dbPTM

KLHL3_HUMAN - PTM Information in dbPTM

Basic Information of Protein

Overview of Protein Modification Sites with Functional and Structural Information
UniProt ID	KLHL3_HUMAN
UniProt AC	Q9UH77
Protein Name	Kelch-like protein 3
Gene Name	KLHL3 {ECO:0000303\|PubMed:10843806, ECO:0000312\|HGNC:HGNC:6354}
Organism	Homo sapiens (Human).
Sequence Length	587
Subcellular Localization	Cytoplasm, cytoskeleton . Cytoplasm, cytosol .
Protein Description	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. [PubMed: 14528312]
Protein Sequence	MEGESVKLSSQTLIQAGDDEKNQRTITVNPAHMGKAFKVMNELRSKQLLCDVMIVAEDVEIEAHRVVLAACSPYFCAMFTGDMSESKAKKIEIKDVDGQTLSKLIDYIYTAEIEVTEENVQVLLPAASLLQLMDVRQNCCDFLQSQLHPTNCLGIRAFADVHTCTDLLQQANAYAEQHFPEVMLGEEFLSLSLDQVCSLISSDKLTVSSEEKVFEAVISWINYEKETRLEHMAKLMEHVRLPLLPRDYLVQTVEEEALIKNNNTCKDFLIEAMKYHLLPLDQRLLIKNPRTKPRTPVSLPKVMIVVGGQAPKAIRSVECYDFEEDRWDQIAELPSRRCRAGVVFMAGHVYAVGGFNGSLRVRTVDVYDGVKDQWTSIASMQERRSTLGAAVLNDLLYAVGGFDGSTGLASVEAYSYKTNEWFFVAPMNTRRSSVGVGVVEGKLYAVGGYDGASRQCLSTVEQYNPATNEWIYVADMSTRRSGAGVGVLSGQLYATGGHDGPLVRKSVEVYDPGTNTWKQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPVTDKWTLLPTNMSTGRSYAGVAVIHKSL

Experimental Post-Translational Modification Sites

* ASA = Accessible Surface Area

Locations	Modification	Substrate Peptides & Secondary Structure	ASA (%)	Reference
2 (in isoform 3)	Phosphorylation	-	72.21	29116813
4 (in isoform 3)	Phosphorylation	-	38.77	29116813
9	Phosphorylation	EGESVKLSSQTLIQA CCCEEEECCCEEEEC	18.06	23403867
10	Phosphorylation	GESVKLSSQTLIQAG CCEEEECCCEEEECC	37.31	26435498
12	Phosphorylation	SVKLSSQTLIQAGDD EEEECCCEEEECCCC	27.98	23403867
100	Phosphorylation	IKDVDGQTLSKLIDY EEECCCCCHHHHHHH	38.23	-
208	Phosphorylation	SSDKLTVSSEEKVFE HCCCCCCCCHHHHHH	27.14	-
209	Phosphorylation	SDKLTVSSEEKVFEA CCCCCCCCHHHHHHH	45.50	-
252	Phosphorylation	PRDYLVQTVEEEALI CHHHHHHHHCHHHHH	23.54	26074081
291	Phosphorylation	LLIKNPRTKPRTPVS HHCCCCCCCCCCCCC	46.81	-
295	Phosphorylation	NPRTKPRTPVSLPKV CCCCCCCCCCCCCEE	36.25	26435498
375	Phosphorylation	DGVKDQWTSIASMQE CCCHHHHHHHHHHHH	12.48	26435498
376	Phosphorylation	GVKDQWTSIASMQER CCHHHHHHHHHHHHH	17.13	26435498
432	Phosphorylation	APMNTRRSSVGVGVV EECCCCCCCCEEEEE	26.53	24719451
433	Phosphorylation	PMNTRRSSVGVGVVE ECCCCCCCCEEEEEC	22.53	10843806
444	Phosphorylation	GVVEGKLYAVGGYDG EEECCEEEEECCCCC	11.61	24719451
453	Phosphorylation	VGGYDGASRQCLSTV ECCCCCHHHHHHHHH	28.54	24719451
518	"N6,N6-dimethyllysine"	DPGTNTWKQVADMNM CCCCCHHHHHHCHHC	32.62	-
518	Methylation	DPGTNTWKQVADMNM CCCCCHHHHHHCHHC	32.62	-
576	Phosphorylation	TNMSTGRSYAGVAVI CCCCCCCCEEEEEEE	22.22	-
577	Phosphorylation	NMSTGRSYAGVAVIH CCCCCCCEEEEEEEE	13.15	-

Upstream regulatory proteins (kinases for phosphorylation sites, E3 ubiquitin ligases of ubiquitination sites, ...)

Modified Location	Modified Residue	Modification	Type of Upstream Proteins	Gene Name of Upstream Proteins	UniProt AC of Upstream Proteins	Sources
433	S	Phosphorylation	Kinase	PRKACA	P17612	GPS
433	S	Phosphorylation	Kinase	PRKCA	P17252	GPS
433	S	Phosphorylation	Kinase	PKCB	P05771	PSP
433	S	Phosphorylation	Kinase	PKA	-	Uniprot

Functions of PTM Sites

Modified Location	Modified Residue	Modification	Reference
433	S	Phosphorylation	26435498
Phosphorylation at Ser-433 by PKA decreases the interaction with WNK4, Leading to inhibit WNK4 degradation by the BCR(KLHL3) complex.
433	S	Phosphorylation	26435498
Phosphorylation at Ser-433 is increased by insulin.

Disease-associated PTM Sites based on SAP

* Distance = the distance between SAP position and PTM sites.

Modified Location	Modification	Variant Position (Distance <= 10)	Residue Change	SAP	Related Disease	Reference
Oops, there are no SNP-PTM records of KLHL3_HUMAN !!

Protein-Protein Interaction

Interacting Protein	Gene Name	Interaction Type	PPI Reference
WNK1_HUMAN	WNK1	physical	23387299
WNK2_HUMAN	WNK2	physical	23387299
WNK3_HUMAN	WNK3	physical	23387299
CUL3_HUMAN	CUL3	physical	23387299
KLHL3_HUMAN	KLHL3	physical	23387299
RBX1_HUMAN	RBX1	physical	23387299
WNK4_HUMAN	WNK4	physical	23453970
CUL3_HUMAN	CUL3	physical	23573258
CUL3_HUMAN	CUL3	physical	23576762
WNK1_HUMAN	WNK1	physical	23576762
WNK4_HUMAN	WNK4	physical	23576762
WNK1_HUMAN	WNK1	physical	23838290
WNK2_HUMAN	WNK2	physical	23838290
WNK3_HUMAN	WNK3	physical	23838290
WNK4_HUMAN	WNK4	physical	23838290
WNK4_HUMAN	WNK4	physical	23962426
WNK4_HUMAN	WNK4	physical	24641320
WNK3_HUMAN	WNK3	physical	24641320
WNK4_HUMAN	WNK4	physical	25313067
KLHL3_HUMAN	KLHL3	physical	25416956
KLH12_HUMAN	KLHL12	physical	25416956
CF206_HUMAN	C6orf165	physical	25416956
CLD8_HUMAN	CLDN8	physical	25831548
CUL3_HUMAN	CUL3	physical	26286618
WNK1_HUMAN	WNK1	physical	26286618
WNK4_HUMAN	WNK4	physical	26286618
WNK4_HUMAN	WNK4	physical	26349538
SQSTM_HUMAN	SQSTM1	physical	26349538

Drug and Disease Associations

Kegg Drug
DrugBank
There are no disease associations of PTM sites.

Regulatory Network of KLHL3_HUMAN

Related Literatures of Post-Translational Modification