dbPTM

HAUS8_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	HAUS8_HUMAN
UniProt AC	Q9BT25
Protein Name	HAUS augmin-like complex subunit 8
Gene Name	HAUS8
Organism	Homo sapiens (Human).
Sequence Length	410
Subcellular Localization	Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . During interphase, primarily cytoplasmic and associates with centrosomes and with the mitotic
Protein Description	Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex..
Protein Sequence	MADSSGRGAGKPATGPTNSSSAKKKDKRVQGGRVIESRYLQYEKKTTQKAPAGDGSQTRGKMSEGGRKSSLLQKSKADSSGVGKGDLQSTLLEGHGTAPPDLDLSAINDKSIVKKTPQLAKTISKKPESTSFSAPRKKSPDLSEAMEMMESQTLLLTLLSVKMENNLAEFERRAEKNLLIMCKEKEKLQKKAHELKRRLLLSQRKRELADVLDAQIEMLSPFEAVATRFKEQYRTFATALDTTRHELPVRSIHLEGDGQQLLDALQHELVTTQRLLGELDVGDSEENVQVLDLLSELKDVTAKKDLELRRSFAQVLELSAEASKEAALANQEVWEETQGMAPPSRWYFNQDSACRESGGAPKNTPLSEDDNPGASSAPAQATFISPSEDFSSSSQAEVPPSLSRSGRDLS

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	Acetylation	------MADSSGRGA ------CCCCCCCCC	25.95	19413330
5	Phosphorylation	---MADSSGRGAGKP ---CCCCCCCCCCCC	32.81	26074081
7	Methylation	-MADSSGRGAGKPAT -CCCCCCCCCCCCCC	33.46	-
9 (in isoform 2)	Phosphorylation	-	22.83	29743597
11	Ubiquitination	SSGRGAGKPATGPTN CCCCCCCCCCCCCCC	30.83	24816145
14	Phosphorylation	RGAGKPATGPTNSSS CCCCCCCCCCCCCCC	52.82	26074081
17	Phosphorylation	GKPATGPTNSSSAKK CCCCCCCCCCCCCCH	49.42	29396449
19	Phosphorylation	PATGPTNSSSAKKKD CCCCCCCCCCCCHHC	28.31	25262027
20	Phosphorylation	ATGPTNSSSAKKKDK CCCCCCCCCCCHHCC	36.06	25159151
21	Phosphorylation	TGPTNSSSAKKKDKR CCCCCCCCCCHHCCC	43.88	25159151
39	Phosphorylation	GRVIESRYLQYEKKT CCEEHHHEEEEECCC	13.87	29083192
42	Phosphorylation	IESRYLQYEKKTTQK EHHHEEEEECCCCCC	27.41	22817900
46	Phosphorylation	YLQYEKKTTQKAPAG EEEEECCCCCCCCCC	45.59	29083192
47	Phosphorylation	LQYEKKTTQKAPAGD EEEECCCCCCCCCCC	36.63	29083192
49	Acetylation	YEKKTTQKAPAGDGS EECCCCCCCCCCCCC	55.00	25953088
49	Ubiquitination	YEKKTTQKAPAGDGS EECCCCCCCCCCCCC	55.00	24816145
56	Phosphorylation	KAPAGDGSQTRGKMS CCCCCCCCCCCCCCC	32.87	-
59	Dimethylation	AGDGSQTRGKMSEGG CCCCCCCCCCCCCCC	34.79	-
59	Methylation	AGDGSQTRGKMSEGG CCCCCCCCCCCCCCC	34.79	-
61	"N6,N6-dimethyllysine"	DGSQTRGKMSEGGRK CCCCCCCCCCCCCHH	35.64	-
61	Methylation	DGSQTRGKMSEGGRK CCCCCCCCCCCCCHH	35.64	-
67	Dimethylation	GKMSEGGRKSSLLQK CCCCCCCHHHHHHHH	46.68	-
67	Methylation	GKMSEGGRKSSLLQK CCCCCCCHHHHHHHH	46.68	-
69	Phosphorylation	MSEGGRKSSLLQKSK CCCCCHHHHHHHHHC	25.51	26074081
70	Phosphorylation	SEGGRKSSLLQKSKA CCCCHHHHHHHHHCC	35.81	23911959
74	Ubiquitination	RKSSLLQKSKADSSG HHHHHHHHHCCCCCC	54.62	29967540
79	Phosphorylation	LQKSKADSSGVGKGD HHHHCCCCCCCCCCH	33.57	20068231
89	Phosphorylation	VGKGDLQSTLLEGHG CCCCHHHHHHHCCCC	28.46	18691976
90	Phosphorylation	GKGDLQSTLLEGHGT CCCHHHHHHHCCCCC	23.54	29978859
97	Phosphorylation	TLLEGHGTAPPDLDL HHHCCCCCCCCCCCH	30.25	27732954
105	Phosphorylation	APPDLDLSAINDKSI CCCCCCHHHHCCHHH	27.11	25159151
109	Ubiquitination	LDLSAINDKSIVKKT CCHHHHCCHHHHHHC	39.71	29967540
110	Ubiquitination	DLSAINDKSIVKKTP CHHHHCCHHHHHHCH	37.28	29967540
111	Phosphorylation	LSAINDKSIVKKTPQ HHHHCCHHHHHHCHH	35.16	28555341
114	Ubiquitination	INDKSIVKKTPQLAK HCCHHHHHHCHHHHH	49.62	29967540
115	Ubiquitination	NDKSIVKKTPQLAKT CCHHHHHHCHHHHHH	55.32	29967540
116	Phosphorylation	DKSIVKKTPQLAKTI CHHHHHHCHHHHHHH	15.56	21712546
122	Phosphorylation	KTPQLAKTISKKPES HCHHHHHHHCCCCCC	25.52	21690413
124	Ubiquitination	PQLAKTISKKPESTS HHHHHHHCCCCCCCC	39.60	29967540
124	Phosphorylation	PQLAKTISKKPESTS HHHHHHHCCCCCCCC	39.60	26657352
125	Ubiquitination	QLAKTISKKPESTSF HHHHHHCCCCCCCCC	69.38	29967540
126	Ubiquitination	LAKTISKKPESTSFS HHHHHCCCCCCCCCC	47.42	29967540
129	Phosphorylation	TISKKPESTSFSAPR HHCCCCCCCCCCCCC	37.57	28450419
130	Phosphorylation	ISKKPESTSFSAPRK HCCCCCCCCCCCCCC	31.93	28450419
131	Phosphorylation	SKKPESTSFSAPRKK CCCCCCCCCCCCCCC	26.87	25159151
133	Phosphorylation	KPESTSFSAPRKKSP CCCCCCCCCCCCCCC	36.81	26657352
139	Phosphorylation	FSAPRKKSPDLSEAM CCCCCCCCCCHHHHH	27.04	25159151
143	Phosphorylation	RKKSPDLSEAMEMME CCCCCCHHHHHHHHH	30.59	21690413
151	Phosphorylation	EAMEMMESQTLLLTL HHHHHHHHHHHHHHH	15.57	23917254
153	Phosphorylation	MEMMESQTLLLTLLS HHHHHHHHHHHHHHH	28.75	-
157	Phosphorylation	ESQTLLLTLLSVKME HHHHHHHHHHHHHHH	26.14	24719451
160	Phosphorylation	TLLLTLLSVKMENNL HHHHHHHHHHHHCCH	23.86	24719451
175	Ubiquitination	AEFERRAEKNLLIMC HHHHHHHHHHEEHHH	40.44	29967540
176	Ubiquitination	EFERRAEKNLLIMCK HHHHHHHHHEEHHHC	52.49	29967540
202	Phosphorylation	LKRRLLLSQRKRELA HHHHHHHHHHHHHHH	28.10	24719451
220	Phosphorylation	DAQIEMLSPFEAVAT HHHHHHCCHHHHHHH	26.66	21690413
230	Ubiquitination	EAVATRFKEQYRTFA HHHHHHHHHHHHHHH	40.39	-
235	Phosphorylation	RFKEQYRTFATALDT HHHHHHHHHHHHHHC	16.82	21690413
238	Phosphorylation	EQYRTFATALDTTRH HHHHHHHHHHHCCCC	24.46	21690413
311	Phosphorylation	KDLELRRSFAQVLEL HCHHHHHHHHHHHHH	20.64	21690413
319	Phosphorylation	FAQVLELSAEASKEA HHHHHHHCHHHHHHH	18.06	28555341
347	Phosphorylation	MAPPSRWYFNQDSAC CCCCHHHCCCCCHHH	7.49	27642862
352	Phosphorylation	RWYFNQDSACRESGG HHCCCCCHHHHHCCC	21.36	24719451
357	Phosphorylation	QDSACRESGGAPKNT CCHHHHHCCCCCCCC	24.15	25159151
382	Phosphorylation	SSAPAQATFISPSED CCCCCEEEEECCCCC	14.93	25627689
385	Phosphorylation	PAQATFISPSEDFSS CCEEEEECCCCCCCC	20.41	25627689

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
19	S	Phosphorylation	Kinase	AURA	O14965	PSP
20	S	Phosphorylation	Kinase	AURA	O14965	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of HAUS8_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of HAUS8_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
HAUS6_HUMAN	HAUS6	physical	19369198
HAUS5_HUMAN	HAUS5	physical	19369198
A4_HUMAN	APP	physical	21832049
SEC20_HUMAN	BNIP1	physical	28514442
RBP10_HUMAN	RANBP10	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of HAUS8_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."; Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.; J. Proteome Res. 6:4150-4162(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, AND MASSSPECTROMETRY.	17924679 [Abstract]