dbPTM

NDC80_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	NDC80_HUMAN
UniProt AC	O14777
Protein Name	Kinetochore protein NDC80 homolog
Gene Name	NDC80
Organism	Homo sapiens (Human).
Sequence Length	642
Subcellular Localization	Nucleus. Chromosome, centromere, kinetochore. Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Protein Description	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. [PubMed: 9315664]
Protein Sequence	MKRSSVSSGGAGRLSMQELRSQDVNKQGLYTPQTKEKPTFGKLSINKPTSERKVSLFGKRTSGHGSRNSQLGIFSSSEKIKDPRPLNDKAFIQQCIRQLCEFLTENGYAHNVSMKSLQAPSVKDFLKIFTFLYGFLCPSYELPDTKFEEEVPRIFKDLGYPFALSKSSMYTVGAPHTWPHIVAALVWLIDCIKIHTAMKESSPLFDDGQPWGEETEDGIMHNKLFLDYTIKCYESFMSGADSFDEMNAELQSKLKDLFNVDAFKLESLEAKNRALNEQIARLEQEREKEPNRLESLRKLKASLQGDVQKYQAYMSNLESHSAILDQKLNGLNEEIARVELECETIKQENTRLQNIIDNQKYSVADIERINHERNELQQTINKLTKDLEAEQQKLWNEELKYARGKEAIETQLAEYHKLARKLKLIPKGAENSKGYDFEIKFNPEAGANCLVKYRAQVYVPLKELLNETEEEINKALNKKMGLEDTLEQLNAMITESKRSVRTLKEEVQKLDDLYQQKIKEAEEEDEKCASELESLEKHKHLLESTVNQGLSEAMNELDAVQREYQLVVQTTTEERRKVGNNLQRLLEMVATHVGSVEKHLEEQIAKVDREYEECMSEDLSENIKEIRDKYEKKATLIKSSEE

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
4	Phosphorylation	----MKRSSVSSGGA ----CCCCCCCCCCC	29.58	25849741
5	Phosphorylation	---MKRSSVSSGGAG ---CCCCCCCCCCCC	30.01	25849741
7	Phosphorylation	-MKRSSVSSGGAGRL -CCCCCCCCCCCCCC	25.80	26699800
8	Phosphorylation	MKRSSVSSGGAGRLS CCCCCCCCCCCCCCC	38.81	26699800
15	Phosphorylation	SGGAGRLSMQELRSQ CCCCCCCCHHHHHCC	19.63	21815630
20	Dimethylation	RLSMQELRSQDVNKQ CCCHHHHHCCCCHHC	31.36	-
20	Methylation	RLSMQELRSQDVNKQ CCCHHHHHCCCCHHC	31.36	24377289
26	Ubiquitination	LRSQDVNKQGLYTPQ HHCCCCHHCCCCCCC	45.66	21890473
30	Phosphorylation	DVNKQGLYTPQTKEK CCHHCCCCCCCCCCC	24.17	25159151
31	Phosphorylation	VNKQGLYTPQTKEKP CHHCCCCCCCCCCCC	17.60	22199227
34	Phosphorylation	QGLYTPQTKEKPTFG CCCCCCCCCCCCCCC	42.23	22199227
35	Ubiquitination	GLYTPQTKEKPTFGK CCCCCCCCCCCCCCC	59.50	-
37	Ubiquitination	YTPQTKEKPTFGKLS CCCCCCCCCCCCCCC	51.41	-
39	Phosphorylation	PQTKEKPTFGKLSIN CCCCCCCCCCCCCCC	56.97	20860994
42	Ubiquitination	KEKPTFGKLSINKPT CCCCCCCCCCCCCCC	34.57	-
44	Phosphorylation	KPTFGKLSINKPTSE CCCCCCCCCCCCCCC	27.83	17129782
47	Ubiquitination	FGKLSINKPTSERKV CCCCCCCCCCCCCEE	48.26	-
49	Phosphorylation	KLSINKPTSERKVSL CCCCCCCCCCCEEEE	44.84	25159151
50	Phosphorylation	LSINKPTSERKVSLF CCCCCCCCCCEEEEC	43.88	25159151
53	Acetylation	NKPTSERKVSLFGKR CCCCCCCEEEECCCC	31.93	7976623
55	Phosphorylation	PTSERKVSLFGKRTS CCCCCEEEECCCCCC	22.48	22617229
59	Ubiquitination	RKVSLFGKRTSGHGS CEEEECCCCCCCCCC	45.21	-
59	Methylation	RKVSLFGKRTSGHGS CEEEECCCCCCCCCC	45.21	7976633
59	Acetylation	RKVSLFGKRTSGHGS CEEEECCCCCCCCCC	45.21	7976633
61	Phosphorylation	VSLFGKRTSGHGSRN EEECCCCCCCCCCCC	42.41	25159151
62	Phosphorylation	SLFGKRTSGHGSRNS EECCCCCCCCCCCCC	32.59	25159151
66	Phosphorylation	KRTSGHGSRNSQLGI CCCCCCCCCCCCCCC	23.71	25159151
69	Phosphorylation	SGHGSRNSQLGIFSS CCCCCCCCCCCCCCC	26.18	19664994
75	Phosphorylation	NSQLGIFSSSEKIKD CCCCCCCCCCCCCCC	30.91	30266825
76	Phosphorylation	SQLGIFSSSEKIKDP CCCCCCCCCCCCCCC	31.59	23401153
77	Phosphorylation	QLGIFSSSEKIKDPR CCCCCCCCCCCCCCC	41.53	30266825
81	Ubiquitination	FSSSEKIKDPRPLND CCCCCCCCCCCCCCH	73.14	-
89	Ubiquitination	DPRPLNDKAFIQQCI CCCCCCHHHHHHHHH	44.09	-
123	Ubiquitination	SLQAPSVKDFLKIFT CCCCCCHHHHHHHHH	47.29	21890473
127	Ubiquitination	PSVKDFLKIFTFLYG CCHHHHHHHHHHHHH	35.45	-
153	Methylation	KFEEEVPRIFKDLGY CHHHHHHHHHHHHCC	52.75	115478397
156	Ubiquitination	EEVPRIFKDLGYPFA HHHHHHHHHHCCCEE	50.07	-
165	Phosphorylation	LGYPFALSKSSMYTV HCCCEEECCCCCCCC	27.36	18297113
201	Phosphorylation	IHTAMKESSPLFDDG HHHHHHHCCCCCCCC	31.56	25159151
202	Phosphorylation	HTAMKESSPLFDDGQ HHHHHHCCCCCCCCC	27.06	29523821
242	Phosphorylation	SFMSGADSFDEMNAE HHHCCCCCHHHHHHH	33.78	-
253	Ubiquitination	MNAELQSKLKDLFNV HHHHHHHHHHHHHCC	46.74	-
255	Ubiquitination	AELQSKLKDLFNVDA HHHHHHHHHHHCCCH	57.00	-
264	Ubiquitination	LFNVDAFKLESLEAK HHCCCHHHHHHHHHH	53.73	21890473
271	Ubiquitination	KLESLEAKNRALNEQ HHHHHHHHHHHHHHH	37.28	-
300	Ubiquitination	LESLRKLKASLQGDV HHHHHHHHHHHHHCH	38.68	-
309	Ubiquitination	SLQGDVQKYQAYMSN HHHHCHHHHHHHHHC	38.90	-
327	Ubiquitination	HSAILDQKLNGLNEE CHHHHHHHHCCCCHH	43.52	21890473
344	Phosphorylation	RVELECETIKQENTR HHHHHHHHHHCCCHH	45.17	30108239
346	Ubiquitination	ELECETIKQENTRLQ HHHHHHHHCCCHHHH	60.00	-
350	Phosphorylation	ETIKQENTRLQNIID HHHHCCCHHHHHHHC	31.81	30108239
360	Ubiquitination	QNIIDNQKYSVADIE HHHHCCCCCCHHHHH	45.83	21890473
361	Phosphorylation	NIIDNQKYSVADIER HHHCCCCCCHHHHHH	9.90	-
382	Ubiquitination	ELQQTINKLTKDLEA HHHHHHHHHHHHHHH	54.40	-
393	Ubiquitination	DLEAEQQKLWNEELK HHHHHHHHHHHHHHH	55.98	-
400	Ubiquitination	KLWNEELKYARGKEA HHHHHHHHHHHHHHH	39.89	-
405	Ubiquitination	ELKYARGKEAIETQL HHHHHHHHHHHHHHH	38.12	21890473
410	Phosphorylation	RGKEAIETQLAEYHK HHHHHHHHHHHHHHH	23.83	21406692
415	Phosphorylation	IETQLAEYHKLARKL HHHHHHHHHHHHHHC	9.92	21406692
417	Ubiquitination	TQLAEYHKLARKLKL HHHHHHHHHHHHCCC	42.83	-
433	Ubiquitination	PKGAENSKGYDFEIK CCCCCCCCCCCEEEE	73.42	-
452	Ubiquitination	AGANCLVKYRAQVYV CCCCCEEEEEEEEEE	18.97	-
458	Phosphorylation	VKYRAQVYVPLKELL EEEEEEEEEEHHHHH	5.58	22817900
462	Ubiquitination	AQVYVPLKELLNETE EEEEEEHHHHHHHCH	40.00	21890473
474	Ubiquitination	ETEEEINKALNKKMG HCHHHHHHHHHHHCC	60.97	-
485	Phosphorylation	KKMGLEDTLEQLNAM HHCCHHHHHHHHHHH	23.87	29759185
494	Phosphorylation	EQLNAMITESKRSVR HHHHHHHHHHHHHHH	22.56	20860994
496	Phosphorylation	LNAMITESKRSVRTL HHHHHHHHHHHHHHH	25.39	29759185
499	Phosphorylation	MITESKRSVRTLKEE HHHHHHHHHHHHHHH	21.49	20860994
502	Phosphorylation	ESKRSVRTLKEEVQK HHHHHHHHHHHHHHH	39.77	20860994
504	Ubiquitination	KRSVRTLKEEVQKLD HHHHHHHHHHHHHHH	51.74	-
509	Ubiquitination	TLKEEVQKLDDLYQQ HHHHHHHHHHHHHHH	60.09	-
517	Ubiquitination	LDDLYQQKIKEAEEE HHHHHHHHHHHHHHH	39.97	-
517	Acetylation	LDDLYQQKIKEAEEE HHHHHHHHHHHHHHH	39.97	23236377
527	Acetylation	EAEEEDEKCASELES HHHHHHHHHHHHHHH	47.63	20167786
530	Phosphorylation	EEDEKCASELESLEK HHHHHHHHHHHHHHH	52.78	27251275
534	Phosphorylation	KCASELESLEKHKHL HHHHHHHHHHHHHHH	55.22	21815630
591	Phosphorylation	RLLEMVATHVGSVEK HHHHHHHHHHHHHHH	12.99	-
595	Phosphorylation	MVATHVGSVEKHLEE HHHHHHHHHHHHHHH	26.47	27251275
606	Ubiquitination	HLEEQIAKVDREYEE HHHHHHHHHHHHHHH	46.42	-
611	Phosphorylation	IAKVDREYEECMSED HHHHHHHHHHHHCCC	20.23	27642862
616	Phosphorylation	REYEECMSEDLSENI HHHHHHHCCCHHHHH	39.35	28348404
639	Phosphorylation	KKATLIKSSEE---- HHHHHHHCCCC----	35.67	24719451

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
5	S	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
15	S	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
44	S	Phosphorylation	Kinase	AURKA	O14965	GPS
44	S	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
49	T	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
55	S	Phosphorylation	Kinase	AURKA	O14965	GPS
55	S	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
69	S	Phosphorylation	Kinase	AURKA	O14965	GPS
69	S	Phosphorylation	Kinase	AURKB	Q96GD4	GPS
165	S	Phosphorylation	Kinase	NEK1	Q96PY6	PSP
165	S	Phosphorylation	Kinase	NEK2	P51955	Uniprot
-	K	Ubiquitination	E3 ubiquitin ligase	FZR1	Q9UM11	PMID:21199005

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of NDC80_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of NDC80_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
BL1S6_HUMAN	BLOC1S6	physical	16189514
KLH12_HUMAN	KLHL12	physical	16189514
WASC3_HUMAN	CCDC53	physical	16189514
USBP1_HUMAN	USHBP1	physical	16189514
AMOL2_HUMAN	AMOTL2	physical	16189514
CACO1_HUMAN	CALCOCO1	physical	16189514
SPC24_HUMAN	SPC24	physical	14699129
SPC25_HUMAN	SPC25	physical	14699129
NUF2_HUMAN	NUF2	physical	14699129
TM1L1_HUMAN	TOM1L1	physical	16169070
NEK2_HUMAN	NEK2	physical	12386167
KIN3_YEAST	KIN3	physical	12386167
NEK2_HUMAN	NEK2	physical	9295362
PRS7_HUMAN	PSMC2	physical	9295362
NDC80_HUMAN	NDC80	physical	20360068
SPC24_HUMAN	SPC24	physical	20360068
SPC25_HUMAN	SPC25	physical	20360068
NUF2_HUMAN	NUF2	physical	20360068
SPC25_HUMAN	SPC25	physical	22939629
NUF2_HUMAN	NUF2	physical	22939629
SPC24_HUMAN	SPC24	physical	22939629
NSL1_HUMAN	NSL1	physical	22939629
PLK1_HUMAN	PLK1	physical	22939629
SKA1_HUMAN	SKA1	physical	17093495
NUF2_HUMAN	NUF2	physical	14602875
NDC80_HUMAN	NDC80	physical	14602875
AURKB_HUMAN	AURKB	physical	14602875
CENPH_HUMAN	CENPH	physical	15713649
NDC80_HUMAN	NDC80	physical	25416956
TFP11_HUMAN	TFIP11	physical	25416956
MED4_HUMAN	MED4	physical	25416956
WASC3_HUMAN	CCDC53	physical	25416956
CCHCR_HUMAN	CCHCR1	physical	25416956
CACO1_HUMAN	CALCOCO1	physical	25416956
NDEL1_HUMAN	NDEL1	physical	25416956
IFT20_HUMAN	IFT20	physical	25416956
SGF29_HUMAN	CCDC101	physical	25416956
HAUS1_HUMAN	HAUS1	physical	25416956
SFR1_HUMAN	SFR1	physical	25416956
KLC3_HUMAN	KLC3	physical	25416956
TXLNA_HUMAN	TXLNA	physical	25416956
RUFY4_HUMAN	RUFY4	physical	25416956
PLSI_HUMAN	PLS1	physical	26344197

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of NDC80_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Evaluation of the low-specificity protease elastase for large-scalephosphoproteome analysis."; Wang B., Malik R., Nigg E.A., Korner R.; Anal. Chem. 80:9526-9533(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-31; THR-34; SER-44;SER-55 AND SER-69, AND MASS SPECTROMETRY.	19007248 [Abstract]
"Phosphoproteome analysis of the human mitotic spindle."; Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.; Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-55; SER-62; SER-76 ANDSER-77, AND MASS SPECTROMETRY.	16565220 [Abstract]
"Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinaseis essential for faithful chromosome segregation."; Chen Y., Riley D.J., Zheng L., Chen P.-L., Lee W.-H.; J. Biol. Chem. 277:49408-49416(2002). Cited for: INTERACTION WITH NEK2, AND PHOSPHORYLATION AT SER-165 BY NEK2.	12386167 [Abstract]