dbPTM

WNK1_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	WNK1_HUMAN
UniProt AC	Q9H4A3
Protein Name	Serine/threonine-protein kinase WNK1 {ECO:0000305}
Gene Name	WNK1 {ECO:0000312\|HGNC:HGNC:14540}
Organism	Homo sapiens (Human).
Sequence Length	2382
Subcellular Localization	Cytoplasm .
Protein Description	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (By similarity).; Isoform 3: Dominant-negative regulator of the longer isoform 1. Does not have kinase activity, does not directly inhibit WNK4 and has no direct effect on sodium and chloride ion transport. Downregulates sodium-chloride cotransporter activity indirectly by inhibiting isoform 1, it associates with isoform 1 and attenuates its kinase activity. In kidney, may play an important role regulating sodium and potassium balance..
Protein Sequence	MSGGAAEKQSSTPGSLFLSPPAPAPKNGSSSDSSVGEKLGAAAADAVTGRTEEYRRRRHTMDKDSRGAAATTTTTEHRFFRRSVICDSNATALELPGLPLSLPQPSIPAAVPQSAPPEPHREETVTATATSQVAQQPPAAAAPGEQAVAGPAPSTVPSSTSKDRPVSQPSLVGSKEEPPPARSGSGGGSAKEPQEERSQQQDDIEELETKAVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNIVRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELAEEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKDRVSLIKRKREQRQLVREEQEKKKQEESSLKQQVEQSSASQTGIKQLPSASTGIPTASTTSASVSTQVEPEEPEADQHQQLQYQQPSISVLSDGTVDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTVPGHIPSTVQAQSQPHGVYPPSSVAQGQSQGQPSSSSLTGVSSSQPIQHPQQQQGIQQTAPPQQTVQYSLSQTSTSSEATTAQPVSQPQAPQVLPQVSAGKQLPVSQPVPTIQGEPQIPVATQPSVVPVHSGAHFLPVGQPLPTPLLPQYPVSQIPISTPHVSTAQTGFSSLPITMAAGITQPLLTLASSATTAAIPGVSTVVPSQLPTLLQPVTQLPSQVHPQLLQPAVQSMGIPANLGQAAEVPLSSGDVLYQGFPPRLPPQYPGDSNIAPSSNVASVCIHSTVLSPPMPTEVLATPGYFPTVVQPYVESNLLVPMGGVGGQVQVSQPGGSLAQAPTTSSQQAVLESTQGVSQVAPAEPVAVAQTQATQPTTLASSVDSAHSDVASGMSDGNENVPSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNPEEIATIMVNNDFILAIERESFVDQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFSGSQKLEGEFKQPIPASSMPQQIGIPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFPSEITDTVAASTAQSPGMNLSHSASSLSLQQAFSELRRAQMTEGPNTAPPNFSHTGPTFPVVPPFLSSIAGVPTTAAATAPVPATSSPPNDISTSVIQSEVTVPTEEGIAGVATSTGVVTSGGLPIPPVSESPVLSSVVSSITIPAVVSISTTSPSLQVPTSTSEIVVSSTALYPSVTVSATSASAGGSTATPGPKPPAVVSQQAAGSTTVGATLTSVSTTTSFPSTASQLCIQLSSSTSTPTLAETVVVSAHSLDKTSHSSTTGLAFSLSAPSSSSSPGAGVSSYISQPGGLHPLVIPSVIASTPILPQAAGPTSTPLLPQVPSIPPLVQPVANVPAVQQTLIHSQPQPALLPNQPHTHCPEVDSDTQPKAPGIDDIKTLEEKLRSLFSEHSSSGAQHASVSLETSLVIESTVTPGIPTTAVAPSKLLTSTTSTCLPPTNLPLGTVALPVTPVVTPGQVSTPVSTTTSGVKPGTAPSKPPLTKAPVLPVGTELPAGTLPSEQLPPFPGPSLTQSQQPLEDLDAQLRRTLSPEMITVTSAVGPVSMAAPTAITEAGTQPQKGVSQVKEGPVLATSSGAGVFKMGRFQVSVAADGAQKEGKNKSEDAKSVHFESSTSESSVLSSSSPESTLVKPEPNGITIPGISSDVPESAHKTTASEAKSDTGQPTKVGRFQVTTTANKVGRFSVSKTEDKITDTKKEGPVASPPFMDLEQAVLPAVIPKKEKPELSEPSHLNGPSSDPEAAFLSRDVDDGSGSPHSPHQLSSKSLPSQNLSQSLSNSFNSSYMSSDNESDIEDEDLKLELRRLRDKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSSRSSSLGNKSPQLSGNLSGQSAASVLHPQQTLHPPGNIPESGQNQLLQPLKPSPSSDNLYSAFTSDGAISVPSLSAPGQGTSSTNTVGATVNSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNYEGPGMARKFSAPGQLCISMTSNLGGSAPISAASATSLGHFTKSMCPPQQYGFPATPFGAQWSGTGGPAPQPLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2	O-linked_Glycosylation	------MSGGAAEKQ ------CCCCCCCCC	58.18	2507249
2	Phosphorylation	------MSGGAAEKQ ------CCCCCCCCC	58.18	24719451
10	Phosphorylation	GGAAEKQSSTPGSLF CCCCCCCCCCCCCEE	47.44	22199227
10	O-linked_Glycosylation	GGAAEKQSSTPGSLF CCCCCCCCCCCCCEE	47.44	30059200
11	Phosphorylation	GAAEKQSSTPGSLFL CCCCCCCCCCCCEEE	35.81	25159151
11	O-linked_Glycosylation	GAAEKQSSTPGSLFL CCCCCCCCCCCCEEE	35.81	30059200
12	Phosphorylation	AAEKQSSTPGSLFLS CCCCCCCCCCCEEEC	36.59	30266825
15	Phosphorylation	KQSSTPGSLFLSPPA CCCCCCCCEEECCCC	19.70	30266825
19	Phosphorylation	TPGSLFLSPPAPAPK CCCCEEECCCCCCCC	22.85	30266825
29	Phosphorylation	APAPKNGSSSDSSVG CCCCCCCCCCCCHHH	35.74	25247763
30	Phosphorylation	PAPKNGSSSDSSVGE CCCCCCCCCCCHHHH	39.29	24719451
31	Phosphorylation	APKNGSSSDSSVGEK CCCCCCCCCCHHHHH	43.12	27251789
33	Phosphorylation	KNGSSSDSSVGEKLG CCCCCCCCHHHHHHH	28.95	30624053
34	Phosphorylation	NGSSSDSSVGEKLGA CCCCCCCHHHHHHHH	38.85	25247763
48	Phosphorylation	AAAADAVTGRTEEYR HHHHHHHCCCCHHHH	23.75	23882029
51	Phosphorylation	ADAVTGRTEEYRRRR HHHHCCCCHHHHHHH	34.22	23882029
60	Phosphorylation	EYRRRRHTMDKDSRG HHHHHHHCCCCCCCC	25.83	22119528
65	Phosphorylation	RHTMDKDSRGAAATT HHCCCCCCCCCCCCC	38.05	23532336
66	Methylation	HTMDKDSRGAAATTT HCCCCCCCCCCCCCC	49.23	115920081
71	Phosphorylation	DSRGAAATTTTTEHR CCCCCCCCCCCCCCC	21.68	30576142
72	Phosphorylation	SRGAAATTTTTEHRF CCCCCCCCCCCCCCC	19.88	20068231
73	O-linked_Glycosylation	RGAAATTTTTEHRFF CCCCCCCCCCCCCCC	27.63	30059200
73	Phosphorylation	RGAAATTTTTEHRFF CCCCCCCCCCCCCCC	27.63	30387612
74	Phosphorylation	GAAATTTTTEHRFFR CCCCCCCCCCCCCCC	28.26	20068231
75	Phosphorylation	AAATTTTTEHRFFRR CCCCCCCCCCCCCCC	27.92	20068231
167	Phosphorylation	TSKDRPVSQPSLVGS CCCCCCCCCCCCCCC	38.77	29255136
170	Phosphorylation	DRPVSQPSLVGSKEE CCCCCCCCCCCCCCC	28.22	30266825
174	Phosphorylation	SQPSLVGSKEEPPPA CCCCCCCCCCCCCCC	29.43	29255136
175	Acetylation	QPSLVGSKEEPPPAR CCCCCCCCCCCCCCC	61.23	26051181
183	Phosphorylation	EEPPPARSGSGGGSA CCCCCCCCCCCCCCC	39.14	26846344
183	O-linked_Glycosylation	EEPPPARSGSGGGSA CCCCCCCCCCCCCCC	39.14	30059200
185	Phosphorylation	PPPARSGSGGGSAKE CCCCCCCCCCCCCCC	35.20	23401153
189	Phosphorylation	RSGSGGGSAKEPQEE CCCCCCCCCCCCHHH	39.17	26846344
198	Phosphorylation	KEPQEERSQQQDDIE CCCHHHHHHCHHCHH	35.30	29970186
209	Phosphorylation	DDIEELETKAVGMSN HCHHHHHHHHHCCCC	36.61	22468782
214	Sulfoxidation	LETKAVGMSNDGRFL HHHHHHCCCCCCCEE	2.45	30846556
215	Phosphorylation	ETKAVGMSNDGRFLK HHHHHCCCCCCCEEE	26.32	22468782
231	Phosphorylation	DIEIGRGSFKTVYKG EEEECCCCEEEEECC	23.39	28348404
236	Phosphorylation	RGSFKTVYKGLDTET CCCEEEEECCCCCCC	12.45	-
301	Phosphorylation	KKCIVLVTELMTSGT CEEEEEEEECCCCCH	21.17	30387612
306	Phosphorylation	LVTELMTSGTLKTYL EEEECCCCCHHHHHH	18.80	24719451
308	Phosphorylation	TELMTSGTLKTYLKR EECCCCCHHHHHHHH	25.50	24719451
312	Phosphorylation	TSGTLKTYLKRFKVM CCCHHHHHHHHHCHH	14.51	30387612
373	Phosphorylation	IGDLGLATLKRASFA ECHHHHHHHHHHHHH	37.54	21406692
375	Ubiquitination	DLGLATLKRASFAKS HHHHHHHHHHHHHHH	41.38	-
378	Phosphorylation	LATLKRASFAKSVIG HHHHHHHHHHHHHHC	29.75	21712546
382	Phosphorylation	KRASFAKSVIGTPEF HHHHHHHHHHCCCCH	18.61	25159151
386	Phosphorylation	FAKSVIGTPEFMAPE HHHHHHCCCCHHCHH	14.44	20639409
395	Phosphorylation	EFMAPEMYEEKYDES CHHCHHHHHHHCCCC	21.19	20639409
436	Phosphorylation	AQIYRRVTSGVKPAS HHHHHHHHCCCCCCC	20.25	29460479
437	Phosphorylation	QIYRRVTSGVKPASF HHHHHHHCCCCCCCC	38.44	29460479
443	Phosphorylation	TSGVKPASFDKVAIP HCCCCCCCCCCCCCH	42.37	23898821
469	Phosphorylation	QNKDERYSIKDLLNH CCCCCCCCHHHHHHH	29.41	24719451
563	Phosphorylation	KAIKDRVSLIKRKRE HHHHHHHHHHHHHHH	25.85	24719451
587	Phosphorylation	EKKKQEESSLKQQVE HHHHHHHHHHHHHHH	39.97	29978859
588	Phosphorylation	KKKQEESSLKQQVEQ HHHHHHHHHHHHHHH	42.75	29978859
596	Phosphorylation	LKQQVEQSSASQTGI HHHHHHHHHHHHHCC	17.86	20639409
597	Phosphorylation	KQQVEQSSASQTGIK HHHHHHHHHHHHCCC	31.47	25159151
599	Phosphorylation	QVEQSSASQTGIKQL HHHHHHHHHHCCCCC	30.17	25159151
601	Phosphorylation	EQSSASQTGIKQLPS HHHHHHHHCCCCCCC	38.08	25159151
676	Phosphorylation	VSSQQTVSYGSQHEQ ECCCEEEECCCCCCC	27.45	24275569
679	Phosphorylation	QQTVSYGSQHEQAHS CEEEECCCCCCCCCC	21.82	24275569
745 (in isoform 6)	Phosphorylation	-	2.08	27732954
745 (in isoform 7)	Phosphorylation	-	2.08	27732954
749 (in isoform 6)	Phosphorylation	-	14.94	27732954
749 (in isoform 7)	Phosphorylation	-	14.94	27732954
762 (in isoform 6)	Phosphorylation	-	30.51	27732954
762 (in isoform 7)	Phosphorylation	-	30.51	27732954
763 (in isoform 6)	Phosphorylation	-	20.75	27732954
763 (in isoform 7)	Phosphorylation	-	20.75	27732954
764 (in isoform 6)	Phosphorylation	-	39.39	27732954
764 (in isoform 7)	Phosphorylation	-	39.39	27732954
780 (in isoform 5)	Phosphorylation	-	21.40	30631047
782 (in isoform 5)	Phosphorylation	-	9.07	30631047
830 (in isoform 5)	Phosphorylation	-	34.09	27732954
834 (in isoform 5)	Phosphorylation	-	29.93	27732954
847 (in isoform 5)	Phosphorylation	-	30.45	27732954
848 (in isoform 5)	Phosphorylation	-	20.38	27732954
849 (in isoform 5)	Phosphorylation	-	21.06	27732954
873 (in isoform 6)	Phosphorylation	-	4.03	22199227
873 (in isoform 7)	Phosphorylation	-	4.03	22199227
876 (in isoform 6)	Phosphorylation	-	4.37	22199227
876 (in isoform 7)	Phosphorylation	-	4.37	22199227
877 (in isoform 6)	Phosphorylation	-	9.01	22199227
877 (in isoform 7)	Phosphorylation	-	9.01	22199227
918 (in isoform 6)	Phosphorylation	-	10.06	25850435
918 (in isoform 7)	Phosphorylation	-	10.06	25850435
924 (in isoform 6)	Phosphorylation	-	1.87	25850435
924 (in isoform 7)	Phosphorylation	-	1.87	25850435
927 (in isoform 6)	Phosphorylation	-	39.07	25850435
927 (in isoform 7)	Phosphorylation	-	39.07	25850435
928 (in isoform 6)	Phosphorylation	-	6.13	25850435
928 (in isoform 7)	Phosphorylation	-	6.13	25850435
930 (in isoform 6)	Phosphorylation	-	40.33	25850435
930 (in isoform 7)	Phosphorylation	-	40.33	25850435
950 (in isoform 6)	Phosphorylation	-	10.02	25850435
950 (in isoform 7)	Phosphorylation	-	10.02	25850435
956 (in isoform 6)	Phosphorylation	-	46.15	27732954
956 (in isoform 7)	Phosphorylation	-	46.15	27732954
958 (in isoform 5)	Phosphorylation	-	35.73	22199227
961 (in isoform 5)	Phosphorylation	-	11.37	22199227
961 (in isoform 6)	Phosphorylation	-	11.37	27732954
961 (in isoform 7)	Phosphorylation	-	11.37	27732954
962 (in isoform 5)	Phosphorylation	-	34.20	22199227
1003 (in isoform 5)	Phosphorylation	-	7.69	25850435
1009 (in isoform 5)	Phosphorylation	-	14.51	25850435
1012 (in isoform 5)	Phosphorylation	-	23.29	25850435
1013 (in isoform 5)	Phosphorylation	-	19.28	25850435
1015 (in isoform 5)	Phosphorylation	-	22.66	25850435
1035 (in isoform 5)	Phosphorylation	-	5.46	25850435
1041 (in isoform 5)	Phosphorylation	-	22.57	27732954
1046 (in isoform 5)	Phosphorylation	-	7.02	27732954
1098	Phosphorylation	GRHEGRTTKRHYRKS CCCCCCCCHHHHHHH	25.39	24719451
1102	Phosphorylation	GRTTKRHYRKSVRSR CCCCHHHHHHHHHHH	24.62	24719451
1104	Acetylation	TTKRHYRKSVRSRSR CCHHHHHHHHHHHCC	46.25	69523
1105	Phosphorylation	TKRHYRKSVRSRSRH CHHHHHHHHHHHCCC	17.48	-
1108	Phosphorylation	HYRKSVRSRSRHEKT HHHHHHHHHCCCCCC	32.24	-
1110	Phosphorylation	RKSVRSRSRHEKTSR HHHHHHHCCCCCCCC	38.96	-
1115	Phosphorylation	SRSRHEKTSRPKLRI HHCCCCCCCCCCEEE	27.30	-
1116	Phosphorylation	RSRHEKTSRPKLRIL HCCCCCCCCCCEEEE	58.62	-
1119	Acetylation	HEKTSRPKLRILNVS CCCCCCCCEEEEECC	49.65	69527
1128	Acetylation	RILNVSNKGDRVVEC EEEECCCCCCEEEEE	55.47	69531
1193	Phosphorylation	EKADEMLSEDVSVEP HHHHHHHCCCCCCCC	30.36	29978859
1197	Phosphorylation	EMLSEDVSVEPEGDQ HHHCCCCCCCCCCHH	32.84	29978859
1208	Phosphorylation	EGDQGLESLQGKDDY CCHHCHHHHCCCCCC	30.91	29978859
1215	Phosphorylation	SLQGKDDYGFSGSQK HHCCCCCCCCCCCCC	30.15	25159151
1218	Phosphorylation	GKDDYGFSGSQKLEG CCCCCCCCCCCCCEE	32.92	28674419
1220	Phosphorylation	DDYGFSGSQKLEGEF CCCCCCCCCCCEECC	23.97	29978859
1234	O-linked_Glycosylation	FKQPIPASSMPQQIG CCCCCCCCCCCCCCC	23.09	30059200
1234	Phosphorylation	FKQPIPASSMPQQIG CCCCCCCCCCCCCCC	23.09	24043423
1235	O-linked_Glycosylation	KQPIPASSMPQQIGI CCCCCCCCCCCCCCC	36.27	30059200
1235	Phosphorylation	KQPIPASSMPQQIGI CCCCCCCCCCCCCCC	36.27	24043423
1244	O-linked_Glycosylation	PQQIGIPTSSLTQVV CCCCCCCHHHHHHHH	29.09	30059200
1244	Phosphorylation	PQQIGIPTSSLTQVV CCCCCCCHHHHHHHH	29.09	24043423
1245	O-linked_Glycosylation	QQIGIPTSSLTQVVH CCCCCCHHHHHHHHH	20.28	30059200
1245	Phosphorylation	QQIGIPTSSLTQVVH CCCCCCHHHHHHHHH	20.28	24043423
1246	O-linked_Glycosylation	QIGIPTSSLTQVVHS CCCCCHHHHHHHHHH	37.60	30059200
1246	Phosphorylation	QIGIPTSSLTQVVHS CCCCCHHHHHHHHHH	37.60	24043423
1248	Phosphorylation	GIPTSSLTQVVHSAG CCCHHHHHHHHHHCC	22.67	24043423
1253	Phosphorylation	SLTQVVHSAGRRFIV HHHHHHHHCCCEEEE	22.32	24043423
1253	O-linked_Glycosylation	SLTQVVHSAGRRFIV HHHHHHHHCCCEEEE	22.32	30059200
1261	Phosphorylation	AGRRFIVSPVPESRL CCCEEEEECCCHHHH	18.50	19664994
1261	O-linked_Glycosylation	AGRRFIVSPVPESRL CCCEEEEECCCHHHH	18.50	30059200
1266	Phosphorylation	IVSPVPESRLRESKV EEECCCHHHHCCCCC	30.46	23403867
1276	O-linked_Glycosylation	RESKVFPSEITDTVA CCCCCCCHHHHHHHH	30.11	30059200
1276	Phosphorylation	RESKVFPSEITDTVA CCCCCCCHHHHHHHH	30.11	23663014
1279	O-linked_Glycosylation	KVFPSEITDTVAAST CCCCHHHHHHHHHHC	22.90	30059200
1279	Phosphorylation	KVFPSEITDTVAAST CCCCHHHHHHHHHHC	22.90	23663014
1281	Phosphorylation	FPSEITDTVAASTAQ CCHHHHHHHHHHCCC	11.91	23663014
1285	Phosphorylation	ITDTVAASTAQSPGM HHHHHHHHCCCCCCC	17.97	23663014
1285	O-linked_Glycosylation	ITDTVAASTAQSPGM HHHHHHHHCCCCCCC	17.97	30059200
1286	Phosphorylation	TDTVAASTAQSPGMN HHHHHHHCCCCCCCC	24.80	23663014
1289	O-linked_Glycosylation	VAASTAQSPGMNLSH HHHHCCCCCCCCCCC	22.43	30059200
1289	Phosphorylation	VAASTAQSPGMNLSH HHHHCCCCCCCCCCC	22.43	23663014
1295	Phosphorylation	QSPGMNLSHSASSLS CCCCCCCCCCHHHHH	15.47	28176443
1297	Phosphorylation	PGMNLSHSASSLSLQ CCCCCCCCHHHHHHH	26.86	28176443
1299	O-linked_Glycosylation	MNLSHSASSLSLQQA CCCCCCHHHHHHHHH	34.52	30059200
1299	Phosphorylation	MNLSHSASSLSLQQA CCCCCCHHHHHHHHH	34.52	23663014
1300	Phosphorylation	NLSHSASSLSLQQAF CCCCCHHHHHHHHHH	22.99	28176443
1302	Phosphorylation	SHSASSLSLQQAFSE CCCHHHHHHHHHHHH	26.53	23663014
1308	Phosphorylation	LSLQQAFSELRRAQM HHHHHHHHHHHHHHH	38.44	23663014
1521	O-linked_Glycosylation	STPTLAETVVVSAHS CCCCCEEEEEEEEEC	17.00	30059200
1528	Phosphorylation	TVVVSAHSLDKTSHS EEEEEEECCCCCCCC	37.67	24275569
1543	O-linked_Glycosylation	STTGLAFSLSAPSSS CCCEEEEEEECCCCC	18.79	30059200
1558	O-linked_Glycosylation	SSPGAGVSSYISQPG CCCCCCHHHHCCCCC	19.26	30059200
1559	O-linked_Glycosylation	SPGAGVSSYISQPGG CCCCCHHHHCCCCCC	24.87	30059200
1562	O-linked_Glycosylation	AGVSSYISQPGGLHP CCHHHHCCCCCCCCC	23.30	30059200
1653	Ubiquitination	APGIDDIKTLEEKLR CCCCCHHHHHHHHHH	54.79	-
1658	Ubiquitination	DIKTLEEKLRSLFSE HHHHHHHHHHHHHHH	39.56	-
1681	O-linked_Glycosylation	ASVSLETSLVIESTV EEEEEEEEEEEEEEC	15.97	30059200
1686	O-linked_Glycosylation	ETSLVIESTVTPGIP EEEEEEEEECCCCCC	19.63	OGP
1687	O-linked_Glycosylation	TSLVIESTVTPGIPT EEEEEEEECCCCCCC	18.66	30059200
1695	O-linked_Glycosylation	VTPGIPTTAVAPSKL CCCCCCCCCCCCHHH	17.49	30059200
1757	O-linked_Glycosylation	APSKPPLTKAPVLPV CCCCCCCCCCCCCCC	30.82	30059200
1803	Phosphorylation	LDAQLRRTLSPEMIT HHHHHHHHCCHHHEE	25.75	28348404
1805	Phosphorylation	AQLRRTLSPEMITVT HHHHHHCCHHHEEEE	20.43	24719451
1810	Phosphorylation	TLSPEMITVTSAVGP HCCHHHEEEEECCCC	19.20	18669648
1819	Phosphorylation	TSAVGPVSMAAPTAI EECCCCCCCCCCCCH	13.06	-
1827	O-linked_Glycosylation	MAAPTAITEAGTQPQ CCCCCCHHCCCCCCC	19.81	30059200
1838	Phosphorylation	TQPQKGVSQVKEGPV CCCCCCCCCCCCCCE	37.61	22468782
1848	Phosphorylation	KEGPVLATSSGAGVF CCCCEEEECCCCCEE	21.18	27794612
1848	O-linked_Glycosylation	KEGPVLATSSGAGVF CCCCEEEECCCCCEE	21.18	30059200
1849	Phosphorylation	EGPVLATSSGAGVFK CCCEEEECCCCCEEE	22.59	25159151
1849	O-linked_Glycosylation	EGPVLATSSGAGVFK CCCEEEECCCCCEEE	22.59	30059200
1850	Phosphorylation	GPVLATSSGAGVFKM CCEEEECCCCCEEEC	28.45	23403867
1850	O-linked_Glycosylation	GPVLATSSGAGVFKM CCEEEECCCCCEEEC	28.45	30059200
1863	O-linked_Glycosylation	KMGRFQVSVAADGAQ ECCEEEEEEECCCCC	8.68	30059200
1863	Phosphorylation	KMGRFQVSVAADGAQ ECCEEEEEEECCCCC	8.68	28857561
1874	Acetylation	DGAQKEGKNKSEDAK CCCCCCCCCCCCCCC	63.39	26051181
1876	Acetylation	AQKEGKNKSEDAKSV CCCCCCCCCCCCCCE	59.72	26051181
1882	Phosphorylation	NKSEDAKSVHFESST CCCCCCCCEEEECCC	23.65	26471730
1887	Phosphorylation	AKSVHFESSTSESSV CCCEEEECCCCCCCC	37.66	26471730
1888	Phosphorylation	KSVHFESSTSESSVL CCEEEECCCCCCCCC	28.84	26471730
1889	Phosphorylation	SVHFESSTSESSVLS CEEEECCCCCCCCCC	45.88	26471730
1890	Phosphorylation	VHFESSTSESSVLSS EEEECCCCCCCCCCC	37.11	26471730
1892	Phosphorylation	FESSTSESSVLSSSS EECCCCCCCCCCCCC	26.05	26471730
1893	Phosphorylation	ESSTSESSVLSSSSP ECCCCCCCCCCCCCC	24.03	26471730
1896	Phosphorylation	TSESSVLSSSSPEST CCCCCCCCCCCCCCC	26.47	26471730
1897	Phosphorylation	SESSVLSSSSPESTL CCCCCCCCCCCCCCC	30.58	26471730
1898	Phosphorylation	ESSVLSSSSPESTLV CCCCCCCCCCCCCCC	46.68	26471730
1899	Phosphorylation	SSVLSSSSPESTLVK CCCCCCCCCCCCCCC	34.33	26471730
1902	Phosphorylation	LSSSSPESTLVKPEP CCCCCCCCCCCCCCC	30.35	26471730
1903	Phosphorylation	SSSSPESTLVKPEPN CCCCCCCCCCCCCCC	33.63	26471730
1913	Phosphorylation	KPEPNGITIPGISSD CCCCCCEECCCCCCC	23.63	26471730
1928	Phosphorylation	VPESAHKTTASEAKS CCHHHCCCCHHHHCC	20.09	23312004
1929	O-linked_Glycosylation	PESAHKTTASEAKSD CHHHCCCCHHHHCCC	32.75	30059200
1929	Phosphorylation	PESAHKTTASEAKSD CHHHCCCCHHHHCCC	32.75	23312004
1931	O-linked_Glycosylation	SAHKTTASEAKSDTG HHCCCCHHHHCCCCC	35.67	30059200
1931	Phosphorylation	SAHKTTASEAKSDTG HHCCCCHHHHCCCCC	35.67	23403867
1935	Phosphorylation	TTASEAKSDTGQPTK CCHHHHCCCCCCCCE	47.67	23403867
1935	O-linked_Glycosylation	TTASEAKSDTGQPTK CCHHHHCCCCCCCCE	47.67	30059200
1937	Phosphorylation	ASEAKSDTGQPTKVG HHHHCCCCCCCCEEE	44.82	23403867
1941	Phosphorylation	KSDTGQPTKVGRFQV CCCCCCCCEEEEEEE	30.40	23286773
1949	O-linked_Glycosylation	KVGRFQVTTTANKVG EEEEEEEEECCCEEC	13.98	30059200
1950	O-linked_Glycosylation	VGRFQVTTTANKVGR EEEEEEEECCCEECC	25.67	30059200
1951	O-linked_Glycosylation	GRFQVTTTANKVGRF EEEEEEECCCEECCE	20.90	30059200
1954	Acetylation	QVTTTANKVGRFSVS EEEECCCEECCEEEE	43.53	25953088
1959	Phosphorylation	ANKVGRFSVSKTEDK CCEECCEEEECCCCC	24.91	22322096
1961	Phosphorylation	KVGRFSVSKTEDKIT EECCEEEECCCCCCC	32.44	25159151
1963	Phosphorylation	GRFSVSKTEDKITDT CCEEEECCCCCCCCC	41.74	24719451
1968	Phosphorylation	SKTEDKITDTKKEGP ECCCCCCCCCCCCCC	43.27	-
1970	Phosphorylation	TEDKITDTKKEGPVA CCCCCCCCCCCCCCC	35.05	-
1978	Phosphorylation	KKEGPVASPPFMDLE CCCCCCCCCCCCCHH	33.08	29255136
2002	Phosphorylation	KKEKPELSEPSHLNG CCCCCCCCCCCCCCC	45.88	23927012
2005	Phosphorylation	KPELSEPSHLNGPSS CCCCCCCCCCCCCCC	36.23	23927012
2011	Phosphorylation	PSHLNGPSSDPEAAF CCCCCCCCCCHHHHH	49.09	29255136
2012	Phosphorylation	SHLNGPSSDPEAAFL CCCCCCCCCHHHHHH	61.39	29255136
2020	Phosphorylation	DPEAAFLSRDVDDGS CHHHHHHCCCCCCCC	21.71	23927012
2027	Phosphorylation	SRDVDDGSGSPHSPH CCCCCCCCCCCCCHH	42.99	29255136
2029	Phosphorylation	DVDDGSGSPHSPHQL CCCCCCCCCCCHHHC	22.57	29255136
2032	Phosphorylation	DGSGSPHSPHQLSSK CCCCCCCCHHHCCCC	27.71	29255136
2037	Phosphorylation	PHSPHQLSSKSLPSQ CCCHHHCCCCCCCCC	29.20	22167270
2038	Phosphorylation	HSPHQLSSKSLPSQN CCHHHCCCCCCCCCC	34.55	22167270
2051	Phosphorylation	QNLSQSLSNSFNSSY CCHHHHHHHHHHHHH	35.36	27251275
2053	Phosphorylation	LSQSLSNSFNSSYMS HHHHHHHHHHHHHCC	23.56	27251275
2056	Phosphorylation	SLSNSFNSSYMSSDN HHHHHHHHHHCCCCC	22.34	27251275
2057	Phosphorylation	LSNSFNSSYMSSDNE HHHHHHHHHCCCCCC	26.42	27251275
2060	Phosphorylation	SFNSSYMSSDNESDI HHHHHHCCCCCCCCC	26.72	27251275
2061	Phosphorylation	FNSSYMSSDNESDIE HHHHHCCCCCCCCCC	28.11	29802988
2065	Phosphorylation	YMSSDNESDIEDEDL HCCCCCCCCCCCHHH	50.58	29802988
2102	Phosphorylation	KHEIESLYTKLGKVP HHHHHHHHHHHCCCC	15.88	25159151
2121	Phosphorylation	IPPAAPLSGRRRRPT ECCCCCCCCCCCCCC	29.24	26055452
2128	Phosphorylation	SGRRRRPTKSKGSKS CCCCCCCCCCCCCCC	46.25	23882029
2133	Phosphorylation	RPTKSKGSKSSRSSS CCCCCCCCCCCCCCC	32.85	22468782
2136	Phosphorylation	KSKGSKSSRSSSLGN CCCCCCCCCCCCCCC	39.66	28985074
2138	Phosphorylation	KGSKSSRSSSLGNKS CCCCCCCCCCCCCCC	26.65	24706070
2139	Phosphorylation	GSKSSRSSSLGNKSP CCCCCCCCCCCCCCC	28.59	26699800
2140	Phosphorylation	SKSSRSSSLGNKSPQ CCCCCCCCCCCCCCC	41.35	25849741
2145	Phosphorylation	SSSLGNKSPQLSGNL CCCCCCCCCCCCCCC	23.13	25159151
2188	Phosphorylation	LLQPLKPSPSSDNLY CCCCCCCCCCCCCCH	35.51	26074081
2190	Phosphorylation	QPLKPSPSSDNLYSA CCCCCCCCCCCCHHC	55.45	26074081
2191	Phosphorylation	PLKPSPSSDNLYSAF CCCCCCCCCCCHHCC	33.62	26074081
2195	Phosphorylation	SPSSDNLYSAFTSDG CCCCCCCHHCCCCCC	12.28	26074081
2196	Phosphorylation	PSSDNLYSAFTSDGA CCCCCCHHCCCCCCC	21.77	26074081
2243	Ubiquitination	PAMTSSRKGTFTDDL CCCCCCCCCCCCHHH	65.34	-
2245	Phosphorylation	MTSSRKGTFTDDLHK CCCCCCCCCCHHHHH	26.79	25159151
2247	Phosphorylation	SSRKGTFTDDLHKLV CCCCCCCCHHHHHHH	28.57	28450419
2265	Phosphorylation	ARDAMNLSGRRGSKG HHHHHHHCCCCCCCC	25.83	23401153
2270	Phosphorylation	NLSGRRGSKGHMNYE HHCCCCCCCCCCCCC	33.38	23401153
2276	Phosphorylation	GSKGHMNYEGPGMAR CCCCCCCCCCCCCCC	18.06	23927012
2286	Phosphorylation	PGMARKFSAPGQLCI CCCCCCCCCCCEEEE	36.27	28464451
2294	Phosphorylation	APGQLCISMTSNLGG CCCEEEEEECCCCCC	18.27	28450419
2296	Phosphorylation	GQLCISMTSNLGGSA CEEEEEECCCCCCCC	13.99	28450419
2297	Phosphorylation	QLCISMTSNLGGSAP EEEEEECCCCCCCCC	22.78	28450419
2302	O-linked_Glycosylation	MTSNLGGSAPISAAS ECCCCCCCCCCCHHH	29.13	30059200
2302	Phosphorylation	MTSNLGGSAPISAAS ECCCCCCCCCCCHHH	29.13	23090842
2306	O-linked_Glycosylation	LGGSAPISAASATSL CCCCCCCCHHHCHHH	19.41	30059200
2306	Phosphorylation	LGGSAPISAASATSL CCCCCCCCHHHCHHH	19.41	23090842
2309	Phosphorylation	SAPISAASATSLGHF CCCCCHHHCHHHCCC	31.62	23090842
2311	Phosphorylation	PISAASATSLGHFTK CCCHHHCHHHCCCCC	23.22	23090842
2312	Phosphorylation	ISAASATSLGHFTKS CCHHHCHHHCCCCCC	31.88	23090842
2317	Phosphorylation	ATSLGHFTKSMCPPQ CHHHCCCCCCCCCHH	19.28	23090842
2358	O-linked_Glycosylation	FQPVGTASLQNFNIS CCCCCCHHCCCCCCC	30.26	30059200
2370	Phosphorylation	NISNLQKSISNPPGS CCCCCHHHCCCCCCC	20.49	30266825
2370	O-linked_Glycosylation	NISNLQKSISNPPGS CCCCCHHHCCCCCCC	20.49	30059200
2372	Phosphorylation	SNLQKSISNPPGSNL CCCHHHCCCCCCCCC	50.83	26846344
2377	O-linked_Glycosylation	SISNPPGSNLRTT-- HCCCCCCCCCCCC--	38.16	30059200
2377	Phosphorylation	SISNPPGSNLRTT-- HCCCCCCCCCCCC--	38.16	23927012
2380	Methylation	NPPGSNLRTT----- CCCCCCCCCC-----	40.34	115388709
2381	O-linked_Glycosylation	PPGSNLRTT------ CCCCCCCCC------	39.77	30059200
2381	Phosphorylation	PPGSNLRTT------ CCCCCCCCC------	39.77	21712546
2382	O-linked_Glycosylation	PGSNLRTT------- CCCCCCCC-------	30.13	30059200
2382	Phosphorylation	PGSNLRTT------- CCCCCCCC-------	30.13	21712546

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
60	T	Phosphorylation	Kinase	AKT1	P31749	PSP
60	T	Phosphorylation	Kinase	RPS6KA5	O75582	GPS
60	T	Phosphorylation	Kinase	SGK1	O00141	PSP
60	T	Phosphorylation	Kinase	AKT-FAMILY	-	GPS
60	T	Phosphorylation	Kinase	PKB_GROUP	-	PhosphoELM
378	S	Phosphorylation	Kinase	WNK1	Q9H4A3	GPS
382	S	Phosphorylation	Kinase	WNK1	Q9H4A3	PSP
382	S	Phosphorylation	Kinase	WNK2	Q9Y3S1	PSP
382	S	Phosphorylation	Kinase	WNK3	Q9BYP7	PSP
2032	S	Phosphorylation	Kinase	MTOR	P42345	PSP
-	K	Ubiquitination	E3 ubiquitin ligase	KLHL3	Q9UH77	PMID:23387299
-	K	Ubiquitination	E3 ubiquitin ligase	KLHL2	O95198	PMID:22199232

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Function	Reference
382	S	Phosphorylation		-
Autophosphorylation at Ser-382 is inhibited by intracellular calcium.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of WNK1_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
CLH1_HUMAN	CLTC	physical	20936779
FLNC_HUMAN	FLNC	physical	20936779
ATP8_HUMAN	ATP8	physical	20936779
KAP0_HUMAN	PRKAR1A	physical	20936779
KAP1_HUMAN	PRKAR1B	physical	20936779
SL9A1_HUMAN	SLC9A1	physical	20936779
ZYX_HUMAN	ZYX	physical	20936779
CSDE1_HUMAN	CSDE1	physical	20936779
T22D1_HUMAN	TSC22D1	physical	20936779
BAG3_HUMAN	BAG3	physical	20936779
RANB9_HUMAN	RANBP9	physical	20936779
CSN5_HUMAN	COPS5	physical	20936779
TNR6A_HUMAN	TNRC6A	physical	20936779
ZN106_HUMAN	ZNF106	physical	20936779
WNK1_HUMAN	WNK1	physical	20936779
WNK2_HUMAN	WNK2	physical	20936779
SGK1_HUMAN	SGK1	physical	20525693
NED4L_HUMAN	NEDD4L	physical	20525693
SGK1_HUMAN	SGK1	physical	16006511
KLHL3_HUMAN	KLHL3	physical	23387299
NED4L_HUMAN	NEDD4L	physical	26241057
PLEC_HUMAN	PLEC	physical	27173435

Drug and Disease Associations

Kegg Disease
There are no disease associations of PTM sites.
OMIM Disease
614492	Pseudohypoaldosteronism 2C (PHA2C)
201300	Neuropathy, hereditary sensory and autonomic, 2A (HSAN2A)
Kegg Drug
There are no disease associations of PTM sites.
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of WNK1_HUMAN

Related Literatures of Post-Translational Modification

Phosphorylation
Reference	PubMed
"Quantitative phosphoproteomic analysis of T cell receptor signalingreveals system-wide modulation of protein-protein interactions."; Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,Rodionov V., Han D.K.; Sci. Signal. 2:RA46-RA46(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1978 AND SER-2372, ANDMASS SPECTROMETRY.	19690332 [Abstract]
"Lys-N and trypsin cover complementary parts of the phosphoproteome ina refined SCX-based approach."; Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J.,Mohammed S.; Anal. Chem. 81:4493-4501(2009). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-2012 ANDSER-2372, AND MASS SPECTROMETRY.	19413330 [Abstract]
"A quantitative atlas of mitotic phosphorylation."; Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,Elledge S.J., Gygi S.P.; Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-1261; SER-1978;SER-2011; SER-2012; SER-2027; SER-2029; SER-2032 AND SER-2121, ANDMASS SPECTROMETRY.	18669648 [Abstract]
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT forefficient phosphoproteomic analysis."; Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D.,Yates J.R. III; J. Proteome Res. 7:1346-1351(2008). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1297 AND SER-1300, ANDMASS SPECTROMETRY.	18220336 [Abstract]
"ATM and ATR substrate analysis reveals extensive protein networksresponsive to DNA damage."; Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.; Science 316:1160-1166(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-167 AND SER-174, ANDMASS SPECTROMETRY.	17525332 [Abstract]
"Quantitative phosphoproteome profiling of Wnt3a-mediated signalingnetwork: indicating the involvement of ribonucleoside-diphosphatereductase M2 subunit phosphorylation at residue serine 20 in canonicalWnt signal transduction."; Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S.,Li S.-J., Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R.; Mol. Cell. Proteomics 6:1952-1967(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2029 AND SER-2032, ANDMASS SPECTROMETRY.	17693683 [Abstract]
"Improved titanium dioxide enrichment of phosphopeptides from HeLacells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."; Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.; J. Proteome Res. 6:4150-4162(2007). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1978, AND MASSSPECTROMETRY.	17924679 [Abstract]
"A probability-based approach for high-throughput proteinphosphorylation analysis and site localization."; Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.; Nat. Biotechnol. 24:1285-1292(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1261, AND MASSSPECTROMETRY.	16964243 [Abstract]
"Global, in vivo, and site-specific phosphorylation dynamics insignaling networks."; Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P.,Mann M.; Cell 127:635-648(2006). Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2002; SER-2011;SER-2012; SER-2032 AND SER-2038, AND MASS SPECTROMETRY.	17081983 [Abstract]

show