U520_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: U520_HUMAN
• UniProt AC: O75643
• Protein Name: U5 small nuclear ribonucleoprotein 200 kDa helicase
• Gene Name: SNRNP200
• Organism: Homo sapiens (Human).
• Sequence Length: 2136
• Subcellular Localization: Nucleus .
• Protein Description: RNA helicase that plays an essential role in pre-mRNA splicing as component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome..
• Protein Sequence: MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
5	O-linked_Glycosylation	---MADVTARSLQYE ---CCCCCHHHHHEE	19.53	23301498
8	Phosphorylation	MADVTARSLQYEYKA CCCCCHHHHHEEECC	19.84	20068231
11	Phosphorylation	VTARSLQYEYKANSN CCHHHHHEEECCCCC	26.90	28796482
13	Phosphorylation	ARSLQYEYKANSNLV HHHHHEEECCCCCEE	15.51	28796482
14	Methylation	RSLQYEYKANSNLVL HHHHEEECCCCCEEE	29.20	115979297
14	Ubiquitination	RSLQYEYKANSNLVL HHHHEEECCCCCEEE	29.20	32015554
17	Phosphorylation	QYEYKANSNLVLQAD HEEECCCCCEEEEEC	36.08	30266825
25	Methylation	NLVLQADRSLIDRTR CEEEEECHHHHHCCC	36.06	115919225
26	Phosphorylation	LVLQADRSLIDRTRR EEEEECHHHHHCCCC	30.17	25159151
37	Phosphorylation	RTRRDEPTGEVLSLV CCCCCCCCCHHHHHH	43.88	27732954
42	Phosphorylation	EPTGEVLSLVGKLEG CCCCHHHHHHHHHHC	26.61	20873877
46	2-Hydroxyisobutyrylation	EVLSLVGKLEGTRMG HHHHHHHHHHCCCCC	35.57	-
46	Acetylation	EVLSLVGKLEGTRMG HHHHHHHHHHCCCCC	35.57	23954790
46	Sumoylation	EVLSLVGKLEGTRMG HHHHHHHHHHCCCCC	35.57	28112733
46	Ubiquitination	EVLSLVGKLEGTRMG HHHHHHHHHHCCCCC	35.57	32015554
50	Phosphorylation	LVGKLEGTRMGDKAQ HHHHHHCCCCCCHHH	14.22	23312004
59	Phosphorylation	MGDKAQRTKPQMQEE CCCHHHHCCHHHHHH	34.69	23312004
83	2-Hydroxyisobutyrylation	EDRHDINKMKGYTLL HHHHHHHHHHHHHHH	42.18	-
102	Phosphorylation	DEMVGIIYKPKTKET HHHCEEEECCCCCCC	21.22	-
133	Glutathionylation	DQPRDILCGAADEVL CCCCHHHHCCHHHHH	3.39	22555962
155	Acetylation	LRDKERRKEIDLLLG HCCHHHHHHHCCCCC	66.30	26051181
169	Phosphorylation	GQTDDTRYHVLVNLG CCCCCCCEEEEEECC	9.81	24532841
177	2-Hydroxyisobutyrylation	HVLVNLGKKITDYGG EEEEECCCCEECCCC	44.14	-
177	Acetylation	HVLVNLGKKITDYGG EEEEECCCCEECCCC	44.14	25953088
177	Ubiquitination	HVLVNLGKKITDYGG EEEEECCCCEECCCC	44.14	33845483
182	Phosphorylation	LGKKITDYGGDKEIQ CCCCEECCCCCHHCC	17.85	22817900
198	Phosphorylation	MDDNIDETYGVNVQF CCCCCCCCCCEEEEE	23.10	26657352
199	Phosphorylation	DDNIDETYGVNVQFE CCCCCCCCCEEEEEE	19.61	27732954
207	Phosphorylation	GVNVQFESDEEEGDE CEEEEEECCCCCCCC	52.16	26657352
217	Phosphorylation	EEGDEDVYGEVREEA CCCCCCCHHHHHCCC	21.64	25137130
225	Phosphorylation	GEVREEASDDDMEGD HHHHCCCCCCCCCCC	44.87	19664994
239	Phosphorylation	DEAVVRCTLSANLVA CCEEEEEEECCCEEE	17.12	23090842
241	Phosphorylation	AVVRCTLSANLVASG EEEEEEECCCEEECC	9.28	20068231
247	Phosphorylation	LSANLVASGELMSSK ECCCEEECCCCCCCC	25.89	20068231
252	Phosphorylation	VASGELMSSKKKDLH EECCCCCCCCCCCCC	52.18	23090842
253	Phosphorylation	ASGELMSSKKKDLHP ECCCCCCCCCCCCCH	34.23	27251275
276	Phosphorylation	QRQLSRFYDDAIVSQ HHHHHHHHHHHHHCH	16.29	29052541
282	Phosphorylation	FYDDAIVSQKKADEV HHHHHHHCHHHHHHH	30.12	29052541
284	2-Hydroxyisobutyrylation	DDAIVSQKKADEVLE HHHHHCHHHHHHHHH	42.28	-
284	Acetylation	DDAIVSQKKADEVLE HHHHHCHHHHHHHHH	42.28	25953088
284	Ubiquitination	DDAIVSQKKADEVLE HHHHHCHHHHHHHHH	42.28	21906983
284 (in isoform 1)	Ubiquitination	-	42.28	21890473
284 (in isoform 2)	Ubiquitination	-	42.28	21890473
285	2-Hydroxyisobutyrylation	DAIVSQKKADEVLEI HHHHCHHHHHHHHHH	54.13	-
285	Acetylation	DAIVSQKKADEVLEI HHHHCHHHHHHHHHH	54.13	26051181
285	Ubiquitination	DAIVSQKKADEVLEI HHHHCHHHHHHHHHH	54.13	22817900
294	Ubiquitination	DEVLEILKTASDDRE HHHHHHHHHCCCCHH	47.94	-
295	O-linked_Glycosylation	EVLEILKTASDDREC HHHHHHHHCCCCHHH	28.13	23301498
339	Phosphorylation	TLLASAQSEAEKERI HHHHHHCCHHHHHHH	38.19	26546556
349	Acetylation	EKERIMGKMEADPEL HHHHHHHHHHCCHHH	19.04	23236377
349	Ubiquitination	EKERIMGKMEADPEL HHHHHHHHHHCCHHH	19.04	33845483
350	Sulfoxidation	KERIMGKMEADPELS HHHHHHHHHCCHHHH	4.20	28183972
358	Ubiquitination	EADPELSKFLYQLHE HCCHHHHHHHHHHHH	52.60	22817900
358 (in isoform 1)	Ubiquitination	-	52.60	21890473
358 (in isoform 2)	Ubiquitination	-	52.60	21890473
361	Phosphorylation	PELSKFLYQLHETEK HHHHHHHHHHHHHHH	16.50	20068231
368	2-Hydroxyisobutyrylation	YQLHETEKEDLIREE HHHHHHHHHHHHHHH	65.04	-
368	Acetylation	YQLHETEKEDLIREE HHHHHHHHHHHHHHH	65.04	26822725
368	Ubiquitination	YQLHETEKEDLIREE HHHHHHHHHHHHHHH	65.04	33845483
385	Phosphorylation	RRERVRQSRMDTDLE HHHHHHHHHCCCCCC	21.00	29214152
389	Phosphorylation	VRQSRMDTDLETMDL HHHHHCCCCCCCCCC	32.89	26074081
393	Phosphorylation	RMDTDLETMDLDQGG HCCCCCCCCCCCCCC	24.23	26074081
417	Phosphorylation	DLEDLVFTQGSHFMA CHHHHEECCCCHHHC	25.25	-
426	Acetylation	GSHFMANKRCQLPDG CCHHHCCCCCCCCCC	45.04	23954790
426	Ubiquitination	GSHFMANKRCQLPDG CCHHHCCCCCCCCCC	45.04	22817900
426 (in isoform 1)	Ubiquitination	-	45.04	21890473
426 (in isoform 2)	Ubiquitination	-	45.04	21890473
427	Methylation	SHFMANKRCQLPDGS CHHHCCCCCCCCCCC	17.14	115919229
434	Phosphorylation	RCQLPDGSFRRQRKG CCCCCCCCHHHCCCC	23.98	27080861
440	Ubiquitination	GSFRRQRKGYEEVHV CCHHHCCCCCCCCCC	58.46	33845483
451	Acetylation	EVHVPALKPKPFGSE CCCCCCCCCCCCCCH	53.77	25953088
451	Ubiquitination	EVHVPALKPKPFGSE CCCCCCCCCCCCCCH	53.77	33845483
453	Ubiquitination	HVPALKPKPFGSEEQ CCCCCCCCCCCCHHH	51.93	29967540
457	Phosphorylation	LKPKPFGSEEQLLPV CCCCCCCCHHHCCCH	38.04	29255136
466	Ubiquitination	EQLLPVEKLPKYAQA HHCCCHHHCHHHHHH	71.03	23000965
469	Acetylation	LPVEKLPKYAQAGFE CCHHHCHHHHHHCCC	64.75	19608861
469	Ubiquitination	LPVEKLPKYAQAGFE CCHHHCHHHHHHCCC	64.75	23000965
469 (in isoform 1)	Ubiquitination	-	64.75	21890473
469 (in isoform 2)	Ubiquitination	-	64.75	21890473
479	Acetylation	QAGFEGFKTLNRIQS HHCCCHHHHHHHHHH	64.06	25953088
479	Methylation	QAGFEGFKTLNRIQS HHCCCHHHHHHHHHH	64.06	12433335
479	Ubiquitination	QAGFEGFKTLNRIQS HHCCCHHHHHHHHHH	64.06	23000965
479 (in isoform 1)	Ubiquitination	-	64.06	21890473
479 (in isoform 2)	Ubiquitination	-	64.06	21890473
487	Acetylation	TLNRIQSKLYRAALE HHHHHHHHHHHHHHH	32.91	25953088
487	Ubiquitination	TLNRIQSKLYRAALE HHHHHHHHHHHHHHH	32.91	29967540
502	Glutathionylation	TDENLLLCAPTGAGK CCCCEEEECCCCCCH	4.26	22555962
510	Phosphorylation	APTGAGKTNVALMCM CCCCCCHHHHHHHHH	33.73	-
516	Glutathionylation	KTNVALMCMLREIGK HHHHHHHHHHHHHHH	2.12	22555962
523	Acetylation	CMLREIGKHINMDGT HHHHHHHHHCCCCCC	47.15	26051181
527	Sulfoxidation	EIGKHINMDGTINVD HHHHHCCCCCCCCCC	5.07	28183972
540	Phosphorylation	VDDFKIIYIAPMRSL CCCCEEEEEECCHHH	8.43	20068231
546	Phosphorylation	IYIAPMRSLVQEMVG EEEECCHHHHHHHHH	27.00	21712546
551	Sulfoxidation	MRSLVQEMVGSFGKR CHHHHHHHHHHHHHH	2.01	21406390
554	Phosphorylation	LVQEMVGSFGKRLAT HHHHHHHHHHHHHHH	21.29	21712546
557	Acetylation	EMVGSFGKRLATYGI HHHHHHHHHHHHHCE	42.08	25953088
557	Ubiquitination	EMVGSFGKRLATYGI HHHHHHHHHHHHHCE	42.08	23000965
557 (in isoform 1)	Ubiquitination	-	42.08	21890473
557 (in isoform 2)	Ubiquitination	-	42.08	21890473
569 (in isoform 2)	Ubiquitination	-	4.71	21890473
604	Phosphorylation	TRKGGERTYTQLVRL EECCCCCHHHHHHHH	26.98	28152594
605	Phosphorylation	RKGGERTYTQLVRLI ECCCCCHHHHHHHHH	9.94	28152594
606	Phosphorylation	KGGERTYTQLVRLII CCCCCHHHHHHHHHH	18.14	-
641	Sulfoxidation	RAIRNIEMTQEDVRL HHHHCCCCCHHHHHH	4.08	21406390
652	Phosphorylation	DVRLIGLSATLPNYE HHHHHEEECCCCCHH	17.62	28270605
654	Phosphorylation	RLIGLSATLPNYEDV HHHEEECCCCCHHHH	39.10	28270605
658	Phosphorylation	LSATLPNYEDVATFL EECCCCCHHHHHHHE	16.15	28270605
663	Phosphorylation	PNYEDVATFLRVDPA CCHHHHHHHEEEECC	24.05	28270605
695	2-Hydroxyisobutyrylation	TYVGITEKKAIKRFQ EEEEEECHHHHHHHH	38.56	-
709	Phosphorylation	QIMNEIVYEKIMEHA HHHHHHHHHHHHHHC	19.71	-
711	2-Hydroxyisobutyrylation	MNEIVYEKIMEHAGK HHHHHHHHHHHHCCC	29.66	-
711	Acetylation	MNEIVYEKIMEHAGK HHHHHHHHHHHHCCC	29.66	27452117
711	Ubiquitination	MNEIVYEKIMEHAGK HHHHHHHHHHHHCCC	29.66	21906983
711 (in isoform 1)	Ubiquitination	-	29.66	21890473
729	Acetylation	LVFVHSRKETGKTAR EEEEECCCCCCHHHH	63.74	134129
731	Phosphorylation	FVHSRKETGKTARAI EEECCCCCCHHHHHH	47.19	25954137
742	Glutathionylation	ARAIRDMCLEKDTLG HHHHHHHHCCCCCHH	5.34	22555962
745	2-Hydroxyisobutyrylation	IRDMCLEKDTLGLFL HHHHHCCCCCHHHHH	42.96	-
745	Acetylation	IRDMCLEKDTLGLFL HHHHHCCCCCHHHHH	42.96	26051181
745	Ubiquitination	IRDMCLEKDTLGLFL HHHHHCCCCCHHHHH	42.96	29967540
747	Phosphorylation	DMCLEKDTLGLFLRE HHHCCCCCHHHHHCC	33.52	21712546
756	Phosphorylation	GLFLREGSASTEVLR HHHHCCCCCCHHHHH	17.81	29255136
758	Phosphorylation	FLREGSASTEVLRTE HHCCCCCCHHHHHHH	27.14	29255136
759	Phosphorylation	LREGSASTEVLRTEA HCCCCCCHHHHHHHH	29.81	29255136
764	Phosphorylation	ASTEVLRTEAEQCKN CCHHHHHHHHHHHCC	35.07	23312004
770	2-Hydroxyisobutyrylation	RTEAEQCKNLELKDL HHHHHHHCCCCHHHH	65.35	-
770	Acetylation	RTEAEQCKNLELKDL HHHHHHHCCCCHHHH	65.35	25953088
770	Ubiquitination	RTEAEQCKNLELKDL HHHHHHHCCCCHHHH	65.35	23000965
770 (in isoform 1)	Ubiquitination	-	65.35	21890473
775	Ubiquitination	QCKNLELKDLLPYGF HHCCCCHHHHHCCCH	36.63	23000965
795	Phosphorylation	GMTRVDRTLVEDLFA CCCCCCCHHHHHHHC	30.60	21712546
804	Methylation	VEDLFADKHIQVLVS HHHHHCCCCHHHEEH	38.90	-
829	Methylation	PAHTVIIKGTQVYSP CCCEEEEECCEEECC	44.79	24129315
834	Phosphorylation	IIKGTQVYSPEKGRW EEECCEEECCCCCCC	14.53	23312004
835	Phosphorylation	IKGTQVYSPEKGRWT EECCEEECCCCCCCE	27.94	28985074
838	2-Hydroxyisobutyrylation	TQVYSPEKGRWTELG CEEECCCCCCCEEHH	58.10	-
838	Acetylation	TQVYSPEKGRWTELG CEEECCCCCCCEEHH	58.10	27452117
838	Ubiquitination	TQVYSPEKGRWTELG CEEECCCCCCCEEHH	58.10	33845483
922	Phosphorylation	DAVNWLGYAYLYIRM HHHHHHHHHHHHHHH	7.01	17053785
924	Phosphorylation	VNWLGYAYLYIRMLR HHHHHHHHHHHHHHC	7.49	17053785
926	Phosphorylation	WLGYAYLYIRMLRSP HHHHHHHHHHHHCCC	3.62	17053785
932	Phosphorylation	LYIRMLRSPTLYGIS HHHHHHCCCCEECCC	20.48	28152594
934	Phosphorylation	IRMLRSPTLYGISHD HHHHCCCCEECCCHH	33.58	27811184
936	Phosphorylation	MLRSPTLYGISHDDL HHCCCCEECCCHHHC	18.19	27811184
939	Phosphorylation	SPTLYGISHDDLKGD CCCEECCCHHHCCCC	19.25	28152594
944	Sumoylation	GISHDDLKGDPLLDQ CCCHHHCCCCCCCCH	69.16	-
944	Acetylation	GISHDDLKGDPLLDQ CCCHHHCCCCCCCCH	69.16	23954790
944	Malonylation	GISHDDLKGDPLLDQ CCCHHHCCCCCCCCH	69.16	26320211
944	Ubiquitination	GISHDDLKGDPLLDQ CCCHHHCCCCCCCCH	69.16	21963094
944 (in isoform 1)	Ubiquitination	-	69.16	21890473
966	Acetylation	TAALMLDKNNLVKYD HHHHHHCCCCCEEEE	43.72	25953088
966	Ubiquitination	TAALMLDKNNLVKYD HHHHHHCCCCCEEEE	43.72	29967540
971	Sumoylation	LDKNNLVKYDKKTGN HCCCCCEEEECCCCC	51.39	-
971	Acetylation	LDKNNLVKYDKKTGN HCCCCCEEEECCCCC	51.39	19608861
971	Ubiquitination	LDKNNLVKYDKKTGN HCCCCCEEEECCCCC	51.39	19608861
974	Ubiquitination	NNLVKYDKKTGNFQV CCCEEEECCCCCEEE	49.50	-
975	2-Hydroxyisobutyrylation	NLVKYDKKTGNFQVT CCEEEECCCCCEEEE	59.78	-
975	Ubiquitination	NLVKYDKKTGNFQVT CCEEEECCCCCEEEE	59.78	33845483
989	Phosphorylation	TELGRIASHYYITND EEHHEEEEEEEECCH	15.06	27732954
991	Phosphorylation	LGRIASHYYITNDTV HHEEEEEEEECCHHH	8.28	27732954
992	Phosphorylation	GRIASHYYITNDTVQ HEEEEEEEECCHHHH	8.88	27732954
994	Phosphorylation	IASHYYITNDTVQTY EEEEEEECCHHHHHH	15.77	27732954
997	Phosphorylation	HYYITNDTVQTYNQL EEEECCHHHHHHHHH	19.31	27732954
1000	Phosphorylation	ITNDTVQTYNQLLKP ECCHHHHHHHHHHCC	21.80	27732954
1001	Phosphorylation	TNDTVQTYNQLLKPT CCHHHHHHHHHHCCC	5.48	27732954
1021	Phosphorylation	LFRVFSLSSEFKNIT HHHHHCCCHHHCCCC	26.99	21712546
1022	Phosphorylation	FRVFSLSSEFKNITV HHHHCCCHHHCCCCC	52.96	21712546
1025	Ubiquitination	FSLSSEFKNITVREE HCCCHHHCCCCCCHH	43.31	33845483
1034	Acetylation	ITVREEEKLELQKLL CCCCHHHHHHHHHHH	51.01	26051181
1034	Ubiquitination	ITVREEEKLELQKLL CCCCHHHHHHHHHHH	51.01	23000965
1039	Acetylation	EEKLELQKLLERVPI HHHHHHHHHHHCCCC	68.60	26051181
1039	Ubiquitination	EEKLELQKLLERVPI HHHHHHHHHHHCCCC	68.60	23000965
1039 (in isoform 1)	Ubiquitination	-	68.60	21890473
1049	2-Hydroxyisobutyrylation	ERVPIPVKESIEEPS HCCCCCCCCCCCCCC	40.15	-
1049	Acetylation	ERVPIPVKESIEEPS HCCCCCCCCCCCCCC	40.15	26051181
1049	Ubiquitination	ERVPIPVKESIEEPS HCCCCCCCCCCCCCC	40.15	21906983
1049 (in isoform 1)	Ubiquitination	-	40.15	21890473
1056	Phosphorylation	KESIEEPSAKINVLL CCCCCCCCHHHHHHH	45.53	22817900
1068	Phosphorylation	VLLQAFISQLKLEGF HHHHHHHHHHCCCCC	24.01	24719451
1071	Sumoylation	QAFISQLKLEGFALM HHHHHHHCCCCCEEE	36.53	-
1110	2-Hydroxyisobutyrylation	GWAQLTDKTLNLCKM CHHHCCHHHHHHHHH	50.07	-
1125	Phosphorylation	IDKRMWQSMCPLRQF HCHHHHHHHCCHHHH	13.57	23401153
1134	Malonylation	CPLRQFRKLPEEVVK CCHHHHHCCCHHHHH	70.45	26320211
1134	Ubiquitination	CPLRQFRKLPEEVVK CCHHHHHCCCHHHHH	70.45	29967540
1141	Acetylation	KLPEEVVKKIEKKNF CCCHHHHHHHHHCCC	53.89	26051181
1141	Ubiquitination	KLPEEVVKKIEKKNF CCCHHHHHHHHHCCC	53.89	-
1146	2-Hydroxyisobutyrylation	VVKKIEKKNFPFERL HHHHHHHCCCCHHHH	51.54	-
1146	Ubiquitination	VVKKIEKKNFPFERL HHHHHHHCCCCHHHH	51.54	33845483
1154	Phosphorylation	NFPFERLYDLNHNEI CCCHHHHCCCCCCHH	25.30	27642862
1173	Phosphorylation	RMPKMGKTIHKYVHL CCCCCCCCHHHHHHH	23.68	24719451
1176	Acetylation	KMGKTIHKYVHLFPK CCCCCHHHHHHHHHC	45.15	19608861
1176	Malonylation	KMGKTIHKYVHLFPK CCCCCHHHHHHHHHC	45.15	26320211
1176	Ubiquitination	KMGKTIHKYVHLFPK CCCCCHHHHHHHHHC	45.15	19608861
1177	Phosphorylation	MGKTIHKYVHLFPKL CCCCHHHHHHHHHCC	4.48	28152594
1183	Ubiquitination	KYVHLFPKLELSVHL HHHHHHHCCEEEEEE	47.46	33845483
1199	Sumoylation	PITRSTLKVELTITP ECCCCEEEEEEEECC	33.48	-
1238	Phosphorylation	EVILHHEYFLLKAKY HHEEEEEEEHHHHHH	8.65	-
1294	Ubiquitination	RHLILPEKYPPPTEL EEECCCCCCCCCCHH	62.20	33845483
1319	Phosphorylation	LRNSAFESLYQDKFP HHCHHHHHHHCCCCC	26.39	21601212
1321	Phosphorylation	NSAFESLYQDKFPFF CHHHHHHHCCCCCCC	24.76	-
1367	Sulfoxidation	AEFAILRMLLQSSEG HHHHHHHHHHHCCCC	3.82	21406390
1371	Phosphorylation	ILRMLLQSSEGRCVY HHHHHHHCCCCCEEE	30.26	27174698
1372	Phosphorylation	LRMLLQSSEGRCVYI HHHHHHCCCCCEEEE	30.98	27174698
1378	Phosphorylation	SSEGRCVYITPMEAL CCCCCEEEECCHHHH	11.60	27174698
1380	Phosphorylation	EGRCVYITPMEALAE CCCEEEECCHHHHHH	10.01	27174698
1390	Phosphorylation	EALAEQVYMDWYEKF HHHHHHHHHHHHHHH	6.77	27174698
1394	Phosphorylation	EQVYMDWYEKFQDRL HHHHHHHHHHHHHHH	12.65	27174698
1403	Ubiquitination	KFQDRLNKKVVLLTG HHHHHHCCCEEEEEC	52.08	23000965
1404	Ubiquitination	FQDRLNKKVVLLTGE HHHHHCCCEEEEECC	36.18	23000965
1409	Phosphorylation	NKKVVLLTGETSTDL CCCEEEEECCCCCCE	28.89	24670416
1412	Phosphorylation	VVLLTGETSTDLKLL EEEEECCCCCCEEEC	37.97	24670416
1414	Phosphorylation	LLTGETSTDLKLLGK EEECCCCCCEEECCC	53.42	24670416
1417	Acetylation	GETSTDLKLLGKGNI CCCCCCEEECCCCCE	44.46	25953088
1417	Ubiquitination	GETSTDLKLLGKGNI CCCCCCEEECCCCCE	44.46	23000965
1421	2-Hydroxyisobutyrylation	TDLKLLGKGNIIIST CCEEECCCCCEEEEC	49.07	-
1421	Malonylation	TDLKLLGKGNIIIST CCEEECCCCCEEEEC	49.07	26320211
1421	Ubiquitination	TDLKLLGKGNIIIST CCEEECCCCCEEEEC	49.07	23000965
1421 (in isoform 1)	Ubiquitination	-	49.07	21890473
1427	Phosphorylation	GKGNIIISTPEKWDI CCCCEEEECHHHHHH	27.74	27050516
1428	Phosphorylation	KGNIIISTPEKWDIL CCCEEEECHHHHHHH	25.67	30624053
1431	Acetylation	IIISTPEKWDILSRR EEEECHHHHHHHHHH	51.90	26051181
1431	Ubiquitination	IIISTPEKWDILSRR EEEECHHHHHHHHHH	51.90	23000965
1431 (in isoform 1)	Ubiquitination	-	51.90	21890473
1443	Sumoylation	SRRWKQRKNVQNINL HHHHHHCCCCCCEEE	60.00	-
1491	Phosphorylation	PIRIVALSSSLSNAK CCEEEEECCCCCCHH	14.24	21406692
1492	Phosphorylation	IRIVALSSSLSNAKD CEEEEECCCCCCHHH	35.83	21406692
1493	Phosphorylation	RIVALSSSLSNAKDV EEEEECCCCCCHHHH	32.23	21406692
1495	Phosphorylation	VALSSSLSNAKDVAH EEECCCCCCHHHHHH	36.37	21406692
1541	Phosphorylation	HTQTRLLSMAKPVYH HHHHHHHHHHHHHHH	22.68	-
1544	Acetylation	TRLLSMAKPVYHAIT HHHHHHHHHHHHHHH	26.78	26051181
1544	Ubiquitination	TRLLSMAKPVYHAIT HHHHHHHHHHHHHHH	26.78	23000965
1544 (in isoform 1)	Ubiquitination	-	26.78	21890473
1552	Acetylation	PVYHAITKHSPKKPV HHHHHHHCCCCCCCE	35.65	25953088
1552	Ubiquitination	PVYHAITKHSPKKPV HHHHHHHCCCCCCCE	35.65	23000965
1556	Ubiquitination	AITKHSPKKPVIVFV HHHCCCCCCCEEEEE	73.12	23000965
1557	Ubiquitination	ITKHSPKKPVIVFVP HHCCCCCCCEEEEEC	48.15	23000965
1569	Phosphorylation	FVPSRKQTRLTAIDI EECCCCCCHHHHHHH	30.59	24719451
1572	Phosphorylation	SRKQTRLTAIDILTT CCCCCHHHHHHHHHH	20.48	-
1579	Phosphorylation	TAIDILTTCAADIQR HHHHHHHHHHHHHHH	9.19	24719451
1580	Glutathionylation	AIDILTTCAADIQRQ HHHHHHHHHHHHHHH	2.18	22555962
1593	Phosphorylation	RQRFLHCTEKDLIPY HHHHHCCCHHHHHHH	35.01	24719451
1595	Acetylation	RFLHCTEKDLIPYLE HHHCCCHHHHHHHHH	39.42	25825284
1595	Ubiquitination	RFLHCTEKDLIPYLE HHHCCCHHHHHHHHH	39.42	23000965
1600	Phosphorylation	TEKDLIPYLEKLSDS CHHHHHHHHHHCCCC	22.09	29083192
1603	2-Hydroxyisobutyrylation	DLIPYLEKLSDSTLK HHHHHHHHCCCCHHH	51.17	-
1603	Ubiquitination	DLIPYLEKLSDSTLK HHHHHHHHCCCCHHH	51.17	21906983
1603 (in isoform 1)	Ubiquitination	-	51.17	21890473
1605	Phosphorylation	IPYLEKLSDSTLKET HHHHHHCCCCHHHHH	40.35	27251275
1607	Phosphorylation	YLEKLSDSTLKETLL HHHHCCCCHHHHHHH	32.06	30266825
1608	Phosphorylation	LEKLSDSTLKETLLN HHHCCCCHHHHHHHH	47.00	30266825
1625	Phosphorylation	GYLHEGLSPMERRLV CHHCCCCCHHHHHHH	32.81	-
1637	Phosphorylation	RLVEQLFSSGAIQVV HHHHHHHCCCCCEEE	36.13	29978859
1638	Phosphorylation	LVEQLFSSGAIQVVV HHHHHHCCCCCEEEE	25.55	29978859
1647	Phosphorylation	AIQVVVASRSLCWGM CCEEEEECCCHHHCC	15.70	29978859
1648	Dimethylation	IQVVVASRSLCWGMN CEEEEECCCHHHCCC	24.74	-
1648	Methylation	IQVVVASRSLCWGMN CEEEEECCCHHHCCC	24.74	115390075
1679	Phosphorylation	KIHAYVDYPIYDVLQ EEEEEEECCHHHHHH	4.98	-
1682	Phosphorylation	AYVDYPIYDVLQMVG EEEECCHHHHHHHHC	8.76	28064214
1711	Ubiquitination	IMCQGSKKDFFKKFL EEECCCCHHHHHHHC	62.56	-
1743	Ubiquitination	FNAEIVTKTIENKQD CCCEEHHHHHCCCHH	36.29	22817900
1748	Ubiquitination	VTKTIENKQDAVDYL HHHHHCCCHHHHHHH	36.44	21963094
1754	Phosphorylation	NKQDAVDYLTWTFLY CCHHHHHHHHHHHHH	10.22	22817900
1765	Phosphorylation	TFLYRRMTQNPNYYN HHHHHHHCCCCCCCC	24.31	24043423
1770	Phosphorylation	RMTQNPNYYNLQGIS HHCCCCCCCCCCCCC	8.59	17360941
1771	Phosphorylation	MTQNPNYYNLQGISH HCCCCCCCCCCCCCH	18.94	24043423
1777	Phosphorylation	YYNLQGISHRHLSDH CCCCCCCCHHHHHHH	22.69	24043423
1782	Phosphorylation	GISHRHLSDHLSELV CCCHHHHHHHHHHHH	19.50	28450419
1786	Phosphorylation	RHLSDHLSELVEQTL HHHHHHHHHHHHHHH	26.01	28450419
1792	Phosphorylation	LSELVEQTLSDLEQS HHHHHHHHHHHHHHH	18.09	28122231
1794	Phosphorylation	ELVEQTLSDLEQSKC HHHHHHHHHHHHHCC	43.46	28122231
1855	Phosphorylation	IISNAAEYENIPIRH HHHCCHHHCCCCCCC	15.00	-
1874	Acetylation	LLRQLAQKVPHKLNN HHHHHHHHCCHHHCC	52.72	25953088
1874	Ubiquitination	LLRQLAQKVPHKLNN HHHHHHHHCCHHHCC	52.72	29967540
1878	Acetylation	LAQKVPHKLNNPKFN HHHHCCHHHCCCCCC	47.00	25953088
1878	Ubiquitination	LAQKVPHKLNNPKFN HHHHCCHHHCCCCCC	47.00	29967540
1883	Acetylation	PHKLNNPKFNDPHVK CHHHCCCCCCCHHHH	59.95	25953088
1883	Ubiquitination	PHKLNNPKFNDPHVK CHHHCCCCCCCHHHH	59.95	29967540
1890	Ubiquitination	KFNDPHVKTNLLLQA CCCCHHHHHHHHHHH	28.76	33845483
1891	Phosphorylation	FNDPHVKTNLLLQAH CCCHHHHHHHHHHHH	29.97	21406692
1900	Phosphorylation	LLLQAHLSRMQLSAE HHHHHHHHHHHHHHH	18.81	21406692
1910	Phosphorylation	QLSAELQSDTEEILS HHHHHHCCCHHHHHH	60.15	-
1918	Ubiquitination	DTEEILSKAIRLIQA CHHHHHHHHHHHHHH	44.37	33845483
1931	Phosphorylation	QACVDVLSSNGWLSP HHHHHHHHCCCCCCH	22.93	22210691
1932	Phosphorylation	ACVDVLSSNGWLSPA HHHHHHHCCCCCCHH	34.89	22210691
1937	Phosphorylation	LSSNGWLSPALAAME HHCCCCCCHHHHHHH	11.13	22210691
1955	Phosphorylation	MVTQAMWSKDSYLKQ HHHHHHHCCCHHHHH	17.32	-
1956	Ubiquitination	VTQAMWSKDSYLKQL HHHHHHCCCHHHHHC	34.87	22817900
1958	Phosphorylation	QAMWSKDSYLKQLPH HHHHCCCHHHHHCCC	35.78	-
1959	Phosphorylation	AMWSKDSYLKQLPHF HHHCCCHHHHHCCCC	28.22	-
1961	Ubiquitination	WSKDSYLKQLPHFTS HCCCHHHHHCCCCCH	41.37	22817900
1961 (in isoform 1)	Ubiquitination	-	41.37	21890473
1972	Ubiquitination	HFTSEHIKRCTDKGV CCCHHHHHHHCCCCC	43.24	33845483
2000	Phosphorylation	RNALLQLTDSQIADV HHHHHHCCHHHHHHH	22.20	30266825
2002	Phosphorylation	ALLQLTDSQIADVAR HHHHCCHHHHHHHHH	20.46	30266825
2020	Phosphorylation	RYPNIELSYEVVDKD CCCCEEEEEEEECHH	13.37	-
2021	Phosphorylation	YPNIELSYEVVDKDS CCCEEEEEEEECHHH	25.93	17360941
2059	Ubiquitination	IAPLFPQKREEGWWV EECCCCCCCCCCEEE	63.11	21906983
2059 (in isoform 1)	Ubiquitination	-	63.11	21890473
2075	Phosphorylation	IGDAKSNSLISIKRL EEECCCCCEEEEEEE	33.88	21210654
2080	2-Hydroxyisobutyrylation	SNSLISIKRLTLQQK CCCEEEEEEEECHHC	34.27	-
2080	Acetylation	SNSLISIKRLTLQQK CCCEEEEEEEECHHC	34.27	25953088
2080	Ubiquitination	SNSLISIKRLTLQQK CCCEEEEEEEECHHC	34.27	21906983
2080 (in isoform 1)	Ubiquitination	-	34.27	21890473
2087	Acetylation	KRLTLQQKAKVKLDF EEEECHHCCCEEEEE	36.89	25953088
2087	Ubiquitination	KRLTLQQKAKVKLDF EEEECHHCCCEEEEE	36.89	32142685
2091	Sumoylation	LQQKAKVKLDFVAPA CHHCCCEEEEEEECC	41.45	-
2123	Phosphorylation	CDQEYKFSVDVKEAE CCCEEEEEEEHHHCC	17.16	28634120
2131	Phosphorylation	VDVKEAETDSDSD-- EEHHHCCCCCCCC--	48.10	26503892
2133	Phosphorylation	VKEAETDSDSD---- HHHCCCCCCCC----	46.57	26503892
2135	Phosphorylation	EAETDSDSD------ HCCCCCCCC------	49.53	26503892

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of U520_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of U520_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of U520_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
U5S1_HUMAN	EFTUD2	physical	16723661
U520_HUMAN	SNRNP200	physical	16723661
PRP8_HUMAN	PRPF8	physical	16723661
SNR40_HUMAN	SNRNP40	physical	16723661
U5S1_HUMAN	EFTUD2	physical	22939629
YBOX1_HUMAN	YBX1	physical	22939629
VIR_HUMAN	KIAA1429	physical	22939629
YETS2_HUMAN	YEATS2	physical	22939629
UBR4_HUMAN	UBR4	physical	22939629
ZBT10_HUMAN	ZBTB10	physical	22939629
USP9X_HUMAN	USP9X	physical	22939629
SF3B2_HUMAN	SF3B2	physical	22365833
U520_HUMAN	SNRNP200	physical	22365833
LSM8_HUMAN	LSM8	physical	22365833
R113A_HUMAN	RNF113A	physical	22365833
WASC4_HUMAN	KIAA1033	physical	22863883
RPAB3_HUMAN	POLR2H	physical	22863883
T22D1_HUMAN	TSC22D1	physical	22863883
UBP34_HUMAN	USP34	physical	22863883
WDHD1_HUMAN	WDHD1	physical	22863883
PRP8_HUMAN	PRPF8	physical	23704370
PRP8_HUMAN	PRPF8	physical	17317632
BPTF_HUMAN	BPTF	physical	26344197
CD2B2_HUMAN	CD2BP2	physical	26344197
U5S1_HUMAN	EFTUD2	physical	26344197
RBP2_HUMAN	RANBP2	physical	26344197
SNUT1_HUMAN	SART1	physical	26344197
SF3A3_HUMAN	SF3A3	physical	26344197
SMD1_HUMAN	SNRPD1	physical	26344197
RUXE_HUMAN	SNRPE	physical	26344197
SRRM2_HUMAN	SRRM2	physical	26344197
SMU1_HUMAN	SMU1	physical	27173435

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 610359: Retinitis pigmentosa 33 (RP33)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.

Related Literatures of Post-Translational Modification

Acetylation

"Lysine acetylation targets protein complexes and co-regulates majorcellular functions.";
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.,Olsen J.V., Mann M.;
Science 325:834-840(2009).
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-469; LYS-971 AND LYS-1176,AND MASS SPECTROMETRY.
PubMed: 19608861

Phosphorylation

"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions.";
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.;
Sci. Signal. 2:RA46-RA46(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225; THR-2131; SER-2133AND SER-2135, AND MASS SPECTROMETRY.
PubMed: 19690332

"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach.";
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.;
Anal. Chem. 81:4493-4501(2009).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND MASSSPECTROMETRY.
PubMed: 19413330

"Large-scale phosphoproteome analysis of human liver tissue byenrichment and fractionation of phosphopeptides with strong anionexchange chromatography.";
Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D.,Zou H., Gu J.;
Proteomics 8:1346-1361(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND MASSSPECTROMETRY.
PubMed: 18318008

"A quantitative atlas of mitotic phosphorylation.";
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-225; THR-2131;SER-2133 AND SER-2135, AND MASS SPECTROMETRY.
PubMed: 18669648

"Kinase-selective enrichment enables quantitative phosphoproteomics ofthe kinome across the cell cycle.";
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,Greff Z., Keri G., Stemmann O., Mann M.;
Mol. Cell 31:438-448(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND MASSSPECTROMETRY.
PubMed: 18691976

"Phosphorylation analysis of primary human T lymphocytes usingsequential IMAC and titanium oxide enrichment.";
Carrascal M., Ovelleiro D., Casas V., Gay M., Abian J.;
J. Proteome Res. 7:5167-5176(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND MASSSPECTROMETRY.
PubMed: 19367720

"Evaluation of the low-specificity protease elastase for large-scalephosphoproteome analysis.";
Wang B., Malik R., Nigg E.A., Korner R.;
Anal. Chem. 80:9526-9533(2008).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND MASSSPECTROMETRY.
PubMed: 19007248

"Phosphoproteome analysis of the human mitotic spindle.";
Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.;
Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225, AND MASSSPECTROMETRY.
PubMed: 16565220

"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks.";
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.;
Cell 127:635-648(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225 AND SER-1056, ANDMASS SPECTROMETRY.
PubMed: 17081983

"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J.,Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-225 AND SER-756, ANDMASS SPECTROMETRY.
PubMed: 15302935

"Tyrosine phosphorylated Par3 regulates epithelial tight junctionassembly promoted by EGFR signaling.";
Wang Y., Du D., Fang L., Yang G., Zhang C., Zeng R., Ullrich A.,Lottspeich F., Chen Z.;
EMBO J. 25:5058-5070(2006).
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-922; TYR-924 ANDTYR-926, AND MASS SPECTROMETRY.
PubMed: 17053785

Sumoylation

"A proteomic study of SUMO-2 target proteins.";
Vertegaal A.C.O., Ogg S.C., Jaffray E., Rodriguez M.S., Hay R.T.,Andersen J.S., Mann M., Lamond A.I.;
J. Biol. Chem. 279:33791-33798(2004).
Cited for: SUMOYLATION [LARGE SCALE ANALYSIS] AT LYS-944; LYS-971; LYS-1071;LYS-1199 AND LYS-2091, AND MASS SPECTROMETRY.
PubMed: 15175327