MANF_HUMAN - PTM Information in dbPTM

Basic Information of Protein

• UniProt ID: MANF_HUMAN
• UniProt AC: P55145
• Protein Name: Mesencephalic astrocyte-derived neurotrophic factor
• Gene Name: MANF
• Organism: Homo sapiens (Human).
• Sequence Length: 182
• Subcellular Localization: Secreted . Endoplasmic reticulum lumen . Sarcoplasmic reticulum lumen . Retained in the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER st
• Protein Description: Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. [PubMed: 12794311 Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra (By similarity Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death]
• Protein Sequence: MRRMWATQGLAVALALSVLPGSRALRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Overview of Protein Modification Sites with Functional and Structural Information

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MRRMWATQGLA ----CCCCHHHHHHH	1.69	15144186
7	Phosphorylation	-MRRMWATQGLAVAL -CCCCHHHHHHHHHH	13.99	14729942
14	Phosphorylation	TQGLAVALALSVLPG HHHHHHHHHHHCCCC	3.86	15144186
17	Phosphorylation	LAVALALSVLPGSRA HHHHHHHHCCCCCCC	18.92	14729942
20	Phosphorylation	ALALSVLPGSRALRP HHHHHCCCCCCCCCC	36.24	15144186
41	Phosphorylation	ISYLGRFYQDLKDRD HHHHHHHHHHHHHCC	10.35	28152594
45	2-Hydroxyisobutyrylation	GRFYQDLKDRDVTFS HHHHHHHHHCCCCCC	60.03	-
45	Acetylation	GRFYQDLKDRDVTFS HHHHHHHHHCCCCCC	60.03	27452117
45	Ubiquitination	GRFYQDLKDRDVTFS HHHHHHHHHCCCCCC	60.03	-
48	Ubiquitination	YQDLKDRDVTFSPAT HHHHHHCCCCCCCHH	54.67	-
50	Phosphorylation	DLKDRDVTFSPATIE HHHHCCCCCCCHHHH	23.76	21406692
52	Phosphorylation	KDRDVTFSPATIENE HHCCCCCCCHHHHHH	12.31	21406692
55	Phosphorylation	DVTFSPATIENELIK CCCCCCHHHHHHHHH	32.01	21406692
62	Acetylation	TIENELIKFCREARG HHHHHHHHHHHHHCC	51.54	26051181
65	Ubiquitination	NELIKFCREARGKEN HHHHHHHHHHCCCCC	43.68	-
75	Glutathionylation	RGKENRLCYYIGATD CCCCCEEEEEEECCC	1.88	22555962
76	Phosphorylation	GKENRLCYYIGATDD CCCCEEEEEEECCCH	11.60	28152594
77	Phosphorylation	KENRLCYYIGATDDA CCCEEEEEEECCCHH	7.59	28152594
79	Phosphorylation	NRLCYYIGATDDAAT CEEEEEEECCCHHHH	13.30	-
91	Ubiquitination	AATKIINEVSKPLAH HHHHHHHHHCHHHHH	37.45	21890473
93	Phosphorylation	TKIINEVSKPLAHHI HHHHHHHCHHHHHCC	23.52	24719451
94	Ubiquitination	KIINEVSKPLAHHIP HHHHHHCHHHHHCCC	49.58	2189047
94	Acetylation	KIINEVSKPLAHHIP HHHHHHCHHHHHCCC	49.58	23749302
97	Acetylation	NEVSKPLAHHIPVEK HHHCHHHHHCCCHHH	10.76	-
97	Ubiquitination	NEVSKPLAHHIPVEK HHHCHHHHHCCCHHH	10.76	-
104	Acetylation	AHHIPVEKICEKLKK HHCCCHHHHHHHHCC	53.54	25953088
114	Phosphorylation	EKLKKKDSQICELKY HHHCCCCCCCHHHHC	30.26	21712546
117	Phosphorylation	KKKDSQICELKYDKQ CCCCCCCHHHHCCCC	3.77	-
120	Acetylation	DSQICELKYDKQIDL CCCCHHHHCCCCCCC	29.16	25038526
121	Phosphorylation	SQICELKYDKQIDLS CCCHHHHCCCCCCCC	40.78	-
123	Acetylation	ICELKYDKQIDLSTV CHHHHCCCCCCCCCC	46.17	26051181
123	2-Hydroxyisobutyrylation	ICELKYDKQIDLSTV CHHHHCCCCCCCCCC	46.17	-
123	Ubiquitination	ICELKYDKQIDLSTV CHHHHCCCCCCCCCC	46.17	-
126	Ubiquitination	LKYDKQIDLSTVDLK HHCCCCCCCCCCCHH	31.88	-
128	Phosphorylation	YDKQIDLSTVDLKKL CCCCCCCCCCCHHHH	23.72	20068231
129	Phosphorylation	DKQIDLSTVDLKKLR CCCCCCCCCCHHHHC	25.90	20068231
131	Phosphorylation	QIDLSTVDLKKLRVK CCCCCCCCHHHHCHH	53.51	20068231
133	Acetylation	DLSTVDLKKLRVKEL CCCCCCHHHHCHHHH	45.35	27452117
133	2-Hydroxyisobutyrylation	DLSTVDLKKLRVKEL CCCCCCHHHHCHHHH	45.35	-
133	Succinylation	DLSTVDLKKLRVKEL CCCCCCHHHHCHHHH	45.35	23954790
155	Ubiquitination	GETCKGCAEKSDYIR HHHCCHHHHHHHHHH	35.60	-
157	Acetylation	TCKGCAEKSDYIRKI HCCHHHHHHHHHHHH	30.06	25953088
157	2-Hydroxyisobutyrylation	TCKGCAEKSDYIRKI HCCHHHHHHHHHHHH	30.06	-
168	Sulfoxidation	IRKINELMPKYAPKA HHHHHHHCHHHCCHH	1.88	21406390
177	Ubiquitination	KYAPKAASARTDL-- HHCCHHHHHCCCC--	24.12	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Oops, there are no upstream regulatory protein records of MANF_HUMAN !!

Functions of PTM Sites

Oops, there are no descriptions of PTM sites of MANF_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Oops, there are no SNP-PTM records of MANF_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CA123_HUMAN	C1orf123	physical	26344197
B2L15_HUMAN	BCL2L15	physical	28514442

Drug and Disease Associations

• Kegg Disease
• There are no disease associations of PTM sites.
• OMIM Disease
• There are no disease associations of PTM sites.
• Kegg Drug
• There are no disease associations of PTM sites.
• DrugBank
• There are no disease associations of PTM sites.