SEC20_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: SEC20_HUMAN
• UniProt AC: Q12981
• Protein Name: Vesicle transport protein SEC20
• Gene Name: BNIP1
• Organism: Homo sapiens (Human).
• Sequence Length: 228
• Subcellular Localization: Mitochondrion. Endoplasmic reticulum membrane; Single-pass type IV membrane protein.
• Protein Description: SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy..
• Protein Sequence: MAAPQDVHVRICNQEIVKFDLEVKALIQDIRDCSGPLSALTELNTKVKEKFQQLRHRIQDLEQLAKEQDKESEKQLLLQEVENHKKQMLSNQASWRKANLTCKIAIDNLEKAELLQGGDLLRQRKTTKESLAQTSSTITESLMGISRMMAQQVQQSEEAMQSLVTSSRTILDANEEFKSMSGTIQLGRKLITKYNRRELTDKLLIFLALALFLATVLYIVKKRLFPFL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
46	Acetylation	ALTELNTKVKEKFQQ HHHHHHHHHHHHHHH	50.68	20167786
46	Ubiquitination	ALTELNTKVKEKFQQ HHHHHHHHHHHHHHH	50.68	32015554
48	Ubiquitination	TELNTKVKEKFQQLR HHHHHHHHHHHHHHH	57.66	-
48	Acetylation	TELNTKVKEKFQQLR HHHHHHHHHHHHHHH	57.66	20167786
48	Methylation	TELNTKVKEKFQQLR HHHHHHHHHHHHHHH	57.66	23644510
48 (in isoform 1)	Ubiquitination	-	57.66	-
48	"N6,N6-dimethyllysine"	TELNTKVKEKFQQLR HHHHHHHHHHHHHHH	57.66	-
50	"N6,N6-dimethyllysine"	LNTKVKEKFQQLRHR HHHHHHHHHHHHHHH	42.63	-
50	Ubiquitination	LNTKVKEKFQQLRHR HHHHHHHHHHHHHHH	42.63	29967540
50	Methylation	LNTKVKEKFQQLRHR HHHHHHHHHHHHHHH	42.63	23644510
66	Ubiquitination	QDLEQLAKEQDKESE HHHHHHHHHCCHHHH	65.58	29967540
74	Ubiquitination	EQDKESEKQLLLQEV HCCHHHHHHHHHHHH	56.37	29967540
86	Ubiquitination	QEVENHKKQMLSNQA HHHHHHHHHHHHCHH	33.13	29967540
90	Phosphorylation	NHKKQMLSNQASWRK HHHHHHHHCHHHHHH	22.89	-
92 (in isoform 2)	Phosphorylation	-	33.14	21060948
94	Phosphorylation	QMLSNQASWRKANLT HHHHCHHHHHHCCCH	20.04	-
94	Ubiquitination	QMLSNQASWRKANLT HHHHCHHHHHHCCCH	20.04	32015554
94 (in isoform 2)	Ubiquitination	-	20.04	21906983
103	Ubiquitination	RKANLTCKIAIDNLE HHCCCHHEEEECCHH	29.91	32015554
107 (in isoform 2)	Phosphorylation	-	44.64	22210691
111	Ubiquitination	IAIDNLEKAELLQGG EEECCHHHHHHHCCH	50.78	23000965
111 (in isoform 4)	Ubiquitination	-	50.78	21906983
125	Ubiquitination	GDLLRQRKTTKESLA HHHHHHCCCHHHHHH	52.81	23000965
126	Phosphorylation	DLLRQRKTTKESLAQ HHHHHCCCHHHHHHH	45.34	-
127	Phosphorylation	LLRQRKTTKESLAQT HHHHCCCHHHHHHHH	35.53	-
128	Ubiquitination	LRQRKTTKESLAQTS HHHCCCHHHHHHHHH	51.00	23000965
128 (in isoform 4)	Ubiquitination	-	51.00	21906983
129	Ubiquitination	RQRKTTKESLAQTSS HHCCCHHHHHHHHHH	49.22	29967540
135 (in isoform 3)	Phosphorylation	-	17.26	21060948
137	Ubiquitination	SLAQTSSTITESLMG HHHHHHHHHHHHHHH	31.52	32015554
137 (in isoform 3)	Ubiquitination	-	31.52	21906983
144	Ubiquitination	TITESLMGISRMMAQ HHHHHHHHHHHHHHH	21.73	22817900
144 (in isoform 2)	Ubiquitination	-	21.73	21906983
146	Ubiquitination	TESLMGISRMMAQQV HHHHHHHHHHHHHHH	14.37	32015554
150 (in isoform 3)	Phosphorylation	-	6.31	22210691
154 (in isoform 1)	Ubiquitination	-	36.42	21906983
154	Ubiquitination	RMMAQQVQQSEEAMQ HHHHHHHHHCHHHHH	36.42	23000965
162	Phosphorylation	QSEEAMQSLVTSSRT HCHHHHHHHHHHHCC	16.24	28555341
165	Phosphorylation	EAMQSLVTSSRTILD HHHHHHHHHHCCHHH	26.69	27762562
168	Ubiquitination	QSLVTSSRTILDANE HHHHHHHCCHHHCCH	26.08	23000965
171 (in isoform 1)	Ubiquitination	-	5.14	21906983
171	Ubiquitination	VTSSRTILDANEEFK HHHHCCHHHCCHHHH	5.14	23000965
178	Ubiquitination	LDANEEFKSMSGTIQ HHCCHHHHHCCCHHH	48.83	22817900
178 (in isoform 4)	Ubiquitination	-	48.83	21906983
179	Phosphorylation	DANEEFKSMSGTIQL HCCHHHHHCCCHHHH	24.41	23312004
180	Sulfoxidation	ANEEFKSMSGTIQLG CCHHHHHCCCHHHHH	4.40	21406390
181	Phosphorylation	NEEFKSMSGTIQLGR CHHHHHCCCHHHHHH	39.62	23312004
183	Phosphorylation	EFKSMSGTIQLGRKL HHHHCCCHHHHHHHH	9.76	23312004
187	Ubiquitination	MSGTIQLGRKLITKY CCCHHHHHHHHHHHC	14.33	22817900
187 (in isoform 3)	Ubiquitination	-	14.33	21906983
189	Acetylation	GTIQLGRKLITKYNR CHHHHHHHHHHHCCH	42.30	7365071
193	Acetylation	LGRKLITKYNRRELT HHHHHHHHCCHHHHH	33.27	7365083
200	Phosphorylation	KYNRRELTDKLLIFL HCCHHHHHHHHHHHH	26.74	-
215	Phosphorylation	ALALFLATVLYIVKK HHHHHHHHHHHHHHH	17.12	-
218	Phosphorylation	LFLATVLYIVKKRLF HHHHHHHHHHHHHHC	10.18	-
221	Ubiquitination	ATVLYIVKKRLFPFL HHHHHHHHHHHCCCC	23.71	22817900
221 (in isoform 1)	Ubiquitination	-	23.71	21906983

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	RNF185	Q96GF1	PMID:21931693

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
29	K	ubiquitylation	Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.	23896122

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of SEC20_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BCL2_HUMAN	BCL2	physical	10217402
B2CL1_HUMAN	BCL2L1	physical	10217402
RN185_HUMAN	RNF185	physical	21931693
SQSTM_HUMAN	SQSTM1	physical	21931693
A4_HUMAN	APP	physical	21832049
RN186_HUMAN	RNF186	physical	23896122

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.