dbPTM

SPC24_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	SPC24_HUMAN
UniProt AC	Q8NBT2
Protein Name	Kinetochore protein Spc24
Gene Name	SPC24
Organism	Homo sapiens (Human).
Sequence Length	197
Subcellular Localization	Nucleus . Chromosome, centromere, kinetochore . Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
Protein Description	Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. [PubMed: 14738735 Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore]
Protein Sequence	MAAFRDIEEVSQGLLSLLGANRAEAQQRRLLGRHEQVVERLLETQDGAEKQLREILTMEKEVAQSLLNAKEQVHQGGVELQQLEAGLQEAGEEDTRLKASLLYLTRELEELKEIEADLERQEKEVDEDTTVTIPSAVYVAQLYHQVSKIEWDYECEPGMVKGIHHGPSVAQPIHLDSTQLSRKFISDYLWSLVDTEW

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
11	Phosphorylation	FRDIEEVSQGLLSLL CCCHHHHHHHHHHHH	23.08	20873877
16	Phosphorylation	EVSQGLLSLLGANRA HHHHHHHHHHCCCHH	26.96	20873877
50	Acetylation	ETQDGAEKQLREILT HCCCHHHHHHHHHHH	54.84	23236377
50	Ubiquitination	ETQDGAEKQLREILT HCCCHHHHHHHHHHH	54.84	21890473
50	Ubiquitination	ETQDGAEKQLREILT HCCCHHHHHHHHHHH	54.84	21890473
50	Ubiquitination	ETQDGAEKQLREILT HCCCHHHHHHHHHHH	54.84	21890473
60	Ubiquitination	REILTMEKEVAQSLL HHHHHHHHHHHHHHH	46.82	-
65	Phosphorylation	MEKEVAQSLLNAKEQ HHHHHHHHHHHHHHH	25.70	28555341
70	Ubiquitination	AQSLLNAKEQVHQGG HHHHHHHHHHHHHCC	47.83	21890473
98	Ubiquitination	GEEDTRLKASLLYLT CCCCHHHHHHHHHHH	32.41	-
105	Phosphorylation	KASLLYLTRELEELK HHHHHHHHHHHHHHH	14.45	20068231
112	Ubiquitination	TRELEELKEIEADLE HHHHHHHHHHHHHHH	60.98	2190698
123	Ubiquitination	ADLERQEKEVDEDTT HHHHHHHHCCCCCCC	55.49	-
129	Phosphorylation	EKEVDEDTTVTIPSA HHCCCCCCCCCCCHH	22.55	20860994
130	Phosphorylation	KEVDEDTTVTIPSAV HCCCCCCCCCCCHHH	28.15	25159151
138	Phosphorylation	VTIPSAVYVAQLYHQ CCCCHHHHHHHHHHH	6.98	-
143	Phosphorylation	AVYVAQLYHQVSKIE HHHHHHHHHHHHCCC	4.33	-
161	Ubiquitination	ECEPGMVKGIHHGPS CCCCCCCCEECCCCC	43.16	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
Oops, there are no upstream regulatory protein records of SPC24_HUMAN !!

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of SPC24_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of SPC24_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
SKA3_HUMAN	SKA3	physical	22371557
SPC25_HUMAN	SPC25	physical	22371557
A4_HUMAN	APP	physical	21832049
SPC25_HUMAN	SPC25	physical	22939629
ASPM_HUMAN	ASPM	physical	26186194
KNL1_HUMAN	CASC5	physical	26186194
NDC80_HUMAN	NDC80	physical	26186194
SPC25_HUMAN	SPC25	physical	26186194
SGF29_HUMAN	CCDC101	physical	26186194
PMF1_HUMAN	PMF1	physical	26186194
PLD2_HUMAN	PLD2	physical	26186194
MIS12_HUMAN	MIS12	physical	26186194
NUF2_HUMAN	NUF2	physical	26186194
DNLI3_HUMAN	LIG3	physical	26496610
NDC80_HUMAN	NDC80	physical	26496610
S27A2_HUMAN	SLC27A2	physical	26496610
ZWINT_HUMAN	ZWINT	physical	26496610
PMF1_HUMAN	PMF1	physical	26496610
NU188_HUMAN	NUP188	physical	26496610
NSL1_HUMAN	NSL1	physical	26496610
KNL1_HUMAN	CASC5	physical	26496610
SPC25_HUMAN	SPC25	physical	26496610
MIS12_HUMAN	MIS12	physical	26496610
DYHC2_HUMAN	DYNC2H1	physical	26496610
DSN1_HUMAN	DSN1	physical	26496610
TRI56_HUMAN	TRIM56	physical	26496610
NUF2_HUMAN	NUF2	physical	26496610
ASCC2_HUMAN	ASCC2	physical	26496610
SPC25_HUMAN	SPC25	physical	28514442
NDC80_HUMAN	NDC80	physical	28514442
NUF2_HUMAN	NUF2	physical	28514442
MIS12_HUMAN	MIS12	physical	28514442
KNL1_HUMAN	CASC5	physical	28514442
SGF29_HUMAN	CCDC101	physical	28514442
PLD2_HUMAN	PLD2	physical	28514442
ASPM_HUMAN	ASPM	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of SPC24_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11, AND MASSSPECTROMETRY.	19690332 [Abstract]
"Phosphoproteome analysis of the human mitotic spindle."; Nousiainen M., Sillje H.H.W., Sauer G., Nigg E.A., Koerner R.; Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-130, AND MASSSPECTROMETRY.	16565220 [Abstract]