSRFAGASIKI HHHHHHHHCCCCEEE	7.08	27667366
438	Phosphorylation	LSRFAGASIKIAPAE HHHHCCCCEEEECCC	24.40	28355574
440	Ubiquitination	RFAGASIKIAPAEAP HHCCCCEEEECCCCC	30.25	22817900
440 (in isoform 1)	Ubiquitination	-	30.25	21906983
445	Ubiquitination	SIKIAPAEAPDAKVR CEEEECCCCCCCEEE	60.73	21890473
450	2-Hydroxyisobutyrylation	PAEAPDAKVRMVIIT CCCCCCCEEEEEEEE	37.44	-
450	Neddylation	PAEAPDAKVRMVIIT CCCCCCCEEEEEEEE	37.44	32015554
450	Sumoylation	PAEAPDAKVRMVIIT CCCCCCCEEEEEEEE	37.44	28112733
450	Ubiquitination	PAEAPDAKVRMVIIT CCCCCCCEEEEEEEE	37.44	27667366
450 (in isoform 1)	Ubiquitination	-	37.44	21906983
453	Sulfoxidation	APDAKVRMVIITGPP CCCCEEEEEEEECCC	2.58	21406390
453	Ubiquitination	APDAKVRMVIITGPP CCCCEEEEEEEECCC	2.58	23503661
455	Ubiquitination	DAKVRMVIITGPPEA CCEEEEEEEECCCHH	1.38	27667366
459	Ubiquitination	RMVIITGPPEAQFKA EEEEEECCCHHHHHH	18.21	22817900
465	Methylation	GPPEAQFKAQGRIYG CCCHHHHHHCCCEEE	27.93	24711551
465	Ubiquitination	GPPEAQFKAQGRIYG CCCHHHHHHCCCEEE	27.93	22817900
465 (in isoform 1)	Ubiquitination	-	27.93	21906983
468	Ubiquitination	EAQFKAQGRIYGKIK HHHHHHCCCEEEEEC	23.86	23503661
470	Ubiquitination	QFKAQGRIYGKIKEE HHHHCCCEEEEECHH	7.32	21890473
473	Ubiquitination	AQGRIYGKIKEENFV HCCCEEEEECHHCCC	33.36	-
474	Ubiquitination	QGRIYGKIKEENFVS CCCEEEEECHHCCCC	6.19	22817900
475	Sumoylation	GRIYGKIKEENFVSP CCEEEEECHHCCCCC	63.56	-
475	Acetylation	GRIYGKIKEENFVSP CCEEEEECHHCCCCC	63.56	26051181
475	Sumoylation	GRIYGKIKEENFVSP CCEEEEECHHCCCCC	63.56	28112733
475	Ubiquitination	GRIYGKIKEENFVSP CCEEEEECHHCCCCC	63.56	27667366
480	Ubiquitination	KIKEENFVSPKEEVK EECHHCCCCCHHHEE	16.84	27667366
481	Phosphorylation	IKEENFVSPKEEVKL ECHHCCCCCHHHEEE	26.56	21815630
483	Ubiquitination	EENFVSPKEEVKLEA HHCCCCCHHHEEEEE	60.03	33845483
487	Ubiquitination	VSPKEEVKLEAHIRV CCCHHHEEEEEEEEC	43.84	29967540
496	Phosphorylation	EAHIRVPSFAAGRVI EEEEECCCHHHCCEE	25.35	27273156
505	Acetylation	AAGRVIGKGGKTVNE HHCCEECCCCCCHHH	54.06	19828211
505	Ubiquitination	AAGRVIGKGGKTVNE HHCCEECCCCCCHHH	54.06	24816145
508	Acetylation	RVIGKGGKTVNELQN CEECCCCCCHHHHCC	58.95	26051181
508	Ubiquitination	RVIGKGGKTVNELQN CEECCCCCCHHHHCC	58.95	32015554
510	Ubiquitination	IGKGGKTVNELQNLS ECCCCCCHHHHCCCC	6.29	24816145
513	Ubiquitination	GGKTVNELQNLSSAE CCCCHHHHCCCCCCE	3.27	23503661
528	Phosphorylation	VVVPRDQTPDENDQV EEECCCCCCCCCCCE	36.68	29255136
540	Phosphorylation	DQVVVKITGHFYACQ CCEEEEEECCHHHHH	20.91	23663014
544	Phosphorylation	VKITGHFYACQVAQR EEEECCHHHHHHHHH	10.78	23663014
552	Ubiquitination	ACQVAQRKIQEILTQ HHHHHHHHHHHHHHH	36.34	29967540
558	Phosphorylation	RKIQEILTQVKQHQQ HHHHHHHHHHHHHHH	36.66	29514088
561	Ubiquitination	QEILTQVKQHQQQKA HHHHHHHHHHHHHHH	32.04	32015554
566	Ubiquitination	QVKQHQQQKALQSGP HHHHHHHHHHHHCCC	26.81	23503661
567	Ubiquitination	VKQHQQQKALQSGPP HHHHHHHHHHHCCCC	46.88	27667366
567 (in isoform 1)	Ubiquitination	-	46.88	21906983
571	Phosphorylation	QQQKALQSGPPQSRR HHHHHHHCCCCCCCC	54.86	20068231
572	Ubiquitination	QQKALQSGPPQSRRK HHHHHHCCCCCCCCC	25.58	22817900
576	Phosphorylation	LQSGPPQSRRK---- HHCCCCCCCCC----	39.57	25159151

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of IF2B3_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of IF2B3_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of IF2B3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
LAP4A_HUMAN	LAPTM4A	physical	22939629
LGMN_HUMAN	LGMN	physical	22939629
UBP16_HUMAN	USP16	physical	22939629
NDUF4_HUMAN	NDUFAF4	physical	22939629
QOR_HUMAN	CRYZ	physical	22863883
ROA2_HUMAN	HNRNPA2B1	physical	22863883
HNRPM_HUMAN	HNRNPM	physical	22863883
HNRPU_HUMAN	HNRNPU	physical	22863883
SYIC_HUMAN	IARS	physical	22863883
K1C17_HUMAN	KRT17	physical	22863883
MRE11_HUMAN	MRE11A	physical	22863883
PA2G4_HUMAN	PA2G4	physical	22863883
RL23A_HUMAN	RPL23A	physical	22863883
PIHD2_HUMAN	PIH1D2	physical	25416956
G3BP2_HUMAN	G3BP2	physical	26344197
RAB2A_HUMAN	RAB2A	physical	26344197
RL37A_HUMAN	RPL37A	physical	26344197
SH3BG_HUMAN	SH3BGR	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of IF2B3_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-528, AND MASSSPECTROMETRY.	19690332 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-528, AND MASSSPECTROMETRY.	18669648 [Abstract]