ECD_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: ECD_HUMAN
• UniProt AC: O95905
• Protein Name: Protein ecdysoneless homolog {ECO:0000250|UniProtKB:Q9W032}
• Gene Name: ECD
• Organism: Homo sapiens (Human).
• Sequence Length: 644
• Subcellular Localization: Cytoplasm . Nucleus . Predominantly is located in the cytoplasm.
• Protein Description: Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. [PubMed: 16849563]
• Protein Sequence: MEETMKLATMEDTVEYCLFLIPDESRDSDKHKEILQKYIERIITRFAPMLVPYIWQNQPFNLKYKPGKGGVPAHMFGVTKFGDNIEDEWFIVYVIKQITKEFPELVARIEDNDGEFLLIEAADFLPKWLDPENSTNRVFFCHGELCIIPAPRKSGAESWLPTTPPTIPQALNIITAHSEKILASESIRAAVNRRIRGYPEKIQASLHRAHCFLPAGIVAVLKQRPRLVAAAVQAFYLRDPIDLRACRVFKTFLPETRIMTSVTFTKCLYAQLVQQRFVPDRRSGYRLPPPSDPQYRAHELGMKLAHGFEILCSKCSPHFSDCKKSLVTASPLWASFLESLKKNDYFKGLIEGSAQYRERLEMAENYFQLSVDWPESSLAMSPGEEILTLLQTIPFDIEDLKKEAANLPPEDDDQWLDLSPDQLDQLLQEAVGKKESESVSKEEKEQNYDLTEVSESMKAFISKVSTHKGAELPREPSEAPITFDADSFLNYFDKILGPRPNESDSDDLDDEDFECLDSDDDLDFETHEPGEEASLKGTLDNLKSYMAQMDQELAHTCISKSFTTRNQVEPVSQTTDNNSDEEDSGTGESVMAPVDVDLNLVSNILESYSSQAGLAGPASNLLQSMGVQLPDNTDHRPTSKPTKN

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
9	Phosphorylation	EETMKLATMEDTVEY CCCCCHHCHHHHHHH		26503514
13	Phosphorylation	KLATMEDTVEYCLFL CHHCHHHHHHHHEEE		26503514
16	Phosphorylation	TMEDTVEYCLFLIPD CHHHHHHHHEEECCC		20068231
25	Phosphorylation	LFLIPDESRDSDKHK EEECCCCCCCCHHHH		20068231
32	Ubiquitination	SRDSDKHKEILQKYI CCCCHHHHHHHHHHH		29967540
63	Ubiquitination	QNQPFNLKYKPGKGG CCCCCCCCCCCCCCC		22817900
65	Ubiquitination	QPFNLKYKPGKGGVP CCCCCCCCCCCCCCC		21963094
68	Ubiquitination	NLKYKPGKGGVPAHM CCCCCCCCCCCCHHH		22817900
100	Ubiquitination	YVIKQITKEFPELVA HHHHHHHHHCHHHHH		27667366
153	Ubiquitination	CIIPAPRKSGAESWL EEEECCCCCCCCCCC		29967540
154	Phosphorylation	IIPAPRKSGAESWLP EEECCCCCCCCCCCC		22199227
158	Phosphorylation	PRKSGAESWLPTTPP CCCCCCCCCCCCCCC		22199227
162	Phosphorylation	GAESWLPTTPPTIPQ CCCCCCCCCCCCHHH		22199227
163	Phosphorylation	AESWLPTTPPTIPQA CCCCCCCCCCCHHHH		22199227
166	Phosphorylation	WLPTTPPTIPQALNI CCCCCCCCHHHHHHH		20068231
175	Phosphorylation	PQALNIITAHSEKIL HHHHHHHHHCCHHHH		20068231
178	Phosphorylation	LNIITAHSEKILASE HHHHHHCCHHHHCCH		-
184	Phosphorylation	HSEKILASESIRAAV CCHHHHCCHHHHHHH		20068231
186	Phosphorylation	EKILASESIRAAVNR HHHHCCHHHHHHHHH		20068231
201	Ubiquitination	RIRGYPEKIQASLHR HHCCCCHHHHHHHHH		27667366
205	Phosphorylation	YPEKIQASLHRAHCF CCHHHHHHHHHHHHC		20860994
222	Acetylation	AGIVAVLKQRPRLVA HHHHHHHHCCHHHHH		12436261
250	Ubiquitination	LRACRVFKTFLPETR HHHHHHHHHHCCCCC		-
280	Ubiquitination	VQQRFVPDRRSGYRL HHHCCCCCCCCCCCC		29967540
283	Phosphorylation	RFVPDRRSGYRLPPP CCCCCCCCCCCCCCC		24043423
285	Phosphorylation	VPDRRSGYRLPPPSD CCCCCCCCCCCCCCC		24043423
291	Phosphorylation	GYRLPPPSDPQYRAH CCCCCCCCCHHHHHH		22210691
295	Phosphorylation	PPPSDPQYRAHELGM CCCCCHHHHHHHHHH		24043423
299	Ubiquitination	DPQYRAHELGMKLAH CHHHHHHHHHHHHHH		27667366
303	Ubiquitination	RAHELGMKLAHGFEI HHHHHHHHHHHHHHH		-
304	Ubiquitination	AHELGMKLAHGFEIL HHHHHHHHHHHHHHH		21890473
314	Ubiquitination	GFEILCSKCSPHFSD HHHHHHHCCCCCHHH		29967540
323	Ubiquitination	SPHFSDCKKSLVTAS CCCHHHHHHHHHHCC		29967540
325	Phosphorylation	HFSDCKKSLVTASPL CHHHHHHHHHHCCHH		20068231
328	Phosphorylation	DCKKSLVTASPLWAS HHHHHHHHCCHHHHH		20068231
330	Phosphorylation	KKSLVTASPLWASFL HHHHHHCCHHHHHHH		20068231
335	Phosphorylation	TASPLWASFLESLKK HCCHHHHHHHHHHHC		20068231
339	Phosphorylation	LWASFLESLKKNDYF HHHHHHHHHHCCCCH		20068231
342	Ubiquitination	SFLESLKKNDYFKGL HHHHHHHCCCCHHHH		27667366
347	Ubiquitination	LKKNDYFKGLIEGSA HHCCCCHHHHHCCCH		21890473
347	Ubiquitination	LKKNDYFKGLIEGSA HHCCCCHHHHHCCCH		21890473
359	Ubiquitination	GSAQYRERLEMAENY CCHHHHHHHHHHHHH		29967540
390	Ubiquitination	GEEILTLLQTIPFDI HHHHHHHHHHCCCCH		29967540
391	Ubiquitination	EEILTLLQTIPFDIE HHHHHHHHHCCCCHH		29967540
398	Ubiquitination	QTIPFDIEDLKKEAA HHCCCCHHHHHHHHH		33845483
401	Ubiquitination	PFDIEDLKKEAANLP CCCHHHHHHHHHCCC		33845483
402	Ubiquitination	FDIEDLKKEAANLPP CCHHHHHHHHHCCCC		29967540
405 (in isoform 3)	Phosphorylation	-		-
415	Ubiquitination	PPEDDDQWLDLSPDQ CCCCCCCHHCCCHHH		32015554
419	Phosphorylation	DDQWLDLSPDQLDQL CCCHHCCCHHHHHHH		25159151
420	Ubiquitination	DQWLDLSPDQLDQLL CCHHCCCHHHHHHHH		22817900
425	Ubiquitination	LSPDQLDQLLQEAVG CCHHHHHHHHHHHHC		21963094
433	Ubiquitination	LLQEAVGKKESESVS HHHHHHCCHHHCCCC		29967540
434	Ubiquitination	LQEAVGKKESESVSK HHHHHCCHHHCCCCH		29967540
435	Ubiquitination	QEAVGKKESESVSKE HHHHCCHHHCCCCHH		29967540
441	Ubiquitination	KESESVSKEEKEQNY HHHCCCCHHHHHHCC		33845483
444	Ubiquitination	ESVSKEEKEQNYDLT CCCCHHHHHHCCCHH		33845483
448	Phosphorylation	KEEKEQNYDLTEVSE HHHHHHCCCHHHHHH		21945579
451	Phosphorylation	KEQNYDLTEVSESMK HHHCCCHHHHHHHHH		21945579
454	Phosphorylation	NYDLTEVSESMKAFI CCCHHHHHHHHHHHH		21945579
456	Phosphorylation	DLTEVSESMKAFISK CHHHHHHHHHHHHHH		21945579
458	Ubiquitination	TEVSESMKAFISKVS HHHHHHHHHHHHHHH		21906983
463	Ubiquitination	SMKAFISKVSTHKGA HHHHHHHHHHHCCCC		21906983
465	Phosphorylation	KAFISKVSTHKGAEL HHHHHHHHHCCCCCC		30576142
466	Ubiquitination	AFISKVSTHKGAELP HHHHHHHHCCCCCCC		29967540
466	Phosphorylation	AFISKVSTHKGAELP HHHHHHHHCCCCCCC		30576142
467	Ubiquitination	FISKVSTHKGAELPR HHHHHHHCCCCCCCC		29967540
468	Ubiquitination	ISKVSTHKGAELPRE HHHHHHCCCCCCCCC		21963094
474	Ubiquitination	HKGAELPREPSEAPI CCCCCCCCCCCCCCC		33845483
477	Phosphorylation	AELPREPSEAPITFD CCCCCCCCCCCCCCC		28857561
477	Ubiquitination	AELPREPSEAPITFD CCCCCCCCCCCCCCC		33845483
491	Ubiquitination	DADSFLNYFDKILGP CHHHHHHHHHHHHCC		32015554
496	Ubiquitination	LNYFDKILGPRPNES HHHHHHHHCCCCCCC		21890473
496	Ubiquitination	LNYFDKILGPRPNES HHHHHHHHCCCCCCC		22817900
500	Ubiquitination	DKILGPRPNESDSDD HHHHCCCCCCCCCCC		21963094
501	Ubiquitination	KILGPRPNESDSDDL HHHCCCCCCCCCCCC		21963094
503	Phosphorylation	LGPRPNESDSDDLDD HCCCCCCCCCCCCCH		17081983
505	Phosphorylation	PRPNESDSDDLDDED CCCCCCCCCCCCHHH		17081983
517	Ubiquitination	DEDFECLDSDDDLDF HHHCCCCCCCCCCCC		32015554
518	Phosphorylation	EDFECLDSDDDLDFE HHCCCCCCCCCCCCC		19919181
526	Phosphorylation	DDDLDFETHEPGEEA CCCCCCCCCCCCCCH		21406692
534	Phosphorylation	HEPGEEASLKGTLDN CCCCCCHHHHHHHHH		18669648
543	Ubiquitination	KGTLDNLKSYMAQMD HHHHHHHHHHHHHHH		21963094
545	Phosphorylation	TLDNLKSYMAQMDQE HHHHHHHHHHHHHHH		28555341
560	Ubiquitination	LAHTCISKSFTTRNQ HHHHHHHCCCCCCCC		32015554
561	Phosphorylation	AHTCISKSFTTRNQV HHHHHHCCCCCCCCE		21712546
576	Ubiquitination	EPVSQTTDNNSDEED ECCEECCCCCCCCCC		21963094
579	Phosphorylation	SQTTDNNSDEEDSGT EECCCCCCCCCCCCC		22468782
593	Ubiquitination	TGESVMAPVDVDLNL CCCCCEECCCCCHHH		32015554

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
503	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
505	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS
518	S	Phosphorylation	Kinase	CSNK2A1	P68400	GPS

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of ECD_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of ECD_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
TXNIP_HUMAN	TXNIP	physical	23880345
PIHD1_HUMAN	PIH1D1	physical	26711270
PRP8_HUMAN	PRPF8	physical	26711270
RUVB1_HUMAN	RUVBL1	physical	26711270
MYH7_HUMAN	MYH7	physical	28514442
WDR83_HUMAN	WDR83	physical	28514442
U520_HUMAN	SNRNP200	physical	28514442
ZNHI2_HUMAN	ZNHIT2	physical	28514442
PRP8_HUMAN	PRPF8	physical	28514442
NCDN_HUMAN	NCDN	physical	28514442
AAR2_HUMAN	AAR2	physical	28561026
ECD_HUMAN	ECD	physical	28561026
U5S1_HUMAN	EFTUD2	physical	28561026
NONO_HUMAN	NONO	physical	28561026
PARP1_HUMAN	PARP1	physical	28561026
RPA1_HUMAN	POLR1A	physical	28561026
PRP19_HUMAN	PRPF19	physical	28561026
PRP8_HUMAN	PRPF8	physical	28561026
SART3_HUMAN	SART3	physical	28561026
U520_HUMAN	SNRNP200	physical	28561026
SNR40_HUMAN	SNRNP40	physical	28561026
TSSC4_HUMAN	TSSC4	physical	28561026

Drug and Disease Associations

• Kegg Drug
• DrugBank
• There are no disease associations of PTM sites.