MIB1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MIB1_HUMAN
• UniProt AC: Q86YT6
• Protein Name: E3 ubiquitin-protein ligase MIB1
• Gene Name: MIB1
• Organism: Homo sapiens (Human).
• Sequence Length: 1006
• Subcellular Localization: Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite . Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remai
• Protein Description: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis (By similarity). Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation..
• Protein Sequence: MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCANLMKKCVQCRAVVERRVPFIMCCGGKSSEDATDDISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
25	Ubiquitination	VVRGPDWKWGKQDGG EEECCCCCCCCCCCC	54.15	23000965
28	Neddylation	GPDWKWGKQDGGEGH CCCCCCCCCCCCCCC	44.06	32015554
28	Ubiquitination	GPDWKWGKQDGGEGH CCCCCCCCCCCCCCC	44.06	21906983
44	Ubiquitination	GTVRSFESPEEVVVV EEEEECCCCCCEEEE	35.28	23503661
50	Ubiquitination	ESPEEVVVVWDNGTA CCCCCEEEEECCCCE	4.32	21890473
76	Phosphorylation	RILDSAPTGIKHDGT EEEECCCCCCCCCCC	51.78	29396449
79	Ubiquitination	DSAPTGIKHDGTMCD ECCCCCCCCCCCCCC	36.67	22817900
106	Phosphorylation	KCAECTNYDLCTVCY EECCCCCCEEEEEEE	7.67	-
128	Ubiquitination	RHRFYRITTPGSERV EECEEEECCCCHHHH	20.24	21890473
132	Phosphorylation	YRITTPGSERVLLES EEECCCCHHHHHCCH	24.35	24719451
141	Ubiquitination	RVLLESRRKSKKITA HHHCCHHHCCCCCCH	57.80	21890473
194	Phosphorylation	SSPHSAAYVLWDNGA CCCCCEEEEEECCCC	8.87	-
196	Ubiquitination	PHSAAYVLWDNGAKN CCCEEEEEECCCCCC	2.87	21890473
201	Ubiquitination	YVLWDNGAKNLYRVG EEEECCCCCCEEEEC	12.10	22053931
202	Acetylation	VLWDNGAKNLYRVGF EEECCCCCCEEEECC	49.21	19822263
202	Ubiquitination	VLWDNGAKNLYRVGF EEECCCCCCEEEECC	49.21	23000965
216	Acetylation	FEGMSDLKCVQDAKG CCCCCCCEEEEECCC	37.03	19822271
216	Ubiquitination	FEGMSDLKCVQDAKG CCCCCCCEEEEECCC	37.03	23503661
222	Neddylation	LKCVQDAKGGSFYRD CEEEEECCCCCCCCC	72.58	32015554
222	Ubiquitination	LKCVQDAKGGSFYRD CEEEEECCCCCCCCC	72.58	22817900
227	Ubiquitination	DAKGGSFYRDHCPVL ECCCCCCCCCCCCCC	19.48	21890473
236	Ubiquitination	DHCPVLGEQNGNRNP CCCCCCCCCCCCCCC	36.39	21890473
245	Ubiquitination	NGNRNPGGLQIGDLV CCCCCCCCCCHHHEE	18.70	21890473
248	Ubiquitination	RNPGGLQIGDLVNID CCCCCCCHHHEEEEE	5.80	23000965
249	Ubiquitination	NPGGLQIGDLVNIDL CCCCCCHHHEEEEEC	14.83	23000965
259	Ubiquitination	VNIDLDLEIVQSLQH EEEECCHHHHHHHHC	40.56	21890473
260	Ubiquitination	NIDLDLEIVQSLQHG EEECCHHHHHHHHCC	4.43	22817900
265	Ubiquitination	LEIVQSLQHGHGGWT HHHHHHHHCCCCCCC	45.85	21890473
281	Ubiquitination	GMFETLTTTGTVCGI CCEEEECCCCCEECC	26.75	22505724
282	Ubiquitination	MFETLTTTGTVCGID CEEEECCCCCEECCC	26.14	22505724
297	Ubiquitination	EDHDIVVQYPSGNRW CCCCEEEECCCCCCE	33.26	21890473
301	Ubiquitination	IVVQYPSGNRWTFNP EEEECCCCCCEEECC	24.18	22817900
304	Ubiquitination	QYPSGNRWTFNPAVL ECCCCCCEEECCHHH	14.89	22817900
306	Ubiquitination	PSGNRWTFNPAVLTK CCCCCEEECCHHHEC	9.05	22053931
309	Ubiquitination	NRWTFNPAVLTKANI CCEEECCHHHECCCE	15.28	22817900
313	Ubiquitination	FNPAVLTKANIVRSG ECCHHHECCCEEECC	34.96	21963094
323	Ubiquitination	IVRSGDAAQGAEGGT EEECCCCCCCCCCCC	17.06	22817900
336	Ubiquitination	GTSQFQVGDLVQVCY CCCCCCHHHHHHHHH	16.26	22817900
349	Ubiquitination	CYDLERIKLLQRGHG HHCHHHHHHHHCCCH	49.74	32015554
358	Ubiquitination	LQRGHGEWAEAMLPT HHCCCHHHHHHHHHH	12.19	23503661
359	Ubiquitination	QRGHGEWAEAMLPTL HCCCHHHHHHHHHHC	7.14	23503661
364	Ubiquitination	EWAEAMLPTLGKVGR HHHHHHHHHCCCCCC	16.70	23503661
368	Ubiquitination	AMLPTLGKVGRVQQI HHHHHCCCCCCEEEE	44.63	21906983
376	Phosphorylation	VGRVQQIYSDSDLKV CCCEEEEECCCCCEE	11.33	25884760
377	Phosphorylation	GRVQQIYSDSDLKVE CCEEEEECCCCCEEE	32.06	21815630
382	Ubiquitination	IYSDSDLKVEVCGTS EECCCCCEEEEECCC	41.14	22817900
383	Ubiquitination	YSDSDLKVEVCGTSW ECCCCCEEEEECCCC	9.66	22817900
399	Ubiquitination	YNPAAVSKVASAGSA CCHHHHHHHHHCCHH	34.91	21906983
402	Phosphorylation	AAVSKVASAGSAISN HHHHHHHHCCHHHHC	35.83	23403867
405	Phosphorylation	SKVASAGSAISNASG HHHHHCCHHHHCCCH	23.34	30266825
405	Ubiquitination	SKVASAGSAISNASG HHHHHCCHHHHCCCH	23.34	21963094
406	Ubiquitination	KVASAGSAISNASGE HHHHCCHHHHCCCHH	14.33	22817900
408	Phosphorylation	ASAGSAISNASGERL HHCCHHHHCCCHHHH	26.51	30266825
411	Phosphorylation	GSAISNASGERLSQL CHHHHCCCHHHHHHH	45.50	30266825
416	Phosphorylation	NASGERLSQLLKKLF CCCHHHHHHHHHHHH	25.63	30266825
420	Ubiquitination	ERLSQLLKKLFETQE HHHHHHHHHHHHCCC	56.90	23000965
421	Ubiquitination	RLSQLLKKLFETQES HHHHHHHHHHHCCCC	59.38	23000965
427	Ubiquitination	KKLFETQESGDLNEE HHHHHCCCCCCCCHH	65.20	23503661
437	Ubiquitination	DLNEELVKAAANGDV CCCHHHHHHHHCCCC	44.95	21906983
446	Ubiquitination	AANGDVAKVEDLLKR HHCCCCCCHHHHHHC	46.11	27667366
448	Ubiquitination	NGDVAKVEDLLKRPD CCCCCCHHHHHHCCC	42.08	22817900
452	2-Hydroxyisobutyrylation	AKVEDLLKRPDVDVN CCHHHHHHCCCCCCC	70.00	-
452	Ubiquitination	AKVEDLLKRPDVDVN CCHHHHHHCCCCCCC	70.00	32015554
459	Ubiquitination	KRPDVDVNGQCAGHT HCCCCCCCCCCCCHH	30.29	21890473
481	Ubiquitination	NGHVDILKLLLKQNV CCCHHHHHHHHHCCC	37.11	22817900
485	Neddylation	DILKLLLKQNVDVEA HHHHHHHHCCCCCCE	39.53	32015554
485	Ubiquitination	DILKLLLKQNVDVEA HHHHHHHHCCCCCCE	39.53	21906983
495	Ubiquitination	VDVEAEDKDGDRAVH CCCCEECCCCCCEEE	55.93	21906983
519	Ubiquitination	VIEVLHRGSADLNAR EEEEEECCCCCCCCC	19.21	22053931
540	Ubiquitination	PLHIAVNKGHLQVVK CEEEEEECCHHHHHH	40.90	21890473
547	Ubiquitination	KGHLQVVKTLLDFGC CCHHHHHHHHHHCCC	34.30	32015554
554	Ubiquitination	KTLLDFGCHPSLQDS HHHHHCCCCCHHCCC	4.27	21890473
563	Ubiquitination	PSLQDSEGDTPLHDA CHHCCCCCCCCHHHH	48.31	21890473
573	Ubiquitination	PLHDAISKKRDDILA CHHHHHHCCHHHHHH	45.79	32015554
577	Ubiquitination	AISKKRDDILAVLLE HHHCCHHHHHHHHHH	41.66	21890473
578	Ubiquitination	ISKKRDDILAVLLEA HHCCHHHHHHHHHHC	2.89	22817900
599	Ubiquitination	TNNNGFNALHHAALR ECCCCCHHHHHHHHC	13.15	22505724
600	Ubiquitination	NNNGFNALHHAALRG CCCCCHHHHHHHHCC	2.97	22505724
615	Ubiquitination	NPSAMRVLLSKLPRP CHHHHHHHHCCCCCC	2.93	21890473
618	Ubiquitination	AMRVLLSKLPRPWIV HHHHHHCCCCCCEEE	63.58	22817900
619	Ubiquitination	MRVLLSKLPRPWIVD HHHHHCCCCCCEEEC	3.80	22817900
622	Ubiquitination	LLSKLPRPWIVDEKK HHCCCCCCEEECCCC	24.17	22817900
624	Ubiquitination	SKLPRPWIVDEKKDD CCCCCCEEECCCCCC	2.87	22053931
654	Ubiquitination	VAELLVHQGNANLDI HHHHHHHCCCCCCEE	39.08	22817900
676	Ubiquitination	ALHLAVERQHTQIVR HHHHHHHHHHHHHHH	27.29	23503661
677	Ubiquitination	LHLAVERQHTQIVRL HHHHHHHHHHHHHHH	30.40	23503661
682	Ubiquitination	ERQHTQIVRLLVRAG HHHHHHHHHHHHHCC	2.25	23503661
691	Ubiquitination	LLVRAGAKLDIQDKD HHHHCCCCCCCCCCC	45.86	21906983
697	Ubiquitination	AKLDIQDKDGDTPLH CCCCCCCCCCCCCHH	47.43	29967540
700	Ubiquitination	DIQDKDGDTPLHEAL CCCCCCCCCCHHHHH	56.11	22817900
701	Ubiquitination	IQDKDGDTPLHEALR CCCCCCCCCHHHHHH	33.02	22817900
723	Ubiquitination	RQLQDMQDVGKVDAA HHHHHHHHHCCCCCC	44.49	21963094
724	Ubiquitination	QLQDMQDVGKVDAAW HHHHHHHHCCCCCCC	4.50	22817900
726	Neddylation	QDMQDVGKVDAAWEP HHHHHHCCCCCCCCC	36.47	32015554
726	Ubiquitination	QDMQDVGKVDAAWEP HHHHHHCCCCCCCCC	36.47	27667366
735	Neddylation	DAAWEPSKNTLIMGL CCCCCCCCCEEEEEC	65.74	32015554
735	Ubiquitination	DAAWEPSKNTLIMGL CCCCCCCCCEEEEEC	65.74	27667366
744	Phosphorylation	TLIMGLGTQGAEKKS EEEEECCCCCCCHHH	28.95	28857561
745	Ubiquitination	LIMGLGTQGAEKKSA EEEECCCCCCCHHHH	47.50	23503661
749	Ubiquitination	LGTQGAEKKSAASIA CCCCCCCHHHHHHHH	52.04	21906983
750	Ubiquitination	GTQGAEKKSAASIAC CCCCCCHHHHHHHHH	36.51	22817900
764	Ubiquitination	CFLAANGADLSIRNK HHHHHCCCCEEECCC	17.87	27667366
766	Ubiquitination	LAANGADLSIRNKKG HHHCCCCEEECCCCC	4.55	22817900
767	Phosphorylation	AANGADLSIRNKKGQ HHCCCCEEECCCCCC	21.76	24719451
771	Ubiquitination	ADLSIRNKKGQSPLD CCEEECCCCCCCCHH	48.72	22505724
772	Ubiquitination	DLSIRNKKGQSPLDL CEEECCCCCCCCHHC	66.77	33845483
777	Ubiquitination	NKKGQSPLDLCPDPN CCCCCCCHHCCCCHH	10.72	21890473
787	Neddylation	CPDPNLCKALAKCHK CCCHHHHHHHHHHHH	49.99	32015554
787	Ubiquitination	CPDPNLCKALAKCHK CCCHHHHHHHHHHHH	49.99	21906983
791	Ubiquitination	NLCKALAKCHKEKVS HHHHHHHHHHHHHCC	37.41	22817900
794	Ubiquitination	KALAKCHKEKVSGQV HHHHHHHHHHCCCCC	69.56	22817900
796	Ubiquitination	LAKCHKEKVSGQVGS HHHHHHHHCCCCCCC	46.64	21906983
798	Phosphorylation	KCHKEKVSGQVGSRS HHHHHHCCCCCCCCC	34.28	26074081
803	Phosphorylation	KVSGQVGSRSPSMIS HCCCCCCCCCHHHCC	30.63	30576142
805	Phosphorylation	SGQVGSRSPSMISND CCCCCCCCHHHCCCC	24.03	25159151
807	Phosphorylation	QVGSRSPSMISNDSE CCCCCCHHHCCCCCC	29.96	23663014
810	Phosphorylation	SRSPSMISNDSETLE CCCHHHCCCCCCCHH	26.41	23663014
813	Phosphorylation	PSMISNDSETLEECM HHHCCCCCCCHHHHC	36.41	23663014
815	Phosphorylation	MISNDSETLEECMVC HCCCCCCCHHHHCCC	43.34	23663014
826	Ubiquitination	CMVCSDMKRDTLFGP HCCCCCCCCCCCCCC	52.71	21906983
848	Ubiquitination	SLCSPRVKKCLICKE HHCCCCCCEEEECHH	38.76	23503661
849	Ubiquitination	LCSPRVKKCLICKEQ HCCCCCCEEEECHHH	31.27	23503661
854	Neddylation	VKKCLICKEQVQSRT CCEEEECHHHHCCCC	44.16	32015554
854	Ubiquitination	VKKCLICKEQVQSRT CCEEEECHHHHCCCC	44.16	32015554
859	Phosphorylation	ICKEQVQSRTKIEEC ECHHHHCCCCCCEEE	42.96	29083192
861	Phosphorylation	KEQVQSRTKIEECVV HHHHCCCCCCEEEEE	41.44	29083192
862	Ubiquitination	EQVQSRTKIEECVVC HHHCCCCCCEEEEEC	47.19	32015554
872	Acetylation	ECVVCSDKKAAVLFQ EEEECCCCCEEEEEE	28.19	27452117
872	Ubiquitination	ECVVCSDKKAAVLFQ EEEECCCCCEEEEEE	28.19	21906983
873	Ubiquitination	CVVCSDKKAAVLFQP EEECCCCCEEEEEEC	46.71	22817900
895	Neddylation	ENCANLMKKCVQCRA HHHHHHHHHHHHCHH	46.30	32015554
895	Ubiquitination	ENCANLMKKCVQCRA HHHHHHHHHHHHCHH	46.30	21963094
896	Ubiquitination	NCANLMKKCVQCRAV HHHHHHHHHHHCHHH	26.18	22817900
917	Ubiquitination	FIMCCGGKSSEDATD EEEECCCCCCCCCCC	35.77	23503661
927	Phosphorylation	EDATDDISSGNIPVL CCCCCCCCCCCCCEE	38.91	28555341
928	Phosphorylation	DATDDISSGNIPVLQ CCCCCCCCCCCCEEE	35.87	27251275
936	Ubiquitination	GNIPVLQKDKDNTNV CCCCEEEECCCCCCC	63.69	21906983
938	Ubiquitination	IPVLQKDKDNTNVNA CCEEEECCCCCCCCH	60.97	21906983
949	Neddylation	NVNADVQKLQQQLQD CCCHHHHHHHHHHHH	48.38	32015554
949	Ubiquitination	NVNADVQKLQQQLQD CCCHHHHHHHHHHHH	48.38	22817900
958	Ubiquitination	QQQLQDIKEQTMCPV HHHHHHHHHHHCCHH	52.65	32015554
990	Phosphorylation	QLCGDRMSECPICRK HHCCCHHHCCHHHHH	36.62	23898821

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
-	K	Ubiquitination	E3 ubiquitin ligase	MIB1	Q86YT6	PMID:12351649

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MIB1_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MIB1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RYK_HUMAN	RYK	physical	21875946
DLL1_HUMAN	DLL1	physical	21985982
TBK1_HUMAN	TBK1	physical	21903422
SNX5_HUMAN	SNX5	physical	16857196
DAPK1_HUMAN	DAPK1	physical	15048887
CMIP_HUMAN	CMIP	physical	20018188
UBE2N_HUMAN	UBE2N	physical	21903422
SMN_HUMAN	SMN1	physical	23615451
PCM1_HUMAN	PCM1	physical	24121310
MIB1_HUMAN	MIB1	physical	24239288
USP9X_HUMAN	USP9X	physical	24239288
ZC3H1_HUMAN	ZFC3H1	physical	24239288
RAGP1_HUMAN	RANGAP1	physical	24239288
BLM_HUMAN	BLM	physical	24239288
ZBT10_HUMAN	ZBTB10	physical	24239288
GEMI4_HUMAN	GEMIN4	physical	24239288
MCM3_HUMAN	MCM3	physical	24239288
TOP3A_HUMAN	TOP3A	physical	24239288
DDB1_HUMAN	DDB1	physical	24239288
TOP3B_HUMAN	TOP3B	physical	24239288
RBBP4_HUMAN	RBBP4	physical	24239288
MCM7_HUMAN	MCM7	physical	24239288
CDC27_HUMAN	CDC27	physical	24239288
UB2D3_HUMAN	UBE2D3	physical	24239288
PLK4_HUMAN	PLK4	physical	25795303
JAG1_HUMAN	JAG1	physical	25747658
DL_DROME	Dl	physical	25747658
UB2D2_HUMAN	UBE2D2	physical	25747658
E41LA_HUMAN	EPB41L4A	physical	27510968

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• 615092: Left ventricular non-compaction 7 (LVNC7)
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.