dbPTM

ZWINT_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	ZWINT_HUMAN
UniProt AC	O95229
Protein Name	ZW10 interactor
Gene Name	ZWINT
Organism	Homo sapiens (Human).
Sequence Length	277
Subcellular Localization	Nucleus. Chromosome, centromere, kinetochore. Localizes to kinetochores from late prophase to anaphase.
Protein Description	Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase..
Protein Sequence	MEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVDSQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAIKIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVRERKTGTQQELDRVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKPQQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
46	Phosphorylation	LVEFVVDSQKKDKLL EEEHHHCCCCCCHHH	32.08	22067460
48	Ubiquitination	EFVVDSQKKDKLLCS EHHHCCCCCCHHHHH	67.88	21906983
49	Ubiquitination	FVVDSQKKDKLLCSQ HHHCCCCCCHHHHHH	53.08	22817900
51	Ubiquitination	VDSQKKDKLLCSQLQ HCCCCCCHHHHHHHH	52.84	21906983
74	Ubiquitination	LAQEDTAKGLDPLAS HHCCHHHCCCCCCCC	63.20	33845483
81	Phosphorylation	KGLDPLASEDTSRQK CCCCCCCCCCHHHHH	43.62	19691289
84	Phosphorylation	DPLASEDTSRQKAIA CCCCCCCHHHHHHHH	23.04	19691289
85	Phosphorylation	PLASEDTSRQKAIAA CCCCCCHHHHHHHHH	45.06	19691289
88	Ubiquitination	SEDTSRQKAIAAKEQ CCCHHHHHHHHHHHH	40.42	22817900
93	Ubiquitination	RQKAIAAKEQWKELK HHHHHHHHHHHHHHH	41.22	21906983
93	Ubiquitination	RQKAIAAKEQWKELK HHHHHHHHHHHHHHH	41.22	21890473
97	Ubiquitination	IAAKEQWKELKATYR HHHHHHHHHHHHHHH	53.52	22817900
100	Ubiquitination	KEQWKELKATYREHV HHHHHHHHHHHHHHH	39.51	33845483
111	Ubiquitination	REHVEAIKIGLTKAL HHHHHHHHHHHHHHH	37.46	23000965
111	Ubiquitination	REHVEAIKIGLTKAL HHHHHHHHHHHHHHH	37.46	21890473
116	Ubiquitination	AIKIGLTKALTQMEE HHHHHHHHHHHHHHH	46.58	21890473
116	Ubiquitination	AIKIGLTKALTQMEE HHHHHHHHHHHHHHH	46.58	23000965
127	Ubiquitination	QMEEAQRKRTQLREA HHHHHHHHHHHHHHH	47.95	29967540
141	Ubiquitination	AFEQLQAKKQMAMEK HHHHHHHHHHHHHHH	30.49	21906983
142	Ubiquitination	FEQLQAKKQMAMEKR HHHHHHHHHHHHHHH	48.61	22817900
148	Ubiquitination	KKQMAMEKRRAVQNQ HHHHHHHHHHHHHHH	33.81	29967540
162	Ubiquitination	QWQLQQEKHLQHLAE HHHHHHHHHHHHHHH	45.32	29967540
171	Phosphorylation	LQHLAEVSAEVRERK HHHHHHHCHHHHHHC	15.37	27251275
171 (in isoform 2)	Phosphorylation	-	15.37	29523821
178	Ubiquitination	SAEVRERKTGTQQEL CHHHHHHCCCCHHHH	46.70	24816145
191	Ubiquitination	ELDRVFQKLGNLKQQ HHHHHHHHHHHHHHH	47.07	27667366
196	Sumoylation	FQKLGNLKQQAEQER HHHHHHHHHHHHHHH	44.35	-
196	Sumoylation	FQKLGNLKQQAEQER HHHHHHHHHHHHHHH	44.35	-
196	Ubiquitination	FQKLGNLKQQAEQER HHHHHHHHHHHHHHH	44.35	21906983
205	Ubiquitination	QAEQERDKLQRYQTF HHHHHHHHHHHHHHH	53.38	-
209	Phosphorylation	ERDKLQRYQTFLQLL HHHHHHHHHHHHHHH	10.03	29496907
217	Ubiquitination	QTFLQLLYTLQGKLL HHHHHHHHHHHHCCC	17.62	29967540
250	Phosphorylation	PTRPQEQSTGDTMGR CCCCCCCCCCCCCCC	33.34	21775627
251	Phosphorylation	TRPQEQSTGDTMGRD CCCCCCCCCCCCCCC	38.48	21775627
262	Phosphorylation	MGRDPGVSFKAVGLQ CCCCCCCCCEEEECC	27.26	21815630
264	Ubiquitination	RDPGVSFKAVGLQPA CCCCCCCEEEECCCC	34.36	29967540

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
250	S	Phosphorylation	Kinase	AURB	Q96GD4	PSP
251	T	Phosphorylation	Kinase	AURB	Q96GD4	PSP
262	S	Phosphorylation	Kinase	AURB	Q96GD4	PSP

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
Oops, there are no descriptions of PTM sites of ZWINT_HUMAN !!

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of ZWINT_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
DSN1_HUMAN	DSN1	physical	15502821
TRI17_HUMAN	TRIM17	physical	22023800
NSL1_HUMAN	NSL1	physical	20360068
CNBP_HUMAN	CNBP	physical	20360068
DDX5_HUMAN	DDX5	physical	20360068
TIF1B_HUMAN	TRIM28	physical	20360068
MIS12_HUMAN	MIS12	physical	20360068
TRIM5_HUMAN	TRIM5	physical	20360068
CENPC_HUMAN	CENPC	physical	20360068
BUB1_HUMAN	BUB1	physical	20360068
NUF2_HUMAN	NUF2	physical	20360068
CYR61_HUMAN	CYR61	physical	20360068
LMNB1_HUMAN	LMNB1	physical	20360068
RCN1_HUMAN	RCN1	physical	20360068
ZWINT_HUMAN	ZWINT	physical	20360068
SNP29_HUMAN	SNAP29	physical	20360068
SPC25_HUMAN	SPC25	physical	20360068
KNL1_HUMAN	CASC5	physical	20360068
BUB1B_HUMAN	BUB1B	physical	20360068
NDC80_HUMAN	NDC80	physical	20360068
SPC24_HUMAN	SPC24	physical	20360068
SMTN_HUMAN	SMTN	physical	20360068
HNRPF_HUMAN	HNRNPF	physical	20360068
HNRH1_HUMAN	HNRNPH1	physical	20360068
TTBK2_HUMAN	TTBK2	physical	20360068
RRBP1_HUMAN	RRBP1	physical	20360068
DDX17_HUMAN	DDX17	physical	20360068
DSN1_HUMAN	DSN1	physical	20360068
PHAX_HUMAN	PHAX	physical	21900206
KNL1_HUMAN	CASC5	physical	17981135
KNL1_HUMAN	CASC5	physical	21199919
A4_HUMAN	APP	physical	21832049
MIS12_HUMAN	MIS12	physical	15824131
NDC80_HUMAN	NDC80	physical	15824131
SPC24_HUMAN	SPC24	physical	15824131
KNL1_HUMAN	CASC5	physical	15824131
DSN1_HUMAN	DSN1	physical	15824131
SMG5_HUMAN	SMG5	physical	15824131
ZW10_HUMAN	ZW10	physical	15824131
RRBP1_HUMAN	RRBP1	physical	26186194
KNL1_HUMAN	CASC5	physical	26186194
STX18_HUMAN	STX18	physical	26186194
DSN1_HUMAN	DSN1	physical	26186194
SPC24_HUMAN	SPC24	physical	26186194
NDC80_HUMAN	NDC80	physical	26186194
PMF1_HUMAN	PMF1	physical	26186194
LYRM4_HUMAN	LYRM4	physical	26186194
MTMR6_HUMAN	MTMR6	physical	26186194
NUF2_HUMAN	NUF2	physical	26186194
MIS12_HUMAN	MIS12	physical	26186194
NFS1_HUMAN	NFS1	physical	26186194
SPC25_HUMAN	SPC25	physical	26186194
FBX28_HUMAN	FBXO28	physical	26186194
BUB1_HUMAN	BUB1	physical	26496610
AP3S1_HUMAN	AP3S1	physical	26496610
CO1A2_HUMAN	COL1A2	physical	26496610
ROA1_HUMAN	HNRNPA1	physical	26496610
KIFC3_HUMAN	KIFC3	physical	26496610
SSRG_HUMAN	SSR3	physical	26496610
STX3_HUMAN	STX3	physical	26496610
TAF12_HUMAN	TAF12	physical	26496610
HTF4_HUMAN	TCF12	physical	26496610
LAP2A_HUMAN	TMPO	physical	26496610
LAP2B_HUMAN	TMPO	physical	26496610
IFT88_HUMAN	IFT88	physical	26496610
EI2BG_HUMAN	EIF2B3	physical	26496610
RIN1_HUMAN	RIN1	physical	26496610
RHG32_HUMAN	ARHGAP32	physical	26496610
MED24_HUMAN	MED24	physical	26496610
ARPC5_HUMAN	ARPC5	physical	26496610
NDC80_HUMAN	NDC80	physical	26496610
PMF1_HUMAN	PMF1	physical	26496610
SCMH1_HUMAN	SCMH1	physical	26496610
NSL1_HUMAN	NSL1	physical	26496610
IPYR2_HUMAN	PPA2	physical	26496610
ASTE1_HUMAN	ASTE1	physical	26496610
CC174_HUMAN	CCDC174	physical	26496610
M3K20_HUMAN	ZAK	physical	26496610
DGCR8_HUMAN	DGCR8	physical	26496610
RAIN_HUMAN	RASIP1	physical	26496610
RABL6_HUMAN	RABL6	physical	26496610
BI2L1_HUMAN	BAIAP2L1	physical	26496610
KNL1_HUMAN	CASC5	physical	26496610
SPC25_HUMAN	SPC25	physical	26496610
ZSWM5_HUMAN	ZSWIM5	physical	26496610
E41L5_HUMAN	EPB41L5	physical	26496610
SUGP1_HUMAN	SUGP1	physical	26496610
SQOR_HUMAN	SQRDL	physical	26496610
RBM25_HUMAN	RBM25	physical	26496610
TPC_HUMAN	SLC25A19	physical	26496610
MIS12_HUMAN	MIS12	physical	26496610
IPO4_HUMAN	IPO4	physical	26496610
DSN1_HUMAN	DSN1	physical	26496610
NUF2_HUMAN	NUF2	physical	26496610
T126A_HUMAN	TMEM126A	physical	26496610
TJAP1_HUMAN	TJAP1	physical	26496610
LENG8_HUMAN	LENG8	physical	26496610
TYW3_HUMAN	TYW3	physical	26496610
SPC24_HUMAN	SPC24	physical	26496610
PLBL2_HUMAN	PLBD2	physical	26496610
DSN1_HUMAN	DSN1	physical	28514442
SPC25_HUMAN	SPC25	physical	28514442
KNL1_HUMAN	CASC5	physical	28514442
MTMR6_HUMAN	MTMR6	physical	28514442
NDC80_HUMAN	NDC80	physical	28514442
SPC24_HUMAN	SPC24	physical	28514442
MIS12_HUMAN	MIS12	physical	28514442
LYRM4_HUMAN	LYRM4	physical	28514442
RRBP1_HUMAN	RRBP1	physical	28514442
NFS1_HUMAN	NFS1	physical	28514442
NUF2_HUMAN	NUF2	physical	28514442
STX18_HUMAN	STX18	physical	28514442
PP1G_HUMAN	PPP1CC	physical	28514442

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
There are no disease associations of PTM sites.
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of ZWINT_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Evaluation of the low-specificity protease elastase for large-scalephosphoproteome analysis."; Wang B., Malik R., Nigg E.A., Korner R.; Anal. Chem. 80:9526-9533(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-81, AND MASSSPECTROMETRY.	19007248 [Abstract]