TOP3B_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: TOP3B_HUMAN
• UniProt AC: O95985
• Protein Name: DNA topoisomerase 3-beta-1
• Gene Name: TOP3B
• Organism: Homo sapiens (Human).
• Sequence Length: 862
• Protein Description: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity..
• Protein Sequence: MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
3	Phosphorylation	-----MKTVLMVAEK -----CCEEEEEECC	19.99	29083192
12	Phosphorylation	LMVAEKPSLAQSIAK EEEECCHHHHHHHHH	47.61	29083192
16	Phosphorylation	EKPSLAQSIAKILSR CCHHHHHHHHHHHHC	20.93	29083192
19	Ubiquitination	SLAQSIAKILSRGSL HHHHHHHHHHHCCCC	42.37	21890473
19 (in isoform 3)	Ubiquitination	-	42.37	21890473
19 (in isoform 1)	Ubiquitination	-	42.37	21890473
19 (in isoform 2)	Ubiquitination	-	42.37	21890473
70	Phosphorylation	TLDFLGKYNKWDKVD EHHHHCCCCCCCCCC	21.85	-
75	Ubiquitination	GKYNKWDKVDPAELF CCCCCCCCCCHHHHH	47.63	-
83	Phosphorylation	VDPAELFSQAPTEKK CCHHHHHHCCCCCCC	37.75	29083192
87	Phosphorylation	ELFSQAPTEKKEANP HHHHCCCCCCCCCCC	64.55	29083192
89	Ubiquitination	FSQAPTEKKEANPKL HHCCCCCCCCCCCCC	59.39	-
95	Acetylation	EKKEANPKLNMVKFL CCCCCCCCCCEEEEE	52.91	25953088
100 (in isoform 2)	Ubiquitination	-	29.59	21890473
100 (in isoform 1)	Ubiquitination	-	29.59	21890473
100	Acetylation	NPKLNMVKFLQVEGR CCCCCEEEEEEECCC	29.59	26051181
100 (in isoform 3)	Ubiquitination	-	29.59	21890473
100	Ubiquitination	NPKLNMVKFLQVEGR CCCCCEEEEEEECCC	29.59	21890473
139	Ubiquitination	AVLPVMNKAHGGEKT HHHHHHHHHCCCCCE	24.54	-
198	Ubiquitination	AFTRFQTKYFQGKYG CHHHHHHHHHCCCCC	33.03	-
236	Acetylation	HDKIQSFKPETYWVL CHHHHHCCCEEEEEE	47.38	26051181
254	Phosphorylation	VNTDKDRSLLLDWDR ECCCCCCCCEECHHH	33.24	29523821
281	Acetylation	LNMTKLEKEAQVEAT HHHHHHHHHHHHHHH	69.64	156179
281	Ubiquitination	LNMTKLEKEAQVEAT HHHHHHHHHHHHHHH	69.64	-
330	Phosphorylation	QTAERLYTQGYISYP HHHHHHHHCCCCCCC	21.80	28152594
333	Phosphorylation	ERLYTQGYISYPRTE HHHHHCCCCCCCCCC	4.11	23663014
335	Phosphorylation	LYTQGYISYPRTETT HHHCCCCCCCCCCCC	22.17	28152594
336	Phosphorylation	YTQGYISYPRTETTH HHCCCCCCCCCCCCC	6.28	28152594
351 (in isoform 2)	Ubiquitination	-	48.58	21890473
351 (in isoform 1)	Ubiquitination	-	48.58	21890473
351 (in isoform 3)	Ubiquitination	-	48.58	21890473
351	Ubiquitination	YPENFDLKGSLRQQA CCCCCCCCCHHHHHC	48.58	2190698
430	Phosphorylation	DCKYLQSTISFRIGP CCCHHCCEEEEEECC	14.00	23927012
432	Phosphorylation	KYLQSTISFRIGPEL CHHCCEEEEEECCCC	14.46	29496963
501	Phosphorylation	LTEAELITLMEKHGI CCHHHHHHHHHHCCC	32.45	-
526	Phosphorylation	NNICQRNYVTVESGR HCCCCCCCEEECCCC	10.33	29496907
566	Ubiquitination	TIRSAVEKQLNLIAQ HHHHHHHHHHHHHHH	54.19	-
590	Acetylation	GHTLDVFKRKFHYFV HHHHHHHHHHHHHHH	55.30	25953088
633	Phosphorylation	KCHRFMKYIQAKPSR HHHHHHHHHHCCCCC	6.10	28634120
637	Ubiquitination	FMKYIQAKPSRLHCS HHHHHHCCCCCEEEC	27.44	-
639	Phosphorylation	KYIQAKPSRLHCSHC HHHHCCCCCEEECCC	46.93	28634120
644	Phosphorylation	KPSRLHCSHCDETYT CCCCEEECCCCCEEE	19.19	28634120
657	Phosphorylation	YTLPQNGTIKLYKEL EECCCCCEEEEEEEE	23.61	28634120
662	Acetylation	NGTIKLYKELRCPLD CCEEEEEEEECCCCC	61.79	26051181
677	Phosphorylation	DFELVLWSSGSRGKS CCEEEEEECCCCCCC	22.21	27174698
678	Phosphorylation	FELVLWSSGSRGKSY CEEEEEECCCCCCCC	29.25	27174698
680	Phosphorylation	LVLWSSGSRGKSYPL EEEEECCCCCCCCCC	39.55	27174698
772	Phosphorylation	RVSADTCSVCEAALL EECCCCCHHHHHHHC	31.71	27251275
788	Phosphorylation	VDFNKAKSPLPGDET CCCCCCCCCCCCCCC	36.21	25159151
824	Methylation	ASCHPMHRGGPGRRQ HHCCCCCCCCCCCCC	45.58	115384017
829	Methylation	MHRGGPGRRQGRGRG CCCCCCCCCCCCCCC	30.77	115918741
853	Ubiquitination	NPRRPKDKMSALAAY CCCCCHHHHHHHHHH	40.66	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of TOP3B_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of TOP3B_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of TOP3B_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
RSMB_HUMAN	SNRPB	physical	11549288
CENPF_HUMAN	CENPF	physical	11549288
CHD8_HUMAN	CHD8	physical	11549288
CHIC2_HUMAN	CHIC2	physical	16189514
KR412_HUMAN	KRTAP4-12	physical	16189514
FBLN5_HUMAN	FBLN5	physical	11549288
GRN_HUMAN	GRN	physical	11549288
PNMA2_HUMAN	PNMA2	physical	25416956
TRIM1_HUMAN	MID2	physical	25416956
ATL4_HUMAN	ADAMTSL4	physical	25416956
TRI41_HUMAN	TRIM41	physical	25416956
ADIP_HUMAN	SSX2IP	physical	25416956
SPRE2_HUMAN	SPRED2	physical	25416956
TDRD3_HUMAN	TDRD3	physical	26344197
TDRD3_HUMAN	TDRD3	physical	28101374

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.